Restriction map for
pFN21AB5628
(Circular) (Six-base) MAPSORT of: pFN21AB5628.seq Check: 2493 from: 1 to: 8833
pFN21AB5628 8833 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4386 6643 278
Size: 53 83 186 936 2850 2257 2468
Fragments arranged by size:
2850 2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6176 1937
Size: 4239 4594
Acc65I G'GTAC_C
Cuts at: 6119 6154 6119
Size: 35 8798
AclI AA'CG_TT
Cuts at: 1563 6960 7333 1563
Size: 5397 373 3063
Fragments arranged by size:
5397 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 2384 2890 4064 4493 6896 8280 8548
Size: 681 71 506 1174 429 2403 1384 268
Cuts at: 8548 1632
Size: 1917
Fragments arranged by size:
2403 1917 1384 1174 681 506 429 268
71
AfeI AGC'GCT
Cuts at: 3611 3611
Size: 8833
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8636
AflIII A'CryG_T
Cuts at: 1203 3018 4407 7732 8709 1203
Size: 1815 1389 3325 977 1327
Fragments arranged by size:
3325 1815 1389 1327 977
AgeI A'CCGG_T
Cuts at: 5133 7664 5133
Size: 2531 6302
AhdI GACnn_n'nnGTC
Cuts at: 4966 5815 7562 4966
Size: 849 1747 6237
Fragments arranged by size:
6237 1747 849
AleI CACnn'nnGTG
Cuts at: 1166 2841 4590 5177 1166
Size: 1675 1749 587 4822
Fragments arranged by size:
4822 1749 1675 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6086 1184
Size: 4902 3931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 8801
AlwNI CAG_nnn'CTG
Cuts at: 3489 4426 5711 6576 8148 3489
Size: 937 1285 865 1572 4174
Fragments arranged by size:
4174 1572 1285 937 865
ApaI G_GGCC'C
Cuts at: 3211 3429 4303 5898 3211
Size: 218 874 1595 6146
Fragments arranged by size:
6146 1595 874 218
ApaLI G'TGCA_C
Cuts at: 3091 4521 6890 8046 3091
Size: 1430 2369 1156 3878
Fragments arranged by size:
3878 2369 1430 1156
ApoI r'AATT_y
Cuts at: 1493 2439 3125 3893 4211 5386 6142 6368 6520
Size: 946 686 768 318 1175 756 226 152
Cuts at: 6520 8629 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1175 946 768 756 686 318
226 152
AseI AT'TA_AT
Cuts at: 160 1032 2252 5550 7387 160
Size: 872 1220 3298 1837 1606
Fragments arranged by size:
3298 1837 1606 1220 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8833
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2464 3343 4302 4868 5827 5873
Size: 73 139 382 879 959 566 959 46
Cuts at: 5873 6158 1870
Size: 285 4545
Fragments arranged by size:
4545 959 959 879 566 382 285 139
73 46
AvrII C'CTAG_G
Cuts at: 3508 3508
Size: 8833
BamHI G'GATC_C
Cuts at: 5317 5485 6163 6530 5317
Size: 168 678 367 7620
Fragments arranged by size:
7620 678 367 168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2784 2996 3395 3532
Size: 325 217 627 335 662 212 399 137
Cuts at: 3532 3578 4034 4110 4864 5267 5939 6119 6154
Size: 46 456 76 754 403 672 180 35
Cuts at: 6154 7609 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 754 672 662 627 456 403
399 335 325 217 212 180 137 76
46 35
BanII G_rGCy'C
Cuts at: 1391 2159 2493 2738 3211 3344 3429 3455 3557
Size: 768 334 245 473 133 85 26 102
Cuts at: 3557 3890 4303 4814 5256 5834 5898 6152 1391
Size: 333 413 511 442 578 64 254 4072
Fragments arranged by size:
4072 768 578 511 473 442 413 334
333 254 245 133 102 85 64 26
BbeI G_GCGC'C
Cuts at: 2788 3000 4038 4114 5271 2788
Size: 212 1038 76 1157 6350
Fragments arranged by size:
6350 1157 1038 212 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5646 928
Size: 971 3747 4115
Fragments arranged by size:
4115 3747 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7024 7058 1903
Size: 34 5087 34 3678
Fragments arranged by size:
5087 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6725 7935 6725
Size: 1210 7623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8833
BfrBI ATG'CAT
Cuts at: 4497 4497
Size: 8833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3363 4139 7444
Size: 107 122 71 631 605 1691 776 3305
Cuts at: 7444 136
Size: 1525
Fragments arranged by size:
3305 1691 1525 776 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4431 5949 8828 4431
Size: 1518 2879 4436
Fragments arranged by size:
4436 2879 1518
BlpI GC'TnA_GC
Cuts at: 2135 2852 5833 6250 2135
Size: 717 2981 417 4718
Fragments arranged by size:
4718 2981 717 417
Bme1580I G_kGCm'C
Cuts at: 1790 2921 3095 3211 3400 3429 3537 3581 4303
Size: 1131 174 116 189 29 108 44 722
Cuts at: 4303 4525 5898 5942 6894 8050 1790
Size: 222 1373 44 952 1156 2573
Fragments arranged by size:
2573 1373 1156 1131 952 722 222 189
174 116 108 44 44 29
BmgBI CAC'GTC
Cuts at: 1318 3936 1318
Size: 2618 6215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 5905 6564
Size: 416 53 83 399 24 2015 2473 659
Cuts at: 6564 7522 442
Size: 958 1753
Fragments arranged by size:
2473 2015 1753 958 659 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4376 1056
Size: 3320 5513
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5782 5982 5782
Size: 200 8633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2765 2889 4573 5380 5688 5824 6109
Size: 966 560 124 1684 807 308 136 285
Cuts at: 6109 7493 1239
Size: 1384 2579
Fragments arranged by size:
2579 1684 1384 966 807 560 308 285
136 124
Bpu10I CC'TnA_GC
Cuts at: 4070 5630 4070
Size: 1560 7273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4019 5132 6958 7823 8121 3568
Size: 451 1113 1826 865 298 4280
Fragments arranged by size:
4280 1826 1113 865 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2181 7496 882
Size: 617 682 5315 2219
Fragments arranged by size:
5315 2219 682 617
BsaAI yAC'GTr
Cuts at: 493 1204 3019 3628 6013 6624 493
Size: 711 1815 609 2385 611 2702
Fragments arranged by size:
2702 2385 1815 711 611 609
BsaBI GATnn'nnATC
Cuts at: 8827 8827
Size: 8833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 2785 2997 3134
Size: 53 83 186 936 765 487 212 137
Cuts at: 3134 3231 4035 4111 4383 4767 5268 6640 7022
Size: 97 804 76 272 384 501 1372 382
Cuts at: 7022 7657 275
Size: 635 1451
Fragments arranged by size:
1451 1372 936 804 765 635 501 487
384 382 272 212 186 137 97 83
76 53
BsaWI w'CCGG_w
Cuts at: 3436 4970 5133 7266 7664 7938 8085 8496 3436
Size: 1534 163 2133 398 274 147 411 3773
Fragments arranged by size:
3773 2133 1534 411 398 274 163 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 3629 3659 5679
Size: 30 165 30 1293 30 903 30 2020
Cuts at: 5679 5709 1178
Size: 30 4302
Fragments arranged by size:
4302 2020 1293 903 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3002 3005 3314 3565 4138 5324 5582 1186
Size: 1816 3 309 251 573 1186 258 4437
Fragments arranged by size:
4437 1816 1186 573 309 258 251 3
BseYI C'CCAG_C
Cuts at: 2015 3399 3538 4091 4137 4368 4547 4654 5769
Size: 1384 139 553 46 231 179 107 1115
Cuts at: 5769 5888 8036 8532 2015
Size: 119 2148 496 2316
Fragments arranged by size:
2316 2148 1384 1115 553 496 231 179
139 119 107 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2867 4118 4254 5033 5887 1163
Size: 573 1131 1251 136 779 854 4109
Fragments arranged by size:
4109 1251 1131 854 779 573 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 2529 2892 2929 3099 4636
Size: 275 186 304 224 363 37 170 1537
Cuts at: 4636 5007 5755 6283 7044 8072 1540
Size: 371 748 528 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 748 528 371 363
304 275 224 186 170 37
BsiHKAI G_wGCw'C
Cuts at: 2136 2267 2738 2858 3095 3344 3415 3557 3633
Size: 131 471 120 237 249 71 142 76
Cuts at: 3633 4525 4721 4749 6012 6894 6979 8050 2136
Size: 892 196 28 1263 882 85 1071 2919
Fragments arranged by size:
2919 1263 1071 892 882 471 249 237
196 142 131 120 85 76 71 28
BsmI GAATG_Cn'
Cuts at: 3796 3834 4499 4899 6344 6437 3796
Size: 38 665 400 1445 93 6192
Fragments arranged by size:
6192 1445 665 400 93 38
BsmBI CGTCTCn'nnnn_
Cuts at: 5926 5926
Size: 8833
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2159 2267 2493 2738 2858 2921
Size: 399 346 23 108 226 245 120 63
Cuts at: 2921 3095 3211 3344 3400 3415 3429 3455 3537
Size: 174 116 133 56 15 14 26 82
Cuts at: 3537 3557 3581 3633 3890 4303 4525 4721 4749
Size: 20 24 52 257 413 222 196 28
Cuts at: 4749 4814 5256 5834 5898 5942 6012 6152 6894
Size: 65 442 578 64 44 70 140 742
Cuts at: 6894 6979 8050 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 442 413 399 346
257 245 226 222 196 174 140 133
120 116 108 85 82 70 65 64
63 56 52 44 28 26 24 23
20 15 14
BspEI T'CCGG_A
Cuts at: 3436 4970 8496 3436
Size: 1534 3526 3773
Fragments arranged by size:
3773 3526 1534
BspHI T'CATG_A
Cuts at: 2145 2233 2614 2650 4443 6722 8452 2145
Size: 88 381 36 1793 2279 1730 2526
Fragments arranged by size:
2526 2279 1793 1730 381 88 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4531 5091 6188 844
Size: 3687 560 1097 3489
Fragments arranged by size:
3687 3489 1097 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3470 4984 6720 1394
Size: 429 1647 1514 1736 3507
Fragments arranged by size:
3507 1736 1647 1514 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2036 3066 4084 7328 7502 65
Size: 1183 788 1030 1018 3244 174 1396
Fragments arranged by size:
3244 1396 1183 1030 1018 788 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 2928 4107 5133 7477 7664 1700
Size: 110 283 835 1179 1026 2344 187 2869
Fragments arranged by size:
2869 2344 1179 1026 835 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8833
BssSI C'ACGA_G
Cuts at: 2731 4699 6893 7905 2731
Size: 1968 2194 1012 3659
Fragments arranged by size:
3659 2194 1968 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2669 3708 4426 794
Size: 1875 1039 718 5201
Fragments arranged by size:
5201 1875 1039 718
BstBI TT'CG_AA
Cuts at: 2310 3862 7637 2310
Size: 1552 3775 3506
Fragments arranged by size:
3775 3506 1552
BstEII G'GTnAC_C
Cuts at: 1184 2818 2947 1184
Size: 1634 129 7070
Fragments arranged by size:
7070 1634 129
BstYI r'GATC_y
Cuts at: 1972 2510 3054 3738 3959 4431 5116 5317 5485
Size: 538 544 684 221 472 685 201 168
Cuts at: 5485 5949 6163 6530 6915 6932 8384 8470 8482
Size: 464 214 367 385 17 1452 86 12
Cuts at: 8482 8828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 685 684 544 538 472 464
385 367 346 221 214 201 168 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2770 3582 5007 5190 513
Size: 552 1705 812 1425 183 4156
Fragments arranged by size:
4156 1705 1425 812 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2705 487
Size: 683 56 1479 6615
Fragments arranged by size:
6615 1479 683 56
BtsI GCAGTG_nn'
Cuts at: 3650 3716 4891 5789 6349 7143 7163 3650
Size: 66 1175 898 560 794 20 5320
Fragments arranged by size:
5320 1175 898 794 560 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8798
DraI TTT'AAA
Cuts at: 2682 6137 2682
Size: 3455 5378
DraIII CAC_nnn'GTG
Cuts at: 2129 2284 2129
Size: 155 8678
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2646 7840 809
Size: 507 1330 5194 1802
Fragments arranged by size:
5194 1802 1330 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2526 2877
Size: 54 1425 50 275 18 472 224 351
Cuts at: 2877 2889 3096 4633 5004 5196 5715 5752 6015
Size: 12 207 1537 371 192 519 37 263
Cuts at: 6015 6280 7169 8798 8
Size: 265 889 1629 43
Fragments arranged by size:
1629 1537 1425 889 519 472 371 351
275 265 263 224 207 192 54 50
43 37 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2302 2526 2889 3096 4633 5004 5752
Size: 275 490 224 363 207 1537 371 748
Cuts at: 5752 6280 1537
Size: 528 4090
Fragments arranged by size:
4090 1537 748 528 490 371 363 275
224 207
EarI CTCTTCn'nnn_
Cuts at: 1353 3299 3794 4644 5021 5951 6763 1353
Size: 1946 495 850 377 930 812 3423
Fragments arranged by size:
3423 1946 930 850 812 495 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4520 7420 7794 7940 387
Size: 1064 138 2931 2900 374 146 1280
Fragments arranged by size:
2931 2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2736 3342 3555 2736
Size: 606 213 8014
Fragments arranged by size:
8014 606 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2313 2384 2765 2889 2890 4064
Size: 393 573 108 71 381 124 1 1174
Cuts at: 4064 4493 4573 5380 5688 5824 6109 6896 7493
Size: 429 80 807 308 136 285 787 597
Cuts at: 7493 8280 8548 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1174 807 787 787 597 573 429
393 381 308 285 268 136 124 108
80 71 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 4163 4844 5325 5930 2981
Size: 1182 681 481 605 5884
Fragments arranged by size:
5884 1182 681 605 481
EcoO109I rG'GnC_Cy
Cuts at: 3316 3426 3493 4168 4299 4543 4728 5080 5895
Size: 110 67 675 131 244 185 352 815
Cuts at: 5895 6104 3316
Size: 209 6045
Fragments arranged by size:
6045 815 675 352 244 209 185 131
110 67
EcoRI G'AATT_C
Cuts at: 3125 4211 6142 6520 3125
Size: 1086 1931 378 5438
Fragments arranged by size:
5438 1931 1086 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 4963 5119 2920
Size: 2043 156 6634
Fragments arranged by size:
6634 2043 156
FspI TGC'GCA
Cuts at: 7339 7339
Size: 8833
HaeII r_GCGC'y
Cuts at: 1678 2788 2913 3000 3613 4038 4114 4159 5271
Size: 1110 125 87 613 425 76 45 1112
Cuts at: 5271 5742 5766 7980 1678
Size: 471 24 2214 2531
Fragments arranged by size:
2531 2214 1112 1110 613 471 425 125
87 76 45 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 3653 3680 3685
Size: 32 135 32 1013 32 1036 27 5
Cuts at: 3685 3712 3960 3986 3992 4018 4423 4455 4904
Size: 27 248 26 6 26 405 32 449
Cuts at: 4904 4936 4952 4984 5801 5833 7474 7506 7548
Size: 32 16 32 817 32 1641 32 42
Cuts at: 7548 7580 1373
Size: 32 2626
Fragments arranged by size:
2626 1641 1036 1013 817 449 405 248
135 42 32 32 32 32 32 32
32 32 32 27 27 26 26 16
6 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4029 4351 6177 6423 669
Size: 988 281 2091 322 1826 246 3079
Fragments arranged by size:
3079 2091 1826 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8833
HpaI GTT'AAC
Cuts at: 6423 6423
Size: 8833
KasI G'GCGC_C
Cuts at: 2784 2996 4034 4110 5267 2784
Size: 212 1038 76 1157 6350
Fragments arranged by size:
6350 1157 1038 212 76
KpnI G_GTAC'C
Cuts at: 6123 6158 6123
Size: 35 8798
MfeI C'AATT_G
Cuts at: 6432 6432
Size: 8833
MluI A'CGCG_T
Cuts at: 8709 8709
Size: 8833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2527 2763 2775 4824 7947 8131 1305
Size: 1222 236 12 2049 3123 184 2007
Fragments arranged by size:
3123 2049 2007 1222 236 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5198 5717 10
Size: 54 1425 343 3366 519 3126
Fragments arranged by size:
3366 3126 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2841 3855 4590 4779 5177 6791
Size: 648 1072 603 1014 735 189 398 1614
Cuts at: 6791 7150 7309 518
Size: 359 159 2042
Fragments arranged by size:
2042 1614 1072 1014 735 648 603 398
359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2139 2772 2851 3276 3787 4137 4426 4483
Size: 533 633 79 425 511 350 289 57
Cuts at: 4483 5009 5296 5381 5708 5892 6249 6926 8074
Size: 526 287 85 327 184 357 677 1148
Cuts at: 8074 8319 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 633 533 526 511 425
357 350 327 289 287 245 184 85
79 57
NaeI GCC'GGC
Cuts at: 2095 4109 2095
Size: 2014 6819
NarI GG'CG_CC
Cuts at: 2785 2997 4035 4111 5268 2785
Size: 212 1038 76 1157 6350
Fragments arranged by size:
6350 1157 1038 212 76
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8833
NgoMIV G'CCGG_C
Cuts at: 2093 4107 2093
Size: 2014 6819
NheI G'CTAG_C
Cuts at: 1052 4372 1052
Size: 3320 5513
NotI GC'GGCC_GC
Cuts at: 5004 6280 5004
Size: 1276 7557
NsiI A_TGCA'T
Cuts at: 4499 4499
Size: 8833
NspI r_CATG'y
Cuts at: 1132 1229 2584 2782 3205 4411 4778 6191 7736
Size: 97 1355 198 423 1206 367 1413 1545
Cuts at: 7736 1132
Size: 2229
Fragments arranged by size:
2229 1545 1413 1355 1206 423 367 198
97
PasI CC'CwG_GG
Cuts at: 1348 3405 1348
Size: 2057 6776
PciI A'CATG_T
Cuts at: 4407 7732 4407
Size: 3325 5508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2042 2815 5998 1108
Size: 934 773 3183 3943
Fragments arranged by size:
3943 3183 934 773
PfoI T'CCnGG_A
Cuts at: 3633 3812 4527 4908 5796 3633
Size: 179 715 381 888 6670
Fragments arranged by size:
6670 888 715 381 179
PmeI GTTT'AAAC
Cuts at: 6137 6137
Size: 8833
PmlI CAC'GTG
Cuts at: 3019 3628 6013 3019
Size: 609 2385 5839
Fragments arranged by size:
5839 2385 609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5679 5711 6902 6934 8415 8447 5679
Size: 32 1191 32 1481 32 6065
Fragments arranged by size:
6065 1481 1191 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3316 3493 4168 4543 4728 5080 6104 3316
Size: 177 675 375 185 352 1024 6045
Fragments arranged by size:
6045 1024 675 375 352 185 177
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8833
PsiI TTA'TAA
Cuts at: 6403 6403
Size: 8833
PspOMI G'GGCC_C
Cuts at: 3207 3425 4299 5894 3207
Size: 218 874 1595 6146
Fragments arranged by size:
6146 1595 874 218
PspXI vC'TCGA_Gb
Cuts at: 3343 3343
Size: 8833
PstI C_TGCA'G
Cuts at: 830 3156 4167 4331 4431 4856 6185 830
Size: 2326 1011 164 100 425 1329 3478
Fragments arranged by size:
3478 2326 1329 1011 425 164 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8833
PvuII CAG'CTG
Cuts at: 2139 2851 4137 4426 5381 5708 2139
Size: 712 1286 289 955 327 5264
Fragments arranged by size:
5264 1286 955 712 327 289
SacI G_AGCT'C
Cuts at: 2738 3344 3557 2738
Size: 606 213 8014
Fragments arranged by size:
8014 606 213
SacII CC_GC'GG
Cuts at: 2773 5010 2773
Size: 2237 6596
SalI G'TCGA_C
Cuts at: 1936 6175 1936
Size: 4239 4594
SapI GCTCTTCn'nnn_
Cuts at: 3299 4644 3299
Size: 1345 7488
SbfI CC_TGCA'GG
Cuts at: 4856 6185 4856
Size: 1329 7504
ScaI AGT'ACT
Cuts at: 7081 8793 7081
Size: 1712 7121
SexAI A'CCwGG_T
Cuts at: 5643 6115 5643
Size: 472 8361
SfcI C'TryA_G
Cuts at: 826 1045 3152 3754 4163 4294 4327 4427 4852
Size: 219 2107 602 409 131 33 100 425
Cuts at: 4852 6181 7316 7997 8188 826
Size: 1329 1135 681 191 1471
Fragments arranged by size:
2107 1471 1329 1135 681 602 425 409
219 191 131 100 33
SfoI GGC'GCC
Cuts at: 2786 2998 4036 4112 5269 2786
Size: 212 1038 76 1157 6350
Fragments arranged by size:
6350 1157 1038 212 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8833
SmaI CCC'GGG
Cuts at: 1872 2084 4304 5829 5875 6160 1872
Size: 212 2220 1525 46 285 4545
Fragments arranged by size:
4545 2220 1525 285 212 46
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3343 3547 3998 5147 6937 7838
Size: 197 926 1400 204 451 1149 1790 901
Cuts at: 7838 8100 820
Size: 262 1553
Fragments arranged by size:
1790 1553 1400 1149 926 901 451 262
204 197
SnaBI TAC'GTA
Cuts at: 493 6624 493
Size: 6131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8833
SphI G_CATG'C
Cuts at: 1132 2782 3205 4778 6191 1132
Size: 1650 423 1573 1413 3774
Fragments arranged by size:
3774 1650 1573 1413 423
SrfI GCCC'GGGC
Cuts at: 5829 5829
Size: 8833
SspI AAT'ATT
Cuts at: 5 52 4253 6757 5
Size: 47 4201 2504 2081
Fragments arranged by size:
4201 2504 2081 47
StuI AGG'CCT
Cuts at: 1517 2024 3821 4244 4788 4850 1517
Size: 507 1797 423 544 62 5500
Fragments arranged by size:
5500 1797 544 507 423 62
StyI C'CwwG_G
Cuts at: 513 1065 2057 3061 3508 5263 5718 6272 513
Size: 552 992 1004 447 1755 455 554 3074
Fragments arranged by size:
3074 1755 1004 992 554 552 455 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3173 3323 3374 3575 4416 6860 6877
Size: 1255 1593 150 51 201 841 2444 17
Cuts at: 6877 7030 7215 7660 325
Size: 153 185 445 1498
Fragments arranged by size:
2444 1593 1498 1255 841 445 201 185
153 150 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7079 8791 96
Size: 274 80 33 51 1443 5102 1712 138
Fragments arranged by size:
5102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 8793 8825 3629
Size: 32 5132 32 3637
Fragments arranged by size:
5132 3637 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4052 4172 4382 1316
Size: 2736 120 210 5767
Fragments arranged by size:
5767 2736 210 120
XbaI T'CTAG_A
Cuts at: 6169 6169
Size: 8833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3118 5206 3118
Size: 2088 6745
XhoI C'TCGA_G
Cuts at: 1943 3343 1943
Size: 1400 7433
XmaI C'CCGG_G
Cuts at: 1870 2082 4302 5827 5873 6158 1870
Size: 212 2220 1525 46 285 4545
Fragments arranged by size:
4545 2220 1525 285 212 46
XmnI GAAnn'nnTTC
Cuts at: 4211 6962 4211
Size: 2751 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4384 6641 276
Size: 53 83 186 936 2850 2257 2468
Fragments arranged by size:
2850 2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbvCI BsiWI BstXI BstZ17I Bsu36I
EcoRV FseI FspAI NruI PacI PsrI RsrII SanDI
SfiI SwaI