Restriction map for
pFN21AB5625
(Circular) (Six-base) MAPSORT of: pFN21AB5625.seq Check: 7762 from: 1 to: 8464
pFN21AB5625 8464 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6274 278
Size: 53 83 186 936 4738 2468
Fragments arranged by size:
4738 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2487 4956 5807 1937
Size: 550 2469 851 4594
Fragments arranged by size:
4594 2469 851 550
Acc65I G'GTAC_C
Cuts at: 5785 5785
Size: 8464
AclI AA'CG_TT
Cuts at: 1563 5491 6591 6964 1563
Size: 3928 1100 373 3063
Fragments arranged by size:
3928 3063 1100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2982 4034 4148 6527 7911 8179 1632
Size: 1350 1052 114 2379 1384 268 1917
Fragments arranged by size:
2379 1917 1384 1350 1052 268 114
AflII C'TTAA_G
Cuts at: 820 1017 3815 820
Size: 197 2798 5469
Fragments arranged by size:
5469 2798 197
AflIII A'CryG_T
Cuts at: 1203 7363 8340 1203
Size: 6160 977 1327
Fragments arranged by size:
6160 1327 977
AgeI A'CCGG_T
Cuts at: 7295 7295
Size: 8464
AhdI GACnn_n'nnGTC
Cuts at: 7193 7193
Size: 8464
AleI CACnn'nnGTG
Cuts at: 1166 4604 1166
Size: 3438 5026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5157 1184
Size: 3973 4491
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 2536
Size: 32 8432
AlwNI CAG_nnn'CTG
Cuts at: 3352 3372 4246 4943 6207 7779 3352
Size: 20 874 697 1264 1572 4037
Fragments arranged by size:
4037 1572 1264 874 697 20
ApaLI G'TGCA_C
Cuts at: 6521 7677 6521
Size: 1156 7308
ApoI r'AATT_y
Cuts at: 1493 3170 4296 4922 5773 5999 6151 8260 1493
Size: 1677 1126 626 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 1677 1126 851 626 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4515 7018 160
Size: 872 3483 2503 1606
Fragments arranged by size:
3483 2503 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8464
AvaI C'yCGr_G
Cuts at: 1870 1943 3094 3806 5789 1870
Size: 73 1151 712 1983 4545
Fragments arranged by size:
4545 1983 1151 712 73
AvrII C'CTAG_G
Cuts at: 4789 4789
Size: 8464
BamHI G'GATC_C
Cuts at: 5794 6161 5794
Size: 367 8097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3525 5785 7240 618
Size: 325 217 627 1738 2260 1455 1842
Fragments arranged by size:
2260 1842 1738 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2930 3509 5203 5783 1391
Size: 1539 579 1694 580 4072
Fragments arranged by size:
4072 1694 1539 580 579
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 4079 4179 5364 5441 928
Size: 971 171 2009 100 1185 77 3951
Fragments arranged by size:
3951 2009 1185 971 171 100 77
BbvCI CC'TCA_GC
Cuts at: 2117 2117
Size: 8464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2508 2542 6655 6689 1903
Size: 34 571 34 4113 34 3678
Fragments arranged by size:
4113 3678 571 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 6356 7566 3722
Size: 2634 1210 4620
Fragments arranged by size:
4620 2634 1210
BclI T'GATC_A
Cuts at: 1548 2389 3067 3841 5602 1548
Size: 841 678 774 1761 4410
Fragments arranged by size:
4410 1761 841 774 678
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7075 136
Size: 107 122 71 631 605 5403 1525
Fragments arranged by size:
5403 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3752 8459 3752
Size: 4707 3757
BlpI GC'TnA_GC
Cuts at: 5881 5881
Size: 8464
Bme1580I G_kGCm'C
Cuts at: 1790 5317 6525 7681 1790
Size: 3527 1208 1156 2573
Fragments arranged by size:
3527 2573 1208 1156
BmgBI CAC'GTC
Cuts at: 1318 3269 1318
Size: 1951 6513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 6195 7153
Size: 416 53 83 399 24 2003 2775 958
Cuts at: 7153 442
Size: 1753
Fragments arranged by size:
2775 2003 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 4078 5468 2083
Size: 1995 1390 5079
Fragments arranged by size:
5079 1995 1390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2713 3135 3374 4199 4423 7124 1239
Size: 1474 422 239 825 224 2701 2579
Fragments arranged by size:
2701 2579 1474 825 422 239 224
Bpu10I CC'TnA_GC
Cuts at: 2117 2257 2929 2117
Size: 140 672 7652
Fragments arranged by size:
7652 672 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 2446 3473 5594 6589 7454 7752 2443
Size: 3 1027 2121 995 865 298 3155
Fragments arranged by size:
3155 2121 1027 995 865 298 3
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3350 5036 7127 882
Size: 617 1851 1686 2091 2219
Fragments arranged by size:
2219 2091 1851 1686 617
BsaAI yAC'GTr
Cuts at: 493 1204 6255 493
Size: 711 5051 2702
Fragments arranged by size:
5051 2702 711
BsaBI GATnn'nnATC
Cuts at: 8458 8458
Size: 8464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5221 6271 6653 7288
Size: 53 83 186 936 3688 1050 382 635
Cuts at: 7288 275
Size: 1451
Fragments arranged by size:
3688 1451 1050 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3531 3957 5349 6897 7295 7569 7716 8127 3531
Size: 426 1392 1548 398 274 147 411 3868
Fragments arranged by size:
3868 1548 1392 426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3470 3500 3565 3595 3637
Size: 30 165 30 2067 30 65 30 42
Cuts at: 3637 3667 1178
Size: 30 5975
Fragments arranged by size:
5975 2067 165 65 42 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3034 3491 3645 1186
Size: 1848 457 154 6005
Fragments arranged by size:
6005 1848 457 154
BseYI C'CCAG_C
Cuts at: 3219 4077 7667 8163 3219
Size: 858 3590 496 3520
Fragments arranged by size:
3590 3520 858 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3452 1163
Size: 573 1716 6175
Fragments arranged by size:
6175 1716 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5914 6675 7703 1540
Size: 275 186 3913 761 1028 2301
Fragments arranged by size:
3913 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2930 3617 3886 4638 4821 6525 6610 7681 2930
Size: 687 269 752 183 1704 85 1071 3713
Fragments arranged by size:
3713 1704 1071 752 687 269 183 85
BsmI GAATG_Cn'
Cuts at: 2077 2640 5620 5975 6068 2077
Size: 563 2980 355 93 4473
Fragments arranged by size:
4473 2980 563 355 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2930 3509 3617 3886 4638 4821 5203
Size: 399 1140 579 108 269 752 183 382
Cuts at: 5203 5317 5783 6525 6610 7681 1391
Size: 114 466 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 752 742 579 466 399
382 269 183 114 108 85
BspEI T'CCGG_A
Cuts at: 5349 8127 5349
Size: 2778 5686
BspHI T'CATG_A
Cuts at: 3501 5662 6353 8083 3501
Size: 2161 691 1730 3882
Fragments arranged by size:
3882 2161 1730 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5349 5447 5819 844
Size: 4505 98 372 3489
Fragments arranged by size:
4505 3489 372 98
BsrBI CCG'CTC
Cuts at: 1394 1823 6351 1394
Size: 429 4528 3507
Fragments arranged by size:
4528 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3777 6959 7133 65
Size: 1183 2529 3182 174 1396
Fragments arranged by size:
3182 2529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3291 7108 7295 1700
Size: 110 1481 3817 187 2869
Fragments arranged by size:
3817 2869 1481 187 110
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8464
BssSI C'ACGA_G
Cuts at: 3009 6524 7536 3009
Size: 3515 1012 3937
Fragments arranged by size:
3937 3515 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8464
BstBI TT'CG_AA
Cuts at: 7268 7268
Size: 8464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8464
BstYI r'GATC_y
Cuts at: 1972 2872 3161 3752 5709 5794 6161 6546 6563
Size: 900 289 591 1957 85 367 385 17
Cuts at: 6563 8015 8101 8113 8459 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1957 1452 900 591 385 367 346
289 86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 3208 3208
Size: 8464
BtgI C'CryG_G
Cuts at: 513 1065 2004 3420 4839 513
Size: 552 939 1416 1419 4138
Fragments arranged by size:
4138 1419 1416 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5676 487
Size: 683 56 4450 3275
Fragments arranged by size:
4450 3275 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4750 5980 6774 6794 3595
Size: 1155 1230 794 20 5265
Fragments arranged by size:
5265 1230 1155 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5446 5481 529
Size: 35 4882 35 3512
Fragments arranged by size:
4882 3512 35 35
DraI TTT'AAA
Cuts at: 2152 4485 5768 2152
Size: 2333 1283 4848
Fragments arranged by size:
4848 2333 1283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7471 809
Size: 507 6155 1802
Fragments arranged by size:
6155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2401 2535 4842
Size: 54 1425 50 275 18 571 134 2307
Cuts at: 4842 5392 5911 6800 8429 8
Size: 550 519 889 1629 43
Fragments arranged by size:
2307 1629 1425 889 571 550 519 275
134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5911 1537
Size: 275 4099 4090
Fragments arranged by size:
4099 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2140 2371 4124 6394 1353
Size: 651 136 231 1753 2270 3423
Fragments arranged by size:
3423 2270 1753 651 231 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7051 7425 7571 387
Size: 1064 138 5462 374 146 1280
Fragments arranged by size:
5462 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2928 2928
Size: 8464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2713 2982 3135 3374 4034 4148 4199
Size: 393 1081 269 153 239 660 114 51
Cuts at: 4199 4423 6527 7124 7911 8179 1239
Size: 224 2104 597 787 268 1524
Fragments arranged by size:
2104 1524 1081 787 660 597 393 269
268 239 224 153 114 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 3200
Size: 8464
EcoO109I rG'GnC_Cy
Cuts at: 2418 2418
Size: 8464
EcoRI G'AATT_C
Cuts at: 5773 6151 5773
Size: 378 8086
EcoRV GAT'ATC
Cuts at: 2366 2366
Size: 8464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2625 4246 5377 2625
Size: 1621 1131 5712
Fragments arranged by size:
5712 1621 1131
FspI TGC'GCA
Cuts at: 6970 6970
Size: 8464
HaeII r_GCGC'y
Cuts at: 1678 2660 7611 1678
Size: 982 4951 2531
Fragments arranged by size:
4951 2531 982
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 3271 3303 3334
Size: 32 135 32 1106 32 561 32 31
Cuts at: 3334 3366 3464 3496 3565 3597 3931 3963 4841
Size: 32 98 32 69 32 334 32 878
Cuts at: 4841 4873 5060 5092 5439 5471 5617 5649 7105
Size: 32 187 32 347 32 146 32 1456
Cuts at: 7105 7137 7179 7211 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1456 1106 878 561 347 334 187
146 135 98 69 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 4471 5808 6054 669
Size: 988 281 735 1798 1337 246 3079
Fragments arranged by size:
3079 1798 1337 988 735 281 246
HindIII A'AGCT_T
Cuts at: 748 3684 4016 748
Size: 2936 332 5196
Fragments arranged by size:
5196 2936 332
HpaI GTT'AAC
Cuts at: 2673 4471 6054 2673
Size: 1798 1583 5083
Fragments arranged by size:
5083 1798 1583
KpnI G_GTAC'C
Cuts at: 5789 5789
Size: 8464
MfeI C'AATT_G
Cuts at: 4087 6063 4087
Size: 1976 6488
MluI A'CGCG_T
Cuts at: 8340 8340
Size: 8464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2351 2492 3849 4128 7578 7762 1305
Size: 1046 141 1357 279 3450 184 2007
Fragments arranged by size:
3450 2007 1357 1046 279 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2537 4844 5394 10
Size: 54 1425 343 705 2307 550 3080
Fragments arranged by size:
3080 2307 1425 705 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 4604 5459 6422 6781 6940 518
Size: 648 1315 2123 855 963 359 159 2042
Fragments arranged by size:
2123 2042 1315 963 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2803 4081 4604 4806 5880 6557 7705 7950
Size: 1197 1278 523 202 1074 677 1148 245
Cuts at: 7950 1606
Size: 2120
Fragments arranged by size:
2120 1278 1197 1148 1074 677 523 245
202
NcoI C'CATG_G
Cuts at: 513 1065 2004 4839 513
Size: 552 939 2835 4138
Fragments arranged by size:
4138 2835 939 552
NdeI CA'TA_TG
Cuts at: 387 3079 387
Size: 2692 5772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8464
NotI GC'GGCC_GC
Cuts at: 5911 5911
Size: 8464
NspI r_CATG'y
Cuts at: 1132 1229 2608 5822 7367 1132
Size: 97 1379 3214 1545 2229
Fragments arranged by size:
3214 2229 1545 1379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8464
PciI A'CATG_T
Cuts at: 7363 7363
Size: 8464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8464
PfoI T'CCnGG_A
Cuts at: 5232 5232
Size: 8464
PmeI GTTT'AAAC
Cuts at: 5768 5768
Size: 8464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6533 6565 8046 8078 6533
Size: 32 1481 32 6919
Fragments arranged by size:
6919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4089 1613
Size: 2476 5988
PsiI TTA'TAA
Cuts at: 4881 4929 6034 4881
Size: 48 1105 7311
Fragments arranged by size:
7311 1105 48
PspXI vC'TCGA_Gb
Cuts at: 3806 3806
Size: 8464
PstI C_TGCA'G
Cuts at: 830 3465 5816 830
Size: 2635 2351 3478
Fragments arranged by size:
3478 2635 2351
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8464
PvuII CAG'CTG
Cuts at: 2803 4081 4604 4806 2803
Size: 1278 523 202 6461
Fragments arranged by size:
6461 1278 523 202
SacI G_AGCT'C
Cuts at: 2930 2930
Size: 8464
SalI G'TCGA_C
Cuts at: 1936 5806 1936
Size: 3870 4594
SapI GCTCTTCn'nnn_
Cuts at: 2004 2371 2004
Size: 367 8097
SbfI CC_TGCA'GG
Cuts at: 5816 5816
Size: 8464
ScaI AGT'ACT
Cuts at: 2726 6712 8424 2726
Size: 3986 1712 2766
Fragments arranged by size:
3986 2766 1712
SfcI C'TryA_G
Cuts at: 826 1045 2758 3461 5812 6947 7628 7819 826
Size: 219 1713 703 2351 1135 681 191 1471
Fragments arranged by size:
2351 1713 1471 1135 703 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8464
SgrAI Cr'CCGG_yG
Cuts at: 1700 3291 1700
Size: 1591 6873
SmaI CCC'GGG
Cuts at: 1872 5791 1872
Size: 3919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2422 2461 3452 3806 3815 5609
Size: 197 926 479 39 991 354 9 1794
Cuts at: 5609 6568 7469 7731 820
Size: 959 901 262 1553
Fragments arranged by size:
1794 1553 991 959 926 901 479 354
262 197 39 9
SnaBI TAC'GTA
Cuts at: 493 6255 493
Size: 5762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8464
SphI G_CATG'C
Cuts at: 1132 2608 5822 1132
Size: 1476 3214 3774
Fragments arranged by size:
3774 3214 1476
SspI AAT'ATT
Cuts at: 5 52 2311 5098 5401 6388 5
Size: 47 2259 2787 303 987 2081
Fragments arranged by size:
2787 2259 2081 987 303 47
StuI AGG'CCT
Cuts at: 1517 3206 4680 5003 1517
Size: 1689 1474 323 4978
Fragments arranged by size:
4978 1689 1474 323
StyI C'CwwG_G
Cuts at: 513 1065 2004 4002 4789 4839 5903 513
Size: 552 939 1998 787 50 1064 3074
Fragments arranged by size:
3074 1998 1064 939 787 552 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5453 6491 6508 6661 6846 7291 325
Size: 1255 3873 1038 17 153 185 445 1498
Fragments arranged by size:
3873 1498 1255 1038 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 2781 6710
Size: 274 80 33 51 1443 747 57 3929
Cuts at: 6710 8422 96
Size: 1712 138
Fragments arranged by size:
3929 1712 1443 747 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3637 3669 4546 4578 8424 8456 3637
Size: 32 877 32 3846 32 3645
Fragments arranged by size:
3846 3645 877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 3348 1316
Size: 1167 865 6432
Fragments arranged by size:
6432 1167 865
XbaI T'CTAG_A
Cuts at: 4210 4283 5800 4210
Size: 73 1517 6874
Fragments arranged by size:
6874 1517 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3010 4606 3010
Size: 1596 6868
XhoI C'TCGA_G
Cuts at: 1943 3806 1943
Size: 1863 6601
XmaI C'CCGG_G
Cuts at: 1870 5789 1870
Size: 3919 4545
XmnI GAAnn'nnTTC
Cuts at: 2218 4967 6593 2218
Size: 2749 1626 4089
Fragments arranged by size:
4089 2749 1626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6272 276
Size: 53 83 186 936 4738 2468
Fragments arranged by size:
4738 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI