Restriction map for
pFN21AB5593
(Circular) (Six-base) MAPSORT of: pFN21AB5593.seq Check: 7627 from: 1 to: 10294
pFN21AB5593 10294 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2534 8104 278
Size: 53 83 186 936 998 5570 2468
Fragments arranged by size:
5570 2468 998 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2538 4942 5726 7637 1937
Size: 601 2404 784 1911 4594
Fragments arranged by size:
4594 2404 1911 784 601
Acc65I G'GTAC_C
Cuts at: 4318 7615 4318
Size: 3297 6997
AclI AA'CG_TT
Cuts at: 1563 8421 8794 1563
Size: 6858 373 3063
Fragments arranged by size:
6858 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 3126 3264 4124 4257 6162 6539 6765
Size: 954 540 138 860 133 1905 377 226
Cuts at: 6765 7068 7270 8357 9741 10009 1632
Size: 303 202 1087 1384 268 1917
Fragments arranged by size:
1917 1905 1384 1087 954 860 540 377
303 268 226 202 138 133
AflII C'TTAA_G
Cuts at: 820 1017 3424 820
Size: 197 2407 7690
Fragments arranged by size:
7690 2407 197
AflIII A'CryG_T
Cuts at: 1203 2984 9193 10170 1203
Size: 1781 6209 977 1327
Fragments arranged by size:
6209 1781 1327 977
AgeI A'CCGG_T
Cuts at: 9125 9125
Size: 10294
AhdI GACnn_n'nnGTC
Cuts at: 9023 9023
Size: 10294
AleI CACnn'nnGTG
Cuts at: 1166 5859 1166
Size: 4693 5601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2608 3882 7458 1184
Size: 1424 1274 3576 4020
Fragments arranged by size:
4020 3576 1424 1274
AlwNI CAG_nnn'CTG
Cuts at: 3076 6113 6833 6851 7082 7552 8037 9609 3076
Size: 3037 720 18 231 470 485 1572 3761
Fragments arranged by size:
3761 3037 1572 720 485 470 231 18
ApaI G_GGCC'C
Cuts at: 7317 7317
Size: 10294
ApaLI G'TGCA_C
Cuts at: 8351 9507 8351
Size: 1156 9138
ApoI r'AATT_y
Cuts at: 1493 2138 2684 2912 4163 5779 6986 7603 7829
Size: 645 546 228 1251 1616 1207 617 226
Cuts at: 7829 7981 10090 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1616 1251 1207 645 617 546
228 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2049 5136 5178 8848 160
Size: 872 1017 3087 42 3670 1606
Fragments arranged by size:
3670 3087 1606 1017 872 42
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10294
AvaI C'yCGr_G
Cuts at: 1870 1943 7506 7619 1870
Size: 73 5563 113 4545
Fragments arranged by size:
5563 4545 113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7346 7379 7346
Size: 33 10261
BamHI G'GATC_C
Cuts at: 5944 7624 7991 5944
Size: 1680 367 8247
Fragments arranged by size:
8247 1680 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4318 7492 7615 9070 618
Size: 325 217 627 2531 3174 123 1455 1842
Fragments arranged by size:
3174 2531 1842 1455 627 325 217 123
BanII G_rGCy'C
Cuts at: 1391 5171 6588 6757 7317 7613 1391
Size: 3780 1417 169 560 296 4072
Fragments arranged by size:
4072 3780 1417 560 296 169
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3381 3840 4245 5288 5922 6150 6545
Size: 971 1482 459 405 1043 634 228 395
Cuts at: 6545 6753 928
Size: 208 4469
Fragments arranged by size:
4469 1482 1043 971 634 459 405 395
228 208
BbvCI CC'TCA_GC
Cuts at: 3836 3836
Size: 10294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8485 8519 1903
Size: 34 6548 34 3678
Fragments arranged by size:
6548 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2508 4506 6190 8186 9396 2508
Size: 1998 1684 1996 1210 3406
Fragments arranged by size:
3406 1998 1996 1684 1210
BclI T'GATC_A
Cuts at: 1548 2094 1548
Size: 546 9748
BfrBI ATG'CAT
Cuts at: 5429 5429
Size: 10294
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 8905 136
Size: 107 122 71 631 605 2313 4920 1525
Fragments arranged by size:
4920 2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3025 4249 4289 6673 7281 10289 3025
Size: 1224 40 2384 608 3008 3030
Fragments arranged by size:
3030 3008 2384 1224 608 40
BlpI GC'TnA_GC
Cuts at: 2941 5561 7711 2941
Size: 2620 2150 5524
Fragments arranged by size:
5524 2620 2150
Bme1580I G_kGCm'C
Cuts at: 1790 7317 8355 9511 1790
Size: 5527 1038 1156 2573
Fragments arranged by size:
5527 2573 1156 1038
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2721 7159 8025
Size: 416 53 83 399 24 1304 4438 866
Cuts at: 8025 8983 442
Size: 958 1753
Fragments arranged by size:
4438 1753 1304 958 866 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5886 1056
Size: 4830 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 4053 5155 6209 8954 1239
Size: 869 1945 1102 1054 2745 2579
Fragments arranged by size:
2745 2579 1945 1102 1054 869
Bpu10I CC'TnA_GC
Cuts at: 3836 5601 6151 3836
Size: 1765 550 7979
Fragments arranged by size:
7979 1765 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2616 5110 6341 6561 8419 9284 9582 2616
Size: 2494 1231 220 1858 865 298 3328
Fragments arranged by size:
3328 2494 1858 1231 865 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3517 8957 882
Size: 617 2018 5440 2219
Fragments arranged by size:
5440 2219 2018 617
BsaAI yAC'GTr
Cuts at: 493 1204 8085 493
Size: 711 6881 2702
Fragments arranged by size:
6881 2702 711
BsaBI GATnn'nnATC
Cuts at: 10288 10288
Size: 10294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2531 8101 8483 9118
Size: 53 83 186 936 998 5570 382 635
Cuts at: 9118 275
Size: 1451
Fragments arranged by size:
5570 1451 998 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8727 9125 9399 9546 9957 8727
Size: 398 274 147 411 9064
Fragments arranged by size:
9064 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3447 3477 4257 4287 7209
Size: 30 165 30 2044 30 780 30 2922
Cuts at: 7209 7239 1178
Size: 30 4233
Fragments arranged by size:
4233 2922 2044 780 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3853 5789 6596 6623 7230 1186
Size: 2667 1936 807 27 607 4250
Fragments arranged by size:
4250 2667 1936 807 607 27
BseYI C'CCAG_C
Cuts at: 3005 6872 9497 9993 3005
Size: 3867 2625 496 3306
Fragments arranged by size:
3867 3306 2625 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2781 4440 5923 7454 1163
Size: 573 1045 1659 1483 1531 4003
Fragments arranged by size:
4003 1659 1531 1483 1045 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7744 8505 9533 1540
Size: 275 186 5743 761 1028 2301
Fragments arranged by size:
5743 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5078 6588 8355 8440 9511 5078
Size: 1510 1767 85 1071 5861
Fragments arranged by size:
5861 1767 1510 1071 85
BsmI GAATG_Cn'
Cuts at: 3037 3138 5251 7805 7898 3037
Size: 101 2113 2554 93 5433
Fragments arranged by size:
5433 2554 2113 101 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2687 6142 2687
Size: 3455 6839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5078 5171 6588 6757 7317 7613 8355
Size: 399 3288 93 1417 169 560 296 742
Cuts at: 8355 8440 9511 1391
Size: 85 1071 2174
Fragments arranged by size:
3288 2174 1417 1071 742 560 399 296
169 93 85
BspEI T'CCGG_A
Cuts at: 9957 9957
Size: 10294
BspHI T'CATG_A
Cuts at: 3313 3661 8183 9913 3313
Size: 348 4522 1730 3694
Fragments arranged by size:
4522 3694 1730 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4033 6336 7649 844
Size: 3189 2303 1313 3489
Fragments arranged by size:
3489 3189 2303 1313
BsrBI CCG'CTC
Cuts at: 1394 1823 8181 1394
Size: 429 6358 3507
Fragments arranged by size:
6358 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6891 8789 8963 65
Size: 1183 5643 1898 174 1396
Fragments arranged by size:
5643 1898 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8938 9125 1700
Size: 110 7128 187 2869
Fragments arranged by size:
7128 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4762 6999 96
Size: 4666 2237 3391
Fragments arranged by size:
4666 3391 2237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10294
BssSI C'ACGA_G
Cuts at: 6768 6995 8354 9366 6768
Size: 227 1359 1012 7696
Fragments arranged by size:
7696 1359 1012 227
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2142 3076 6833 794
Size: 1348 934 3757 4255
Fragments arranged by size:
4255 3757 1348 934
BstBI TT'CG_AA
Cuts at: 9098 9098
Size: 10294
BstEII G'GTnAC_C
Cuts at: 1184 5606 7159 7501 1184
Size: 4422 1553 342 3977
Fragments arranged by size:
4422 3977 1553 342
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 6002 7446 7475 2086
Size: 3916 1444 29 4905
Fragments arranged by size:
4905 3916 1444 29
BstYI r'GATC_y
Cuts at: 1972 2552 3025 4249 4289 5944 6673 7281 7575
Size: 580 473 1224 40 1655 729 608 294
Cuts at: 7575 7624 7991 8376 8393 9845 9931 9943 10289
Size: 49 367 385 17 1452 86 12 346
Cuts at: 10289 1972
Size: 1977
Fragments arranged by size:
1977 1655 1452 1224 729 608 580 473
385 367 346 294 86 49 40 17
12
BstZ17I GTA'TAC
Cuts at: 4943 4943
Size: 10294
Bsu36I CC'TnA_GG
Cuts at: 3546 5277 3546
Size: 1731 8563
BtgI C'CryG_G
Cuts at: 513 1065 2004 6957 513
Size: 552 939 4953 3850
Fragments arranged by size:
4953 3850 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9555
Fragments arranged by size:
9555 683 56
BtsI GCAGTG_nn'
Cuts at: 2602 3949 4779 5911 7810 8604 8624 2602
Size: 1347 830 1132 1899 794 20 4272
Fragments arranged by size:
4272 1899 1347 1132 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2114 2149 6624 6659 529
Size: 35 1550 35 4475 35 4164
Fragments arranged by size:
4475 4164 1550 35 35 35
DraI TTT'AAA
Cuts at: 5113 7598 5113
Size: 2485 7809
DraIII CAC_nnn'GTG
Cuts at: 2118 2118
Size: 10294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9301 809
Size: 507 7985 1802
Fragments arranged by size:
7985 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3300 7741 8630
Size: 54 1425 50 275 18 1470 4441 889
Cuts at: 8630 10259 8
Size: 1629 43
Fragments arranged by size:
4441 1629 1470 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7741 1537
Size: 275 5929 4090
Fragments arranged by size:
5929 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2250 3942 4037 4194 4635 5608 5637
Size: 672 225 1692 95 157 441 973 29
Cuts at: 5637 5661 6069 6316 6618 6719 7044 8224 1353
Size: 24 408 247 302 101 325 1180 3423
Fragments arranged by size:
3423 1692 1180 973 672 441 408 325
302 247 225 157 101 95 29 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3013 8881 9255 9401 387
Size: 1064 138 1424 5868 374 146 1280
Fragments arranged by size:
5868 1424 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6586 6586
Size: 10294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2586 3126 3264 4053 4124 4257
Size: 393 476 478 540 138 789 71 133
Cuts at: 4257 5155 6162 6209 6539 6765 7068 7270 8357
Size: 898 1007 47 330 226 303 202 1087
Cuts at: 8357 8954 9741 10009 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1087 1007 898 789 787 597 540
478 476 393 330 303 268 226 202
138 133 71 47
EcoNI CCTnn'n_nnAGG
Cuts at: 3531 5861 3531
Size: 2330 7964
EcoO109I rG'GnC_Cy
Cuts at: 2174 4674 6325 7314 2174
Size: 2500 1651 989 5154
Fragments arranged by size:
5154 2500 1651 989
EcoRI G'AATT_C
Cuts at: 2912 7603 7981 2912
Size: 4691 378 5225
Fragments arranged by size:
5225 4691 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5576 5818 6327 5576
Size: 242 509 9543
Fragments arranged by size:
9543 509 242
FspI TGC'GCA
Cuts at: 8800 8800
Size: 10294
HaeII r_GCGC'y
Cuts at: 1678 4603 9441 1678
Size: 2925 4838 2531
Fragments arranged by size:
4838 2925 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 2506 2538 3194
Size: 32 135 32 655 32 247 32 656
Cuts at: 3194 3200 3226 3232 5636 5668 5744 5776 6716
Size: 6 26 6 2404 32 76 32 940
Cuts at: 6716 6748 7209 7241 7376 7408 8935 8967 9009
Size: 32 461 32 135 32 1527 32 42
Cuts at: 9009 9041 1373
Size: 32 2626
Fragments arranged by size:
2626 2404 1527 940 656 655 461 247
135 135 76 42 32 32 32 32
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2246 4110 4516 6943 7638 7884
Size: 988 281 308 1864 406 2427 695 246
Cuts at: 7884 669
Size: 3079
Fragments arranged by size:
3079 2427 1864 988 695 406 308 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10294
HpaI GTT'AAC
Cuts at: 4110 4516 7884 4110
Size: 406 3368 6520
Fragments arranged by size:
6520 3368 406
KpnI G_GTAC'C
Cuts at: 4322 7619 4322
Size: 3297 6997
MfeI C'AATT_G
Cuts at: 6815 7422 7473 7893 6815
Size: 607 51 420 9216
Fragments arranged by size:
9216 607 420 51
MluI A'CGCG_T
Cuts at: 10170 10170
Size: 10294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2491 5707 7500 9408 9592 1305
Size: 1186 3216 1793 1908 184 2007
Fragments arranged by size:
3216 2007 1908 1793 1186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3302 10
Size: 54 1425 343 1470 7002
Fragments arranged by size:
7002 1470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3864 4541 5859 6115 6962 7325 8252
Size: 648 2698 677 1318 256 847 363 927
Cuts at: 8252 8611 8770 518
Size: 359 159 2042
Fragments arranged by size:
2698 2042 1318 927 847 677 648 363
359 256 159
MspA1I CmG'CkG
Cuts at: 1606 2512 2765 6619 6848 7360 7710 8387 9535
Size: 906 253 3854 229 512 350 677 1148
Cuts at: 9535 9780 1606
Size: 245 2120
Fragments arranged by size:
3854 2120 1148 906 677 512 350 253
245 229
NcoI C'CATG_G
Cuts at: 513 1065 2004 6957 513
Size: 552 939 4953 3850
Fragments arranged by size:
4953 3850 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10294
NheI G'CTAG_C
Cuts at: 1052 5882 1052
Size: 4830 5464
NotI GC'GGCC_GC
Cuts at: 7741 7741
Size: 10294
NsiI A_TGCA'T
Cuts at: 5431 5431
Size: 10294
NspI r_CATG'y
Cuts at: 1132 1229 2149 2816 2988 6535 7330 7652 9197
Size: 97 920 667 172 3547 795 322 1545
Cuts at: 9197 1132
Size: 2229
Fragments arranged by size:
3547 2229 1545 920 795 667 322 172
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10294
PciI A'CATG_T
Cuts at: 2984 9193 2984
Size: 6209 4085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 7552 1108
Size: 1278 5166 3850
Fragments arranged by size:
5166 3850 1278
PfoI T'CCnGG_A
Cuts at: 4627 5299 4627
Size: 672 9622
PmeI GTTT'AAAC
Cuts at: 7598 7598
Size: 10294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8363 8395 9876 9908 8363
Size: 32 1481 32 8749
Fragments arranged by size:
8749 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4674 6325 4674
Size: 1651 8643
PshAI GACnn'nnGTC
Cuts at: 1613 2534 1613
Size: 921 9373
PsiI TTA'TAA
Cuts at: 7864 7864
Size: 10294
PspOMI G'GGCC_C
Cuts at: 7313 7313
Size: 10294
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3231 3263 3231
Size: 32 10262
PstI C_TGCA'G
Cuts at: 830 4786 5816 6448 7646 830
Size: 3956 1030 632 1198 3478
Fragments arranged by size:
3956 3478 1198 1030 632
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10294
PvuII CAG'CTG
Cuts at: 2765 6619 6848 7360 2765
Size: 3854 229 512 5699
Fragments arranged by size:
5699 3854 512 229
SacI G_AGCT'C
Cuts at: 6588 6588
Size: 10294
SalI G'TCGA_C
Cuts at: 1936 7636 1936
Size: 5700 4594
SapI GCTCTTCn'nnn_
Cuts at: 2025 2250 5661 6069 2025
Size: 225 3411 408 6250
Fragments arranged by size:
6250 3411 408 225
SbfI CC_TGCA'GG
Cuts at: 7646 7646
Size: 10294
ScaI AGT'ACT
Cuts at: 5155 8542 10254 5155
Size: 3387 1712 5195
Fragments arranged by size:
5195 3387 1712
SexAI A'CCwGG_T
Cuts at: 4120 4321 5820 4120
Size: 201 1499 8594
Fragments arranged by size:
8594 1499 201
SfcI C'TryA_G
Cuts at: 826 1045 2392 2417 4293 4782 5812 6444 7642
Size: 219 1347 25 1876 489 1030 632 1198
Cuts at: 7642 8777 9458 9649 826
Size: 1135 681 191 1471
Fragments arranged by size:
1876 1471 1347 1198 1135 1030 681 632
489 219 191 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10294
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10294
SmaI CCC'GGG
Cuts at: 1872 7621 1872
Size: 5749 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2631 3424 5125 6320 6576 8398
Size: 197 926 688 793 1701 1195 256 1822
Cuts at: 8398 9299 9561 820
Size: 901 262 1553
Fragments arranged by size:
1822 1701 1553 1195 926 901 793 688
262 256 197
SnaBI TAC'GTA
Cuts at: 493 8085 493
Size: 7592 2702
SpeI A'CTAG_T
Cuts at: 152 3877 5492 152
Size: 3725 1615 4954
Fragments arranged by size:
4954 3725 1615
SphI G_CATG'C
Cuts at: 1132 2149 7652 1132
Size: 1017 5503 3774
Fragments arranged by size:
5503 3774 1017
SspI AAT'ATT
Cuts at: 5 52 3269 3370 3384 3646 5134 5829 7112
Size: 47 3217 101 14 262 1488 695 1283
Cuts at: 7112 8218 5
Size: 1106 2081
Fragments arranged by size:
3217 2081 1488 1283 1106 695 262 101
47 14
StuI AGG'CCT
Cuts at: 1517 3537 6417 1517
Size: 2020 2880 5394
Fragments arranged by size:
5394 2880 2020
StyI C'CwwG_G
Cuts at: 513 1065 2004 2479 3116 4437 6665 6957 7243
Size: 552 939 475 637 1321 2228 292 286
Cuts at: 7243 7402 7733 513
Size: 159 331 3074
Fragments arranged by size:
3074 2228 1321 939 637 552 475 331
292 286 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5600 5954 6105 7147 7434 7504 8321
Size: 1255 4020 354 151 1042 287 70 817
Cuts at: 8321 8338 8491 8676 9121 325
Size: 17 153 185 445 1498
Fragments arranged by size:
4020 1498 1255 1042 817 445 354 287
185 153 151 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2035 3911 3966
Size: 274 80 33 51 1443 58 1876 55
Cuts at: 3966 4762 5066 5153 6999 8540 10252 96
Size: 796 304 87 1846 1541 1712 138
Fragments arranged by size:
1876 1846 1712 1541 1443 796 304 274
138 87 80 58 55 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4658 4690 5929 5961 10254 10286 4658
Size: 32 1239 32 4293 32 4666
Fragments arranged by size:
4666 4293 1239 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7165 1316
Size: 5849 4445
XbaI T'CTAG_A
Cuts at: 6676 7630 6676
Size: 954 9340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 7339 7339
Size: 10294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10294
XmaI C'CCGG_G
Cuts at: 1870 7619 1870
Size: 5749 4545
XmnI GAAnn'nnTTC
Cuts at: 3111 3342 4583 5205 5352 5670 8423 3111
Size: 231 1241 622 147 318 2753 4982
Fragments arranged by size:
4982 2753 1241 622 318 231 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2532 8102 276
Size: 53 83 186 936 998 5570 2468
Fragments arranged by size:
5570 2468 998 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsiWI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI RsrII SacII SanDI SfiI SfoI
SrfI SwaI