Restriction map for
pFN21AB5613
(Circular) (Six-base) MAPSORT of: pFN21AB5613.seq Check: 657 from: 1 to: 6208
pFN21AB5613 6208 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2793 4271 5655 5923 1632
Size: 512 649 1478 1384 268 1917
Fragments arranged by size:
1917 1478 1384 649 512 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 2420 3218 3951 5523 2420
Size: 798 733 1572 3105
Fragments arranged by size:
3105 1572 798 733
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2944 3172 3517 3743 3895 6004 1493
Size: 1451 228 345 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 345 228 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2880 4762 160
Size: 872 1848 1882 1606
Fragments arranged by size:
1882 1848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 3042 3533 1870
Size: 73 1099 491 4545
Fragments arranged by size:
4545 1099 491 73
BamHI G'GATC_C
Cuts at: 2571 3538 3905 2571
Size: 967 367 4874
Fragments arranged by size:
4874 967 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3271 3529 4984 618
Size: 325 217 627 381 1103 258 1455 1842
Fragments arranged by size:
1842 1455 1103 627 381 325 258 217
BanII G_rGCy'C
Cuts at: 1391 3527 1391
Size: 2136 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5237
BbvCI CC'TCA_GC
Cuts at: 2886 2886
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3225 3259 4399 4433 1903
Size: 34 1288 34 1140 34 3678
Fragments arranged by size:
3678 1288 1140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BfrBI ATG'CAT
Cuts at: 2738 3256 2738
Size: 518 5690
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2104 4819 136
Size: 107 122 71 631 605 432 2715 1525
Fragments arranged by size:
2715 1525 631 605 432 122 107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 4269 5425 1790
Size: 2479 1156 2573
Fragments arranged by size:
2573 2479 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2948 3939 4897
Size: 416 53 83 399 24 1531 991 958
Cuts at: 4897 442
Size: 1753
Fragments arranged by size:
1753 1531 991 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2346 2581 2649 3004 3171 3402 4868
Size: 959 148 235 68 355 167 231 1466
Cuts at: 4868 1239
Size: 2579
Fragments arranged by size:
2579 1466 959 355 235 231 167 148
68
Bpu10I CC'TnA_GC
Cuts at: 2886 2886
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4333 5198 5496 4333
Size: 865 298 5045
Fragments arranged by size:
5045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3147 4871 882
Size: 617 1648 1724 2219
Fragments arranged by size:
2219 1724 1648 617
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 3255 6202 3255
Size: 2947 3261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2673 4015 4397 5032
Size: 53 83 186 936 1140 1342 382 635
Cuts at: 5032 275
Size: 1451
Fragments arranged by size:
1451 1342 1140 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5983
Fragments arranged by size:
5983 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 2903 3087 1186
Size: 1201 516 184 4307
Fragments arranged by size:
4307 1201 516 184
BseYI C'CCAG_C
Cuts at: 3218 3286 5411 5907 3218
Size: 68 2125 496 3519
Fragments arranged by size:
3519 2125 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 1163
Size: 573 402 5233
Fragments arranged by size:
5233 573 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 4269 4354 5425 2214
Size: 2055 85 1071 2997
Fragments arranged by size:
2997 2055 1071 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 3045 3045
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 3527 4269 4354 5425 1391
Size: 399 424 1313 742 85 1071 2174
Fragments arranged by size:
2174 1313 1071 742 424 399 85
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 3563 844
Size: 1214 1505 3489
Fragments arranged by size:
3489 1505 1214
BsrBI CCG'CTC
Cuts at: 1394 1823 2932 4095 1394
Size: 429 1109 1163 3507
Fragments arranged by size:
3507 1163 1109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4703 4877 65
Size: 1183 3455 174 1396
Fragments arranged by size:
3455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2420 794
Size: 1626 4582
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 2045 3050 1184
Size: 861 1005 4342
Fragments arranged by size:
4342 1005 861
BstYI r'GATC_y
Cuts at: 1972 2571 3538 3905 4290 4307 5759 5845 5857
Size: 599 967 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 967 599 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2004 2823 513
Size: 552 939 819 3898
Fragments arranged by size:
3898 939 819 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2916 2922 3724 4518 4538 2916
Size: 6 802 794 20 4586
Fragments arranged by size:
4586 802 794 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2892 2927 529
Size: 35 2328 35 3810
Fragments arranged by size:
3810 2328 35 35
DraI TTT'AAA
Cuts at: 2668 3512 2668
Size: 844 5364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2826 3655 4544
Size: 54 1425 50 275 18 996 829 889
Cuts at: 4544 6173 8
Size: 1629 43
Fragments arranged by size:
1629 1425 996 889 829 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2680 3149 3317 4138 1353
Size: 767 560 469 168 821 3423
Fragments arranged by size:
3423 821 767 560 469 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2198 2346 2581 2649 2793 3004
Size: 393 512 54 148 235 68 144 211
Cuts at: 3004 3171 3402 4271 4868 5655 5923 1239
Size: 167 231 869 597 787 268 1524
Fragments arranged by size:
1524 869 787 597 512 393 268 235
231 211 167 148 144 68 54
EcoO109I rG'GnC_Cy
Cuts at: 2266 2266
Size: 6208
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
EcoRV GAT'ATC
Cuts at: 2430 2430
Size: 6208
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2568 5355 1678
Size: 890 2787 2531
Fragments arranged by size:
2787 2531 890
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2172 2204 4849 4881 4923
Size: 32 135 32 600 32 2645 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2645 2626 600 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 3552 3798 669
Size: 988 281 127 1487 246 3079
Fragments arranged by size:
3079 1487 988 281 246 127
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 2065 3798 2065
Size: 1733 4475
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 10
Size: 54 1425 343 996 3390
Fragments arranged by size:
3390 1425 996 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4166 4525 4684 518
Size: 648 3000 359 159 2042
Fragments arranged by size:
3000 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2513 2559 3107 3624 4301 5449 5694
Size: 516 391 46 548 517 677 1148 245
Cuts at: 5694 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 548 517 516 391 245
46
NcoI C'CATG_G
Cuts at: 513 1065 2004 2823 513
Size: 552 939 819 3898
Fragments arranged by size:
3898 939 819 552
NdeI CA'TA_TG
Cuts at: 387 2740 387
Size: 2353 3855
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NsiI A_TGCA'T
Cuts at: 2740 3258 2740
Size: 518 5690
NspI r_CATG'y
Cuts at: 1132 1229 2239 3309 3566 5111 1132
Size: 97 1010 1070 257 1545 2229
Fragments arranged by size:
2229 1545 1070 1010 257 97
PasI CC'CwG_GG
Cuts at: 1348 2083 1348
Size: 735 5473
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2266
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 3383 1613
Size: 1770 4438
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2055 2416 3560 830
Size: 1225 361 1144 3478
Fragments arranged by size:
3478 1225 1144 361
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2122 2513 2559 3107 2122
Size: 391 46 548 5223
Fragments arranged by size:
5223 548 391 46
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SapI GCTCTTCn'nnn_
Cuts at: 3149 3149
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 2055 3560 2055
Size: 1505 4703
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 2051 2412 2526 3556 4691 5372 5563
Size: 219 1006 361 114 1030 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1471 1135 1030 1006 681 361 219 191
114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3042 4312 5213 5475 820
Size: 197 926 1099 1270 901 262 1553
Fragments arranged by size:
1553 1270 1099 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2823 3647 513
Size: 552 939 819 824 3074
Fragments arranged by size:
3074 939 824 819 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2377 4235 4252 4405 4590 5035 325
Size: 1255 797 1858 17 153 185 445 1498
Fragments arranged by size:
1858 1498 1255 797 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3350 4454 6166
Size: 274 80 33 51 1443 1373 1104 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
1712 1443 1373 1104 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2245 2277 3267 3299 6168 6200 2245
Size: 32 990 32 2869 32 2253
Fragments arranged by size:
2869 2253 990 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 3042 1943
Size: 1099 5109
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 2689 4337 2689
Size: 1648 4560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI