Restriction map for
pFN21AB5635
(Circular) (Six-base) MAPSORT of: pFN21AB5635.seq Check: 4115 from: 1 to: 6127
pFN21AB5635 6127 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 5574 5842 1632
Size: 2558 1384 268 1917
Fragments arranged by size:
2558 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 3870 5442 3870
Size: 1572 4555
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 3448 4903 618
Size: 325 217 627 481 1180 1455 1842
Fragments arranged by size:
1842 1455 1180 627 481 325 217
BanII G_rGCy'C
Cuts at: 1391 3446 1391
Size: 2055 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2692 928
Size: 971 793 4363
Fragments arranged by size:
4363 971 793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2743 2777 4318 4352 1903
Size: 34 806 34 1541 34 3678
Fragments arranged by size:
3678 1541 806 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3099 4019 5229 3099
Size: 920 1210 3997
Fragments arranged by size:
3997 1210 920
BclI T'GATC_A
Cuts at: 1548 3270 1548
Size: 1722 4405
BfrBI ATG'CAT
Cuts at: 2521 2521
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2273 4188 5344 1790
Size: 483 1915 1156 2573
Fragments arranged by size:
2573 1915 1156 483
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2718 3858 4816
Size: 416 53 83 399 24 1301 1140 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1753 1301 1140 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3140 4787 1239
Size: 1901 1647 2579
Fragments arranged by size:
2579 1901 1647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3194 4252 5117 5415 3194
Size: 1058 865 298 3906
Fragments arranged by size:
3906 1058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 3275 6121 3275
Size: 2846 3281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2553 4560 4958 5232 5379 5790 2553
Size: 2007 398 274 147 411 2890
Fragments arranged by size:
2890 2007 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2184 1186
Size: 998 5129
BseYI C'CCAG_C
Cuts at: 5330 5826 5330
Size: 496 5631
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 2296 2469 2557 1163
Size: 573 441 119 173 88 4733
Fragments arranged by size:
4733 573 441 173 119 88
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4188 4273 5344 4188
Size: 85 1071 4971
Fragments arranged by size:
4971 1071 85
BsiWI C'GTAC_G
Cuts at: 2904 2904
Size: 6127
BsmI GAATG_Cn'
Cuts at: 2521 3154 3638 3731 2521
Size: 633 484 93 4917
Fragments arranged by size:
4917 633 484 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 3446 4188 4273 5344 1391
Size: 399 483 1173 742 85 1071 2174
Fragments arranged by size:
2174 1173 1071 742 483 399 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 2782 3019 4016 5746 2782
Size: 237 997 1730 3163
Fragments arranged by size:
3163 1730 997 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2731 4014 1394
Size: 429 908 1283 3507
Fragments arranged by size:
3507 1283 908 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2440 2701 2806 4622 4796 65
Size: 1183 1041 151 261 105 1816 174 1396
Fragments arranged by size:
1816 1396 1183 1041 261 174 151 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 2094 4187 5199 2094
Size: 2093 1012 3022
Fragments arranged by size:
3022 2093 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 3138 4931 3138
Size: 1793 4334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 2683
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2331 3205 3457 3824 4209 4226 5678 5764
Size: 359 874 252 367 385 17 1452 86
Cuts at: 5764 5776 6122 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 874 385 367 359 346 252
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3246 3643 4437 4457 3246
Size: 397 794 20 4916
Fragments arranged by size:
4916 794 397 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3339 3431 3339
Size: 92 6035
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 5134 809
Size: 507 999 2819 1802
Fragments arranged by size:
2819 1802 999 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2236 2685 3300
Size: 54 1425 50 275 18 406 449 615
Cuts at: 3300 3574 4463 6092 8
Size: 274 889 1629 43
Fragments arranged by size:
1629 1425 889 615 449 406 275 274
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 3123 4057 1353
Size: 1431 339 934 3423
Fragments arranged by size:
3423 1431 934 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3140 4190 4787 5574 5842 1239
Size: 393 1508 1050 597 787 268 1524
Fragments arranged by size:
1524 1508 1050 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2946 2946
Size: 6127
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2391 2423 3335 3367 4768
Size: 32 135 32 819 32 912 32 1401
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 912 819 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3471 3717 669
Size: 988 281 1048 485 246 3079
Fragments arranged by size:
3079 1048 988 485 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 2986 3717 2986
Size: 731 5396
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 2772 3726 2772
Size: 954 5173
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2447 3306 5241 5425 1305
Size: 1142 859 1935 184 2007
Fragments arranged by size:
2007 1935 1142 859 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 10
Size: 54 1425 343 406 3899
Fragments arranged by size:
3899 1425 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 4085 4444 4603 518
Size: 648 1152 1767 359 159 2042
Fragments arranged by size:
2042 1767 1152 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2298 2737 3101 3543 4220 5368 5613 1606
Size: 692 439 364 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 442 439 364 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5575
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2523 2523
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2042 2965 3485 5030 1132
Size: 97 813 923 520 1545 2229
Fragments arranged by size:
2229 1545 923 813 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 1108
Size: 1234 4893
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 2504 3697 2504
Size: 1193 4934
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2737 2737
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SapI GCTCTTCn'nnn_
Cuts at: 2784 2784
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2226 3475 4610 5291 5482 826
Size: 219 1181 1249 1135 681 191 1471
Fragments arranged by size:
1471 1249 1181 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3209 4231 5132 5394 820
Size: 197 926 1266 1022 901 262 1553
Fragments arranged by size:
1553 1266 1022 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 3566 513
Size: 552 2501 3074
Fragments arranged by size:
3074 2501 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4373 6085 96
Size: 274 80 33 51 1443 2396 1712 138
Fragments arranged by size:
2396 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI