Restriction map for
pFN21AB5610
(Circular) (Six-base) MAPSORT of: pFN21AB5610.seq Check: 7200 from: 1 to: 7741
pFN21AB5610 7741 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5551 278
Size: 53 83 186 936 4015 2468
Fragments arranged by size:
4015 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3748 5084 1937
Size: 1811 1336 4594
Fragments arranged by size:
4594 1811 1336
Acc65I G'GTAC_C
Cuts at: 5062 5062
Size: 7741
AclI AA'CG_TT
Cuts at: 1563 5868 6241 1563
Size: 4305 373 3063
Fragments arranged by size:
4305 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2937 4311 4361 4440 4766 5804 7188 7456
Size: 1305 1374 50 79 326 1038 1384 268
Cuts at: 7456 1632
Size: 1917
Fragments arranged by size:
1917 1384 1374 1305 1038 326 268 79
50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7544
AflIII A'CryG_T
Cuts at: 1203 2766 6640 7617 1203
Size: 1563 3874 977 1327
Fragments arranged by size:
3874 1563 1327 977
AgeI A'CCGG_T
Cuts at: 6572 6572
Size: 7741
AhdI GACnn_n'nnGTC
Cuts at: 6470 6470
Size: 7741
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2820 3189 4382 1184
Size: 1636 369 1193 4543
Fragments arranged by size:
4543 1636 1193 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 7709
AlwNI CAG_nnn'CTG
Cuts at: 3692 5039 5484 7056 3692
Size: 1347 445 1572 4377
Fragments arranged by size:
4377 1572 1347 445
ApaLI G'TGCA_C
Cuts at: 4088 5798 6954 4088
Size: 1710 1156 4875
Fragments arranged by size:
4875 1710 1156
ApoI r'AATT_y
Cuts at: 1493 2306 2354 2406 3626 4121 5050 5276 5428
Size: 813 48 52 1220 495 929 226 152
Cuts at: 5428 7537 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1220 929 813 495 226 152
52 48
AseI AT'TA_AT
Cuts at: 160 1032 2841 6295 160
Size: 872 1809 3454 1606
Fragments arranged by size:
3454 1809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7741
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2135 4228 5066 1870
Size: 73 122 70 2093 838 4545
Fragments arranged by size:
4545 2093 838 122 73 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4101 4134 4101
Size: 33 7708
BamHI G'GATC_C
Cuts at: 5071 5438 5071
Size: 367 7374
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2292 3896 5062 6517 618
Size: 325 217 627 505 1604 1166 1455 1842
Fragments arranged by size:
1842 1604 1455 1166 627 505 325 217
BanII G_rGCy'C
Cuts at: 1391 2215 3915 5060 1391
Size: 824 1700 1145 4072
Fragments arranged by size:
4072 1700 1145 824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2271 3786 4067 928
Size: 971 279 93 1515 281 4602
Fragments arranged by size:
4602 1515 971 281 279 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5932 5966 1903
Size: 34 3995 34 3678
Fragments arranged by size:
3995 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4102 5633 6843 4102
Size: 1531 1210 5000
Fragments arranged by size:
5000 1531 1210
BclI T'GATC_A
Cuts at: 1548 3823 4237 4884 1548
Size: 2275 414 647 4405
Fragments arranged by size:
4405 2275 647 414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6352 136
Size: 107 122 71 631 605 4680 1525
Fragments arranged by size:
4680 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3031 3834 4488 7736 3031
Size: 803 654 3248 3036
Fragments arranged by size:
3248 3036 803 654
BlpI GC'TnA_GC
Cuts at: 3909 5158 3909
Size: 1249 6492
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2422 4092 5802 6958 1790
Size: 388 244 1670 1710 1156 2573
Fragments arranged by size:
2573 1710 1670 1156 388 244
BmgBI CAC'GTC
Cuts at: 1318 2107 2528 1318
Size: 789 421 6531
Fragments arranged by size:
6531 789 421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4163 4273 5472
Size: 416 53 83 399 24 2746 110 1199
Cuts at: 5472 6430 442
Size: 958 1753
Fragments arranged by size:
2746 1753 1199 958 416 399 110 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7741
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2897 3656 2897
Size: 759 6982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6401 1239
Size: 5162 2579
Bpu10I CC'TnA_GC
Cuts at: 3973 3973
Size: 7741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3476 4962 5866 6731 7029 3476
Size: 1486 904 865 298 4188
Fragments arranged by size:
4188 1486 904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2935 6404 882
Size: 617 1436 3469 2219
Fragments arranged by size:
3469 2219 1436 617
BsaAI yAC'GTr
Cuts at: 493 1204 4681 5532 493
Size: 711 3477 851 2702
Fragments arranged by size:
3477 2702 851 711
BsaBI GATnn'nnATC
Cuts at: 3822 7735 3822
Size: 3913 3828
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5548 5930 6565 275
Size: 53 83 186 936 4015 382 635 1451
Fragments arranged by size:
4015 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6174 6572 6846 6993 7404 6174
Size: 398 274 147 411 6511
Fragments arranged by size:
6511 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2244 2274 2370 2400 2756
Size: 30 165 30 841 30 96 30 356
Cuts at: 2756 2786 1178
Size: 30 6133
Fragments arranged by size:
6133 841 356 165 96 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4236 1186
Size: 3050 4691
BseYI C'CCAG_C
Cuts at: 2177 2232 6944 7440 2177
Size: 55 4712 496 2478
Fragments arranged by size:
4712 2478 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2607 4435 1163
Size: 573 871 1828 4469
Fragments arranged by size:
4469 1828 871 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5191 5952 6980 1540
Size: 275 186 3190 761 1028 2301
Fragments arranged by size:
3190 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2215 2878 3520 3915 4092 4272 5802 5887 6958
Size: 663 642 395 177 180 1530 85 1071
Cuts at: 6958 2215
Size: 2998
Fragments arranged by size:
2998 1530 1071 663 642 395 180 177
85
BsmI GAATG_Cn'
Cuts at: 4848 5252 5345 4848
Size: 404 93 7244
Fragments arranged by size:
7244 404 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2215 2422 2878 3520 3915 4092
Size: 399 388 37 207 456 642 395 177
Cuts at: 4092 4272 5060 5802 5887 6958 1391
Size: 180 788 742 85 1071 2174
Fragments arranged by size:
2174 1071 788 742 642 456 399 395
388 207 180 177 85 37
BspEI T'CCGG_A
Cuts at: 7404 7404
Size: 7741
BspHI T'CATG_A
Cuts at: 5011 5630 7360 5011
Size: 619 1730 5392
Fragments arranged by size:
5392 1730 619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2248 2917 3374 3525 5096 844
Size: 1404 669 457 151 1571 3489
Fragments arranged by size:
3489 1571 1404 669 457 151
BsrBI CCG'CTC
Cuts at: 1394 1823 4543 5628 1394
Size: 429 2720 1085 3507
Fragments arranged by size:
3507 2720 1085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4371 6236 6410 65
Size: 1183 3123 1865 174 1396
Fragments arranged by size:
3123 1865 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 6385 6572 1700
Size: 110 479 4096 187 2869
Fragments arranged by size:
4096 2869 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 4941 96
Size: 4845 2896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7741
BssSI C'ACGA_G
Cuts at: 2204 5801 6813 2204
Size: 3597 1012 3132
Fragments arranged by size:
3597 3132 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3068 794
Size: 2274 5467
BstBI TT'CG_AA
Cuts at: 6545 6545
Size: 7741
BstEII G'GTnAC_C
Cuts at: 1184 4825 1184
Size: 3641 4100
BstYI r'GATC_y
Cuts at: 1972 3031 3834 4488 4567 5071 5438 5823 5840
Size: 1059 803 654 79 504 367 385 17
Cuts at: 5840 7292 7378 7390 7736 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1059 803 654 504 385 367
346 86 79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2044 2160 513
Size: 552 939 14 26 116 6094
Fragments arranged by size:
6094 939 552 116 26 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7002
Fragments arranged by size:
7002 683 56
BtsI GCAGTG_nn'
Cuts at: 3016 3173 3371 3593 3929 5257 6051 6071 3016
Size: 157 198 222 336 1328 794 20 4686
Fragments arranged by size:
4686 1328 794 336 222 198 157 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7706
DraI TTT'AAA
Cuts at: 3348 4960 5045 3348
Size: 1612 85 6044
Fragments arranged by size:
6044 1612 85
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6748 809
Size: 507 5432 1802
Fragments arranged by size:
5432 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4970 5188 6077
Size: 54 1425 50 275 18 3140 218 889
Cuts at: 6077 7706 8
Size: 1629 43
Fragments arranged by size:
3140 1629 1425 889 275 218 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5188 1537
Size: 275 3376 4090
Fragments arranged by size:
4090 3376 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2888 5671 1353
Size: 1535 2783 3423
Fragments arranged by size:
3423 2783 1535
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2004 6328 6702 6848 387
Size: 1064 138 415 4324 374 146 1280
Fragments arranged by size:
4324 1280 1064 415 374 146 138
EcoICRI GAG'CTC
Cuts at: 2213 3913 2213
Size: 1700 6041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2937 4311 4361 4440 4766 5804 6401
Size: 393 1305 1374 50 79 326 1038 597
Cuts at: 6401 7188 7456 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1374 1305 1038 787 597 393 326
268 79 50
EcoO109I rG'GnC_Cy
Cuts at: 2260 3537 2260
Size: 1277 6464
EcoRI G'AATT_C
Cuts at: 5050 5428 5050
Size: 378 7363
EcoRV GAT'ATC
Cuts at: 3242 4807 3242
Size: 1565 6176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3461 4208 3461
Size: 747 6994
FspI TGC'GCA
Cuts at: 6247 6247
Size: 7741
HaeII r_GCGC'y
Cuts at: 1678 4862 6888 1678
Size: 3184 2026 2531
Fragments arranged by size:
3184 2531 2026
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 2882 2914 2976
Size: 32 135 32 672 32 606 32 62
Cuts at: 2976 3008 3291 3323 3592 3624 4222 4254 6382
Size: 32 283 32 269 32 598 32 2128
Cuts at: 6382 6414 6456 6488 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2128 672 606 598 283 269 135
62 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3749 5085 5331 669
Size: 988 281 1811 1336 246 3079
Fragments arranged by size:
3079 1811 1336 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3193 748
Size: 2445 5296
HpaI GTT'AAC
Cuts at: 5331 5331
Size: 7741
KpnI G_GTAC'C
Cuts at: 5066 5066
Size: 7741
MfeI C'AATT_G
Cuts at: 2301 3063 4797 5340 2301
Size: 762 1734 543 4702
Fragments arranged by size:
4702 1734 762 543
MluI A'CGCG_T
Cuts at: 7617 7617
Size: 7741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2449 2650 3654 6855 7039 1305
Size: 1144 201 1004 3201 184 2007
Fragments arranged by size:
3201 2007 1144 1004 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5919
Fragments arranged by size:
5919 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4323 4693 4868 5016 5699 6058 6217
Size: 648 3157 370 175 148 683 359 159
Cuts at: 6217 518
Size: 2042
Fragments arranged by size:
3157 2042 683 648 370 359 175 159
148
MspA1I CmG'CkG
Cuts at: 1606 2020 2046 3689 5157 5834 6982 7227 1606
Size: 414 26 1643 1468 677 1148 245 2120
Fragments arranged by size:
2120 1643 1468 1148 677 414 245 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6250
Fragments arranged by size:
6250 939 552
NdeI CA'TA_TG
Cuts at: 387 3076 387
Size: 2689 5052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7741
NotI GC'GGCC_GC
Cuts at: 5188 5188
Size: 7741
NspI r_CATG'y
Cuts at: 1132 1229 2478 2770 3187 5099 6644 1132
Size: 97 1249 292 417 1912 1545 2229
Fragments arranged by size:
2229 1912 1545 1249 417 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7741
PciI A'CATG_T
Cuts at: 2766 6640 2766
Size: 3874 3867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 3197 1108
Size: 1812 277 5652
Fragments arranged by size:
5652 1812 277
PfoI T'CCnGG_A
Cuts at: 2189 2189
Size: 7741
PmeI GTTT'AAAC
Cuts at: 5045 5045
Size: 7741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 5810 5842 7323 7355 2756
Size: 32 3022 32 1481 32 3142
Fragments arranged by size:
3142 3022 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 3537 2260
Size: 1277 6464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7741
PsiI TTA'TAA
Cuts at: 4785 5311 4785
Size: 526 7215
PstI C_TGCA'G
Cuts at: 830 3936 4210 5093 830
Size: 3106 274 883 3478
Fragments arranged by size:
3478 3106 883 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7741
PvuII CAG'CTG
Cuts at: 3689 3689
Size: 7741
SacI G_AGCT'C
Cuts at: 2215 3915 2215
Size: 1700 6041
SacII CC_GC'GG
Cuts at: 2021 2047 2021
Size: 26 7715
SalI G'TCGA_C
Cuts at: 1936 3747 5083 1936
Size: 1811 1336 4594
Fragments arranged by size:
4594 1811 1336
SbfI CC_TGCA'GG
Cuts at: 5093 5093
Size: 7741
ScaI AGT'ACT
Cuts at: 2126 3444 5989 7701 2126
Size: 1318 2545 1712 2166
Fragments arranged by size:
2545 2166 1712 1318
SfcI C'TryA_G
Cuts at: 826 1045 2823 2942 3529 3813 3932 4206 5089
Size: 219 1778 119 587 284 119 274 883
Cuts at: 5089 6224 6905 7096 826
Size: 1135 681 191 1471
Fragments arranged by size:
1778 1471 1135 883 681 587 284 274
219 191 119 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7741
SgrAI Cr'CCGG_yG
Cuts at: 1700 2289 1700
Size: 589 7152
SmaI CCC'GGG
Cuts at: 1872 5068 1872
Size: 3196 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3491 4228 4977 5845 6746 7008
Size: 197 926 1548 737 749 868 901 262
Cuts at: 7008 820
Size: 1553
Fragments arranged by size:
1553 1548 926 901 868 749 737 262
197
SnaBI TAC'GTA
Cuts at: 493 4681 5532 493
Size: 4188 851 2702
Fragments arranged by size:
4188 2702 851
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7741
SphI G_CATG'C
Cuts at: 1132 3187 5099 1132
Size: 2055 1912 3774
Fragments arranged by size:
3774 2055 1912
SspI AAT'ATT
Cuts at: 5 52 5665 5
Size: 47 5613 2081
Fragments arranged by size:
5613 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7741
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 4033 5180 513
Size: 552 939 333 1696 1147 3074
Fragments arranged by size:
3074 1696 1147 939 552 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2246 4870 5768 5785 5938 6123
Size: 1255 447 219 2624 898 17 153 185
Cuts at: 6123 6568 325
Size: 445 1498
Fragments arranged by size:
2624 1498 1255 898 447 445 219 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3442 4941
Size: 274 80 33 51 1443 147 1318 1499
Cuts at: 4941 5987 7699 96
Size: 1046 1712 138
Fragments arranged by size:
1712 1499 1443 1318 1046 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2370 2402 7701 7733 2370
Size: 32 5299 32 2378
Fragments arranged by size:
5299 2378 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3785 1316
Size: 2469 5272
XbaI T'CTAG_A
Cuts at: 5077 5077
Size: 7741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2430 3022 4819 2372
Size: 58 592 1797 5294
Fragments arranged by size:
5294 1797 592 58
XhoI C'TCGA_G
Cuts at: 1943 4228 1943
Size: 2285 5456
XmaI C'CCGG_G
Cuts at: 1870 5066 1870
Size: 3196 4545
XmnI GAAnn'nnTTC
Cuts at: 5870 5870
Size: 7741
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5549 276
Size: 53 83 186 936 4015 2468
Fragments arranged by size:
4015 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI