Restriction map for
pFN21AB5624
(Circular) (Six-base) MAPSORT of: pFN21AB5624.seq Check: 6951 from: 1 to: 11563
pFN21AB5624 11563 bp
With 182 enzymes: SgfI *
August 3, 2016 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6935 7059 6935
Size: 124 11439
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9373 278
Size: 53 83 186 936 7837 2468
Fragments arranged by size:
7837 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5156 5535 6186 8906 1937
Size: 3219 379 651 2720 4594
Fragments arranged by size:
4594 3219 2720 651 379
Acc65I G'GTAC_C
Cuts at: 8884 8884
Size: 11563
AclI AA'CG_TT
Cuts at: 1563 9690 10063 1563
Size: 8127 373 3063
Fragments arranged by size:
8127 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2732 3854 6707 6803 7181 7363 7409
Size: 551 549 1122 2853 96 378 182 46
Cuts at: 7409 8690 9626 11010 11278 1632
Size: 1281 936 1384 268 1917
Fragments arranged by size:
2853 1917 1384 1281 1122 936 551 549
378 268 182 96 46
AfeI AGC'GCT
Cuts at: 2234 4382 6498 2234
Size: 2148 2116 7299
Fragments arranged by size:
7299 2148 2116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11366
AflIII A'CryG_T
Cuts at: 1203 2559 3052 3333 3598 5482 6219 8325 10462
Size: 1356 493 281 265 1884 737 2106 2137
Cuts at: 10462 11439 1203
Size: 977 1327
Fragments arranged by size:
2137 2106 1884 1356 1327 977 737 493
281 265
AgeI A'CCGG_T
Cuts at: 10394 10394
Size: 11563
AhdI GACnn_n'nnGTC
Cuts at: 10292 10292
Size: 11563
AleI CACnn'nnGTG
Cuts at: 1166 4217 8075 1166
Size: 3051 3858 4654
Fragments arranged by size:
4654 3858 3051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3948 6083 6587 7993 1184
Size: 2764 2135 504 1406 4754
Fragments arranged by size:
4754 2764 2135 1406 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8417 8449 8417
Size: 32 11531
AlwNI CAG_nnn'CTG
Cuts at: 3367 3392 4741 5681 5887 6680 7204 8659 9306
Size: 25 1349 940 206 793 524 1455 647
Cuts at: 9306 10878 3367
Size: 1572 4052
Fragments arranged by size:
4052 1572 1455 1349 940 793 647 524
206 25
ApaI G_GGCC'C
Cuts at: 4812 6316 6632 6750 4812
Size: 1504 316 118 9625
Fragments arranged by size:
9625 1504 316 118
ApaLI G'TGCA_C
Cuts at: 3448 6320 9620 10776 3448
Size: 2872 3300 1156 4235
Fragments arranged by size:
4235 3300 2872 1156
ApoI r'AATT_y
Cuts at: 1493 4627 6801 6818 7739 8872 9098 9250 11359
Size: 3134 2174 17 921 1133 226 152 2109
Cuts at: 11359 1493
Size: 1697
Fragments arranged by size:
3134 2174 2109 1697 1133 921 226 152
17
AseI AT'TA_AT
Cuts at: 160 1032 10117 160
Size: 872 9085 1606
Fragments arranged by size:
9085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11563
AvaI C'yCGr_G
Cuts at: 1870 1943 3126 3644 4116 5255 5915 8888 1870
Size: 73 1183 518 472 1139 660 2973 4545
Fragments arranged by size:
4545 2973 1183 1139 660 518 472 73
BamHI G'GATC_C
Cuts at: 4059 7383 7756 8893 9260 4059
Size: 3324 373 1137 367 6362
Fragments arranged by size:
6362 3324 1137 373 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 3199 4685 6363 6584
Size: 325 217 627 288 1124 1486 1678 221
Cuts at: 6584 6829 8578 8884 10339 618
Size: 245 1749 306 1455 1842
Fragments arranged by size:
1842 1749 1678 1486 1455 1124 627 325
306 288 245 221 217
BanII G_rGCy'C
Cuts at: 1391 3471 3494 4317 4477 4812 4908 5100 5264
Size: 2080 23 823 160 335 96 192 164
Cuts at: 5264 5313 5354 5562 6316 6632 6639 6750 6892
Size: 49 41 208 754 316 7 111 142
Cuts at: 6892 8134 8181 8253 8882 1391
Size: 1242 47 72 629 4072
Fragments arranged by size:
4072 2080 1242 823 754 629 335 316
208 192 164 160 142 111 96 72
49 47 41 23 7
BbeI G_GCGC'C
Cuts at: 2079 3203 4689 6588 8582 2079
Size: 1124 1486 1899 1994 5060
Fragments arranged by size:
5060 1994 1899 1486 1124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 2998 3737 4627 4893 5616 6695
Size: 971 287 812 739 890 266 723 1079
Cuts at: 6695 8746 928
Size: 2051 3745
Fragments arranged by size:
3745 2051 1079 971 890 812 739 723
287 266
BbvCI CC'TCA_GC
Cuts at: 4371 8048 4371
Size: 3677 7886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2827 2861 8427 8461 8576 8610 9754
Size: 34 890 34 5566 34 115 34 1144
Cuts at: 9754 9788 1903
Size: 34 3678
Fragments arranged by size:
5566 3678 1144 890 115 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6464 9455 10665 6464
Size: 2991 1210 7362
Fragments arranged by size:
7362 2991 1210
BclI T'GATC_A
Cuts at: 1548 7311 8145 1548
Size: 5763 834 4966
Fragments arranged by size:
5763 4966 834
BfrBI ATG'CAT
Cuts at: 2203 2203
Size: 11563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3290 5030 6030
Size: 107 122 71 631 605 1618 1740 1000
Cuts at: 6030 8120 8557 10174 136
Size: 2090 437 1617 1525
Fragments arranged by size:
2090 1740 1618 1617 1525 1000 631 605
437 122 107 71
BglII A'GATC_T
Cuts at: 2511 4953 11558 2511
Size: 2442 6605 2516
Fragments arranged by size:
6605 2516 2442
BlpI GC'TnA_GC
Cuts at: 4746 4902 6083 6252 8980 4746
Size: 156 1181 169 2728 7329
Fragments arranged by size:
7329 2728 1181 169 156
Bme1580I G_kGCm'C
Cuts at: 1790 3452 4812 5754 6316 6324 6632 6750 7339
Size: 1662 1360 942 562 8 308 118 589
Cuts at: 7339 9624 10780 1790
Size: 2285 1156 2573
Fragments arranged by size:
2573 2285 1662 1360 1156 942 589 562
308 118 8
BmgBI CAC'GTC
Cuts at: 1318 3055 3883 5050 7282 1318
Size: 1737 828 1167 2232 5599
Fragments arranged by size:
5599 2232 1737 1167 828
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3199 5468 6421
Size: 416 53 83 399 24 1782 2269 953
Cuts at: 6421 7420 8425 9294 10252 442
Size: 999 1005 869 958 1753
Fragments arranged by size:
2269 1782 1753 1005 999 958 953 869
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11563
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 4771 5119 6433 7012 2641
Size: 2130 348 1314 579 7192
Fragments arranged by size:
7192 2130 1314 579 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4002 4094 5540 5591 5909 6428 6638 7019
Size: 2763 92 1446 51 318 519 210 381
Cuts at: 7019 7208 7322 8046 8330 8837 8846 10223 1239
Size: 189 114 724 284 507 9 1377 2579
Fragments arranged by size:
2763 2579 1446 1377 724 519 507 381
318 284 210 189 114 92 51 9
Bpu10I CC'TnA_GC
Cuts at: 2236 4371 5909 6633 8048 2236
Size: 2135 1538 724 1415 5751
Fragments arranged by size:
5751 2135 1538 1415 724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2837 3260 3770 4892 4970 5330 6419 9688
Size: 753 423 510 1122 78 360 1089 3269
Cuts at: 9688 10553 10851 2084
Size: 865 298 2796
Fragments arranged by size:
3269 2796 1122 1089 865 753 510 423
360 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3056 3608 5193 6011 6380 7691 8379
Size: 617 1557 552 1585 818 369 1311 688
Cuts at: 8379 8433 8684 8813 10226 882
Size: 54 251 129 1413 2219
Fragments arranged by size:
2219 1585 1557 1413 1311 818 688 617
552 369 251 129 54
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3375 3601 3634 4267 5483 6106
Size: 711 1518 653 226 33 633 1216 623
Cuts at: 6106 7663 8326 8458 9354 493
Size: 1557 663 132 896 2702
Fragments arranged by size:
2702 1557 1518 1216 896 711 663 653
633 623 226 132 33
BsaBI GATnn'nnATC
Cuts at: 4208 4952 6512 11557 4208
Size: 744 1560 5045 4214
Fragments arranged by size:
5045 4214 1560 744
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3200 4686 6585
Size: 53 83 186 936 543 1124 1486 1899
Cuts at: 6585 8579 9370 9752 10387 275
Size: 1994 791 382 635 1451
Fragments arranged by size:
1994 1899 1486 1451 1124 936 791 635
543 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2764 5334 5820 8778 9996 10394 10668 10815 11226
Size: 2570 486 2958 1218 398 274 147 411
Cuts at: 11226 2764
Size: 3101
Fragments arranged by size:
3101 2958 2570 1218 486 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3320 3350 4388 4418 5138
Size: 30 165 30 1917 30 1038 30 720
Cuts at: 5138 5168 5228 5258 8417 8447 1178
Size: 30 60 30 3159 30 4294
Fragments arranged by size:
4294 3159 1917 1038 720 165 60 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 3773 5738 6146 6602 6749 7886 7904
Size: 843 1744 1965 408 456 147 1137 18
Cuts at: 7904 7907 8095 8098 8438 8581 1186
Size: 3 188 3 340 143 4168
Fragments arranged by size:
4168 1965 1744 1137 843 456 408 340
188 147 143 18 3 3
BseYI C'CCAG_C
Cuts at: 3845 5413 5671 5947 6375 6575 6774 7407 7734
Size: 1568 258 276 428 200 199 633 327
Cuts at: 7734 8790 8854 10766 11262 3845
Size: 1056 64 1912 496 4146
Fragments arranged by size:
4146 1912 1568 1056 633 496 428 327
276 258 200 199 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2265 3545 5489 6026 7499 1163
Size: 573 529 1280 1944 537 1473 5227
Fragments arranged by size:
5227 1944 1473 1280 573 537 529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3285 4350 7484 8127 9013 9774
Size: 275 186 1284 1065 3134 643 886 761
Cuts at: 9774 10802 1540
Size: 1028 2301
Fragments arranged by size:
3134 2301 1284 1065 1028 886 761 643
275 186
BsiHKAI G_wGCw'C
Cuts at: 3452 4908 5100 5354 5562 5632 5910 6324 6405
Size: 1456 192 254 208 70 278 414 81
Cuts at: 6405 6606 6639 7370 8062 8072 8181 8253 9624
Size: 201 33 731 692 10 109 72 1371
Cuts at: 9624 9709 10780 3452
Size: 85 1071 4235
Fragments arranged by size:
4235 1456 1371 1071 731 692 414 278
254 208 201 192 109 85 81 72
70 33 10
BsmI GAATG_Cn'
Cuts at: 9074 9167 9074
Size: 93 11470
BsmBI CGTCTCn'nnnn_
Cuts at: 2828 4235 2828
Size: 1407 10156
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3452 3471 3494 4317 4477 4812 4908
Size: 399 1662 19 23 823 160 335 96
Cuts at: 4908 5100 5264 5313 5354 5562 5632 5754 5910
Size: 192 164 49 41 208 70 122 156
Cuts at: 5910 6316 6324 6405 6606 6632 6639 6750 6892
Size: 406 8 81 201 26 7 111 142
Cuts at: 6892 7339 7370 8062 8072 8134 8181 8253 8882
Size: 447 31 692 10 62 47 72 629
Cuts at: 8882 9624 9709 10780 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1662 1071 823 742 692 629 447
406 399 335 208 201 192 164 160
156 142 122 111 96 85 81 72
70 62 49 47 41 31 26 23
19 10 8 7
BspEI T'CCGG_A
Cuts at: 5334 8778 11226 5334
Size: 3444 2448 5671
Fragments arranged by size:
5671 3444 2448
BspHI T'CATG_A
Cuts at: 4126 7501 7995 9452 11182 4126
Size: 3375 494 1457 1730 4507
Fragments arranged by size:
4507 3375 1730 1457 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5452 6935 6945 7059 8397 8918 844
Size: 4608 1483 10 114 1338 521 3489
Fragments arranged by size:
4608 3489 1483 1338 521 114 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2158 4776 5410 8451 9450 1394
Size: 429 196 139 2618 634 3041 999 3507
Fragments arranged by size:
3507 3041 2618 999 634 429 196 139
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 8784 10058 10232 65
Size: 1183 1071 6465 1274 174 1396
Fragments arranged by size:
6465 1396 1274 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3678 6029 10207 10394 1700
Size: 110 1868 2351 4178 187 2869
Fragments arranged by size:
4178 2869 2351 1868 187 110
BsrGI T'GTAC_A
Cuts at: 96 3411 5055 7625 96
Size: 3315 1644 2570 4034
Fragments arranged by size:
4034 3315 2570 1644
BssHII G'CGCG_C
Cuts at: 1925 2032 1925
Size: 107 11456
BssSI C'ACGA_G
Cuts at: 9623 10635 9623
Size: 1012 10551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4509 6004 7960 794
Size: 3715 1495 1956 4397
Fragments arranged by size:
4397 3715 1956 1495
BstBI TT'CG_AA
Cuts at: 6663 10367 6663
Size: 3704 7859
BstEII G'GTnAC_C
Cuts at: 1184 3082 1184
Size: 1898 9665
BstXI CCAn_nnnn'nTGG
Cuts at: 3079 4495 5947 3079
Size: 1416 1452 8695
Fragments arranged by size:
8695 1452 1416
BstYI r'GATC_y
Cuts at: 1972 2416 2488 2511 2715 4059 4203 4953 6507
Size: 444 72 23 204 1344 144 750 1554
Cuts at: 6507 7383 7756 8893 9260 9645 9662 11114 11200
Size: 876 373 1137 367 385 17 1452 86
Cuts at: 11200 11212 11558 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1554 1452 1344 1137 876 750 444
385 373 367 346 204 144 86 72
23 17 12
Bsu36I CC'TnA_GG
Cuts at: 3227 8466 3227
Size: 5239 6324
BtgI C'CryG_G
Cuts at: 513 1065 3139 3312 3348 3462 3936 4350 4488
Size: 552 2074 173 36 114 474 414 138
Cuts at: 4488 4930 5313 5940 5993 6883 8677 513
Size: 442 383 627 53 890 1794 3399
Fragments arranged by size:
3399 2074 1794 890 627 552 474 442
414 383 173 138 114 53 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6742 7546 7639 487
Size: 683 56 5516 804 93 4411
Fragments arranged by size:
5516 4411 804 683 93 56
BtsI GCAGTG_nn'
Cuts at: 2248 2253 4019 6067 6070 8029 8062 9079 9873
Size: 5 1766 2048 3 1959 33 1017 794
Cuts at: 9873 9893 2248
Size: 20 3918
Fragments arranged by size:
3918 2048 1959 1766 1017 794 33 20
5 3
ClaI AT'CG_AT
Cuts at: 1554 2904 1554
Size: 1350 10213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11528
DraI TTT'AAA
Cuts at: 8867 8867
Size: 11563
DraIII CAC_nnn'GTG
Cuts at: 2440 2482 3504 4324 7179 2440
Size: 42 1022 820 2855 6824
Fragments arranged by size:
6824 2855 1022 820 42
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5207 10570 809
Size: 507 3891 5363 1802
Fragments arranged by size:
5363 3891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3110 3282
Size: 54 1425 50 275 18 1078 202 172
Cuts at: 3282 3345 3369 4347 5184 5595 6792 7206 7341
Size: 63 24 978 837 411 1197 414 135
Cuts at: 7341 8019 9010 9899 11528 8
Size: 678 991 889 1629 43
Fragments arranged by size:
1629 1425 1197 1078 991 978 889 837
678 414 411 275 202 172 135 63
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3282 4347 9010 1537
Size: 275 1470 1065 4663 4090
Fragments arranged by size:
4663 4090 1470 1065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 3233 3750 3789 4157 4218 4859 4998
Size: 1257 623 517 39 368 61 641 139
Cuts at: 4998 5359 5567 5687 5870 6506 6779 7022 7385
Size: 361 208 120 183 636 273 243 363
Cuts at: 7385 7553 7893 8527 9493 1353
Size: 168 340 634 966 3423
Fragments arranged by size:
3423 1257 966 641 636 634 623 517
368 363 361 340 273 243 208 183
168 139 120 61 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2684 4190 6446 7788 7919 10150
Size: 1064 138 1095 1506 2256 1342 131 2231
Cuts at: 10150 10524 10670 387
Size: 374 146 1280
Fragments arranged by size:
2256 2231 1506 1342 1280 1095 1064 374
146 138 131
EcoICRI GAG'CTC
Cuts at: 4906 5098 5352 5560 6637 8179 8251 4906
Size: 192 254 208 1077 1542 72 8218
Fragments arranged by size:
8218 1542 1077 254 208 192 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2732 3854 4002 4094 5540 5591
Size: 393 551 549 1122 148 92 1446 51
Cuts at: 5591 5909 6428 6638 6707 6803 7019 7181 7208
Size: 318 519 210 69 96 216 162 27
Cuts at: 7208 7322 7363 7409 8046 8330 8690 8837 8846
Size: 114 41 46 637 284 360 147 9
Cuts at: 8846 9626 10223 11010 11278 1239
Size: 780 597 787 268 1524
Fragments arranged by size:
1524 1446 1122 787 780 637 597 551
549 519 393 360 318 284 268 216
210 162 148 147 114 96 92 69
51 46 41 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 5459 6620 6935 6986 5459
Size: 1161 315 51 10036
Fragments arranged by size:
10036 1161 315 51
EcoO109I rG'GnC_Cy
Cuts at: 2993 3640 4569 4809 5734 6163 6313 6629 6746
Size: 647 929 240 925 429 150 316 117
Cuts at: 6746 6865 8656 2993
Size: 119 1791 5900
Fragments arranged by size:
5900 1791 929 925 647 429 316 240
150 119 117
EcoRI G'AATT_C
Cuts at: 6818 8872 9250 6818
Size: 2054 378 9131
Fragments arranged by size:
9131 2054 378
EcoRV GAT'ATC
Cuts at: 3670 3670
Size: 11563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4732 7564 7717 4732
Size: 2832 153 8578
Fragments arranged by size:
8578 2832 153
FseI GG_CCGG'CC
Cuts at: 6033 6033
Size: 11563
FspI TGC'GCA
Cuts at: 4466 6560 10069 4466
Size: 2094 3509 5960
Fragments arranged by size:
5960 3509 2094
HaeII r_GCGC'y
Cuts at: 1678 2079 2107 2236 3203 4384 4689 5182 5424
Size: 401 28 129 967 1181 305 493 242
Cuts at: 5424 5863 6053 6266 6500 6588 6742 6883 7042
Size: 439 190 213 234 88 154 141 159
Cuts at: 7042 7579 7639 8582 10710 1678
Size: 537 60 943 2128 2531
Fragments arranged by size:
2531 2128 1181 967 943 537 493 439
401 305 242 234 213 190 159 154
141 129 88 60 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5138 5170 5869 5901 6379
Size: 32 135 32 3566 32 699 32 478
Cuts at: 6379 6403 6411 6435 6705 6737 10204 10236 10278
Size: 24 8 24 270 32 3467 32 42
Cuts at: 10278 10310 1373
Size: 32 2626
Fragments arranged by size:
3566 3467 2626 699 478 270 135 42
32 32 32 32 32 32 32 24
24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 7213 7240 8907 9153 669
Size: 988 281 5275 27 1667 246 3079
Fragments arranged by size:
5275 3079 1667 988 281 246 27
HindIII A'AGCT_T
Cuts at: 748 3821 748
Size: 3073 8490
HpaI GTT'AAC
Cuts at: 9153 9153
Size: 11563
KasI G'GCGC_C
Cuts at: 2075 3199 4685 6584 8578 2075
Size: 1124 1486 1899 1994 5060
Fragments arranged by size:
5060 1994 1899 1486 1124
KpnI G_GTAC'C
Cuts at: 8888 8888
Size: 11563
MfeI C'AATT_G
Cuts at: 9162 9162
Size: 11563
MluI A'CGCG_T
Cuts at: 2559 11439 2559
Size: 8880 2683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 6201 7035 7938 8107 8592 10677 10861
Size: 1323 3573 834 903 169 485 2085 184
Cuts at: 10861 1305
Size: 2007
Fragments arranged by size:
3573 2085 2007 1323 903 834 485 184
169
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2910 3112 3347 3371 6794
Size: 54 1425 343 1078 202 235 24 3423
Cuts at: 6794 10
Size: 4779
Fragments arranged by size:
4779 3423 1425 1078 343 235 202 54
24
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 3077 3215 4217 4397 4493 7400
Size: 648 1032 879 138 1002 180 96 2907
Cuts at: 7400 8075 8603 8702 9521 9880 10039 518
Size: 675 528 99 819 359 159 2042
Fragments arranged by size:
2907 2042 1032 1002 879 819 675 648
528 359 180 159 138 99 96
MspA1I CmG'CkG
Cuts at: 1606 3721 3997 4072 4249 4420 4932 4945 5845
Size: 2115 276 75 177 171 512 13 900
Cuts at: 5845 6355 6774 7473 8484 8679 8715 8854 8979
Size: 510 419 699 1011 195 36 139 125
Cuts at: 8979 9656 10804 11049 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 2115 1148 1011 900 699 677 512
510 419 276 245 195 177 171 139
125 75 36 13
NaeI GCC'GGC
Cuts at: 6031 6031
Size: 11563
NarI GG'CG_CC
Cuts at: 2076 3200 4686 6585 8579 2076
Size: 1124 1486 1899 1994 5060
Fragments arranged by size:
5060 1994 1899 1486 1124
NcoI C'CATG_G
Cuts at: 513 1065 3462 3936 4488 6883 513
Size: 552 2397 474 552 2395 5193
Fragments arranged by size:
5193 2397 2395 552 552 474
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11563
NgoMIV G'CCGG_C
Cuts at: 6029 6029
Size: 11563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11563
NotI GC'GGCC_GC
Cuts at: 3282 9010 3282
Size: 5728 5835
NsiI A_TGCA'T
Cuts at: 2205 2205
Size: 11563
NspI r_CATG'y
Cuts at: 1132 1229 2203 2668 3154 3337 5077 6223 8701
Size: 97 974 465 486 183 1740 1146 2478
Cuts at: 8701 8806 8921 10466 1132
Size: 105 115 1545 2229
Fragments arranged by size:
2478 2229 1740 1545 1146 974 486 465
183 115 105 97
PasI CC'CwG_GG
Cuts at: 1348 5785 1348
Size: 4437 7126
PciI A'CATG_T
Cuts at: 3333 6219 10462 3333
Size: 2886 4243 4434
Fragments arranged by size:
4434 4243 2886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 3367 4741 1108
Size: 1374 885 1374 7930
Fragments arranged by size:
7930 1374 1374 885
PfoI T'CCnGG_A
Cuts at: 3057 4287 5925 5982 6615 6867 8137 8814 3057
Size: 1230 1638 57 633 252 1270 677 5806
Fragments arranged by size:
5806 1638 1270 1230 677 633 252 57
PmeI GTTT'AAAC
Cuts at: 8867 8867
Size: 11563
PmlI CAC'GTG
Cuts at: 2722 3601 3634 5483 2722
Size: 879 33 1849 8802
Fragments arranged by size:
8802 1849 879 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 8417 8449 9632 9664 11145 11177 3557
Size: 32 4828 32 1183 32 1481 32 3943
Fragments arranged by size:
4828 3943 1481 1183 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5734 6163 6865 8656 5734
Size: 429 702 1791 8641
Fragments arranged by size:
8641 1791 702 429
PshAI GACnn'nnGTC
Cuts at: 1613 3620 7110 1613
Size: 2007 3490 6066
Fragments arranged by size:
6066 3490 2007
PsiI TTA'TAA
Cuts at: 9133 9133
Size: 11563
PspOMI G'GGCC_C
Cuts at: 4808 6312 6628 6746 4808
Size: 1504 316 118 9625
Fragments arranged by size:
9625 1504 316 118
PspXI vC'TCGA_Gb
Cuts at: 3644 3644
Size: 11563
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4862 4894 4862
Size: 32 11531
PstI C_TGCA'G
Cuts at: 830 2592 3720 3927 4419 4944 5460 5850 6701
Size: 1762 1128 207 492 525 516 390 851
Cuts at: 6701 7557 8655 8714 8841 8915 830
Size: 856 1098 59 127 74 3478
Fragments arranged by size:
3478 1762 1128 1098 856 851 525 516
492 390 207 127 74 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11563
PvuII CAG'CTG
Cuts at: 4072 4420 4945 5845 6355 6774 8484 8715 8854
Size: 348 525 900 510 419 1710 231 139
Cuts at: 8854 4072
Size: 6781
Fragments arranged by size:
6781 1710 900 525 510 419 348 231
139
SacI G_AGCT'C
Cuts at: 4908 5100 5354 5562 6639 8181 8253 4908
Size: 192 254 208 1077 1542 72 8218
Fragments arranged by size:
8218 1542 1077 254 208 192 72
SacII CC_GC'GG
Cuts at: 4933 8680 4933
Size: 3747 7816
SalI G'TCGA_C
Cuts at: 1936 8905 1936
Size: 6969 4594
SapI GCTCTTCn'nnn_
Cuts at: 4218 5359 5567 6506 7385 7553 4218
Size: 1141 208 939 879 168 8228
Fragments arranged by size:
8228 1141 939 879 208 168
SbfI CC_TGCA'GG
Cuts at: 8655 8915 8655
Size: 260 11303
ScaI AGT'ACT
Cuts at: 9811 11523 9811
Size: 1712 9851
SexAI A'CCwGG_T
Cuts at: 7452 8364 8658 7452
Size: 912 294 10357
Fragments arranged by size:
10357 912 294
SfcI C'TryA_G
Cuts at: 826 1045 2588 3716 3923 4415 4940 5019 5456
Size: 219 1543 1128 207 492 525 79 437
Cuts at: 5456 5846 6697 7068 7553 8624 8651 8710 8837
Size: 390 851 371 485 1071 27 59 127
Cuts at: 8837 8911 10046 10727 10918 826
Size: 74 1135 681 191 1471
Fragments arranged by size:
1543 1471 1135 1128 1071 851 681 525
492 485 437 390 371 219 207 191
127 79 74 59 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 3290 3290
Size: 11563
SfoI GGC'GCC
Cuts at: 2077 3201 4687 6586 8580 2077
Size: 1124 1486 1899 1994 5060
Fragments arranged by size:
5060 1994 1899 1486 1124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11563
SmaI CCC'GGG
Cuts at: 1872 3128 5917 8890 1872
Size: 1256 2789 2973 4545
Fragments arranged by size:
4545 2973 2789 1256
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 2852 3275 3644 3749 4907
Size: 197 926 156 753 423 369 105 1158
Cuts at: 4907 4985 5345 6398 9667 10568 10830 820
Size: 78 360 1053 3269 901 262 1553
Fragments arranged by size:
3269 1553 1158 1053 926 901 753 423
369 360 262 197 156 105 78
SnaBI TAC'GTA
Cuts at: 493 9354 493
Size: 8861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11563
SphI G_CATG'C
Cuts at: 1132 3154 8701 8921 1132
Size: 2022 5547 220 3774
Fragments arranged by size:
5547 3774 2022 220
SrfI GCCC'GGGC
Cuts at: 3128 3128
Size: 11563
SspI AAT'ATT
Cuts at: 5 52 8775 9487 5
Size: 47 8723 712 2081
Fragments arranged by size:
8723 2081 712 47
StuI AGG'CCT
Cuts at: 1517 3512 3530 4979 5603 6941 7295 1517
Size: 1995 18 1449 624 1338 354 5785
Fragments arranged by size:
5785 1995 1449 1338 624 354 18
StyI C'CwwG_G
Cuts at: 513 1065 3288 3462 3936 4215 4488 4839 5025
Size: 552 2223 174 474 279 273 351 186
Cuts at: 5025 6580 6883 9002 513
Size: 1555 303 2119 3074
Fragments arranged by size:
3074 2223 2119 1555 552 474 351 303
279 273 186 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3223 3444 4550 5685 7076 7399 7439
Size: 1255 1643 221 1106 1135 1391 323 40
Cuts at: 7439 7470 8762 9590 9607 9760 9945 10390 325
Size: 31 1292 828 17 153 185 445 1498
Fragments arranged by size:
1643 1498 1391 1292 1255 1135 1106 828
445 323 221 185 153 40 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3192 3411 5055
Size: 274 80 33 51 1443 1215 219 1644
Cuts at: 5055 6591 7625 7668 8706 9809 11521 96
Size: 1536 1034 43 1038 1103 1712 138
Fragments arranged by size:
1712 1644 1536 1443 1215 1103 1038 1034
274 219 138 80 51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3320 3352 3442 3474 5978 6010 11523 11555 3320
Size: 32 90 32 2504 32 5513 32 3328
Fragments arranged by size:
5513 3328 2504 90 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 8660 1316
Size: 7344 4219
XbaI T'CTAG_A
Cuts at: 8899 8899
Size: 11563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 3460 7180 7660 2416
Size: 1044 3720 480 6319
Fragments arranged by size:
6319 3720 1044 480
XhoI C'TCGA_G
Cuts at: 1943 3644 1943
Size: 1701 9862
XmaI C'CCGG_G
Cuts at: 1870 3126 5915 8888 1870
Size: 1256 2789 2973 4545
Fragments arranged by size:
4545 2973 2789 1256
XmnI GAAnn'nnTTC
Cuts at: 4445 7547 7743 7789 7988 9692 4445
Size: 3102 196 46 199 1704 6316
Fragments arranged by size:
6316 3102 1704 199 196 46
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9371 276
Size: 53 83 186 936 7837 2468
Fragments arranged by size:
7837 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FspAI NruI PacI
RsrII SanDI SwaI