Restriction map for
pFN21AB5663
(Circular) (Six-base) MAPSORT of: pFN21AB5663.seq Check: 3199 from: 1 to: 5590
pFN21AB5663 5590 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3653 5037 5305 1632
Size: 488 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 4489 5466 1203
Size: 3286 977 1327
Fragments arranged by size:
3286 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2579 2870 1166
Size: 1413 291 3886
Fragments arranged by size:
3886 1413 291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 2001
Size: 32 5558
AlwNI CAG_nnn'CTG
Cuts at: 2020 2308 3333 4905 2020
Size: 288 1025 1572 2705
Fragments arranged by size:
2705 1572 1025 288
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2405 2915 1870
Size: 73 462 510 4545
Fragments arranged by size:
4545 510 462 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2911 4366 618
Size: 325 217 627 1124 1455 1842
Fragments arranged by size:
1842 1455 1124 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2663 2909 1391
Size: 892 380 246 4072
Fragments arranged by size:
4072 892 380 246
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4291
Fragments arranged by size:
4291 971 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 2712 1548
Size: 1164 4426
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 3651 4807 1790
Size: 1861 1156 2573
Fragments arranged by size:
2573 1861 1156
BmgBI CAC'GTC
Cuts at: 1318 2872 2881 1318
Size: 1554 9 4027
Fragments arranged by size:
4027 1554 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2297 2689 2789 4250 1239
Size: 980 78 392 100 1461 2579
Fragments arranged by size:
2579 1461 980 392 100 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 3715 4580 4878 2432
Size: 1283 865 298 3144
Fragments arranged by size:
3144 1283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2342 4253 882
Size: 617 606 237 1911 2219
Fragments arranged by size:
2219 1911 617 606 237
BsaAI yAC'GTr
Cuts at: 493 1204 3381 493
Size: 711 2177 2702
Fragments arranged by size:
2702 2177 711
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2001 2031 2483 2513 1178
Size: 30 165 30 598 30 452 30 4255
Fragments arranged by size:
4255 598 452 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2123 2240 2243 2423 2534 2671 2834
Size: 874 63 117 3 180 111 137 163
Cuts at: 2834 2837 2846 2849 2852 2855 1186
Size: 3 9 3 3 3 3921
Fragments arranged by size:
3921 874 180 163 137 117 111 63
9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2308 2790 4793 5289 2308
Size: 482 2003 496 2609
Fragments arranged by size:
2609 2003 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4585
Fragments arranged by size:
4585 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2080 2367 3040 3801 4829 1540
Size: 275 186 79 287 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 287 275 186 79
BsiHKAI G_wGCw'C
Cuts at: 3651 3736 4807 3651
Size: 85 1071 4434
Fragments arranged by size:
4434 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2663 2909 3651 3736 4807 1391
Size: 399 493 380 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 380 246 85
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2123 2945 844
Size: 1279 822 3489
Fragments arranged by size:
3489 1279 822
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2472 4085 4259 65
Size: 1183 1224 1613 174 1396
Fragments arranged by size:
1613 1396 1224 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 2118 1925
Size: 193 5397
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585
Size: 948 367 385 17 1452 86 12 346
Cuts at: 5585 1972
Size: 1977
Fragments arranged by size:
1977 1452 948 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2654 2654
Size: 5590
BtgI C'CryG_G
Cuts at: 513 1065 2004 2607 513
Size: 552 939 603 3496
Fragments arranged by size:
3496 939 603 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 3106 3900 3920 2643
Size: 463 794 20 4313
Fragments arranged by size:
4313 794 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5555
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2364 2460
Size: 54 1425 50 275 18 414 120 96
Cuts at: 2460 2631 3037 3926 5555 8
Size: 171 406 889 1629 43
Fragments arranged by size:
1629 1425 889 414 406 275 171 120
96 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2364 3037 1537
Size: 275 552 673 4090
Fragments arranged by size:
4090 673 552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2823 3520 1353
Size: 1470 697 3423
Fragments arranged by size:
3423 1470 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 2120 2531 2589 4177 4551
Size: 1064 138 444 87 411 58 1588 374
Cuts at: 4551 4697 387
Size: 146 1280
Fragments arranged by size:
1588 1280 1064 444 411 374 146 138
87 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2219 2297 2689 2789 3653 4250
Size: 393 488 99 78 392 100 864 597
Cuts at: 4250 5037 5305 1239
Size: 787 268 1524
Fragments arranged by size:
1524 864 787 597 488 393 392 268
100 99 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2783 2783
Size: 5590
EcoO109I rG'GnC_Cy
Cuts at: 2158 2158
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 4737 1678
Size: 3059 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 10
Size: 54 1425 343 414 3354
Fragments arranged by size:
3354 1425 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2321 2327 2579 2870 3548 3907
Size: 648 1026 129 6 252 291 678 359
Cuts at: 3907 4066 518
Size: 159 2042
Fragments arranged by size:
2042 1026 678 648 359 291 252 159
129 6
MspA1I CmG'CkG
Cuts at: 1606 2242 2305 2618 3006 3683 4831 5076 1606
Size: 636 63 313 388 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 388 313 245 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 2607 513
Size: 552 939 603 3496
Fragments arranged by size:
3496 939 603 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2326 2948 4493 1132
Size: 97 1097 622 1545 2229
Fragments arranged by size:
2229 1545 1097 622 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2001 2033 2481 2513 3659 3691 5172 5204 2001
Size: 32 448 32 1146 32 1481 32 2387
Fragments arranged by size:
2387 1481 1146 448 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2158
Size: 5590
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2304 2942 830
Size: 1474 638 3478
Fragments arranged by size:
3478 1474 638
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2305 2305
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2300 2938 4073 4754 4945 826
Size: 219 1255 638 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 681 638 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 3694 4595 4857 820
Size: 197 926 504 1247 901 262 1553
Fragments arranged by size:
1553 1247 926 901 504 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 2646 1517
Size: 1129 4461
StyI C'CwwG_G
Cuts at: 513 1065 2004 2607 2808 3029 513
Size: 552 939 603 201 221 3074
Fragments arranged by size:
3074 939 603 552 221 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 3617 3634 3787 3972 4417 325
Size: 1255 1298 739 17 153 185 445 1498
Fragments arranged by size:
1498 1298 1255 739 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3836 5548 96
Size: 274 80 33 51 1443 1859 1712 138
Fragments arranged by size:
1859 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2483 2515 5550 5582 2204
Size: 32 247 32 3035 32 2212
Fragments arranged by size:
3035 2212 247 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2254 2194
Size: 60 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI