Restriction map for
pFN21AB5611
(Circular) (Six-base) MAPSORT of: pFN21AB5611.seq Check: 6527 from: 1 to: 5851
pFN21AB5611 5851 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2528 2528
Size: 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3194 1937
Size: 883 374 4594
Fragments arranged by size:
4594 883 374
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2682 2706 3914 5298 5566 1632
Size: 1050 24 1208 1384 268 1917
Fragments arranged by size:
1917 1384 1208 1050 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 2780 4580 2780
Size: 1800 4051
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2660 3594 5166 2660
Size: 934 1572 3345
Fragments arranged by size:
3345 1572 934
ApaI G_GGCC'C
Cuts at: 2593 2593
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 2526 3160 3386 3538 5647 1493
Size: 1033 634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1033 634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 1870
Size: 73 1233 4545
Fragments arranged by size:
4545 1233 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 4627 618
Size: 325 217 627 1385 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2593 2754 3170 1391
Size: 1202 161 416 4072
Fragments arranged by size:
4072 1202 416 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 4704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4462 136
Size: 107 122 71 631 605 2790 1525
Fragments arranged by size:
2790 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2681 5846 2681
Size: 3165 2686
BlpI GC'TnA_GC
Cuts at: 2081 3268 2081
Size: 1187 4664
Bme1580I G_kGCm'C
Cuts at: 1790 2593 3912 5068 1790
Size: 803 1319 1156 2573
Fragments arranged by size:
2573 1319 1156 803
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 2534 4511 1239
Size: 1103 192 1977 2579
Fragments arranged by size:
2579 1977 1103 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2428 2816 3976 4841 5139 2428
Size: 388 1160 865 298 3140
Fragments arranged by size:
3140 1160 865 388 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 2867 5845 2867
Size: 2978 2873
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2982 3658 4040 4675
Size: 53 83 186 936 1449 676 382 635
Cuts at: 4675 275
Size: 1451
Fragments arranged by size:
1451 1449 936 676 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2909 2939 1178
Size: 30 165 30 1506 30 4090
Fragments arranged by size:
4090 1506 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5851
BseYI C'CCAG_C
Cuts at: 3032 5054 5550 3032
Size: 2022 496 3333
Fragments arranged by size:
3333 2022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2388 1163
Size: 573 652 4626
Fragments arranged by size:
4626 652 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2278 2754 3912 3997 5068 2278
Size: 476 1158 85 1071 3061
Fragments arranged by size:
3061 1158 1071 476 85
BsmI GAATG_Cn'
Cuts at: 2539 2948 3362 3455 2539
Size: 409 414 93 4935
Fragments arranged by size:
4935 414 409 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2278 2593 2754 3170 3912 3997 5068
Size: 399 488 315 161 416 742 85 1071
Cuts at: 5068 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 488 416 399 315 161
85
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2528 3206 844
Size: 1684 678 3489
Fragments arranged by size:
3489 1684 678
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2815 4346 4520 65
Size: 1183 1567 1531 174 1396
Fragments arranged by size:
1567 1531 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 2681 2768 3181 3548 3933 3950 5402 5488
Size: 709 87 413 367 385 17 1452 86
Cuts at: 5488 5500 5846 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 709 413 385 367 346 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2620 513
Size: 552 939 616 3744
Fragments arranged by size:
3744 939 616 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 2913 3367 4161 4181 2913
Size: 454 794 20 4583
Fragments arranged by size:
4583 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 2260 3155 2260
Size: 895 4956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816
Size: 54 1425 50 275 18 1468 889 1629
Cuts at: 5816 8
Size: 43
Fragments arranged by size:
1629 1468 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2742 3781 1353
Size: 1389 1039 3423
Fragments arranged by size:
3423 1389 1039
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2752 2752
Size: 5851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2534 2682 2706 3914 4511 5298
Size: 393 710 192 148 24 1208 597 787
Cuts at: 5298 5566 1239
Size: 268 1524
Fragments arranged by size:
1524 1208 787 710 597 393 268 192
148 24
EcoO109I rG'GnC_Cy
Cuts at: 2589 2590 2630 2589
Size: 1 40 5810
Fragments arranged by size:
5810 40 1
EcoRI G'AATT_C
Cuts at: 2526 3160 3538 2526
Size: 634 378 4839
Fragments arranged by size:
4839 634 378
FspI TGC'GCA
Cuts at: 3008 4357 3008
Size: 1349 4502
FspAI rTGC'GCAy
Cuts at: 3008 3008
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 4998 1678
Size: 3320 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2766 2798 3110 3116 3142
Size: 32 135 32 1194 32 312 6 26
Cuts at: 3142 3148 4492 4524 4566 4598 1373
Size: 6 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 1194 312 135 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 2821 3195 3441 669
Size: 988 281 447 436 374 246 3079
Fragments arranged by size:
3079 988 447 436 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 4965 5149 1305
Size: 1602 2058 184 2007
Fragments arranged by size:
2058 2007 1602 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 3809 4168 4327 518
Size: 648 1692 951 359 159 2042
Fragments arranged by size:
2042 1692 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2390 2512 3267 3944 5092 5337 1606
Size: 784 122 755 677 1148 245 2120
Fragments arranged by size:
2120 1148 784 755 677 245 122
NcoI C'CATG_G
Cuts at: 513 1065 2004 2620 513
Size: 552 939 616 3744
Fragments arranged by size:
3744 939 616 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2092 2192 2718 3209 4754 1132
Size: 97 863 100 526 491 1545 2229
Fragments arranged by size:
2229 1545 863 526 491 100 97
PasI CC'CwG_GG
Cuts at: 1348 2050 1348
Size: 702 5149
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2630 2630
Size: 5851
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2589 2589
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2272 3203 830
Size: 1442 931 3478
Fragments arranged by size:
3478 1442 931
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2390 2390
Size: 5851
SacI G_AGCT'C
Cuts at: 2754 2754
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 2819 3193 1936
Size: 883 374 4594
Fragments arranged by size:
4594 883 374
SapI GCTCTTCn'nnn_
Cuts at: 2742 2742
Size: 5851
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2268 2391 3199 4334 5015 5206 826
Size: 219 1223 123 808 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 808 681 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2443 2795 3955 4856 5118 820
Size: 197 926 500 352 1160 901 262 1553
Fragments arranged by size:
1553 1160 926 901 500 352 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 2092 2192 3209 1132
Size: 960 100 1017 3774
Fragments arranged by size:
3774 1017 960 100
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2593 2620 3290 513
Size: 552 939 589 27 670 3074
Fragments arranged by size:
3074 939 670 589 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2902 3878 3895 4048 4233 4678 325
Size: 1255 1322 976 17 153 185 445 1498
Fragments arranged by size:
1498 1322 1255 976 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 2993 1316
Size: 1677 4174
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 2526 3980 2526
Size: 1454 4397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI