Restriction map for
pFN21AB5642
(Circular) (Six-base) MAPSORT of: pFN21AB5642.seq Check: 6717 from: 1 to: 5755
pFN21AB5642 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3002 3002
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 3565 278
Size: 53 83 186 936 735 1294 2468
Fragments arranged by size:
2468 1294 936 735 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 2556 3076 2556
Size: 520 5235
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5202 5470 1632
Size: 2186 1384 268 1917
Fragments arranged by size:
2186 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2539 3498 5070 2539
Size: 959 1572 3224
Fragments arranged by size:
3224 1572 959
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2405 2745 3080 1870
Size: 73 152 310 340 335 4545
Fragments arranged by size:
4545 340 335 310 152 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2252 2320 2556 2579
Size: 325 217 627 297 168 68 236 23
Cuts at: 2579 2816 3076 4531 618
Size: 237 260 1455 1842
Fragments arranged by size:
1842 1455 627 325 297 260 237 236
217 168 68 23
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbeI G_GCGC'C
Cuts at: 2088 2256 2324 2583 2088
Size: 168 68 259 5260
Fragments arranged by size:
5260 259 168 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2730 2846 928
Size: 971 831 116 3837
Fragments arranged by size:
3837 971 831 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2825 4366 136
Size: 107 122 71 631 605 1153 1541 1525
Fragments arranged by size:
1541 1525 1153 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 3486 4444
Size: 416 53 83 399 24 1377 692 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1377 958 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2561 2654 3042 4415 1239
Size: 1217 105 93 388 1373 2579
Fragments arranged by size:
2579 1373 1217 388 105 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 3880 4745 5043 2348
Size: 1532 865 298 3060
Fragments arranged by size:
3060 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 4418 882
Size: 617 1131 1788 2219
Fragments arranged by size:
2219 1788 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2253 2268 2321
Size: 53 83 186 936 552 168 15 53
Cuts at: 2321 2355 2492 2580 3562 3944 4579 275
Size: 34 137 88 982 382 635 1451
Fragments arranged by size:
1451 982 936 635 552 382 186 168
137 88 83 53 53 34 15
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4396
Fragments arranged by size:
4396 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2390 2522 2525 2528 1186
Size: 1159 45 132 3 3 4413
Fragments arranged by size:
4413 1159 132 45 3 3
BseYI C'CCAG_C
Cuts at: 2937 4958 5454 2937
Size: 2021 496 3238
Fragments arranged by size:
3238 2021 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 1163
Size: 573 948 4234
Fragments arranged by size:
4234 948 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2240 2349 2417 3205 3966 4994
Size: 275 186 239 109 68 788 761 1028
Cuts at: 4994 1540
Size: 2301
Fragments arranged by size:
2301 1028 788 761 275 239 186 109
68
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3074 3816 3901 4972 1391
Size: 399 1284 742 85 1071 2174
Fragments arranged by size:
2174 1284 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2595 3002 3110 844
Size: 1751 407 108 3489
Fragments arranged by size:
3489 1751 407 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2072 2162 2186 2416 2529 2576
Size: 110 250 12 90 24 230 113 47
Cuts at: 2576 4399 4586 1700
Size: 1823 187 2869
Fragments arranged by size:
2869 1823 250 230 187 113 110 90
47 24 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 2675 3815 4827 2675
Size: 1140 1012 3603
Fragments arranged by size:
3603 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3017 794
Size: 2223 3532
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 2346 2414
Size: 54 1425 50 275 18 407 109 68
Cuts at: 2414 3202 4091 5720 8
Size: 788 889 1629 43
Fragments arranged by size:
1629 1425 889 788 407 275 109 68
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2237 2346 2414 3202 1537
Size: 275 425 109 68 788 4090
Fragments arranged by size:
4090 788 425 275 109 68
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 3685 1353
Size: 1014 1318 3423
Fragments arranged by size:
3423 1318 1014
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2193 4342 4716 4862 387
Size: 1064 138 604 2149 374 146 1280
Fragments arranged by size:
2149 1280 1064 604 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2561 2654 3042 3818 4415 5202
Size: 393 824 105 93 388 776 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 776 597 393 388 268
105 93
EcoO109I rG'GnC_Cy
Cuts at: 2260 2971 2260
Size: 711 5044
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2714 2714
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2088 2187 2256 2324 2583 2633 4902 1678
Size: 410 99 69 68 259 50 2269 2531
Fragments arranged by size:
2531 2269 410 259 99 69 68 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2536 2568 2720
Size: 32 135 32 456 32 476 32 152
Cuts at: 2720 2739 2752 2771 2785 2817 4396 4428 4470
Size: 19 13 19 14 32 1579 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1579 476 456 152 135 42 32
32 32 32 32 32 32 19 19
14 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2084 2252 2320 2579 2084
Size: 168 68 259 5260
Fragments arranged by size:
5260 259 168 68
KpnI G_GTAC'C
Cuts at: 2560 3080 2560
Size: 520 5235
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2351 2431 3171 3848 4996 5241 1606
Size: 473 272 80 740 677 1148 245 2120
Fragments arranged by size:
2120 1148 740 677 473 272 245 80
NaeI GCC'GGC
Cuts at: 2062 2074 2164 2188 2418 2062
Size: 12 90 24 230 5399
Fragments arranged by size:
5399 230 90 24 12
NarI GG'CG_CC
Cuts at: 2085 2253 2321 2580 2085
Size: 168 68 259 5260
Fragments arranged by size:
5260 259 168 68
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2060 2072 2162 2186 2416 2060
Size: 12 90 24 230 5399
Fragments arranged by size:
5399 230 90 24 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 2237 3202 2237
Size: 965 4790
NspI r_CATG'y
Cuts at: 1132 1229 2698 3113 4658 1132
Size: 97 1469 415 1545 2229
Fragments arranged by size:
2229 1545 1469 415 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PfoI T'CCnGG_A
Cuts at: 2222 2222
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2971 2260
Size: 711 5044
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2086 2254 2322 2581 2086
Size: 168 68 259 5260
Fragments arranged by size:
5260 259 168 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 2186 2576 1700
Size: 486 390 4879
Fragments arranged by size:
4879 486 390
SmaI CCC'GGG
Cuts at: 1872 2097 3082 1872
Size: 225 985 4545
Fragments arranged by size:
4545 985 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2745 3859 4760 5022 820
Size: 197 926 384 418 1114 901 262 1553
Fragments arranged by size:
1553 1114 926 901 418 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2698 3113 1132
Size: 1566 415 3774
Fragments arranged by size:
3774 1566 415
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 1517
Size: 612 5143
StyI C'CwwG_G
Cuts at: 513 1065 2583 2689 3194 513
Size: 552 1518 106 505 3074
Fragments arranged by size:
3074 1518 552 505 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 2745 1943
Size: 802 4953
XmaI C'CCGG_G
Cuts at: 1870 2095 3080 1870
Size: 225 985 4545
Fragments arranged by size:
4545 985 225
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 3563 276
Size: 53 83 186 936 735 1294 2468
Fragments arranged by size:
2468 1294 936 735 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI