Restriction map for
pFN21AB5634
(Circular) (Six-base) MAPSORT of: pFN21AB5634.seq Check: 7874 from: 1 to: 7057
pFN21AB5634 7057 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2072 4054 2072
Size: 1982 5075
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4867 278
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3315 4400 1937
Size: 1378 1085 4594
Fragments arranged by size:
4594 1378 1085
Acc65I G'GTAC_C
Cuts at: 4378 4378
Size: 7057
AclI AA'CG_TT
Cuts at: 1563 5184 5557 1563
Size: 3621 373 3063
Fragments arranged by size:
3621 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 2829 3167 5120 6504 6772 1632
Size: 974 223 338 1953 1384 268 1917
Fragments arranged by size:
1953 1917 1384 974 338 268 223
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6860
AflIII A'CryG_T
Cuts at: 1203 2307 5956 6933 1203
Size: 1104 3649 977 1327
Fragments arranged by size:
3649 1327 1104 977
AgeI A'CCGG_T
Cuts at: 5888 5888
Size: 7057
AhdI GACnn_n'nnGTC
Cuts at: 5786 5786
Size: 7057
AleI CACnn'nnGTG
Cuts at: 1166 2819 2831 3098 1166
Size: 1653 12 267 5125
Fragments arranged by size:
5125 1653 267 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3923 1184
Size: 2739 4318
AlwNI CAG_nnn'CTG
Cuts at: 4800 6372 4800
Size: 1572 5485
ApaI G_GGCC'C
Cuts at: 4110 4110
Size: 7057
ApaLI G'TGCA_C
Cuts at: 5114 6270 5114
Size: 1156 5901
ApoI r'AATT_y
Cuts at: 1493 3548 4366 4592 4744 6853 1493
Size: 2055 818 226 152 2109 1697
Fragments arranged by size:
2109 2055 1697 818 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5611 160
Size: 872 4579 1606
Fragments arranged by size:
4579 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
AvaI C'yCGr_G
Cuts at: 1870 1943 2719 4109 4338 4382 1870
Size: 73 776 1390 229 44 4545
Fragments arranged by size:
4545 1390 776 229 73 44
BamHI G'GATC_C
Cuts at: 4387 4754 4387
Size: 367 6690
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2133 2825 3704 4244
Size: 325 217 627 252 94 692 879 540
Cuts at: 4244 4378 5833 618
Size: 134 1455 1842
Fragments arranged by size:
1842 1455 879 692 627 540 325 252
217 134 94
BanII G_rGCy'C
Cuts at: 1391 2840 2868 3056 3076 3111 4110 4211 4376
Size: 1449 28 188 20 35 999 101 165
Cuts at: 4376 1391
Size: 4072
Fragments arranged by size:
4072 1449 999 188 165 101 35 28
20
BbeI G_GCGC'C
Cuts at: 2043 2137 2043
Size: 94 6963
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 2817 928
Size: 971 373 545 5168
Fragments arranged by size:
5168 971 545 373
BbvCI CC'TCA_GC
Cuts at: 4232 4232
Size: 7057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5248 5282 1903
Size: 34 50 34 3227 34 3678
Fragments arranged by size:
3678 3227 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4949 6159 3215
Size: 1734 1210 4113
Fragments arranged by size:
4113 1734 1210
BclI T'GATC_A
Cuts at: 1548 3843 1548
Size: 2295 4762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4027 4081 5668
Size: 107 122 71 631 605 2355 54 1587
Cuts at: 5668 136
Size: 1525
Fragments arranged by size:
2355 1587 1525 631 605 122 107 71
54
BglII A'GATC_T
Cuts at: 3501 7052 3501
Size: 3551 3506
BlpI GC'TnA_GC
Cuts at: 4317 4474 4317
Size: 157 6900
Bme1580I G_kGCm'C
Cuts at: 1790 2726 2828 3707 4099 4110 4247 5118 6274
Size: 936 102 879 392 11 137 871 1156
Cuts at: 6274 1790
Size: 2573
Fragments arranged by size:
2573 1156 936 879 871 392 137 102
11
BmgBI CAC'GTC
Cuts at: 1318 2308 2434 4267 1318
Size: 990 126 1833 4108
Fragments arranged by size:
4108 1833 990 126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4300 4788 5746
Size: 416 53 83 399 24 2883 488 958
Cuts at: 5746 442
Size: 1753
Fragments arranged by size:
2883 1753 958 488 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 7057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 5717 1239
Size: 1178 3300 2579
Fragments arranged by size:
3300 2579 1178
Bpu10I CC'TnA_GC
Cuts at: 4232 4232
Size: 7057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5182 6047 6345 5182
Size: 865 298 5894
Fragments arranged by size:
5894 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2550 5720 882
Size: 617 1051 3170 2219
Fragments arranged by size:
3170 2219 1051 617
BsaAI yAC'GTr
Cuts at: 493 1204 4848 493
Size: 711 3644 2702
Fragments arranged by size:
3644 2702 711
BsaBI GATnn'nnATC
Cuts at: 7051 7051
Size: 7057
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2134 4041 4864
Size: 53 83 186 936 507 94 1907 823
Cuts at: 4864 5246 5881 275
Size: 382 635 1451
Fragments arranged by size:
1907 1451 936 823 635 507 382 186
94 83 53
BsaWI w'CCGG_w
Cuts at: 2874 3876 5490 5888 6162 6309 6720 2874
Size: 1002 1614 398 274 147 411 3211
Fragments arranged by size:
3211 1614 1002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2253 2283 4193 4223 4337
Size: 30 165 30 850 30 1910 30 114
Cuts at: 4337 4367 1178
Size: 30 3868
Fragments arranged by size:
3868 1910 850 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 3959 4358 1186
Size: 829 1944 399 3885
Fragments arranged by size:
3885 1944 829 399
BseYI C'CCAG_C
Cuts at: 6260 6756 6260
Size: 496 6561
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4088 4172 1163
Size: 573 2352 84 4048
Fragments arranged by size:
4048 2352 573 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4507 5268 6296 1540
Size: 275 186 2506 761 1028 2301
Fragments arranged by size:
2506 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3993 5118 5203 6274 3993
Size: 1125 85 1071 4776
Fragments arranged by size:
4776 1125 1071 85
BsmI GAATG_Cn'
Cuts at: 2245 4568 4661 2245
Size: 2323 93 4641
Fragments arranged by size:
4641 2323 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2298 2298
Size: 7057
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2726 2828 2840 2868 3056 3076 3111
Size: 399 936 102 12 28 188 20 35
Cuts at: 3111 3707 3993 4099 4110 4211 4247 4376 5118
Size: 596 286 106 11 101 36 129 742
Cuts at: 5118 5203 6274 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 936 742 596 399 286 188
129 106 102 101 85 36 35 28
20 12 11
BspEI T'CCGG_A
Cuts at: 3876 6720 3876
Size: 2844 4213
BspHI T'CATG_A
Cuts at: 2016 2428 4946 6676 2016
Size: 412 2518 1730 2397
Fragments arranged by size:
2518 2397 1730 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2259 4054 4412 844
Size: 1228 187 1795 358 3489
Fragments arranged by size:
3489 1795 1228 358 187
BsrBI CCG'CTC
Cuts at: 1394 1823 4944 1394
Size: 429 3121 3507
Fragments arranged by size:
3507 3121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5552 5726 65
Size: 1183 4304 174 1396
Fragments arranged by size:
4304 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 5701 5888 1700
Size: 110 320 3571 187 2869
Fragments arranged by size:
3571 2869 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7057
BssHII G'CGCG_C
Cuts at: 1925 3891 1925
Size: 1966 5091
BssSI C'ACGA_G
Cuts at: 3920 5117 6129 3920
Size: 1197 1012 4848
Fragments arranged by size:
4848 1197 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2084 2534 794
Size: 1290 450 5317
Fragments arranged by size:
5317 1290 450
BstBI TT'CG_AA
Cuts at: 5861 5861
Size: 7057
BstEII G'GTnAC_C
Cuts at: 1184 3931 1184
Size: 2747 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 2737 3352 2737
Size: 615 6442
BstYI r'GATC_y
Cuts at: 1972 3501 3915 4387 4754 5139 5156 6608 6694
Size: 1529 414 472 367 385 17 1452 86
Cuts at: 6694 6706 7052 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1529 1452 472 414 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3060 3189 3481 3666 3720 4006 4025
Size: 552 1995 129 292 185 54 286 19
Cuts at: 4025 513
Size: 3545
Fragments arranged by size:
3545 1995 552 292 286 185 129 54
19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6318
Fragments arranged by size:
6318 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2076 2358 2535 3810 4573 5367 5387 2067
Size: 9 282 177 1275 763 794 20 3737
Fragments arranged by size:
3737 1275 794 763 282 177 20 9
ClaI AT'CG_AT
Cuts at: 1554 2610 1554
Size: 1056 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7022
DraI TTT'AAA
Cuts at: 4361 4361
Size: 7057
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3311 6064 809
Size: 507 1995 2753 1802
Fragments arranged by size:
2753 1995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2727 3186 3484
Size: 54 1425 50 275 18 897 459 298
Cuts at: 3484 3603 3723 3774 4504 5393 7022 8
Size: 119 120 51 730 889 1629 43
Fragments arranged by size:
1629 1425 897 889 730 459 298 275
120 119 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4504 1537
Size: 275 2692 4090
Fragments arranged by size:
4090 2692 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 4987 1353
Size: 819 2815 3423
Fragments arranged by size:
3423 2815 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4166 5644 6018 6164 387
Size: 1064 138 2577 1478 374 146 1280
Fragments arranged by size:
2577 1478 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2606 2829 3167 5120 5717 6504
Size: 393 785 189 223 338 1953 597 787
Cuts at: 6504 6772 1239
Size: 268 1524
Fragments arranged by size:
1953 1524 787 785 597 393 338 268
223 189
EcoO109I rG'GnC_Cy
Cuts at: 2505 2704 2854 4106 4295 2505
Size: 199 150 1252 189 5267
Fragments arranged by size:
5267 1252 199 189 150
EcoRI G'AATT_C
Cuts at: 4366 4744 4366
Size: 378 6679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3673 3673
Size: 7057
FspI TGC'GCA
Cuts at: 4062 5563 4062
Size: 1501 5556
HaeII r_GCGC'y
Cuts at: 1678 2043 2137 6204 1678
Size: 365 94 4067 2531
Fragments arranged by size:
4067 2531 365 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 2613 2645 3063
Size: 32 135 32 977 32 32 32 418
Cuts at: 3063 3095 3213 3245 3679 3711 5698 5730 5772
Size: 32 118 32 434 32 1987 32 42
Cuts at: 5772 5804 1373
Size: 32 2626
Fragments arranged by size:
2626 1987 977 434 418 135 118 42
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4401 4647 669
Size: 988 281 2463 246 3079
Fragments arranged by size:
3079 2463 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2644 748
Size: 1896 5161
HpaI GTT'AAC
Cuts at: 4647 4647
Size: 7057
KasI G'GCGC_C
Cuts at: 2039 2133 2039
Size: 94 6963
KpnI G_GTAC'C
Cuts at: 4382 4382
Size: 7057
MfeI C'AATT_G
Cuts at: 4656 4656
Size: 7057
MluI A'CGCG_T
Cuts at: 6933 6933
Size: 7057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4281 6171 6355 1305
Size: 2976 1890 184 2007
Fragments arranged by size:
2976 2007 1890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3188 3486 10
Size: 54 1425 343 1356 298 3581
Fragments arranged by size:
3581 1425 1356 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2519 2819 2831 3098 3839 5015
Size: 648 1006 347 300 12 267 741 1176
Cuts at: 5015 5374 5533 518
Size: 359 159 2042
Fragments arranged by size:
2042 1176 1006 741 648 359 347 300
267 159 12
MspA1I CmG'CkG
Cuts at: 1606 3062 3145 3490 3928 4008 4027 4473 5150
Size: 1456 83 345 438 80 19 446 677
Cuts at: 5150 6298 6543 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1456 1148 677 446 438 345 245
83 80 19
NarI GG'CG_CC
Cuts at: 2040 2134 2040
Size: 94 6963
NcoI C'CATG_G
Cuts at: 513 1065 3189 3481 513
Size: 552 2124 292 4089
Fragments arranged by size:
4089 2124 552 292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7057
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7057
NotI GC'GGCC_GC
Cuts at: 4504 4504
Size: 7057
NspI r_CATG'y
Cuts at: 1132 1229 4415 5960 1132
Size: 97 3186 1545 2229
Fragments arranged by size:
3186 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2701 4240 1348
Size: 1353 1539 4165
Fragments arranged by size:
4165 1539 1353
PciI A'CATG_T
Cuts at: 5956 5956
Size: 7057
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3805 1108
Size: 2697 4360
PmeI GTTT'AAAC
Cuts at: 4361 4361
Size: 7057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5126 5158 6639 6671 5126
Size: 32 1481 32 5512
Fragments arranged by size:
5512 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2505 2854 4295 2505
Size: 349 1441 5267
Fragments arranged by size:
5267 1441 349
PshAI GACnn'nnGTC
Cuts at: 1613 2510 1613
Size: 897 6160
PsiI TTA'TAA
Cuts at: 4627 4627
Size: 7057
PspOMI G'GGCC_C
Cuts at: 4106 4106
Size: 7057
PstI C_TGCA'G
Cuts at: 830 3501 4409 830
Size: 2671 908 3478
Fragments arranged by size:
3478 2671 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7057
PvuII CAG'CTG
Cuts at: 3145 3490 3928 3145
Size: 345 438 6274
Fragments arranged by size:
6274 438 345
SacII CC_GC'GG
Cuts at: 3063 4009 4028 3063
Size: 946 19 6092
Fragments arranged by size:
6092 946 19
SalI G'TCGA_C
Cuts at: 1936 4399 1936
Size: 2463 4594
SanDI GG'GwC_CC
Cuts at: 4295 4295
Size: 7057
SbfI CC_TGCA'GG
Cuts at: 4409 4409
Size: 7057
ScaI AGT'ACT
Cuts at: 5305 7017 5305
Size: 1712 5345
SfcI C'TryA_G
Cuts at: 826 1045 3497 4405 5540 6221 6412 826
Size: 219 2452 908 1135 681 191 1471
Fragments arranged by size:
2452 1471 1135 908 681 219 191
SfoI GGC'GCC
Cuts at: 2041 2135 2041
Size: 94 6963
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
SgrAI Cr'CCGG_yG
Cuts at: 1700 2130 1700
Size: 430 6627
SmaI CCC'GGG
Cuts at: 1872 4111 4340 4384 1872
Size: 2239 229 44 4545
Fragments arranged by size:
4545 2239 229 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5161 6062 6324 820
Size: 197 926 3218 901 262 1553
Fragments arranged by size:
3218 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4848 493
Size: 4355 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7057
SphI G_CATG'C
Cuts at: 1132 4415 1132
Size: 3283 3774
SspI AAT'ATT
Cuts at: 5 52 4981 5
Size: 47 4929 2081
Fragments arranged by size:
4929 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7057
StyI C'CwwG_G
Cuts at: 513 1065 3189 3481 3648 4496 513
Size: 552 2124 292 167 848 3074
Fragments arranged by size:
3074 2124 848 552 292 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2141 2667 2795 5084 5101 5254
Size: 1255 543 18 526 128 2289 17 153
Cuts at: 5254 5439 5884 325
Size: 185 445 1498
Fragments arranged by size:
2289 1498 1255 543 526 445 185 153
128 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2790 2886 5303
Size: 274 80 33 51 1443 813 96 2417
Cuts at: 5303 7015 96
Size: 1712 138
Fragments arranged by size:
2417 1712 1443 813 274 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 2253 2285 7017 7049 2179
Size: 32 42 32 4732 32 2187
Fragments arranged by size:
4732 2187 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7057
XbaI T'CTAG_A
Cuts at: 4393 4393
Size: 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2974 2323
Size: 651 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7057
XmaI C'CCGG_G
Cuts at: 1870 4109 4338 4382 1870
Size: 2239 229 44 4545
Fragments arranged by size:
4545 2239 229 44
XmnI GAAnn'nnTTC
Cuts at: 5186 5186
Size: 7057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4865 276
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SapI SexAI SfiI SrfI SwaI