Restriction map for
pFN21AB5659
(Circular) (Six-base) MAPSORT of: pFN21AB5659.seq Check: 651 from: 1 to: 5629
pFN21AB5659 5629 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2374 3439 278
Size: 53 83 186 936 838 1065 2468
Fragments arranged by size:
2468 1065 936 838 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2972 1937
Size: 1035 4594
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2531 3692 5076 5344 1632
Size: 899 1161 1384 268 1917
Fragments arranged by size:
1917 1384 1161 899 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2265 1184
Size: 1081 4548
AlwNI CAG_nnn'CTG
Cuts at: 2389 3372 4944 2389
Size: 983 1572 3074
Fragments arranged by size:
3074 1572 983
ApaI G_GGCC'C
Cuts at: 2830 2830
Size: 5629
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2504 2938 3164 3316 5425 1493
Size: 1011 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 1011 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2264 2959 3326 2264
Size: 695 367 4567
Fragments arranged by size:
4567 695 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 4405 618
Size: 325 217 627 1163 1455 1842
Fragments arranged by size:
1842 1455 1163 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2367 2830 2874 2948 1391
Size: 976 463 44 74 4072
Fragments arranged by size:
4072 976 463 74 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2235 2269 3820 3854 1903
Size: 34 298 34 1551 34 3678
Fragments arranged by size:
3678 1551 298 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3521 4731 2565
Size: 956 1210 3463
Fragments arranged by size:
3463 1210 956
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2091 5624 2091
Size: 3533 2096
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2707 2830 3690 4846 1790
Size: 917 123 860 1156 2573
Fragments arranged by size:
2573 1156 917 860 123
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2327 3360 4318
Size: 416 53 83 399 24 910 1033 958
Cuts at: 4318 442
Size: 1753
Fragments arranged by size:
1753 1033 958 910 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2482 2729 4289 1239
Size: 1243 247 1560 2579
Fragments arranged by size:
2579 1560 1243 247
Bpu10I CC'TnA_GC
Cuts at: 2075 2582 2075
Size: 507 5122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 3754 4619 4917 2645
Size: 1109 865 298 3357
Fragments arranged by size:
3357 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2732 4292 882
Size: 617 1233 1560 2219
Fragments arranged by size:
2219 1560 1233 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2371 3436 3818 4453
Size: 53 83 186 936 838 1065 382 635
Cuts at: 4453 275
Size: 1451
Fragments arranged by size:
1451 1065 936 838 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2858 2861 1186
Size: 1672 3 3954
Fragments arranged by size:
3954 1672 3
BseYI C'CCAG_C
Cuts at: 4832 5328 4832
Size: 496 5133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 1163
Size: 573 415 4641
Fragments arranged by size:
4641 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2122 3079 3840 4868 1540
Size: 275 186 48 73 957 761 1028 2301
Fragments arranged by size:
2301 1028 957 761 275 186 73 48
BsiHKAI G_wGCw'C
Cuts at: 2367 2874 2918 3690 3775 4846 2367
Size: 507 44 772 85 1071 3150
Fragments arranged by size:
3150 1071 772 507 85 44
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2707 2830 2874 2918 2948 3690
Size: 399 577 340 123 44 44 30 742
Cuts at: 3690 3775 4846 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 399 340 123 85
44 44 30
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2180 4124 4298 65
Size: 1183 932 1944 174 1396
Fragments arranged by size:
1944 1396 1183 932 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 4273 4460 1700
Size: 110 307 2156 187 2869
Fragments arranged by size:
2869 2156 307 187 110
BsrGI T'GTAC_A
Cuts at: 96 2789 96
Size: 2693 2936
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2415 2415
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2091 2264 2959 3326 3711 3728 5180 5266
Size: 119 173 695 367 385 17 1452 86
Cuts at: 5266 5278 5624 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 695 385 367 346 173 119
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 3145 3939 3959 2293
Size: 852 794 20 3963
Fragments arranged by size:
3963 852 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 3076 3965
Size: 54 1425 50 275 18 289 957 889
Cuts at: 3965 5594 8
Size: 1629 43
Fragments arranged by size:
1629 1425 957 889 289 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 3076 1537
Size: 275 307 957 4090
Fragments arranged by size:
4090 957 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3559 1353
Size: 711 1495 3423
Fragments arranged by size:
3423 1495 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2365 2872 2365
Size: 507 5122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2482 2531 2729 3692 4289 5076 5344
Size: 393 850 49 198 963 597 787 268
Cuts at: 5344 1239
Size: 1524
Fragments arranged by size:
1524 963 850 787 597 393 268 198
49
EcoO109I rG'GnC_Cy
Cuts at: 2826 2826
Size: 5629
EcoRI G'AATT_C
Cuts at: 2504 2938 3316 2504
Size: 434 378 4817
Fragments arranged by size:
4817 434 378
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 4776 1678
Size: 3098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2256 2288 2294 2326 2361
Size: 32 135 32 684 32 6 32 35
Cuts at: 2361 2393 4270 4302 4344 4376 1373
Size: 32 1877 32 42 32 2626
Fragments arranged by size:
2626 1877 684 135 42 35 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2392 4743 4927 1305
Size: 1071 16 2351 184 2007
Fragments arranged by size:
2351 2007 1071 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3807
Fragments arranged by size:
3807 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 3587 3946 4105 518
Size: 648 1081 1340 359 159 2042
Fragments arranged by size:
2042 1340 1081 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2760 2894 3045 3722 4870 5115 1606
Size: 1154 134 151 677 1148 245 2120
Fragments arranged by size:
2120 1154 1148 677 245 151 134
NaeI GCC'GGC
Cuts at: 2119 2119
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5077
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NgoMIV G'CCGG_C
Cuts at: 2117 2117
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2987 4532 1132
Size: 97 1758 1545 2229
Fragments arranged by size:
2229 1758 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5629
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 1108
Size: 1281 4348
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5629
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PspOMI G'GGCC_C
Cuts at: 2826 2826
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2894 2894
Size: 5629
SacI G_AGCT'C
Cuts at: 2367 2874 2367
Size: 507 5122
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SapI GCTCTTCn'nnn_
Cuts at: 2064 2064
Size: 5629
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2437 2977 4112 4793 4984 826
Size: 219 1392 540 1135 681 191 1471
Fragments arranged by size:
1471 1392 1135 681 540 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 3733 4634 4896 820
Size: 197 926 717 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 717 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 2545 3553 5
Size: 47 2493 1008 2081
Fragments arranged by size:
2493 2081 1008 47
StuI AGG'CCT
Cuts at: 1517 2194 1517
Size: 677 4952
StyI C'CwwG_G
Cuts at: 513 1065 3068 513
Size: 552 2003 3074
Fragments arranged by size:
3074 2003 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 3656 3673 3826 4011 4456 325
Size: 1255 1176 900 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1176 900 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2789 3875 5587
Size: 274 80 33 51 1443 812 1086 1712
Cuts at: 5587 96
Size: 138
Fragments arranged by size:
1712 1443 1086 812 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5589 5621 5589
Size: 32 5597
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2372 3437 276
Size: 53 83 186 936 838 1065 2468
Fragments arranged by size:
2468 1065 936 838 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI