Restriction map for
pFN21AB5616
(Circular) (Six-base) MAPSORT of: pFN21AB5616.seq Check: 6926 from: 1 to: 7069
pFN21AB5616 7069 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 2639 3209 3788 3899 2389
Size: 250 570 579 111 5559
Fragments arranged by size:
5559 579 570 250 111
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4879 278
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 4412 1937
Size: 2400 75 4594
Fragments arranged by size:
4594 2400 75
Acc65I G'GTAC_C
Cuts at: 2486 4390 2486
Size: 1904 5165
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2237 3192 3256 5132 6516 6784 1632
Size: 590 15 955 64 1876 1384 268 1917
Fragments arranged by size:
1917 1876 1384 955 590 268 64 15
AflII C'TTAA_G
Cuts at: 820 1017 4016 820
Size: 197 2999 3873
Fragments arranged by size:
3873 2999 197
AflIII A'CryG_T
Cuts at: 1203 2574 2656 3767 5968 6945 1203
Size: 1371 82 1111 2201 977 1327
Fragments arranged by size:
2201 1371 1327 1111 977 82
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 3612 1166
Size: 2446 4623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3815 1184
Size: 2631 4438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3121 3153 3121
Size: 32 7037
AlwNI CAG_nnn'CTG
Cuts at: 2131 2482 3014 4812 6384 2131
Size: 351 532 1798 1572 2816
Fragments arranged by size:
2816 1798 1572 532 351
ApaI G_GGCC'C
Cuts at: 2088 4154 2088
Size: 2066 5003
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 2377 4259 4268 4378 4604 4756 6865 1493
Size: 884 1882 9 110 226 152 2109 1697
Fragments arranged by size:
2109 1882 1697 884 226 152 110 9
AseI AT'TA_AT
Cuts at: 160 1032 5623 160
Size: 872 4591 1606
Fragments arranged by size:
4591 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 3546 4394 1870
Size: 73 322 1281 848 4545
Fragments arranged by size:
4545 1281 848 322 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2080 2113 2470 2503 2080
Size: 33 357 33 6646
Fragments arranged by size:
6646 357 33 33
BamHI G'GATC_C
Cuts at: 3485 4399 4766 3485
Size: 914 367 5788
Fragments arranged by size:
5788 914 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2486 4390 5845 618
Size: 325 217 627 699 1904 1455 1842
Fragments arranged by size:
1904 1842 1455 699 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2117 2144 2186 2303 3054 3860 3955
Size: 697 29 27 42 117 751 806 95
Cuts at: 3955 4154 4388 1391
Size: 199 234 4072
Fragments arranged by size:
4072 806 751 697 234 199 117 95
42 29 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2630 928
Size: 971 731 5367
Fragments arranged by size:
5367 971 731
BbvCI CC'TCA_GC
Cuts at: 2288 2288
Size: 7069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5260 5294 1903
Size: 34 50 34 3239 34 3678
Fragments arranged by size:
3678 3239 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 4961 6171 3857
Size: 1104 1210 4755
Fragments arranged by size:
4755 1210 1104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7069
BfrBI ATG'CAT
Cuts at: 2807 2807
Size: 7069
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3395 3478 3576
Size: 107 122 71 631 605 1723 83 98
Cuts at: 3576 5680 136
Size: 2104 1525
Fragments arranged by size:
2104 1723 1525 631 605 122 107 98
83 71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 4486 4486
Size: 7069
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2233 3887 4154 4367 5130 6286 1790
Size: 298 145 1654 267 213 763 1156 2573
Fragments arranged by size:
2573 1654 1156 763 298 267 213 145
BmgBI CAC'GTC
Cuts at: 1318 4335 1318
Size: 3017 4052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2246 3484 4800
Size: 416 53 83 399 24 829 1238 1316
Cuts at: 4800 5758 442
Size: 958 1753
Fragments arranged by size:
1753 1316 1238 958 829 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3706 3706
Size: 7069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2815 3089 5729 1239
Size: 1086 490 274 2640 2579
Fragments arranged by size:
2640 2579 1086 490 274
Bpu10I CC'TnA_GC
Cuts at: 2288 3691 2288
Size: 1403 5666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2310 3215 3233 5194 6059 6357 2310
Size: 905 18 1961 865 298 3022
Fragments arranged by size:
3022 1961 905 865 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5732 882
Size: 617 4233 2219
Fragments arranged by size:
4233 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2659 4356 4860 493
Size: 711 1455 1697 504 2702
Fragments arranged by size:
2702 1697 1455 711 504
BsaBI GATnn'nnATC
Cuts at: 7063 7063
Size: 7069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4876 5258 5893 275
Size: 53 83 186 936 3343 382 635 1451
Fragments arranged by size:
3343 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5502 5900 6174 6321 6732 5502
Size: 398 274 147 411 5839
Fragments arranged by size:
5839 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2340 2370 2915 2945 3249
Size: 30 165 30 937 30 545 30 304
Cuts at: 3249 3279 3644 3674 1178
Size: 30 365 30 4573
Fragments arranged by size:
4573 937 545 365 304 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3521 4223 1186
Size: 2335 702 4032
Fragments arranged by size:
4032 2335 702
BseYI C'CCAG_C
Cuts at: 2053 3481 3525 6272 6768 2053
Size: 1428 44 2747 496 2354
Fragments arranged by size:
2747 2354 1428 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3727 3826 3889 1163
Size: 573 1991 99 63 4343
Fragments arranged by size:
4343 1991 573 99 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 2030 3814 4252 4519 5280
Size: 275 186 17 12 1784 438 267 761
Cuts at: 5280 6308 1540
Size: 1028 2301
Fragments arranged by size:
2301 1784 1028 761 438 275 267 186
17 12
BsiHKAI G_wGCw'C
Cuts at: 2117 2162 3054 3503 3692 5130 5215 6286 2117
Size: 45 892 449 189 1438 85 1071 2900
Fragments arranged by size:
2900 1438 1071 892 449 189 85 45
BsmI GAATG_Cn'
Cuts at: 3542 4580 4673 3542
Size: 1038 93 5938
Fragments arranged by size:
5938 1038 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2117 2144 2162 2186 2233 2303
Size: 399 298 29 27 18 24 47 70
Cuts at: 2303 3054 3503 3692 3860 3887 3955 4154 4367
Size: 751 449 189 168 27 68 199 213
Cuts at: 4367 4388 5130 5215 6286 1391
Size: 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 449 399 298 213
199 189 168 85 70 68 47 29
27 27 24 21 18
BspEI T'CCGG_A
Cuts at: 6732 6732
Size: 7069
BspHI T'CATG_A
Cuts at: 4958 6688 4958
Size: 1730 5339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 2535 2639 3209 3788 3899 3980 4424
Size: 1545 146 104 570 579 111 81 444
Cuts at: 4424 844
Size: 3489
Fragments arranged by size:
3489 1545 579 570 444 146 111 104
81
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 4956 1394
Size: 429 209 2924 3507
Fragments arranged by size:
3507 2924 429 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 3103 3442 3799 5564 5738 65
Size: 1183 1855 339 357 1765 174 1396
Fragments arranged by size:
1855 1765 1396 1183 357 339 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3743 5713 5900 1700
Size: 110 1933 1970 187 2869
Fragments arranged by size:
2869 1970 1933 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 5129 6141 5129
Size: 1012 6057
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3642 794
Size: 2848 4221
BstBI TT'CG_AA
Cuts at: 2372 5873 2372
Size: 3501 3568
BstEII G'GTnAC_C
Cuts at: 1184 2680 3367 1184
Size: 1496 687 4886
Fragments arranged by size:
4886 1496 687
BstXI CCAn_nnnn'nTGG
Cuts at: 2941 2941
Size: 7069
BstYI r'GATC_y
Cuts at: 1972 3485 4399 4766 5151 5168 6620 6706 6718
Size: 1513 914 367 385 17 1452 86 12
Cuts at: 6718 7064 1972
Size: 346 1977
Fragments arranged by size:
1977 1513 1452 914 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2018 2940 3084 3336 3697 3814 513
Size: 552 953 922 144 252 361 117 3768
Fragments arranged by size:
3768 953 922 552 361 252 144 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3404 487
Size: 683 56 2178 4152
Fragments arranged by size:
4152 2178 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 2903 3015 3619 4044 4585 5379 5399 2132
Size: 771 112 604 425 541 794 20 3802
Fragments arranged by size:
3802 794 771 604 541 425 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4373 4373
Size: 7069
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4073 6076 809
Size: 507 2757 2003 1802
Fragments arranged by size:
2757 2003 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2027 2360
Size: 54 1425 50 275 18 185 12 333
Cuts at: 2360 2496 3700 3811 4516 5405 7034 8
Size: 136 1204 111 705 889 1629 43
Fragments arranged by size:
1629 1425 1204 889 705 333 275 185
136 111 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2015 2027 3811 4516 1537
Size: 275 203 12 1784 705 4090
Fragments arranged by size:
4090 1784 705 275 203 12
EarI CTCTTCn'nnn_
Cuts at: 1353 3168 4125 4999 1353
Size: 1815 957 874 3423
Fragments arranged by size:
3423 1815 957 874
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5656 6030 6176 387
Size: 1064 138 4067 374 146 1280
Fragments arranged by size:
4067 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2115 3052 2115
Size: 937 6132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2237 2325 2815 3089 3192 3256
Size: 393 590 15 88 490 274 103 64
Cuts at: 3256 5132 5729 6516 6784 1239
Size: 1876 597 787 268 1524
Fragments arranged by size:
1876 1524 787 597 590 490 393 274
268 103 88 64 15
EcoO109I rG'GnC_Cy
Cuts at: 2084 2170 2283 2596 3441 4150 4151 2084
Size: 86 113 313 845 709 1 5002
Fragments arranged by size:
5002 845 709 313 113 86 1
EcoRI G'AATT_C
Cuts at: 4259 4378 4756 4259
Size: 119 378 6572
Fragments arranged by size:
6572 378 119
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 2054 2063 6216 1678
Size: 376 9 4153 2531
Fragments arranged by size:
4153 2531 376 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2936 2963 2968
Size: 32 135 32 713 32 619 27 5
Cuts at: 2968 2995 3644 3676 3986 4018 4319 4351 5710
Size: 27 649 32 310 32 301 32 1359
Cuts at: 5710 5742 5784 5816 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1359 713 649 619 310 301 135
42 32 32 32 32 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4413 4659 669
Size: 988 281 2475 246 3079
Fragments arranged by size:
3079 2475 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7069
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KpnI G_GTAC'C
Cuts at: 2490 4394 2490
Size: 1904 5165
MfeI C'AATT_G
Cuts at: 4668 4668
Size: 7069
MluI A'CGCG_T
Cuts at: 3767 6945 3767
Size: 3178 3891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3759 6183 6367 1305
Size: 2454 2424 184 2007
Fragments arranged by size:
2454 2424 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 3702 10
Size: 54 1425 343 666 1204 3377
Fragments arranged by size:
3377 1425 1204 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2504 2939 3612 3782 4163 5027
Size: 648 1224 114 435 673 170 381 864
Cuts at: 5027 5386 5545 518
Size: 359 159 2042
Fragments arranged by size:
2042 1224 864 673 648 435 381 359
170 159 114
MspA1I CmG'CkG
Cuts at: 1606 2020 3574 3711 3810 3816 3837 4485 5162
Size: 414 1554 137 99 6 21 648 677
Cuts at: 5162 6310 6555 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1554 1148 677 648 414 245 137
99 21 6
NcoI C'CATG_G
Cuts at: 513 1065 2940 3084 3697 513
Size: 552 1875 144 613 3885
Fragments arranged by size:
3885 1875 613 552 144
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 2015 3811 4516 2015
Size: 1796 705 4568
Fragments arranged by size:
4568 1796 705
NsiI A_TGCA'T
Cuts at: 2809 2809
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 2578 3787 4427 5972 1132
Size: 97 1349 1209 640 1545 2229
Fragments arranged by size:
2229 1545 1349 1209 640 97
PasI CC'CwG_GG
Cuts at: 1348 3059 1348
Size: 1711 5358
PciI A'CATG_T
Cuts at: 2574 5968 2574
Size: 3394 3675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2482 2744 2818 1108
Size: 1374 262 74 5359
Fragments arranged by size:
5359 1374 262 74
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PmlI CAC'GTG
Cuts at: 2659 2659
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3441 3441
Size: 7069
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 2084 4150 2084
Size: 2066 5003
PstI C_TGCA'G
Cuts at: 830 3569 4421 830
Size: 2739 852 3478
Fragments arranged by size:
3478 2739 852
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 3711 3837 3711
Size: 126 6943
SacI G_AGCT'C
Cuts at: 2117 3054 2117
Size: 937 6132
SacII CC_GC'GG
Cuts at: 2021 3817 2021
Size: 1796 5273
SalI G'TCGA_C
Cuts at: 1936 4411 1936
Size: 2475 4594
SapI GCTCTTCn'nnn_
Cuts at: 4125 4125
Size: 7069
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 2271 3641 4088 5317 7029 2271
Size: 1370 447 1229 1712 2311
Fragments arranged by size:
2311 1712 1370 1229 447
SexAI A'CCwGG_T
Cuts at: 2676 2676
Size: 7069
SfcI C'TryA_G
Cuts at: 826 1045 2769 3043 3565 4417 5552 6233 6424
Size: 219 1724 274 522 852 1135 681 191
Cuts at: 6424 826
Size: 1471
Fragments arranged by size:
1724 1471 1135 852 681 522 274 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7069
SmaI CCC'GGG
Cuts at: 1872 3548 4396 1872
Size: 1676 848 4545
Fragments arranged by size:
4545 1676 848
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2325 3230 3248 4016 5173 6074
Size: 197 926 382 905 18 768 1157 901
Cuts at: 6074 6336 820
Size: 262 1553
Fragments arranged by size:
1553 1157 926 905 901 768 382 262
197 18
SnaBI TAC'GTA
Cuts at: 493 4860 493
Size: 4367 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 4427 1132
Size: 3295 3774
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7069
StyI C'CwwG_G
Cuts at: 513 1065 2940 3084 3285 3697 4146 4508 513
Size: 552 1875 144 201 412 449 362 3074
Fragments arranged by size:
3074 1875 552 449 412 362 201 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 4266 5096 5113 5266 5451 5896
Size: 1255 936 1750 830 17 153 185 445
Cuts at: 5896 325
Size: 1498
Fragments arranged by size:
1750 1498 1255 936 830 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2269 3639 4086
Size: 274 80 33 51 1443 292 1370 447
Cuts at: 4086 5315 7027 96
Size: 1229 1712 138
Fragments arranged by size:
1712 1443 1370 1229 447 292 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 2450 2482 3249 3281 3710 3742 7029
Size: 32 78 32 767 32 429 32 3287
Cuts at: 7029 7061 2340
Size: 32 2348
Fragments arranged by size:
3287 2348 767 429 78 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4070 1316
Size: 2754 4315
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3334 3710 4120 3334
Size: 376 410 6283
Fragments arranged by size:
6283 410 376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7069
XmaI C'CCGG_G
Cuts at: 1870 3546 4394 1870
Size: 1676 848 4545
Fragments arranged by size:
4545 1676 848
XmnI GAAnn'nnTTC
Cuts at: 2378 5198 2378
Size: 2820 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4877 276
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI