Restriction map for
pFN21AB5654
(Circular) (Six-base) MAPSORT of: pFN21AB5654.seq Check: 7861 from: 1 to: 5698
pFN21AB5654 5698 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2152 3508 278
Size: 53 83 186 936 616 1356 2468
Fragments arranged by size:
2468 1356 936 616 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2802 3041 1937
Size: 865 239 4594
Fragments arranged by size:
4594 865 239
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2409 2480 3761 5145 5413 1632
Size: 777 71 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 777 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2672 4597 5574 1203
Size: 1469 1925 977 1327
Fragments arranged by size:
1925 1469 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2503 2750 3441 5013 2503
Size: 247 691 1572 3188
Fragments arranged by size:
3188 1572 691 247
ApaI G_GGCC'C
Cuts at: 2446 2446
Size: 5698
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 3017 1391
Size: 1055 571 4072
Fragments arranged by size:
4072 1055 571
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2446 2497 3759 4915 1790
Size: 656 51 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 656 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2738 3429 4387
Size: 416 53 83 399 24 1321 691 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1321 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2976 1056
Size: 1920 3778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2386 4358 1239
Size: 977 170 1972 2579
Fragments arranged by size:
2579 1972 977 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2149 3505 3887 4522
Size: 53 83 186 936 616 1356 382 635
Cuts at: 4522 275
Size: 1451
Fragments arranged by size:
1451 1356 936 635 616 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 1186
Size: 843 4855
BseYI C'CCAG_C
Cuts at: 2297 2421 2901 4901 5397 2297
Size: 124 480 2000 496 2598
Fragments arranged by size:
2598 2000 496 480 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2039 3759 3844 4915 2028
Size: 11 1720 85 1071 2811
Fragments arranged by size:
2811 1720 1071 85 11
BsmI GAATG_Cn'
Cuts at: 2200 2217 3209 3302 2200
Size: 17 992 93 4596
Fragments arranged by size:
4596 992 93 17
BsmBI CGTCTCn'nnnn_
Cuts at: 2156 2156
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2039 2446 2497 3017 3759 3844
Size: 399 238 11 407 51 520 742 85
Cuts at: 3844 4915 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 520 407 399 238 85
51 11
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2556 3587 5317 2556
Size: 1031 1730 2937
Fragments arranged by size:
2937 1730 1031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 4193 4367 65
Size: 1183 1303 1642 174 1396
Fragments arranged by size:
1642 1396 1303 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2915 2915
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2065 3028 3395 3780 3797 5249 5335 5347
Size: 93 963 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 93 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2628 513
Size: 552 1563 3583
Fragments arranged by size:
3583 1563 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 3214 4008 4028 2052
Size: 1162 794 20 3722
Fragments arranged by size:
3722 1162 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2730 3145 4034
Size: 54 1425 50 275 18 900 415 889
Cuts at: 4034 5663 8
Size: 1629 43
Fragments arranged by size:
1629 1425 900 889 415 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3628 1353
Size: 2275 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2386 2409 2480 3761 4358 5145
Size: 393 584 170 23 71 1281 597 787
Cuts at: 5145 5413 1239
Size: 268 1524
Fragments arranged by size:
1524 1281 787 597 584 393 268 170
71 23
EcoO109I rG'GnC_Cy
Cuts at: 2442 2443 2442
Size: 1 5697
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 2697 2729 4339
Size: 32 135 32 567 32 526 32 1610
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1610 567 526 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 10
Size: 54 1425 343 900 2976
Fragments arranged by size:
2976 1425 900 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2458 2901 3114 3791 4939 5184 1606
Size: 852 443 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 677 443 245 213
NcoI C'CATG_G
Cuts at: 513 1065 2628 513
Size: 552 1563 3583
Fragments arranged by size:
3583 1563 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 2972 1052
Size: 1920 3778
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2676 3056 4601 1132
Size: 97 1447 380 1545 2229
Fragments arranged by size:
2229 1545 1447 380 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 2672 4597 2672
Size: 1925 3773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2304 2503 1108
Size: 1196 199 4303
Fragments arranged by size:
4303 1196 199
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2442 2442
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2047 3050 830
Size: 1217 1003 3478
Fragments arranged by size:
3478 1217 1003
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2458 2901 2458
Size: 443 5255
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SexAI A'CCwGG_T
Cuts at: 2502 2502
Size: 5698
SfcI C'TryA_G
Cuts at: 826 1045 2043 2788 3046 4181 4862 5053 826
Size: 219 998 745 258 1135 681 191 1471
Fragments arranged by size:
1471 1135 998 745 681 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2628 3137 513
Size: 552 1563 509 3074
Fragments arranged by size:
3074 1563 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 2682 2819
Size: 274 80 33 51 1443 597 108 137
Cuts at: 2819 2842 3944 5656 96
Size: 23 1102 1712 138
Fragments arranged by size:
1712 1443 1102 597 274 138 137 108
80 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2466 2498 2677 2709 5658 5690 2466
Size: 32 179 32 2949 32 2474
Fragments arranged by size:
2949 2474 179 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2150 3506 276
Size: 53 83 186 936 616 1356 2468
Fragments arranged by size:
2468 1356 936 616 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI