Restriction map for
pFN21AB5648
(Circular) (Six-base) MAPSORT of: pFN21AB5648.seq Check: 3774 from: 1 to: 7183
pFN21AB5648 7183 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4993 278
Size: 53 83 186 936 3457 2468
Fragments arranged by size:
3457 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2737 4345 4526 1937
Size: 800 1608 181 4594
Fragments arranged by size:
4594 1608 800 181
Acc65I G'GTAC_C
Cuts at: 4504 4504
Size: 7183
AclI AA'CG_TT
Cuts at: 1563 4482 5310 5683 1563
Size: 2919 828 373 3063
Fragments arranged by size:
3063 2919 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 3087 3648 5246 6630 6898 1632
Size: 495 960 561 1598 1384 268 1917
Fragments arranged by size:
1917 1598 1384 960 561 495 268
AflII C'TTAA_G
Cuts at: 820 1017 3173 820
Size: 197 2156 4830
Fragments arranged by size:
4830 2156 197
AflIII A'CryG_T
Cuts at: 1203 4071 4264 6082 7059 1203
Size: 2868 193 1818 977 1327
Fragments arranged by size:
2868 1818 1327 977 193
AgeI A'CCGG_T
Cuts at: 3091 6014 3091
Size: 2923 4260
AhdI GACnn_n'nnGTC
Cuts at: 3501 5912 3501
Size: 2411 4772
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7183
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4090 4122 4090
Size: 32 7151
AlwNI CAG_nnn'CTG
Cuts at: 3215 3263 4109 4926 6498 3215
Size: 48 846 817 1572 3900
Fragments arranged by size:
3900 1572 846 817 48
ApaLI G'TGCA_C
Cuts at: 5240 6396 5240
Size: 1156 6027
ApoI r'AATT_y
Cuts at: 1493 2706 2756 2989 3343 3593 4492 4718 4870
Size: 1213 50 233 354 250 899 226 152
Cuts at: 4870 6979 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1213 899 354 250 233 226
152 50
AseI AT'TA_AT
Cuts at: 160 1032 5737 160
Size: 872 4705 1606
Fragments arranged by size:
4705 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 4508 1870
Size: 73 127 2438 4545
Fragments arranged by size:
4545 2438 127 73
BamHI G'GATC_C
Cuts at: 4513 4880 4513
Size: 367 6816
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 4504 5959 618
Size: 325 217 627 225 2492 1455 1842
Fragments arranged by size:
2492 1842 1455 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 4502 1391
Size: 3111 4072
BbeI G_GCGC'C
Cuts at: 2016 2016
Size: 7183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3636 3909 928
Size: 971 1737 273 4202
Fragments arranged by size:
4202 1737 971 273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5374 5408 1903
Size: 34 3437 34 3678
Fragments arranged by size:
3678 3437 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5075 6285 5075
Size: 1210 5973
BclI T'GATC_A
Cuts at: 1548 2857 1548
Size: 1309 5874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5794 136
Size: 107 122 71 631 605 4122 1525
Fragments arranged by size:
4122 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2084 7178 2084
Size: 5094 2089
BlpI GC'TnA_GC
Cuts at: 4600 4600
Size: 7183
Bme1580I G_kGCm'C
Cuts at: 1790 5244 6400 1790
Size: 3454 1156 2573
Fragments arranged by size:
3454 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4914 5872
Size: 416 53 83 399 24 2129 1368 958
Cuts at: 5872 442
Size: 1753
Fragments arranged by size:
2129 1753 1368 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3237 5843 1239
Size: 1998 2606 2579
Fragments arranged by size:
2606 2579 1998
Bpu10I CC'TnA_GC
Cuts at: 3537 3537
Size: 7183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2946 3299 5308 6173 6471 2946
Size: 353 2009 865 298 3658
Fragments arranged by size:
3658 2009 865 353 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5846 882
Size: 617 4347 2219
Fragments arranged by size:
4347 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2602 4974 493
Size: 711 1398 2372 2702
Fragments arranged by size:
2702 2372 1398 711
BsaBI GATnn'nnATC
Cuts at: 4279 7177 4279
Size: 2898 4285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 4990 5372 6007
Size: 53 83 186 936 480 2977 382 635
Cuts at: 6007 275
Size: 1451
Fragments arranged by size:
2977 1451 936 635 480 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3091 5616 6014 6288 6435 6846 3091
Size: 2525 398 274 147 411 3428
Fragments arranged by size:
3428 2525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6958
Fragments arranged by size:
6958 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7183
BseYI C'CCAG_C
Cuts at: 3208 4351 6386 6882 3208
Size: 1143 2035 496 3509
Fragments arranged by size:
3509 2035 1143 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2569 3393 1163
Size: 573 833 824 4953
Fragments arranged by size:
4953 833 824 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4633 5394 6422 1540
Size: 275 186 2632 761 1028 2301
Fragments arranged by size:
2632 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5244 5329 6400 5244
Size: 85 1071 6027
Fragments arranged by size:
6027 1071 85
BsmI GAATG_Cn'
Cuts at: 4153 4694 4787 4153
Size: 541 93 6549
Fragments arranged by size:
6549 541 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4502 5244 5329 6400 1391
Size: 399 2712 742 85 1071 2174
Fragments arranged by size:
2712 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6846 6846
Size: 7183
BspHI T'CATG_A
Cuts at: 2878 5072 6802 2878
Size: 2194 1730 3259
Fragments arranged by size:
3259 2194 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 4538 844
Size: 1822 1872 3489
Fragments arranged by size:
3489 1872 1822
BsrBI CCG'CTC
Cuts at: 1394 1823 2823 5070 1394
Size: 429 1000 2247 3507
Fragments arranged by size:
3507 2247 1000 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3357 5678 5852 65
Size: 1183 2109 2321 174 1396
Fragments arranged by size:
2321 2109 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 3091 5827 6014 1700
Size: 110 205 1076 2736 187 2869
Fragments arranged by size:
2869 2736 1076 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7183
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7183
BssSI C'ACGA_G
Cuts at: 5243 6255 5243
Size: 1012 6171
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7183
BstBI TT'CG_AA
Cuts at: 3391 5987 3391
Size: 2596 4587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7183
BstYI r'GATC_y
Cuts at: 1972 2084 4513 4880 5265 5282 6734 6820 6832
Size: 112 2429 367 385 17 1452 86 12
Cuts at: 6832 7178 1972
Size: 346 1977
Fragments arranged by size:
2429 1977 1452 385 367 346 112 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4110 4110
Size: 7183
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5692
Fragments arranged by size:
5692 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6444
Fragments arranged by size:
6444 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 3855 4699 5493 5513 2581
Size: 1274 844 794 20 4251
Fragments arranged by size:
4251 1274 844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2920 2955 529
Size: 35 2356 35 4757
Fragments arranged by size:
4757 2356 35 35
DraI TTT'AAA
Cuts at: 4487 4487
Size: 7183
DraIII CAC_nnn'GTG
Cuts at: 2398 2398
Size: 7183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6190 809
Size: 507 4874 1802
Fragments arranged by size:
4874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4630 5519 7148
Size: 54 1425 50 275 18 2800 889 1629
Cuts at: 7148 8
Size: 43
Fragments arranged by size:
2800 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4630 1537
Size: 275 2818 4090
Fragments arranged by size:
4090 2818 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5113 1353
Size: 3760 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5770 6144 6290 387
Size: 1064 138 4181 374 146 1280
Fragments arranged by size:
4181 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 3087 3237 3648 5246 5843 6630
Size: 393 495 960 150 411 1598 597 787
Cuts at: 6630 6898 1239
Size: 268 1524
Fragments arranged by size:
1598 1524 960 787 597 495 411 393
268 150
EcoNI CCTnn'n_nnAGG
Cuts at: 4422 4442 4422
Size: 20 7163
EcoRI G'AATT_C
Cuts at: 2989 3593 4492 4870 2989
Size: 604 899 378 5302
Fragments arranged by size:
5302 899 604 378
FspI TGC'GCA
Cuts at: 5689 5689
Size: 7183
HaeII r_GCGC'y
Cuts at: 1678 2016 6330 1678
Size: 338 4314 2531
Fragments arranged by size:
4314 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2159 2178 2191 2857
Size: 32 135 32 574 13 19 13 666
Cuts at: 2857 2889 3210 3242 3475 3487 3507 3519 3676
Size: 32 321 32 233 12 20 12 157
Cuts at: 3676 3708 3728 3760 3910 3942 5824 5856 5898
Size: 32 20 32 150 32 1882 32 42
Cuts at: 5898 5930 1373
Size: 32 2626
Fragments arranged by size:
2626 1882 666 574 321 233 157 150
135 42 32 32 32 32 32 32
32 32 32 20 20 19 13 13
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2738 4480 4527 4773 669
Size: 988 281 800 1742 47 246 3079
Fragments arranged by size:
3079 1742 988 800 281 246 47
HindIII A'AGCT_T
Cuts at: 748 2500 748
Size: 1752 5431
HpaI GTT'AAC
Cuts at: 4480 4773 4480
Size: 293 6890
KasI G'GCGC_C
Cuts at: 2012 2012
Size: 7183
KpnI G_GTAC'C
Cuts at: 4508 4508
Size: 7183
MfeI C'AATT_G
Cuts at: 4782 4782
Size: 7183
MluI A'CGCG_T
Cuts at: 7059 7059
Size: 7183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3582 3977 6297 6481 1305
Size: 2277 395 2320 184 2007
Fragments arranged by size:
2320 2277 2007 395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5361
Fragments arranged by size:
5361 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2418 2457 2604 5141 5500 5659 518
Size: 648 1252 39 147 2537 359 159 2042
Fragments arranged by size:
2537 2042 1252 648 359 159 147 39
MspA1I CmG'CkG
Cuts at: 1606 3215 3371 4387 4599 5276 6424 6669 1606
Size: 1609 156 1016 212 677 1148 245 2120
Fragments arranged by size:
2120 1609 1148 1016 677 245 212 156
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 7183
NarI GG'CG_CC
Cuts at: 2013 2013
Size: 7183
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5692
Fragments arranged by size:
5692 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7183
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 7183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7183
NotI GC'GGCC_GC
Cuts at: 4630 4630
Size: 7183
NspI r_CATG'y
Cuts at: 1132 1229 4344 4541 6086 1132
Size: 97 3115 197 1545 2229
Fragments arranged by size:
3115 2229 1545 197 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7183
PciI A'CATG_T
Cuts at: 6082 6082
Size: 7183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3215 1108
Size: 2107 5076
PmeI GTTT'AAAC
Cuts at: 4487 4487
Size: 7183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5284 6765 6797 5252
Size: 32 1481 32 5638
Fragments arranged by size:
5638 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7183
PsiI TTA'TAA
Cuts at: 4753 4753
Size: 7183
PstI C_TGCA'G
Cuts at: 830 4446 4535 830
Size: 3616 89 3478
Fragments arranged by size:
3616 3478 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7183
PvuII CAG'CTG
Cuts at: 4387 4387
Size: 7183
SalI G'TCGA_C
Cuts at: 1936 2736 4525 1936
Size: 800 1789 4594
Fragments arranged by size:
4594 1789 800
SbfI CC_TGCA'GG
Cuts at: 4535 4535
Size: 7183
ScaI AGT'ACT
Cuts at: 5431 7143 5431
Size: 1712 5471
SfcI C'TryA_G
Cuts at: 826 1045 4101 4442 4531 5666 6347 6538 826
Size: 219 3056 341 89 1135 681 191 1471
Fragments arranged by size:
3056 1471 1135 681 341 219 191 89
SfoI GGC'GCC
Cuts at: 2014 2014
Size: 7183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
SgrAI Cr'CCGG_yG
Cuts at: 1700 2015 1700
Size: 315 6868
SmaI CCC'GGG
Cuts at: 1872 4510 1872
Size: 2638 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 2961 3173 3278 5287 6188
Size: 197 926 127 891 212 105 2009 901
Cuts at: 6188 6450 820
Size: 262 1553
Fragments arranged by size:
2009 1553 926 901 891 262 212 197
127 105
SnaBI TAC'GTA
Cuts at: 493 4974 493
Size: 4481 2702
SpeI A'CTAG_T
Cuts at: 152 3959 152
Size: 3807 3376
SphI G_CATG'C
Cuts at: 1132 4541 1132
Size: 3409 3774
SspI AAT'ATT
Cuts at: 5 52 2701 2837 3560 3915 5107 5
Size: 47 2649 136 723 355 1192 2081
Fragments arranged by size:
2649 2081 1192 723 355 136 47
StuI AGG'CCT
Cuts at: 1517 2174 1517
Size: 657 6526
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 4622 513
Size: 552 939 285 2333 3074
Fragments arranged by size:
3074 2333 939 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2706 3334 5210 5227 5380 5565 6010
Size: 1255 1126 628 1876 17 153 185 445
Cuts at: 6010 325
Size: 1498
Fragments arranged by size:
1876 1498 1255 1126 628 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 5429 7141
Size: 274 80 33 51 1443 939 2513 1712
Cuts at: 7141 96
Size: 138
Fragments arranged by size:
2513 1712 1443 939 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2382 2414 7143 7175 2382
Size: 32 4729 32 2390
Fragments arranged by size:
4729 2390 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7183
XbaI T'CTAG_A
Cuts at: 4519 4519
Size: 7183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2630 2630
Size: 7183
XhoI C'TCGA_G
Cuts at: 1943 2070 1943
Size: 127 7056
XmaI C'CCGG_G
Cuts at: 1870 4508 1870
Size: 2638 4545
XmnI GAAnn'nnTTC
Cuts at: 2682 5312 2682
Size: 2630 4553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4991 276
Size: 53 83 186 936 3457 2468
Fragments arranged by size:
3457 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI