Restriction map for
pFN21AB5599
(Circular) (Six-base) MAPSORT of: pFN21AB5599.seq Check: 3317 from: 1 to: 7501
pFN21AB5599 7501 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3070 4247 3070
Size: 1177 6324
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5311 278
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4844 1937
Size: 2907 4594
Acc65I G'GTAC_C
Cuts at: 2693 4822 2693
Size: 2129 5372
AclI AA'CG_TT
Cuts at: 1563 5628 6001 1563
Size: 4065 373 3063
Fragments arranged by size:
4065 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3800 3891 5564 6948 7216 1632
Size: 2168 91 1673 1384 268 1917
Fragments arranged by size:
2168 1917 1673 1384 268 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7304
AflIII A'CryG_T
Cuts at: 1203 6400 7377 1203
Size: 5197 977 1327
Fragments arranged by size:
5197 1327 977
AgeI A'CCGG_T
Cuts at: 6332 6332
Size: 7501
AhdI GACnn_n'nnGTC
Cuts at: 6230 6230
Size: 7501
AleI CACnn'nnGTG
Cuts at: 1166 2108 2345 1166
Size: 942 237 6322
Fragments arranged by size:
6322 942 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7501
AlwNI CAG_nnn'CTG
Cuts at: 2663 3937 4682 5244 6816 2663
Size: 1274 745 562 1572 3348
Fragments arranged by size:
3348 1572 1274 745 562
ApaLI G'TGCA_C
Cuts at: 5558 6714 5558
Size: 1156 6345
ApoI r'AATT_y
Cuts at: 1493 2309 2369 2708 3218 3298 4516 4525 4810
Size: 816 60 339 510 80 1218 9 285
Cuts at: 4810 5036 5188 7297 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1218 816 510 339 285 226
152 80 60 9
AseI AT'TA_AT
Cuts at: 160 1032 2619 6055 160
Size: 872 1587 3436 1606
Fragments arranged by size:
3436 1606 1587 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
AvaI C'yCGr_G
Cuts at: 1870 1943 4826 1870
Size: 73 2883 4545
Fragments arranged by size:
4545 2883 73
BamHI G'GATC_C
Cuts at: 4831 5198 4831
Size: 367 7134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2693 4822 6277 618
Size: 325 217 627 906 2129 1455 1842
Fragments arranged by size:
2129 1842 1455 906 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 3199 3648 4820 1391
Size: 742 1066 449 1172 4072
Fragments arranged by size:
4072 1172 1066 742 449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3290 3444 4497 928
Size: 971 1391 154 1053 3932
Fragments arranged by size:
3932 1391 1053 971 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5692 5726 1903
Size: 34 3755 34 3678
Fragments arranged by size:
3755 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4264 5393 6603 4264
Size: 1129 1210 5162
Fragments arranged by size:
5162 1210 1129
BclI T'GATC_A
Cuts at: 1548 2185 4495 1548
Size: 637 2310 4554
Fragments arranged by size:
4554 2310 637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6112 136
Size: 107 122 71 631 605 4440 1525
Fragments arranged by size:
4440 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7496 7496
Size: 7501
BlpI GC'TnA_GC
Cuts at: 4918 4918
Size: 7501
Bme1580I G_kGCm'C
Cuts at: 1790 2481 5562 6718 1790
Size: 691 3081 1156 2573
Fragments arranged by size:
3081 2573 1156 691
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3202 5232 6190
Size: 416 53 83 399 24 1785 2030 958
Cuts at: 6190 442
Size: 1753
Fragments arranged by size:
2030 1785 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2859 4203 6161 1239
Size: 1620 1344 1958 2579
Fragments arranged by size:
2579 1958 1620 1344
Bpu10I CC'TnA_GC
Cuts at: 3762 3762
Size: 7501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5626 6491 6789 5626
Size: 865 298 6338
Fragments arranged by size:
6338 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6164 882
Size: 617 4665 2219
Fragments arranged by size:
4665 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5292 493
Size: 711 4088 2702
Fragments arranged by size:
4088 2702 711
BsaBI GATnn'nnATC
Cuts at: 4392 4695 7495 4392
Size: 303 2800 4398
Fragments arranged by size:
4398 2800 303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5308 5690 6325 275
Size: 53 83 186 936 3775 382 635 1451
Fragments arranged by size:
3775 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5934 6332 6606 6753 7164 5934
Size: 398 274 147 411 6271
Fragments arranged by size:
6271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2832 2862 4213 4243 1178
Size: 30 165 30 1429 30 1351 30 4436
Fragments arranged by size:
4436 1429 1351 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 3713 4358 4367 1186
Size: 973 1554 645 9 4320
Fragments arranged by size:
4320 1554 973 645 9
BseYI C'CCAG_C
Cuts at: 2046 4710 6704 7200 2046
Size: 2664 1994 496 2347
Fragments arranged by size:
2664 2347 1994 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2709 1163
Size: 573 973 5955
Fragments arranged by size:
5955 973 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2023 2082 4951 5712 6740 1540
Size: 275 186 22 59 2869 761 1028 2301
Fragments arranged by size:
2869 2301 1028 761 275 186 59 22
BsiHKAI G_wGCw'C
Cuts at: 4368 5562 5647 6718 4368
Size: 1194 85 1071 5151
Fragments arranged by size:
5151 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 3037 4183 5012 5105 3037
Size: 1146 829 93 5433
Fragments arranged by size:
5433 1146 829 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2481 3199 3648 4368 4820 5562
Size: 399 343 348 718 449 720 452 742
Cuts at: 5562 5647 6718 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 718 452 449 399
348 343 85
BspEI T'CCGG_A
Cuts at: 7164 7164
Size: 7501
BspHI T'CATG_A
Cuts at: 3118 3364 5390 7120 3118
Size: 246 2026 1730 3499
Fragments arranged by size:
3499 2026 1730 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 3070 3112 4247 4856 844
Size: 1837 389 42 1135 609 3489
Fragments arranged by size:
3489 1837 1135 609 389 42
BsrBI CCG'CTC
Cuts at: 1394 1823 5388 1394
Size: 429 3565 3507
Fragments arranged by size:
3565 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5996 6170 65
Size: 1183 4748 174 1396
Fragments arranged by size:
4748 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2578 6145 6332 1700
Size: 110 205 563 3567 187 2869
Fragments arranged by size:
3567 2869 563 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 2678 3952 4643 96
Size: 2582 1274 691 2954
Fragments arranged by size:
2954 2582 1274 691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7501
BssSI C'ACGA_G
Cuts at: 3945 5561 6573 3945
Size: 1616 1012 4873
Fragments arranged by size:
4873 1616 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7501
BstBI TT'CG_AA
Cuts at: 6305 6305
Size: 7501
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7501
BstXI CCAn_nnnn'nTGG
Cuts at: 4181 4181
Size: 7501
BstYI r'GATC_y
Cuts at: 1972 3259 3600 4021 4287 4393 4831 5198 5583
Size: 1287 341 421 266 106 438 367 385
Cuts at: 5583 5600 7052 7138 7150 7496 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1287 438 421 385 367 346
341 266 106 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2071 487
Size: 683 56 845 5917
Fragments arranged by size:
5917 845 683 56
BtsI GCAGTG_nn'
Cuts at: 2388 2528 4113 4234 5017 5811 5831 2388
Size: 140 1585 121 783 794 20 4058
Fragments arranged by size:
4058 1585 794 783 140 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7501
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7466
DraI TTT'AAA
Cuts at: 2355 2625 3303 3406 4805 2355
Size: 270 678 103 1399 5051
Fragments arranged by size:
5051 1399 678 270 103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6508 809
Size: 507 5192 1802
Fragments arranged by size:
5192 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 2079 2100
Size: 54 1425 50 275 18 190 59 21
Cuts at: 2100 3750 4948 5837 7466 8
Size: 1650 1198 889 1629 43
Fragments arranged by size:
1650 1629 1425 1198 889 275 190 59
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2020 2079 4948 1537
Size: 275 208 59 2869 4090
Fragments arranged by size:
4090 2869 275 208 59
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 5431 1353
Size: 1647 2431 3423
Fragments arranged by size:
3423 2431 1647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6088 6462 6608 387
Size: 1064 138 4499 374 146 1280
Fragments arranged by size:
4499 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2859 3800 3891 4203 5564 6161 6948
Size: 393 1227 941 91 312 1361 597 787
Cuts at: 6948 7216 1239
Size: 268 1524
Fragments arranged by size:
1524 1361 1227 941 787 597 393 312
268 91
EcoO109I rG'GnC_Cy
Cuts at: 2034 2034
Size: 7501
EcoRI G'AATT_C
Cuts at: 4810 5188 4810
Size: 378 7123
FspI TGC'GCA
Cuts at: 6007 6007
Size: 7501
HaeII r_GCGC'y
Cuts at: 1678 6648 1678
Size: 4970 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3672 3704 4487 4519 4585
Size: 32 135 32 2100 32 783 32 66
Cuts at: 4585 4612 4617 4644 6142 6174 6216 6248 1373
Size: 27 5 27 1498 32 42 32 2626
Fragments arranged by size:
2626 2100 1498 783 135 66 42 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4845 5091 669
Size: 988 281 2907 246 3079
Fragments arranged by size:
3079 2907 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2156 748
Size: 1408 6093
HpaI GTT'AAC
Cuts at: 5091 5091
Size: 7501
KpnI G_GTAC'C
Cuts at: 2697 4826 2697
Size: 2129 5372
MfeI C'AATT_G
Cuts at: 5100 5100
Size: 7501
MluI A'CGCG_T
Cuts at: 7377 7377
Size: 7501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2848 3859 6615 6799 1305
Size: 1543 1011 2756 184 2007
Fragments arranged by size:
2756 2007 1543 1011 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3752 10
Size: 54 1425 343 1920 3759
Fragments arranged by size:
3759 1920 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2193 2345 2961 5459 5818 5977
Size: 648 942 85 152 616 2498 359 159
Cuts at: 5977 518
Size: 2042
Fragments arranged by size:
2498 2042 942 648 616 359 159 152
85
MspA1I CmG'CkG
Cuts at: 1606 3937 4917 5594 6742 6987 1606
Size: 2331 980 677 1148 245 2120
Fragments arranged by size:
2331 2120 1148 980 677 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 7501
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6949
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7501
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 7501
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7501
NotI GC'GGCC_GC
Cuts at: 4948 4948
Size: 7501
NspI r_CATG'y
Cuts at: 1132 1229 2216 3842 4150 4859 6404 1132
Size: 97 987 1626 308 709 1545 2229
Fragments arranged by size:
2229 1626 1545 987 709 308 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7501
PciI A'CATG_T
Cuts at: 6400 6400
Size: 7501
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3977 4076 1108
Size: 2869 99 4533
Fragments arranged by size:
4533 2869 99
PfoI T'CCnGG_A
Cuts at: 4341 4341
Size: 7501
PmeI GTTT'AAAC
Cuts at: 4805 4805
Size: 7501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5570 5602 7083 7115 3527
Size: 32 2011 32 1481 32 3913
Fragments arranged by size:
3913 2011 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2034 2034
Size: 7501
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7501
PsiI TTA'TAA
Cuts at: 5071 5071
Size: 7501
PstI C_TGCA'G
Cuts at: 830 4853 830
Size: 4023 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7501
PvuII CAG'CTG
Cuts at: 3937 3937
Size: 7501
SalI G'TCGA_C
Cuts at: 1936 4843 1936
Size: 2907 4594
SanDI GG'GwC_CC
Cuts at: 2034 2034
Size: 7501
SbfI CC_TGCA'GG
Cuts at: 4853 4853
Size: 7501
ScaI AGT'ACT
Cuts at: 2172 5749 7461 2172
Size: 3577 1712 2212
Fragments arranged by size:
3577 2212 1712
SfcI C'TryA_G
Cuts at: 826 1045 2248 3252 4731 4849 5984 6665 6856
Size: 219 1203 1004 1479 118 1135 681 191
Cuts at: 6856 826
Size: 1471
Fragments arranged by size:
1479 1471 1203 1135 1004 681 219 191
118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7501
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7501
SmaI CCC'GGG
Cuts at: 1872 4828 1872
Size: 2956 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5605 6506 6768 820
Size: 197 926 3662 901 262 1553
Fragments arranged by size:
3662 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5292 493
Size: 4799 2702
SpeI A'CTAG_T
Cuts at: 152 2065 152
Size: 1913 5588
SphI G_CATG'C
Cuts at: 1132 4859 1132
Size: 3727 3774
SspI AAT'ATT
Cuts at: 5 52 2745 2830 5425 5
Size: 47 2693 85 2595 2081
Fragments arranged by size:
2693 2595 2081 85 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7501
StyI C'CwwG_G
Cuts at: 513 1065 2301 4940 513
Size: 552 1236 2639 3074
Fragments arranged by size:
3074 2639 1236 552
SwaI ATTT'AAAT
Cuts at: 3303 3303
Size: 7501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 3984 4548 5528 5545 5698 5883
Size: 1255 882 1522 564 980 17 153 185
Cuts at: 5883 6328 325
Size: 445 1498
Fragments arranged by size:
1522 1498 1255 980 882 564 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2170 2678 3187
Size: 274 80 33 51 1443 193 508 509
Cuts at: 3187 3952 4643 5747 7459 96
Size: 765 691 1104 1712 138
Fragments arranged by size:
1712 1443 1104 765 691 509 508 274
193 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4155 4187 4213 4245 7461 7493 4155
Size: 32 26 32 3216 32 4163
Fragments arranged by size:
4163 3216 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7501
XbaI T'CTAG_A
Cuts at: 4837 4837
Size: 7501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3745 3974 3745
Size: 229 7272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7501
XmaI C'CCGG_G
Cuts at: 1870 4826 1870
Size: 2956 4545
XmnI GAAnn'nnTTC
Cuts at: 2311 3379 5630 2311
Size: 1068 2251 4182
Fragments arranged by size:
4182 2251 1068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5309 276
Size: 53 83 186 936 3775 2468
Fragments arranged by size:
3775 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI