Restriction map for
pFN21AB5621
(Circular) (Six-base) MAPSORT of: pFN21AB5621.seq Check: 2649 from: 1 to: 8773
pFN21AB5621 8773 bp
With 182 enzymes: SgfI *
March 28, 2012 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4220 4220
Size: 8773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5268 6583 278
Size: 53 83 186 936 3732 1315 2468
Fragments arranged by size:
3732 2468 1315 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6116 1937
Size: 4179 4594
Acc65I G'GTAC_C
Cuts at: 6094 6094
Size: 8773
AclI AA'CG_TT
Cuts at: 1563 6900 7273 1563
Size: 5337 373 3063
Fragments arranged by size:
5337 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4415 4526 5001 5913 6836 8220 8488 1632
Size: 2783 111 475 912 923 1384 268 1917
Fragments arranged by size:
2783 1917 1384 923 912 475 268 111
AflII C'TTAA_G
Cuts at: 820 1017 3890 820
Size: 197 2873 5703
Fragments arranged by size:
5703 2873 197
AflIII A'CryG_T
Cuts at: 1203 2478 3335 7672 8649 1203
Size: 1275 857 4337 977 1327
Fragments arranged by size:
4337 1327 1275 977 857
AgeI A'CCGG_T
Cuts at: 6011 7604 6011
Size: 1593 7180
AhdI GACnn_n'nnGTC
Cuts at: 3651 5269 7502 3651
Size: 1618 2233 4922
Fragments arranged by size:
4922 2233 1618
AleI CACnn'nnGTG
Cuts at: 1166 4319 5954 1166
Size: 3153 1635 3985
Fragments arranged by size:
3985 3153 1635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 5520 1184
Size: 852 3484 4437
Fragments arranged by size:
4437 3484 852
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4650 4682 4650
Size: 32 8741
AlwNI CAG_nnn'CTG
Cuts at: 3775 5674 6516 8088 3775
Size: 1899 842 1572 4460
Fragments arranged by size:
4460 1899 1572 842
ApaI G_GGCC'C
Cuts at: 5386 5386
Size: 8773
ApaLI G'TGCA_C
Cuts at: 3715 6830 7986 3715
Size: 3115 1156 4502
Fragments arranged by size:
4502 3115 1156
ApoI r'AATT_y
Cuts at: 1493 2532 2864 4487 4754 4763 4805 5345 5636
Size: 1039 332 1623 267 9 42 540 291
Cuts at: 5636 6082 6308 6460 8569 1493
Size: 446 226 152 2109 1697
Fragments arranged by size:
2109 1697 1623 1039 540 446 332 291
267 226 152 42 9
AseI AT'TA_AT
Cuts at: 160 1032 2616 7327 160
Size: 872 1584 4711 1606
Fragments arranged by size:
4711 1606 1584 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8773
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2456 5154 6043 6098 1870
Size: 73 112 401 2698 889 55 4545
Fragments arranged by size:
4545 2698 889 401 112 73 55
BamHI G'GATC_C
Cuts at: 4423 6103 6470 4423
Size: 1680 367 6726
Fragments arranged by size:
6726 1680 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3317 4000 4271 4532 4712
Size: 325 217 627 1530 683 271 261 180
Cuts at: 4712 5860 6094 7549 618
Size: 1148 234 1455 1842
Fragments arranged by size:
1842 1530 1455 1148 683 627 325 271
261 234 217 180
BanII G_rGCy'C
Cuts at: 1391 3414 4648 4919 5386 5445 6062 6092 1391
Size: 2023 1234 271 467 59 617 30 4072
Fragments arranged by size:
4072 2023 1234 617 467 271 59 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 3953 4137 4403 5829 928
Size: 971 691 1363 184 266 1426 3872
Fragments arranged by size:
3872 1426 1363 971 691 266 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6964 6998 1903
Size: 34 50 34 4943 34 3678
Fragments arranged by size:
4943 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3817 5087 6665 7875 3817
Size: 1270 1578 1210 4715
Fragments arranged by size:
4715 1578 1270 1210
BclI T'GATC_A
Cuts at: 1548 2577 3879 1548
Size: 1029 1302 6442
Fragments arranged by size:
6442 1302 1029
BfrBI ATG'CAT
Cuts at: 3899 3899
Size: 8773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 7384 136
Size: 107 122 71 631 605 3060 2652 1525
Fragments arranged by size:
3060 2652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2369 3728 4984 8768 2369
Size: 1359 1256 3784 2374
Fragments arranged by size:
3784 2374 1359 1256
BlpI GC'TnA_GC
Cuts at: 2105 4918 5508 6190 2105
Size: 2813 590 682 4688
Fragments arranged by size:
4688 2813 682 590
Bme1580I G_kGCm'C
Cuts at: 1790 3719 4276 4834 5386 5865 6834 7990 1790
Size: 1929 557 558 552 479 969 1156 2573
Fragments arranged by size:
2573 1929 1156 969 558 557 552 479
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 2836 5439
Size: 416 53 83 399 24 1374 45 2603
Cuts at: 5439 6504 7462 442
Size: 1065 958 1753
Fragments arranged by size:
2603 1753 1374 1065 958 416 399 83
53 45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 4754 6081 2712
Size: 2042 1327 5404
Fragments arranged by size:
5404 2042 1327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 4043 5947 6017 7433 1239
Size: 1080 1724 1904 70 1416 2579
Fragments arranged by size:
2579 1904 1724 1416 1080 70
Bpu10I CC'TnA_GC
Cuts at: 5043 5043
Size: 8773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3137 3921 4646 6898 7763 8061 3137
Size: 784 725 2252 865 298 3849
Fragments arranged by size:
3849 2252 865 784 725 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 2823 3247 4112 4491 5446 7436
Size: 617 546 778 424 865 379 955 1990
Cuts at: 7436 882
Size: 2219
Fragments arranged by size:
2219 1990 955 865 778 617 546 424
379
BsaAI yAC'GTr
Cuts at: 493 1204 3643 6564 493
Size: 711 2439 2921 2702
Fragments arranged by size:
2921 2702 2439 711
BsaBI GATnn'nnATC
Cuts at: 8767 8767
Size: 8773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5265 6580 6962 7597
Size: 53 83 186 936 3732 1315 382 635
Cuts at: 7597 275
Size: 1451
Fragments arranged by size:
3732 1451 1315 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4161 5924 6011 7206 7604 7878 8025 8436 4161
Size: 1763 87 1195 398 274 147 411 4498
Fragments arranged by size:
4498 1763 1195 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3383 3413 3612 3642 3689
Size: 30 165 30 1980 30 199 30 47
Cuts at: 3689 3719 3909 3939 4931 4961 1178
Size: 30 190 30 992 30 4990
Fragments arranged by size:
4990 1980 992 199 190 165 47 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3633 4804 4955 5627 5630 6054 1186
Size: 2447 1171 151 672 3 424 3905
Fragments arranged by size:
3905 2447 1171 672 424 151 3
BseYI C'CCAG_C
Cuts at: 2567 3413 5864 7976 8472 2567
Size: 846 2451 2112 496 2868
Fragments arranged by size:
2868 2451 2112 846 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 2946 1163
Size: 573 524 686 6990
Fragments arranged by size:
6990 686 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6223 6984 8012 1540
Size: 275 186 4222 761 1028 2301
Fragments arranged by size:
4222 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2876 3719 4648 4919 6062 6834 6919 7990 2876
Size: 843 929 271 1143 772 85 1071 3659
Fragments arranged by size:
3659 1143 1071 929 843 772 271 85
BsmI GAATG_Cn'
Cuts at: 3847 3901 6284 6377 3847
Size: 54 2383 93 6243
Fragments arranged by size:
6243 2383 93 54
BsmBI CGTCTCn'nnnn_
Cuts at: 2700 2700
Size: 8773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2876 3414 3719 4276 4648 4834 4919
Size: 399 1086 538 305 557 372 186 85
Cuts at: 4919 5386 5445 5865 6062 6092 6834 6919 7990
Size: 467 59 420 197 30 742 85 1071
Cuts at: 7990 1391
Size: 2174
Fragments arranged by size:
2174 1086 1071 742 557 538 467 420
399 372 305 197 186 85 85 59
30
BspEI T'CCGG_A
Cuts at: 5924 8436 5924
Size: 2512 6261
BspHI T'CATG_A
Cuts at: 6662 8392 6662
Size: 1730 7043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4220 6128 844
Size: 3376 1908 3489
Fragments arranged by size:
3489 3376 1908
BsrBI CCG'CTC
Cuts at: 1394 1823 6660 1394
Size: 429 4837 3507
Fragments arranged by size:
4837 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3961 7268 7442 65
Size: 1183 2713 3307 174 1396
Fragments arranged by size:
3307 2713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4500 5697 6011 7417 7604 1700
Size: 110 2690 1197 314 1406 187 2869
Fragments arranged by size:
2869 2690 1406 1197 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 2412 2643 2741 2916 3585 5247 96
Size: 2316 231 98 175 669 1662 3622
Fragments arranged by size:
3622 2316 1662 669 231 175 98
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8773
BssSI C'ACGA_G
Cuts at: 6833 7845 6833
Size: 1012 7761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8773
BstBI TT'CG_AA
Cuts at: 2798 7577 2798
Size: 4779 3994
BstEII G'GTnAC_C
Cuts at: 1184 3071 4682 1184
Size: 1887 1611 5275
Fragments arranged by size:
5275 1887 1611
BstXI CCAn_nnnn'nTGG
Cuts at: 4420 4420
Size: 8773
BstYI r'GATC_y
Cuts at: 1972 2369 3728 4423 4665 4876 4984 5170 6103
Size: 397 1359 695 242 211 108 186 933
Cuts at: 6103 6470 6855 6872 8324 8410 8422 8768 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1359 933 695 397 385 367
346 242 211 186 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4787 5305 4787
Size: 518 8255
BtgI C'CryG_G
Cuts at: 513 1065 3030 4528 513
Size: 552 1965 1498 4758
Fragments arranged by size:
4758 1965 1498 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2259 5049 487
Size: 683 56 1033 2790 4211
Fragments arranged by size:
4211 2790 1033 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 3717 4231 6289 7083 7103 2218
Size: 1499 514 2058 794 20 3888
Fragments arranged by size:
3888 2058 1499 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3362 3397 3768 3803 4854 4889 529
Size: 35 2798 35 371 35 1051 35 4413
Fragments arranged by size:
4413 2798 1051 371 35 35 35 35
DraI TTT'AAA
Cuts at: 4108 5985 6077 4108
Size: 1877 92 6804
Fragments arranged by size:
6804 1877 92
DraIII CAC_nnn'GTG
Cuts at: 2489 4634 2489
Size: 2145 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7780 809
Size: 507 6464 1802
Fragments arranged by size:
6464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 4525 4540
Size: 54 1425 50 275 18 195 2500 15
Cuts at: 4540 6220 7109 8738 8
Size: 1680 889 1629 43
Fragments arranged by size:
2500 1680 1629 1425 889 275 195 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 6220 1537
Size: 275 4408 4090
Fragments arranged by size:
4408 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2258 3390 4962 5559 5799 6703 1353
Size: 801 104 1132 1572 597 240 904 3423
Fragments arranged by size:
3423 1572 1132 904 801 597 240 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7360 7734 7880 387
Size: 1064 138 5771 374 146 1280
Fragments arranged by size:
5771 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4646 4917 6060 4646
Size: 271 1143 7359
Fragments arranged by size:
7359 1143 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 4043 4415 4526 5001 5913 5947
Size: 393 687 1724 372 111 475 912 34
Cuts at: 5947 6017 6836 7433 8220 8488 1239
Size: 70 819 597 787 268 1524
Fragments arranged by size:
1724 1524 912 819 787 687 597 475
393 372 268 111 70 34
EcoO109I rG'GnC_Cy
Cuts at: 3653 5086 5382 5383 3653
Size: 1433 296 1 7043
Fragments arranged by size:
7043 1433 296 1
EcoRI G'AATT_C
Cuts at: 4487 4754 6082 6460 4487
Size: 267 1328 378 6800
Fragments arranged by size:
6800 1328 378 267
EcoRV GAT'ATC
Cuts at: 5544 5728 5930 5544
Size: 184 202 8387
Fragments arranged by size:
8387 202 184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2141 2572 2749 4306 5996 2141
Size: 431 177 1557 1690 4918
Fragments arranged by size:
4918 1690 1557 431 177
FspI TGC'GCA
Cuts at: 7279 7279
Size: 8773
HaeII r_GCGC'y
Cuts at: 1678 4891 7920 1678
Size: 3213 3029 2531
Fragments arranged by size:
3213 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2354 2386 2437
Size: 32 135 32 749 32 1 32 51
Cuts at: 2437 2469 3637 3669 3689 3721 3909 3941 4931
Size: 32 1168 32 20 32 188 32 990
Cuts at: 4931 4963 5255 5287 7414 7446 7488 7520 1373
Size: 32 292 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 1168 990 749 292 188 135
51 42 32 32 32 32 32 32
32 32 32 32 32 32 20 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3604 4702 6117 6363 669
Size: 988 281 1666 1098 1415 246 3079
Fragments arranged by size:
3079 1666 1415 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2726 3758 748
Size: 1978 1032 5763
Fragments arranged by size:
5763 1978 1032
HpaI GTT'AAC
Cuts at: 6363 6363
Size: 8773
KpnI G_GTAC'C
Cuts at: 6098 6098
Size: 8773
MfeI C'AATT_G
Cuts at: 6372 6372
Size: 8773
MluI A'CGCG_T
Cuts at: 8649 8649
Size: 8773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2698 3369 4840 7887 8071 1305
Size: 1393 671 1471 3047 184 2007
Fragments arranged by size:
3047 2007 1471 1393 671 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4542 10
Size: 54 1425 343 2710 4241
Fragments arranged by size:
4241 2710 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4319 4418 4692 5937 5954 6731 7090
Size: 648 3153 99 274 1245 17 777 359
Cuts at: 7090 7249 518
Size: 159 2042
Fragments arranged by size:
3153 2042 1245 777 648 359 274 159
99 17
MspA1I CmG'CkG
Cuts at: 1606 6189 6866 8014 8259 1606
Size: 4583 677 1148 245 2120
Fragments arranged by size:
4583 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 5699 5699
Size: 8773
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8773
NgoMIV G'CCGG_C
Cuts at: 5697 5697
Size: 8773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8773
NotI GC'GGCC_GC
Cuts at: 6220 6220
Size: 8773
NruI TCG'CGA
Cuts at: 5068 5068
Size: 8773
NsiI A_TGCA'T
Cuts at: 3901 3901
Size: 8773
NspI r_CATG'y
Cuts at: 1132 1229 3149 3339 3682 4224 6131 7676 1132
Size: 97 1920 190 343 542 1907 1545 2229
Fragments arranged by size:
2229 1920 1907 1545 542 343 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8773
PciI A'CATG_T
Cuts at: 3335 7672 3335
Size: 4337 4436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4482 5324 5849 1108
Size: 3374 842 525 4032
Fragments arranged by size:
4032 3374 842 525
PmeI GTTT'AAAC
Cuts at: 6077 6077
Size: 8773
PmlI CAC'GTG
Cuts at: 3643 3643
Size: 8773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6842 6874 8355 8387 6842
Size: 32 1481 32 7228
Fragments arranged by size:
7228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3653 5086 3653
Size: 1433 7340
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8773
PsiI TTA'TAA
Cuts at: 5166 6343 5166
Size: 1177 7596
PspOMI G'GGCC_C
Cuts at: 5382 5382
Size: 8773
PstI C_TGCA'G
Cuts at: 830 3724 5679 6125 830
Size: 2894 1955 446 3478
Fragments arranged by size:
3478 2894 1955 446
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8773
RsrII CG'GwC_CG
Cuts at: 4365 4365
Size: 8773
SacI G_AGCT'C
Cuts at: 4648 4919 6062 4648
Size: 271 1143 7359
Fragments arranged by size:
7359 1143 271
SalI G'TCGA_C
Cuts at: 1936 6115 1936
Size: 4179 4594
SapI GCTCTTCn'nnn_
Cuts at: 5799 5799
Size: 8773
SbfI CC_TGCA'GG
Cuts at: 6125 6125
Size: 8773
ScaI AGT'ACT
Cuts at: 7021 8733 7021
Size: 1712 7061
SfcI C'TryA_G
Cuts at: 826 1045 2883 3720 5675 6121 7256 7937 8128
Size: 219 1838 837 1955 446 1135 681 191
Cuts at: 8128 826
Size: 1471
Fragments arranged by size:
1955 1838 1471 1135 837 681 446 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8773
SmaI CCC'GGG
Cuts at: 1872 2057 5156 6100 1872
Size: 185 3099 944 4545
Fragments arranged by size:
4545 3099 944 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 3890 3936 4661 6877 7778
Size: 197 926 1173 774 46 725 2216 901
Cuts at: 7778 8040 820
Size: 262 1553
Fragments arranged by size:
2216 1553 1173 926 901 774 725 262
197 46
SnaBI TAC'GTA
Cuts at: 493 6564 493
Size: 6071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8773
SphI G_CATG'C
Cuts at: 1132 4224 6131 1132
Size: 3092 1907 3774
Fragments arranged by size:
3774 3092 1907
SspI AAT'ATT
Cuts at: 5 52 5298 6697 5
Size: 47 5246 1399 2081
Fragments arranged by size:
5246 2081 1399 47
StuI AGG'CCT
Cuts at: 1517 4203 1517
Size: 2686 6087
StyI C'CwwG_G
Cuts at: 513 1065 2908 3067 3913 5007 6212 513
Size: 552 1843 159 846 1094 1205 3074
Fragments arranged by size:
3074 1843 1205 1094 846 552 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2329 4018 6800 6817 6970 7155 7600
Size: 1255 749 1689 2782 17 153 185 445
Cuts at: 7600 325
Size: 1498
Fragments arranged by size:
2782 1689 1498 1255 749 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 2643 2741
Size: 274 80 33 51 1443 435 231 98
Cuts at: 2741 2916 3148 3585 5247 5958 7019 8731 96
Size: 175 232 437 1662 711 1061 1712 138
Fragments arranged by size:
1712 1662 1443 1061 711 437 435 274
232 231 175 138 98 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 3612 3644 5088 5120 8733 8765 3383
Size: 32 197 32 1444 32 3613 32 3391
Fragments arranged by size:
3613 3391 1444 197 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3923 1316
Size: 2607 6166
XbaI T'CTAG_A
Cuts at: 6109 6109
Size: 8773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3090 4098 5780 3090
Size: 1008 1682 6083
Fragments arranged by size:
6083 1682 1008
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8773
XmaI C'CCGG_G
Cuts at: 1870 2055 5154 6098 1870
Size: 185 3099 944 4545
Fragments arranged by size:
4545 3099 944 185
XmnI GAAnn'nnTTC
Cuts at: 6902 6902
Size: 8773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5266 6581 276
Size: 53 83 186 936 3732 1315 2468
Fragments arranged by size:
3732 2468 1315 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
RsrII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I
EcoNI FseI FspAI KasI NarI PacI PfoI PspXI
PsrI PvuII SacII SanDI SexAI SfiI SfoI SrfI
SwaI