Restriction map for
pFN21AB5617
(Circular) (Six-base) MAPSORT of: pFN21AB5617.seq Check: 4395 from: 1 to: 8929
pFN21AB5617 8929 bp
With 182 enzymes: SgfI *
April 1, 2016 16:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6739 278
Size: 53 83 186 936 5203 2468
Fragments arranged by size:
5203 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2370 5061 6272 1937
Size: 433 2691 1211 4594
Fragments arranged by size:
4594 2691 1211 433
Acc65I G'GTAC_C
Cuts at: 6250 6250
Size: 8929
AclI AA'CG_TT
Cuts at: 1563 7056 7429 1563
Size: 5493 373 3063
Fragments arranged by size:
5493 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2939 4195 4680 4746 6992 8376 8644
Size: 590 717 1256 485 66 2246 1384 268
Cuts at: 8644 1632
Size: 1917
Fragments arranged by size:
2246 1917 1384 1256 717 590 485 268
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8732
AflIII A'CryG_T
Cuts at: 1203 2434 4091 5148 5742 7828 8805 1203
Size: 1231 1657 1057 594 2086 977 1327
Fragments arranged by size:
2086 1657 1327 1231 1057 977 594
AgeI A'CCGG_T
Cuts at: 2574 7760 2574
Size: 5186 3743
AhdI GACnn_n'nnGTC
Cuts at: 2467 7658 2467
Size: 5191 3738
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 1184
Size: 873 8056
AlwNI CAG_nnn'CTG
Cuts at: 2847 3062 6672 8244 2847
Size: 215 3610 1572 3532
Fragments arranged by size:
3610 3532 1572 215
ApaI G_GGCC'C
Cuts at: 2986 5277 2986
Size: 2291 6638
ApaLI G'TGCA_C
Cuts at: 3720 6986 8142 3720
Size: 3266 1156 4507
Fragments arranged by size:
4507 3266 1156
ApoI r'AATT_y
Cuts at: 1493 2608 3217 3436 3788 4060 4242 4360 4846
Size: 1115 609 219 352 272 182 118 486
Cuts at: 4846 5370 5557 5702 6238 6464 6616 8725 1493
Size: 524 187 145 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 609 536 524 486 352
272 226 219 187 182 152 145 118
AseI AT'TA_AT
Cuts at: 160 1032 7483 160
Size: 872 6451 1606
Fragments arranged by size:
6451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8929
AvaI C'yCGr_G
Cuts at: 1870 1943 4375 6254 1870
Size: 73 2432 1879 4545
Fragments arranged by size:
4545 2432 1879 73
BamHI G'GATC_C
Cuts at: 3970 4533 6259 6626 3970
Size: 563 1726 367 6273
Fragments arranged by size:
6273 1726 563 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3624 6018 6250 7705
Size: 325 217 627 411 1426 2394 232 1455
Cuts at: 7705 618
Size: 1842
Fragments arranged by size:
2394 1842 1455 1426 627 411 325 232
217
BanII G_rGCy'C
Cuts at: 1391 2511 2986 5277 5902 6248 1391
Size: 1120 475 2291 625 346 4072
Fragments arranged by size:
4072 2291 1120 625 475 346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 5606 5965 928
Size: 971 339 3368 359 3892
Fragments arranged by size:
3892 3368 971 359 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7120 7154 1903
Size: 34 5183 34 3678
Fragments arranged by size:
5183 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5145 6162 6821 8031 5145
Size: 1017 659 1210 6043
Fragments arranged by size:
6043 1210 1017 659
BclI T'GATC_A
Cuts at: 1548 2386 1548
Size: 838 8091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 7540 136
Size: 107 122 71 631 605 337 5531 1525
Fragments arranged by size:
5531 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3681 4945 5548 5596 8924 3681
Size: 1264 603 48 3328 3686
Fragments arranged by size:
3686 3328 1264 603 48
BlpI GC'TnA_GC
Cuts at: 6346 6346
Size: 8929
Bme1580I G_kGCm'C
Cuts at: 1790 2986 3724 5017 5277 6990 8146 1790
Size: 1196 738 1293 260 1713 1156 2573
Fragments arranged by size:
2573 1713 1293 1196 1156 738 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 3531 5271
Size: 416 53 83 399 24 854 1260 1740
Cuts at: 5271 6660 7618 442
Size: 1389 958 1753
Fragments arranged by size:
1753 1740 1389 1260 958 854 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2912 3146 3450 7589 1239
Size: 1673 234 304 4139 2579
Fragments arranged by size:
4139 2579 1673 304 234
Bpu10I CC'TnA_GC
Cuts at: 3659 3659
Size: 8929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7054 7919 8217 7054
Size: 865 298 7766
Fragments arranged by size:
7766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3342 5517 7592 882
Size: 617 1843 2175 2075 2219
Fragments arranged by size:
2219 2175 2075 1843 617
BsaAI yAC'GTr
Cuts at: 493 1204 6720 493
Size: 711 5516 2702
Fragments arranged by size:
5516 2702 711
BsaBI GATnn'nnATC
Cuts at: 3249 8923 3249
Size: 5674 3255
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6736 7118 7753 275
Size: 53 83 186 936 5203 382 635 1451
Fragments arranged by size:
5203 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2574 7362 7760 8034 8181 8592 2574
Size: 4788 398 274 147 411 2911
Fragments arranged by size:
4788 2911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 1178
Size: 30 165 30 1135 30 7539
Fragments arranged by size:
7539 1135 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 4980 5864 5910 1186
Size: 894 2900 884 46 4205
Fragments arranged by size:
4205 2900 894 884 46
BseYI C'CCAG_C
Cuts at: 2510 2985 3759 4138 8132 8628 2510
Size: 475 774 379 3994 496 2811
Fragments arranged by size:
3994 2811 774 496 475 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 3817 5150 5276 1163
Size: 573 345 1736 1333 126 4816
Fragments arranged by size:
4816 1736 1333 573 345 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3647 6379 7140 8168 1540
Size: 275 186 1646 2732 761 1028 2301
Fragments arranged by size:
2732 2301 1646 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3192 3724 3857 4334 5230 5874 6102 6990
Size: 1146 532 133 477 896 644 228 888
Cuts at: 6990 7075 8146 2046
Size: 85 1071 2829
Fragments arranged by size:
2829 1146 1071 896 888 644 532 477
228 133 85
BsmI GAATG_Cn'
Cuts at: 2638 2869 3095 3659 6440 6533 2638
Size: 231 226 564 2781 93 5034
Fragments arranged by size:
5034 2781 564 231 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2511 2986 3192 3724 3857 4334
Size: 399 256 465 475 206 532 133 477
Cuts at: 4334 5017 5230 5277 5874 5902 6102 6248 6990
Size: 683 213 47 597 28 200 146 742
Cuts at: 6990 7075 8146 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 597 532 477 475
465 399 256 213 206 200 146 133
85 47 28
BspEI T'CCGG_A
Cuts at: 8592 8592
Size: 8929
BspHI T'CATG_A
Cuts at: 3184 5448 6818 8548 3184
Size: 2264 1370 1730 3565
Fragments arranged by size:
3565 2264 1730 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 3385 4764 6284 844
Size: 1588 953 1379 1520 3489
Fragments arranged by size:
3489 1588 1520 1379 953
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 6816 1394
Size: 429 1840 3153 3507
Fragments arranged by size:
3507 3153 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2077 2633 4624 7424 7598 65
Size: 1183 829 556 1991 2800 174 1396
Fragments arranged by size:
2800 1991 1396 1183 829 556 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 7573 7760 1700
Size: 110 764 4999 187 2869
Fragments arranged by size:
4999 2869 764 187 110
BsrGI T'GTAC_A
Cuts at: 96 4564 5767 96
Size: 4468 1203 3258
Fragments arranged by size:
4468 3258 1203
BssHII G'CGCG_C
Cuts at: 1925 3236 1925
Size: 1311 7618
BssSI C'ACGA_G
Cuts at: 2039 6989 8001 2039
Size: 4950 1012 2967
Fragments arranged by size:
4950 2967 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3588 794
Size: 2794 6135
BstBI TT'CG_AA
Cuts at: 5554 5566 7733 5554
Size: 12 2167 6750
Fragments arranged by size:
6750 2167 12
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8929
BstXI CCAn_nnnn'nTGG
Cuts at: 5802 5802
Size: 8929
BstYI r'GATC_y
Cuts at: 1972 2924 3540 3681 3970 4225 4533 4945 5548
Size: 952 616 141 289 255 308 412 603
Cuts at: 5548 5596 6259 6626 7011 7028 8480 8566 8578
Size: 48 663 367 385 17 1452 86 12
Cuts at: 8578 8924 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 952 663 616 603 412 385
367 346 308 289 255 141 86 48
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2145 3934 513
Size: 552 1080 1789 5508
Fragments arranged by size:
5508 1789 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4418 487
Size: 683 56 3192 4998
Fragments arranged by size:
4998 3192 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 3190 4960 5259 6445 7239 7259 2479
Size: 711 1770 299 1186 794 20 4149
Fragments arranged by size:
4149 1770 1186 794 711 299 20
ClaI AT'CG_AT
Cuts at: 1554 3079 1554
Size: 1525 7404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8894
DraI TTT'AAA
Cuts at: 2223 3798 4101 6233 2223
Size: 1575 303 2132 4919
Fragments arranged by size:
4919 2132 1575 303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2698 5149 7936 809
Size: 507 1382 2451 2787 1802
Fragments arranged by size:
2787 2451 1802 1382 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3931 6376 7265
Size: 54 1425 50 275 18 2101 2445 889
Cuts at: 7265 8894 8
Size: 1629 43
Fragments arranged by size:
2445 2101 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6376 1537
Size: 275 4564 4090
Fragments arranged by size:
4564 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 5308 6859 1353
Size: 1146 2809 1551 3423
Fragments arranged by size:
3423 2809 1551 1146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7516 7890 8036 387
Size: 1064 138 5927 374 146 1280
Fragments arranged by size:
5927 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2912 2939 3146 3450 4195 4680
Size: 393 590 690 27 207 304 745 485
Cuts at: 4680 4746 6992 7589 8376 8644 1239
Size: 66 2246 597 787 268 1524
Fragments arranged by size:
2246 1524 787 745 690 597 590 485
393 304 268 207 66 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 8929
EcoO109I rG'GnC_Cy
Cuts at: 2011 2421 2982 2011
Size: 410 561 7958
Fragments arranged by size:
7958 561 410
EcoRI G'AATT_C
Cuts at: 4060 4360 6238 6616 4060
Size: 300 1878 378 6373
Fragments arranged by size:
6373 1878 378 300
EcoRV GAT'ATC
Cuts at: 2646 6069 2646
Size: 3423 5506
FspI TGC'GCA
Cuts at: 7435 7435
Size: 8929
HaeII r_GCGC'y
Cuts at: 1678 2541 8076 1678
Size: 863 5535 2531
Fragments arranged by size:
5535 2531 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2538 2570 3658
Size: 32 135 32 881 32 53 32 1088
Cuts at: 3658 3690 5566 5598 5843 5875 7570 7602 7644
Size: 32 1876 32 245 32 1695 32 42
Cuts at: 7644 7676 1373
Size: 32 2626
Fragments arranged by size:
2626 1876 1695 1088 881 245 135 53
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 2413 3115 3139 3286 4430
Size: 988 281 433 42 702 24 147 1144
Cuts at: 4430 6273 6519 669
Size: 1843 246 3079
Fragments arranged by size:
3079 1843 1144 988 702 433 281 246
147 42 24
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8929
HpaI GTT'AAC
Cuts at: 6519 6519
Size: 8929
KpnI G_GTAC'C
Cuts at: 6254 6254
Size: 8929
MfeI C'AATT_G
Cuts at: 4077 6528 4077
Size: 2451 6478
MluI A'CGCG_T
Cuts at: 8805 8805
Size: 8929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 4274 4669 5331 5609 8043 8227 1305
Size: 1146 1823 395 662 278 2434 184 2007
Fragments arranged by size:
2434 2007 1823 1146 662 395 278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3933 10
Size: 54 1425 343 2101 5006
Fragments arranged by size:
5006 2101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2354 2882 3871 4391 5298 5469
Size: 648 873 315 528 989 520 907 171
Cuts at: 5469 6887 7246 7405 518
Size: 1418 359 159 2042
Fragments arranged by size:
2042 1418 989 907 873 648 528 520
359 315 171 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2147 4483 5518 6345 7022 8170 8415
Size: 412 129 2336 1035 827 677 1148 245
Cuts at: 8415 1606
Size: 2120
Fragments arranged by size:
2336 2120 1148 1035 827 677 412 245
129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8377
NdeI CA'TA_TG
Cuts at: 387 3752 387
Size: 3365 5564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8929
NotI GC'GGCC_GC
Cuts at: 6376 6376
Size: 8929
NspI r_CATG'y
Cuts at: 1132 1229 2438 4095 4428 5152 5264 5746 6287
Size: 97 1209 1657 333 724 112 482 541
Cuts at: 6287 7832 1132
Size: 1545 2229
Fragments arranged by size:
2229 1657 1545 1209 724 541 482 333
112 97
PasI CC'CwG_GG
Cuts at: 1348 2542 1348
Size: 1194 7735
PciI A'CATG_T
Cuts at: 2434 4091 5148 5742 7828 2434
Size: 1657 1057 594 2086 3535
Fragments arranged by size:
3535 2086 1657 1057 594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3551 1108
Size: 2443 6486
PfoI T'CCnGG_A
Cuts at: 3444 6062 3444
Size: 2618 6311
PmeI GTTT'AAAC
Cuts at: 6233 6233
Size: 8929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6998 7030 8511 8543 6998
Size: 32 1481 32 7384
Fragments arranged by size:
7384 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2421 2421
Size: 8929
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8929
PsiI TTA'TAA
Cuts at: 6499 6499
Size: 8929
PspOMI G'GGCC_C
Cuts at: 2982 5273 2982
Size: 2291 6638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3247 3279 3247
Size: 32 8897
PstI C_TGCA'G
Cuts at: 830 2309 3566 3584 6281 830
Size: 1479 1257 18 2697 3478
Fragments arranged by size:
3478 2697 1479 1257 18
PvuI CG_AT'CG
Cuts at: 2001 3647 2001
Size: 1646 7283
PvuII CAG'CTG
Cuts at: 4483 4483
Size: 8929
SacII CC_GC'GG
Cuts at: 2148 2148
Size: 8929
SalI G'TCGA_C
Cuts at: 1936 2369 6271 1936
Size: 433 3902 4594
Fragments arranged by size:
4594 3902 433
SapI GCTCTTCn'nnn_
Cuts at: 2499 2499
Size: 8929
SbfI CC_TGCA'GG
Cuts at: 6281 6281
Size: 8929
ScaI AGT'ACT
Cuts at: 4270 7177 8889 4270
Size: 2907 1712 4310
Fragments arranged by size:
4310 2907 1712
SfcI C'TryA_G
Cuts at: 826 1045 2305 3562 3580 5034 5058 5761 6277
Size: 219 1260 1257 18 1454 24 703 516
Cuts at: 6277 7412 8093 8284 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1454 1260 1257 1135 703 681 516
219 191 24 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8929
SmaI CCC'GGG
Cuts at: 1872 6256 1872
Size: 4384 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7033 7934 8196 820
Size: 197 926 5090 901 262 1553
Fragments arranged by size:
5090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6720 493
Size: 6227 2702
SpeI A'CTAG_T
Cuts at: 152 5165 152
Size: 5013 3916
SphI G_CATG'C
Cuts at: 1132 6287 1132
Size: 5155 3774
SspI AAT'ATT
Cuts at: 5 52 6853 5
Size: 47 6801 2081
Fragments arranged by size:
6801 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8929
StyI C'CwwG_G
Cuts at: 513 1065 2055 2194 3817 4816 6368 513
Size: 552 990 139 1623 999 1552 3074
Fragments arranged by size:
3074 1623 1552 999 990 552 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 2295 5450 6956 6973 7126 7311
Size: 1255 590 125 3155 1506 17 153 185
Cuts at: 7311 7756 325
Size: 445 1498
Fragments arranged by size:
3155 1506 1498 1255 590 445 185 153
125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3953 4094
Size: 274 80 33 51 1443 276 1700 141
Cuts at: 4094 4268 4564 4627 4981 5767 7175 8887 96
Size: 174 296 63 354 786 1408 1712 138
Fragments arranged by size:
1712 1700 1443 1408 786 354 296 276
274 174 141 138 80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 3868 3900 8889 8921 2019
Size: 32 1817 32 4989 32 2027
Fragments arranged by size:
4989 2027 1817 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8929
XbaI T'CTAG_A
Cuts at: 2809 4064 6265 2809
Size: 1255 2201 5473
Fragments arranged by size:
5473 2201 1255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6213 6213
Size: 8929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8929
XmaI C'CCGG_G
Cuts at: 1870 6254 1870
Size: 4384 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4962 7058 2235
Size: 2727 2096 4106
Fragments arranged by size:
4106 2727 2096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6737 276
Size: 53 83 186 936 5203 2468
Fragments arranged by size:
5203 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI