Restriction map for
pFN21AB5667
(Circular) (Six-base) MAPSORT of: pFN21AB5667.seq Check: 5338 from: 1 to: 5716
pFN21AB5667 5716 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 3779 5163 5431 1632
Size: 668 1479 1384 268 1917
Fragments arranged by size:
1917 1479 1384 668 268
AfeI AGC'GCT
Cuts at: 2073 2073
Size: 5716
AflII C'TTAA_G
Cuts at: 820 1017 2741 820
Size: 197 1724 3795
Fragments arranged by size:
3795 1724 197
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2954 1184
Size: 1770 3946
AlwNI CAG_nnn'CTG
Cuts at: 2029 3459 5031 2029
Size: 1430 1572 2714
Fragments arranged by size:
2714 1572 1430
ApaI G_GGCC'C
Cuts at: 2129 2188 2806 2129
Size: 59 618 5039
Fragments arranged by size:
5039 618 59
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2321 2515 3025 3251 3403 5512 1493
Size: 828 194 510 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 510 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2210 2642 3037 4492
Size: 325 217 627 258 165 432 395 1455
Cuts at: 4492 618
Size: 1842
Fragments arranged by size:
1842 1455 627 432 395 325 258 217
165
BanII G_rGCy'C
Cuts at: 1391 2129 2188 2371 2806 3035 1391
Size: 738 59 183 435 229 4072
Fragments arranged by size:
4072 738 435 229 183 59
BbeI G_GCGC'C
Cuts at: 2214 2214
Size: 5716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4745
BbvCI CC'TCA_GC
Cuts at: 2478 2478
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BfrBI ATG'CAT
Cuts at: 3001 3001
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 2924 4327
Size: 107 122 71 631 605 577 675 1403
Cuts at: 4327 136
Size: 1525
Fragments arranged by size:
1525 1403 675 631 605 577 122 107
71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2063 2129 2188 2806 2875 3777 4933
Size: 258 15 66 59 618 69 902 1156
Cuts at: 4933 1790
Size: 2573
Fragments arranged by size:
2573 1156 902 618 258 69 66 59
15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 2851 3447
Size: 416 53 83 399 24 683 751 596
Cuts at: 3447 4405 442
Size: 958 1753
Fragments arranged by size:
1753 958 751 683 596 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 2908 2079
Size: 829 4887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4376 1239
Size: 3137 2579
Bpu10I CC'TnA_GC
Cuts at: 2478 2478
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 3523 3905 4540
Size: 53 83 186 936 678 1312 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1312 936 678 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 2503 1186
Size: 1089 228 4399
Fragments arranged by size:
4399 1089 228
BseYI C'CCAG_C
Cuts at: 2981 4919 5415 2981
Size: 1938 496 3282
Fragments arranged by size:
3282 1938 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3777 3862 4933 3777
Size: 85 1071 4560
Fragments arranged by size:
4560 1071 85
BsmI GAATG_Cn'
Cuts at: 3001 3227 3320 3001
Size: 226 93 5397
Fragments arranged by size:
5397 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2063 2129 2188 2371 2806 2875
Size: 399 258 15 66 59 183 435 69
Cuts at: 2875 3035 3777 3862 4933 1391
Size: 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 258 183 160
85 69 66 59 15
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2902 3603 1394
Size: 429 1079 701 3507
Fragments arranged by size:
3507 1079 701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 4360 4547 1700
Size: 110 406 2144 187 2869
Fragments arranged by size:
2869 2144 406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 2504 2863 794
Size: 1235 475 359 3647
Fragments arranged by size:
3647 1235 475 359
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 2244 2253 2344 513
Size: 552 939 93 147 9 91 3885
Fragments arranged by size:
3885 939 552 147 93 91 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 3232 4026 4046 2235
Size: 997 794 20 3905
Fragments arranged by size:
3905 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 3163 4052
Size: 54 1425 50 275 18 460 873 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 873 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 3646 1353
Size: 1211 1082 3423
Fragments arranged by size:
3423 1211 1082
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 3779 4376 5163 5431 1239
Size: 393 668 1479 597 787 268 1524
Fragments arranged by size:
1524 1479 787 668 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2126 2184 2185 2584 2803 2126
Size: 58 1 399 219 5039
Fragments arranged by size:
5039 399 219 58 1
EcoRI G'AATT_C
Cuts at: 2321 2515 3025 3403 2321
Size: 194 510 378 4634
Fragments arranged by size:
4634 510 378 194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2984 2984
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2075 2214 4863 1678
Size: 397 139 2649 2531
Fragments arranged by size:
2649 2531 397 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373
Size: 32 135 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2210 2210
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 2667 2691 3033 4830 5014 1305
Size: 1116 246 24 342 1797 184 2007
Fragments arranged by size:
2007 1797 1116 342 246 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 3674 4033 4192 518
Size: 648 1564 944 359 159 2042
Fragments arranged by size:
2042 1564 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2052 2099 2170 3132 3809 4957 5202
Size: 420 26 47 71 962 677 1148 245
Cuts at: 5202 1606
Size: 2120
Fragments arranged by size:
2120 1148 962 677 420 245 71 47
26
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 5716
NarI GG'CG_CC
Cuts at: 2211 2211
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4225
Fragments arranged by size:
4225 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NsiI A_TGCA'T
Cuts at: 3003 3003
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 1108
Size: 1722 3994
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2125 2184 2802 2125
Size: 59 618 5039
Fragments arranged by size:
5039 618 59
PstI C_TGCA'G
Cuts at: 830 2034 3068 830
Size: 1204 1034 3478
Fragments arranged by size:
3478 1204 1034
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SacII CC_GC'GG
Cuts at: 2100 2100
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 2030 3064 4199 4880 5071 826
Size: 219 985 1034 1135 681 191 1471
Fragments arranged by size:
1471 1135 1034 985 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2249 2249
Size: 5716
SfoI GGC'GCC
Cuts at: 2212 2212
Size: 5716
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 3820 4721 4983 820
Size: 197 926 798 1079 901 262 1553
Fragments arranged by size:
1553 1079 926 901 798 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2004 2835 3155 513
Size: 552 939 831 320 3074
Fragments arranged by size:
3074 939 831 552 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2833 3743 3760 3913 4098 4543 325
Size: 1255 1253 910 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1253 910 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3962 5674 96
Size: 274 80 33 51 1443 1985 1712 138
Fragments arranged by size:
1985 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 2879 3052 2879
Size: 173 5543
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SrfI SwaI XcmI