Restriction map for
pFN21AB5675
(Circular) (Six-base) MAPSORT of: pFN21AB5675.seq Check: 183 from: 1 to: 7738
pFN21AB5675 7738 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2211 2704 3223 4716 2211
Size: 493 519 1493 5233
Fragments arranged by size:
5233 1493 519 493
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5548 278
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5081 1937
Size: 3144 4594
Acc65I G'GTAC_C
Cuts at: 5059 5059
Size: 7738
AclI AA'CG_TT
Cuts at: 1563 3848 5865 6238 1563
Size: 2285 2017 373 3063
Fragments arranged by size:
3063 2285 2017 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 3712 3973 4478 5801 7185 7453 1632
Size: 1127 953 261 505 1323 1384 268 1917
Fragments arranged by size:
1917 1384 1323 1127 953 505 268 261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7541
AflIII A'CryG_T
Cuts at: 1203 2982 6637 7614 1203
Size: 1779 3655 977 1327
Fragments arranged by size:
3655 1779 1327 977
AgeI A'CCGG_T
Cuts at: 3170 6569 3170
Size: 3399 4339
AhdI GACnn_n'nnGTC
Cuts at: 6467 6467
Size: 7738
AleI CACnn'nnGTG
Cuts at: 1166 2289 2499 2768 4871 1166
Size: 1123 210 269 2103 4033
Fragments arranged by size:
4033 2103 1123 269 210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 3653 4706 4782 1184
Size: 1234 1235 1053 76 4140
Fragments arranged by size:
4140 1235 1234 1053 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 7706
AlwNI CAG_nnn'CTG
Cuts at: 2701 2836 3504 5481 7053 2701
Size: 135 668 1977 1572 3386
Fragments arranged by size:
3386 1977 1572 668 135
ApaI G_GGCC'C
Cuts at: 4733 4733
Size: 7738
ApaLI G'TGCA_C
Cuts at: 2198 3303 4070 4442 4703 5795 6951 2198
Size: 1105 767 372 261 1092 1156 2985
Fragments arranged by size:
2985 1156 1105 1092 767 372 261
ApoI r'AATT_y
Cuts at: 1493 5047 5273 5425 7534 1493
Size: 3554 226 152 2109 1697
Fragments arranged by size:
3554 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6292 160
Size: 872 5260 1606
Fragments arranged by size:
5260 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2607 2858 4621 4646 5063 1870
Size: 73 202 462 251 1763 25 417 4545
Fragments arranged by size:
4545 1763 462 417 251 202 73 25
BamHI G'GATC_C
Cuts at: 5068 5435 5068
Size: 367 7371
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2388 2442 2501 2661
Size: 325 217 627 222 379 54 59 160
Cuts at: 2661 3356 3751 3977 4128 4524 4631 5059 6514
Size: 695 395 226 151 396 107 428 1455
Cuts at: 6514 618
Size: 1842
Fragments arranged by size:
1842 1455 695 627 428 396 395 379
325 226 222 217 160 151 107 59
54
BanII G_rGCy'C
Cuts at: 1391 2957 4033 4314 4437 4654 4733 5057 1391
Size: 1566 1076 281 123 217 79 324 4072
Fragments arranged by size:
4072 1566 1076 324 281 217 123 79
BbeI G_GCGC'C
Cuts at: 2013 2446 2665 4528 4635 2013
Size: 433 219 1863 107 5116
Fragments arranged by size:
5116 1863 433 219 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4055 928
Size: 971 2156 4611
Fragments arranged by size:
4611 2156 971
BbvCI CC'TCA_GC
Cuts at: 3215 3215
Size: 7738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3310 3344 5929 5963 1903
Size: 34 1373 34 2585 34 3678
Fragments arranged by size:
3678 2585 1373 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2150 5630 6840 2150
Size: 3480 1210 3048
Fragments arranged by size:
3480 3048 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2330 6349 136
Size: 107 122 71 631 605 658 4019 1525
Fragments arranged by size:
4019 1525 658 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7733 7733
Size: 7738
BlpI GC'TnA_GC
Cuts at: 3512 5155 3512
Size: 1643 6095
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3307 3642 4074 4446 4707 4733 5799
Size: 412 1105 335 432 372 261 26 1066
Cuts at: 5799 6955 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1105 1066 432 412 372 335
261 26
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5469 6427 442
Size: 416 53 83 399 24 4052 958 1753
Fragments arranged by size:
4052 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7738
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 4915 2635
Size: 2280 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2561 2723 3269 3317 4154 4235 4283
Size: 1229 93 162 546 48 837 81 48
Cuts at: 4283 4451 4885 6398 1239
Size: 168 434 1513 2579
Fragments arranged by size:
2579 1513 1229 837 546 434 168 162
93 81 48 48
Bpu10I CC'TnA_GC
Cuts at: 3215 4796 3215
Size: 1581 6157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4840 4892 5863 6728 7026 4840
Size: 52 971 865 298 5552
Fragments arranged by size:
5552 971 865 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2418 4856 6401 882
Size: 617 919 2438 1545 2219
Fragments arranged by size:
2438 2219 1545 919 617
BsaAI yAC'GTr
Cuts at: 493 1204 2359 3373 5529 493
Size: 711 1155 1014 2156 2702
Fragments arranged by size:
2702 2156 1155 1014 711
BsaBI GATnn'nnATC
Cuts at: 4352 7732 4352
Size: 3380 4358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2443 2662 3345
Size: 53 83 186 936 477 433 219 683
Cuts at: 3345 4525 4632 5545 5927 6562 275
Size: 1180 107 913 382 635 1451
Fragments arranged by size:
1451 1180 936 913 683 635 477 433
382 219 186 107 83 53
BsaWI w'CCGG_w
Cuts at: 3170 3293 3688 4784 6171 6569 6843 6990 7401
Size: 123 395 1096 1387 398 274 147 411
Cuts at: 7401 3170
Size: 3507
Fragments arranged by size:
3507 1387 1096 411 398 395 274 147
123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2702 2732 3683 3713 4127
Size: 30 165 30 1299 30 951 30 414
Cuts at: 4127 4157 4487 4517 4560 4590 4748 4778 4817
Size: 30 330 30 43 30 158 30 39
Cuts at: 4817 4847 1178
Size: 30 4069
Fragments arranged by size:
4069 1299 951 414 330 165 158 43
39 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4282 4644 1186
Size: 3096 362 4280
Fragments arranged by size:
4280 3096 362
BseYI C'CCAG_C
Cuts at: 2466 2808 3408 3641 3662 4026 4725 6941 7437
Size: 342 600 233 21 364 699 2216 496
Cuts at: 7437 2466
Size: 2767
Fragments arranged by size:
2767 2216 699 600 496 364 342 233
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2315 4082 1163
Size: 573 462 117 1767 4819
Fragments arranged by size:
4819 1767 573 462 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2751 3191 5188 5949 6977 1540
Size: 275 186 750 440 1997 761 1028 2301
Fragments arranged by size:
2301 1997 1028 761 750 440 275 186
BsiHKAI G_wGCw'C
Cuts at: 2146 2202 3307 3333 3837 4074 4437 4446 4707
Size: 56 1105 26 504 237 363 9 261
Cuts at: 4707 4761 5799 5884 6955 2146
Size: 54 1038 85 1071 2929
Fragments arranged by size:
2929 1105 1071 1038 504 363 261 237
85 56 54 26 9
BsmI GAATG_Cn'
Cuts at: 2980 5249 5342 2980
Size: 2269 93 5376
Fragments arranged by size:
5376 2269 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2202 2957 3307 3333 3642 3837
Size: 399 356 56 755 350 26 309 195
Cuts at: 3837 4033 4074 4314 4437 4446 4654 4707 4733
Size: 196 41 240 123 9 208 53 26
Cuts at: 4733 4761 5057 5799 5884 6955 1391
Size: 28 296 742 85 1071 2174
Fragments arranged by size:
2174 1071 755 742 399 356 350 309
296 240 208 196 195 123 85 56
53 41 28 26 26 9
BspEI T'CCGG_A
Cuts at: 3293 3688 4784 7401 3293
Size: 395 1096 2617 3630
Fragments arranged by size:
3630 2617 1096 395
BspHI T'CATG_A
Cuts at: 5627 7357 5627
Size: 1730 6008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2704 3223 4698 4716 5093 844
Size: 1367 493 519 1475 18 377 3489
Fragments arranged by size:
3489 1475 1367 519 493 377 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 3511 4395 5625 1394
Size: 429 275 1413 884 1230 3507
Fragments arranged by size:
3507 1413 1230 884 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 2563 4252 6233 6407 65
Size: 1183 1315 1689 1981 174 1396
Fragments arranged by size:
1981 1689 1396 1315 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 2288 2916 3170 3190 3589 4572
Size: 110 328 150 628 254 20 399 983
Cuts at: 4572 6382 6569 1700
Size: 1810 187 2869
Fragments arranged by size:
2869 1810 983 628 399 328 254 187
150 110 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7738
BssHII G'CGCG_C
Cuts at: 1925 3389 3527 1925
Size: 1464 138 6136
Fragments arranged by size:
6136 1464 138
BssSI C'ACGA_G
Cuts at: 5798 6810 5798
Size: 1012 6726
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 3904 4069 794
Size: 1412 1698 165 4463
Fragments arranged by size:
4463 1698 1412 165
BstBI TT'CG_AA
Cuts at: 6542 6542
Size: 7738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7738
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 3649 4873 2878
Size: 771 1224 5743
Fragments arranged by size:
5743 1224 771
BstYI r'GATC_y
Cuts at: 1972 2335 3800 4035 4257 5068 5435 5820 5837
Size: 363 1465 235 222 811 367 385 17
Cuts at: 5837 7289 7375 7387 7733 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1465 1452 811 385 367 363 346
235 222 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3973 4605 3973
Size: 632 7106
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 2751 2949 4314 4986 513
Size: 552 939 240 507 198 1365 672 3265
Fragments arranged by size:
3265 1365 939 672 552 507 240 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3335 3660 487
Size: 683 56 2109 325 4565
Fragments arranged by size:
4565 2109 683 325 56
BtsI GCAGTG_nn'
Cuts at: 2904 3052 3710 5254 6048 6068 2904
Size: 148 658 1544 794 20 4574
Fragments arranged by size:
4574 1544 794 658 148 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2663 2698 529
Size: 35 2099 35 5569
Fragments arranged by size:
5569 2099 35 35
DraI TTT'AAA
Cuts at: 5042 5042
Size: 7738
DraIII CAC_nnn'GTG
Cuts at: 2941 4045 4534 2941
Size: 1104 489 6145
Fragments arranged by size:
6145 1104 489
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6745 809
Size: 507 5429 1802
Fragments arranged by size:
5429 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2599 2901
Size: 54 1425 50 275 18 260 509 302
Cuts at: 2901 3188 3270 3375 4249 4719 4875 4965 5185
Size: 287 82 105 874 470 156 90 220
Cuts at: 5185 6074 7703 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 874 509 470 302 287
275 260 220 156 105 90 82 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3188 5185 1537
Size: 275 1376 1997 4090
Fragments arranged by size:
4090 1997 1376 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 5668 1353
Size: 1412 2903 3423
Fragments arranged by size:
3423 2903 1412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3577 4598 6325 6699 6845 387
Size: 1064 138 1988 1021 1727 374 146 1280
Fragments arranged by size:
1988 1727 1280 1064 1021 374 146 138
EcoICRI GAG'CTC
Cuts at: 4435 4435
Size: 7738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2561 2723 2759 3269 3317 3712
Size: 393 836 93 162 36 510 48 395
Cuts at: 3712 3973 4154 4235 4283 4451 4478 4885 5801
Size: 261 181 81 48 168 27 407 916
Cuts at: 5801 6398 7185 7453 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 916 836 787 597 510 407 395
393 268 261 181 168 162 93 81
48 48 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3314 3861 3314
Size: 547 7191
EcoO109I rG'GnC_Cy
Cuts at: 2063 2845 3281 3413 3619 4594 4687 4729 4730
Size: 782 436 132 206 975 93 42 1
Cuts at: 4730 4825 4928 2063
Size: 95 103 4873
Fragments arranged by size:
4873 975 782 436 206 132 103 95
93 42 1
EcoRI G'AATT_C
Cuts at: 5047 5425 5047
Size: 378 7360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4072 4246 4072
Size: 174 7564
FseI GG_CCGG'CC
Cuts at: 3194 3194
Size: 7738
FspI TGC'GCA
Cuts at: 2631 3027 4682 6244 2631
Size: 396 1655 1562 4125
Fragments arranged by size:
4125 1655 1562 396
HaeII r_GCGC'y
Cuts at: 1678 2013 2446 2665 3214 3257 4528 4635 4803
Size: 335 433 219 549 43 1271 107 168
Cuts at: 4803 6885 1678
Size: 2082 2531
Fragments arranged by size:
2531 2082 1271 549 433 335 219 168
107 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3956 3988 4487 4519 4560
Size: 32 135 32 2384 32 499 32 41
Cuts at: 4560 4592 4817 4849 6379 6411 6453 6485 1373
Size: 32 225 32 1530 32 42 32 2626
Fragments arranged by size:
2626 2384 1530 499 225 135 42 41
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4339 5082 5328 669
Size: 988 281 2401 743 246 3079
Fragments arranged by size:
3079 2401 988 743 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7738
HpaI GTT'AAC
Cuts at: 5328 5328
Size: 7738
KasI G'GCGC_C
Cuts at: 2009 2442 2661 4524 4631 2009
Size: 433 219 1863 107 5116
Fragments arranged by size:
5116 1863 433 219 107
KpnI G_GTAC'C
Cuts at: 5063 5063
Size: 7738
MfeI C'AATT_G
Cuts at: 5337 5337
Size: 7738
MluI A'CGCG_T
Cuts at: 7614 7614
Size: 7738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6852 7036 1305
Size: 5547 184 2007
Fragments arranged by size:
5547 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 3377 4721 4877 10
Size: 54 1425 343 1071 474 1344 156 2871
Fragments arranged by size:
2871 1425 1344 1071 474 343 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 2289 2357 2499 2768 2942 4871
Size: 648 1051 72 68 142 269 174 1929
Cuts at: 4871 5696 6055 6214 518
Size: 825 359 159 2042
Fragments arranged by size:
2042 1929 1051 825 648 359 269 174
159 142 72 68
MspA1I CmG'CkG
Cuts at: 1606 2753 2836 3016 3247 3354 4012 4026 4126
Size: 1147 83 180 231 107 658 14 100
Cuts at: 4126 4213 4375 4725 4957 5154 5831 6979 7224
Size: 87 162 350 232 197 677 1148 245
Cuts at: 7224 1606
Size: 2120
Fragments arranged by size:
2120 1148 1147 677 658 350 245 232
231 197 180 162 107 100 87 83
14
NaeI GCC'GGC
Cuts at: 3192 3192
Size: 7738
NarI GG'CG_CC
Cuts at: 2010 2443 2662 4525 4632 2010
Size: 433 219 1863 107 5116
Fragments arranged by size:
5116 1863 433 219 107
NcoI C'CATG_G
Cuts at: 513 1065 2004 2949 4986 513
Size: 552 939 945 2037 3265
Fragments arranged by size:
3265 2037 945 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7738
NgoMIV G'CCGG_C
Cuts at: 3190 3190
Size: 7738
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7738
NotI GC'GGCC_GC
Cuts at: 5185 5185
Size: 7738
NspI r_CATG'y
Cuts at: 1132 1229 2169 2191 2986 4372 5096 6641 1132
Size: 97 940 22 795 1386 724 1545 2229
Fragments arranged by size:
2229 1545 1386 940 795 724 97 22
PasI CC'CwG_GG
Cuts at: 1348 2911 3556 3721 4399 1348
Size: 1563 645 165 678 4687
Fragments arranged by size:
4687 1563 678 645 165
PciI A'CATG_T
Cuts at: 2982 6637 2982
Size: 3655 4083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2985 3373 1108
Size: 1877 388 5473
Fragments arranged by size:
5473 1877 388
PfoI T'CCnGG_A
Cuts at: 2304 4260 2304
Size: 1956 5782
PmeI GTTT'AAAC
Cuts at: 5042 5042
Size: 7738
PmlI CAC'GTG
Cuts at: 3373 3373
Size: 7738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 5807 5839 7320 7352 3683
Size: 32 2092 32 1481 32 4069
Fragments arranged by size:
4069 2092 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2845 3281 3619 4594 4687 4825 2845
Size: 436 338 975 93 138 5758
Fragments arranged by size:
5758 975 436 338 138 93
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7738
PsiI TTA'TAA
Cuts at: 3549 5308 3549
Size: 1759 5979
PspOMI G'GGCC_C
Cuts at: 4729 4729
Size: 7738
PstI C_TGCA'G
Cuts at: 830 3234 4561 4860 5090 830
Size: 2404 1327 299 230 3478
Fragments arranged by size:
3478 2404 1327 299 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7738
PvuII CAG'CTG
Cuts at: 3016 3247 4375 4725 4957 3016
Size: 231 1128 350 232 5797
Fragments arranged by size:
5797 1128 350 232 231
SacI G_AGCT'C
Cuts at: 4437 4437
Size: 7738
SacII CC_GC'GG
Cuts at: 2754 2754
Size: 7738
SalI G'TCGA_C
Cuts at: 1936 5080 1936
Size: 3144 4594
SanDI GG'GwC_CC
Cuts at: 3281 3281
Size: 7738
SbfI CC_TGCA'GG
Cuts at: 3234 4860 5090 3234
Size: 1626 230 5882
Fragments arranged by size:
5882 1626 230
ScaI AGT'ACT
Cuts at: 2255 5986 7698 2255
Size: 3731 1712 2295
Fragments arranged by size:
3731 2295 1712
SexAI A'CCwGG_T
Cuts at: 3110 4041 4530 3110
Size: 931 489 6318
Fragments arranged by size:
6318 931 489
SfcI C'TryA_G
Cuts at: 826 1045 3230 4557 4856 5086 6221 6902 7093
Size: 219 2185 1327 299 230 1135 681 191
Cuts at: 7093 826
Size: 1471
Fragments arranged by size:
2185 1471 1327 1135 681 299 230 219
191
SfoI GGC'GCC
Cuts at: 2011 2444 2663 4526 4633 2011
Size: 433 219 1863 107 5116
Fragments arranged by size:
5116 1863 433 219 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
SgrAI Cr'CCGG_yG
Cuts at: 1700 2288 1700
Size: 588 7150
SmaI CCC'GGG
Cuts at: 1872 4623 5065 1872
Size: 2751 442 4545
Fragments arranged by size:
4545 2751 442
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4819 4907 5842 6743 7005 820
Size: 197 926 2876 88 935 901 262 1553
Fragments arranged by size:
2876 1553 935 926 901 262 197 88
SnaBI TAC'GTA
Cuts at: 493 5529 493
Size: 5036 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7738
SphI G_CATG'C
Cuts at: 1132 5096 1132
Size: 3964 3774
SspI AAT'ATT
Cuts at: 5 52 3657 5662 5
Size: 47 3605 2005 2081
Fragments arranged by size:
3605 2081 2005 47
StuI AGG'CCT
Cuts at: 1517 2618 3971 1517
Size: 1101 1353 5284
Fragments arranged by size:
5284 1353 1101
StyI C'CwwG_G
Cuts at: 513 1065 2004 2795 2871 2949 4056 4986 5177
Size: 552 939 791 76 78 1107 930 191
Cuts at: 5177 513
Size: 3074
Fragments arranged by size:
3074 1107 939 930 791 552 191 78
76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 2458 2507 4504 5765 5782 5935
Size: 1255 722 156 49 1997 1261 17 153
Cuts at: 5935 6120 6565 325
Size: 185 445 1498
Fragments arranged by size:
1997 1498 1261 1255 722 445 185 156
153 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2298 2862
Size: 274 80 33 51 1443 276 45 564
Cuts at: 2862 5984 7696 96
Size: 3122 1712 138
Fragments arranged by size:
3122 1712 1443 564 276 274 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2702 2734 2804 2836 3774 3806 4127 4159 7698
Size: 32 70 32 938 32 321 32 3539
Cuts at: 7698 7730 2702
Size: 32 2710
Fragments arranged by size:
3539 2710 938 321 70 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2074 2312 3347 1316
Size: 758 238 1035 5707
Fragments arranged by size:
5707 1035 758 238
XbaI T'CTAG_A
Cuts at: 5074 5074
Size: 7738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2522 4975 2258
Size: 264 2453 5021
Fragments arranged by size:
5021 2453 264
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7738
XmaI C'CCGG_G
Cuts at: 1870 4621 5063 1870
Size: 2751 442 4545
Fragments arranged by size:
4545 2751 442
XmnI GAAnn'nnTTC
Cuts at: 5867 5867
Size: 7738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5546 276
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII
SapI SfiI SrfI SwaI