Restriction map for
pFN21AB5626
(Circular) (Six-base) MAPSORT of: pFN21AB5626.seq Check: 366 from: 1 to: 6091
pFN21AB5626 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 3901 278
Size: 53 83 186 936 1642 723 2468
Fragments arranged by size:
2468 1642 936 723 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 2454 4154 5538 5806 1632
Size: 804 18 1700 1384 268 1917
Fragments arranged by size:
1917 1700 1384 804 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 2390 1166
Size: 1224 4867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaI G_GGCC'C
Cuts at: 3167 3167
Size: 6091
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2620 2912 3416 1870
Size: 73 677 292 504 4545
Fragments arranged by size:
4545 677 504 292 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 3167 3410 1391
Size: 711 1065 243 4072
Fragments arranged by size:
4072 1065 711 243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 2828 928
Size: 971 543 386 4191
Fragments arranged by size:
4191 971 543 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2737 2771 2831 2865 4282 4316 1903
Size: 34 800 34 60 34 1417 34 3678
Fragments arranged by size:
3678 1417 800 60 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2488 3983 5193 2488
Size: 1495 1210 3386
Fragments arranged by size:
3386 1495 1210
BclI T'GATC_A
Cuts at: 1548 2346 1548
Size: 798 5293
BfrBI ATG'CAT
Cuts at: 3324 3324
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3356 6086 3356
Size: 2730 3361
BlpI GC'TnA_GC
Cuts at: 3288 3508 3288
Size: 220 5871
Bme1580I G_kGCm'C
Cuts at: 1790 2682 3167 4152 5308 1790
Size: 892 485 985 1156 2573
Fragments arranged by size:
2573 1156 985 892 485
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2535 3168 3822
Size: 416 53 83 399 24 1118 633 654
Cuts at: 3822 4780 442
Size: 958 1753
Fragments arranged by size:
1753 1118 958 654 633 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 2676 2944 3088 4751 1239
Size: 1107 330 268 144 1663 2579
Fragments arranged by size:
2579 1663 1107 330 268 144
Bpu10I CC'TnA_GC
Cuts at: 3293 3293
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2941 3882 493
Size: 711 1737 941 2702
Fragments arranged by size:
2702 1737 941 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3175 3898 4280 4915
Size: 53 83 186 936 1642 723 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1642 1451 936 723 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 2702 2732 2942
Size: 30 165 30 1027 30 242 30 210
Cuts at: 2942 2972 3037 3067 1178
Size: 30 65 30 4202
Fragments arranged by size:
4202 1027 242 210 165 65 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2185 2726 3249 1186
Size: 999 541 523 4028
Fragments arranged by size:
4028 999 541 523
BseYI C'CCAG_C
Cuts at: 2101 5294 5790 2101
Size: 3193 496 2402
Fragments arranged by size:
3193 2402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2682 3167 3410 4152 4237 5308
Size: 399 312 580 485 243 742 85 1071
Cuts at: 5308 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 580 485 399 312 243
85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 3446 844
Size: 1297 1305 3489
Fragments arranged by size:
3489 1305 1297
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2877 4586 4760 65
Size: 1183 1629 1709 174 1396
Fragments arranged by size:
1709 1629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2744 4735 4922 1700
Size: 110 934 1991 187 2869
Fragments arranged by size:
2869 1991 934 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 3198 4151 5163 3198
Size: 953 1012 4126
Fragments arranged by size:
4126 1012 953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3314 3330 3356 3421 3788 4173 4190 5642
Size: 1342 16 26 65 367 385 17 1452
Cuts at: 5642 5728 5740 6086 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1342 385 367 346 86 65
26 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 3218 3218
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2082 513
Size: 552 1017 4522
Fragments arranged by size:
4522 1017 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2740 487
Size: 683 56 1514 3838
Fragments arranged by size:
3838 1514 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 4427 6056
Size: 54 1425 50 275 18 1708 889 1629
Cuts at: 6056 8
Size: 43
Fragments arranged by size:
1708 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2720 4021 1353
Size: 1367 1301 3423
Fragments arranged by size:
3423 1367 1301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 4678 5052 5198 387
Size: 1064 138 1134 1955 374 146 1280
Fragments arranged by size:
1955 1280 1134 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2436 2454 2676 2944 3088 4154
Size: 393 714 90 18 222 268 144 1066
Cuts at: 4154 4751 5538 5806 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1066 787 714 597 393 268 268
222 144 90 18
EcoO109I rG'GnC_Cy
Cuts at: 3163 3194 3163
Size: 31 6060
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2357 2389 2521 2553 4732
Size: 32 135 32 785 32 132 32 2179
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2179 785 135 132 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2417 2783 4049 4408 4567 518
Size: 648 1224 27 366 1266 359 159 2042
Fragments arranged by size:
2042 1266 1224 648 366 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2084 2815 2926 3066 3507 4184 5332 5577
Size: 478 731 111 140 441 677 1148 245
Cuts at: 5577 1606
Size: 2120
Fragments arranged by size:
2120 1148 731 677 478 441 245 140
111
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 2443 387
Size: 2056 4035
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NsiI A_TGCA'T
Cuts at: 3326 3326
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3227 3328 3449 4994 1132
Size: 97 1998 101 121 1545 2229
Fragments arranged by size:
2229 1998 1545 121 101 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 3163 3163
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2152 3443 830
Size: 1322 1291 3478
Fragments arranged by size:
3478 1322 1291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2926 3066 2926
Size: 140 5951
SacII CC_GC'GG
Cuts at: 2085 2085
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2148 3439 4574 5255 5446 826
Size: 219 1103 1291 1135 681 191 1471
Fragments arranged by size:
1471 1291 1135 1103 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2620 4195 5096 5358 820
Size: 197 926 677 1575 901 262 1553
Fragments arranged by size:
1575 1553 926 901 677 262 197
SnaBI TAC'GTA
Cuts at: 493 2941 3882 493
Size: 2448 941 2702
Fragments arranged by size:
2702 2448 941
SpeI A'CTAG_T
Cuts at: 152 2161 152
Size: 2009 4082
SphI G_CATG'C
Cuts at: 1132 3227 3328 3449 1132
Size: 2095 101 121 3774
Fragments arranged by size:
3774 2095 121 101
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 3530 513
Size: 552 2465 3074
Fragments arranged by size:
3074 2465 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2841 3062 4118 4135 4288 4473 4918
Size: 1255 1261 221 1056 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1498 1261 1255 1056 445 221 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2666 4337 6049
Size: 274 80 33 51 1443 689 1671 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
1712 1671 1443 689 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 3002 3034 3037 3069 6051 6083 2430
Size: 32 540 32 3 32 2982 32 2438
Fragments arranged by size:
2982 2438 540 32 32 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3373 3373
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 2620 1943
Size: 677 5414
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2567 4220 2567
Size: 1653 4438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 3899 276
Size: 53 83 186 936 1642 723 2468
Fragments arranged by size:
2468 1642 936 723 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI