Restriction map for
pFN21AB5591
(Circular) (Six-base) MAPSORT of: pFN21AB5591.seq Check: 4670 from: 1 to: 7483
pFN21AB5591 7483 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 4137 4186 2599
Size: 1538 49 5896
Fragments arranged by size:
5896 1538 49
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5293 278
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3723 4826 1937
Size: 1786 1103 4594
Fragments arranged by size:
4594 1786 1103
Acc65I G'GTAC_C
Cuts at: 4804 4804
Size: 7483
AclI AA'CG_TT
Cuts at: 1563 5610 5983 1563
Size: 4047 373 3063
Fragments arranged by size:
4047 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 3452 5546 6930 7198 1632
Size: 831 989 2094 1384 268 1917
Fragments arranged by size:
2094 1917 1384 989 831 268
AflII C'TTAA_G
Cuts at: 820 1017 4736 820
Size: 197 3719 3567
Fragments arranged by size:
3719 3567 197
AflIII A'CryG_T
Cuts at: 1203 2665 6382 7359 1203
Size: 1462 3717 977 1327
Fragments arranged by size:
3717 1462 1327 977
AgeI A'CCGG_T
Cuts at: 6314 6314
Size: 7483
AhdI GACnn_n'nnGTC
Cuts at: 6212 6212
Size: 7483
AleI CACnn'nnGTG
Cuts at: 1166 3545 1166
Size: 2379 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7483
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4327 4359 4327
Size: 32 7451
AlwNI CAG_nnn'CTG
Cuts at: 2441 2578 4529 5226 6798 2441
Size: 137 1951 697 1572 3126
Fragments arranged by size:
3126 1951 1572 697 137
ApaLI G'TGCA_C
Cuts at: 5540 6696 5540
Size: 1156 6327
ApoI r'AATT_y
Cuts at: 1493 2101 4057 4792 5018 5170 7279 1493
Size: 608 1956 735 226 152 2109 1697
Fragments arranged by size:
2109 1956 1697 735 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6037 160
Size: 872 5005 1606
Fragments arranged by size:
5005 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
AvaI C'yCGr_G
Cuts at: 1870 1943 2350 3418 3956 4672 4808 1870
Size: 73 407 1068 538 716 136 4545
Fragments arranged by size:
4545 1068 716 538 407 136 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3238 3271 3238
Size: 33 7450
BamHI G'GATC_C
Cuts at: 2733 4576 4813 5180 2733
Size: 1843 237 367 5036
Fragments arranged by size:
5036 1843 367 237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4532 4804 6259 618
Size: 325 217 627 2745 272 1455 1842
Fragments arranged by size:
2745 1842 1455 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2357 3276 3389 3523 3966 4610 4685 4725
Size: 966 919 113 134 443 644 75 40
Cuts at: 4725 4802 1391
Size: 77 4072
Fragments arranged by size:
4072 966 919 644 443 134 113 77
75 40
BbeI G_GCGC'C
Cuts at: 4536 4536
Size: 7483
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5674 5708 1903
Size: 34 3737 34 3678
Fragments arranged by size:
3737 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2520 5375 6585 2520
Size: 2855 1210 3418
Fragments arranged by size:
3418 2855 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3420 3597 6094
Size: 107 122 71 631 605 1748 177 2497
Cuts at: 6094 136
Size: 1525
Fragments arranged by size:
2497 1748 1525 631 605 177 122 107
71
BglII A'GATC_T
Cuts at: 2424 3351 3475 7478 2424
Size: 927 124 4003 2429
Fragments arranged by size:
4003 2429 927 124
BlpI GC'TnA_GC
Cuts at: 4900 4900
Size: 7483
Bme1580I G_kGCm'C
Cuts at: 1790 5544 6700 1790
Size: 3754 1156 2573
Fragments arranged by size:
3754 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 3616 4622
Size: 416 53 83 399 24 1967 232 1006
Cuts at: 4622 5214 6172 442
Size: 592 958 1753
Fragments arranged by size:
1967 1753 1006 958 592 416 399 232
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 2975 3290 3403 3785 4006 4415 4519
Size: 1373 363 315 113 382 221 409 104
Cuts at: 4519 4724 6143 1239
Size: 205 1419 2579
Fragments arranged by size:
2579 1419 1373 409 382 363 315 221
205 113 104
Bpu10I CC'TnA_GC
Cuts at: 4679 4679
Size: 7483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5608 6473 6771 5608
Size: 865 298 6320
Fragments arranged by size:
6320 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6146 882
Size: 617 4647 2219
Fragments arranged by size:
4647 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5274 493
Size: 711 4070 2702
Fragments arranged by size:
4070 2702 711
BsaBI GATnn'nnATC
Cuts at: 2561 7477 2561
Size: 4916 2567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4533 4617 5290 5672
Size: 53 83 186 936 3000 84 673 382
Cuts at: 5672 6307 275
Size: 635 1451
Fragments arranged by size:
3000 1451 936 673 635 382 186 84
83 53
BsaWI w'CCGG_w
Cuts at: 3999 4035 4346 5916 6314 6588 6735 7146 3999
Size: 36 311 1570 398 274 147 411 4336
Fragments arranged by size:
4336 1570 411 398 311 274 147 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 3283 3313 3376
Size: 30 165 30 735 30 1115 30 63
Cuts at: 3376 3406 3722 3752 4145 4175 4211 4241 1178
Size: 30 316 30 393 30 36 30 4420
Fragments arranged by size:
4420 1115 735 393 316 165 63 36
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2338 3473 3543 3881 3974 1186
Size: 1152 1135 70 338 93 4695
Fragments arranged by size:
4695 1152 1135 338 93 70
BseYI C'CCAG_C
Cuts at: 3316 3447 3633 3772 3814 4289 6686 7182 3316
Size: 131 186 139 42 475 2397 496 3617
Fragments arranged by size:
3617 2397 496 475 186 139 131 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 3414 4642 4659 4678 1163
Size: 573 776 902 1228 17 19 3968
Fragments arranged by size:
3968 1228 902 776 573 19 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4180 4545 4933 5694 6722
Size: 275 186 2035 144 365 388 761 1028
Cuts at: 6722 1540
Size: 2301
Fragments arranged by size:
2301 2035 1028 761 388 365 275 186
144
BsiHKAI G_wGCw'C
Cuts at: 3276 4610 4685 4725 5544 5629 6700 3276
Size: 1334 75 40 819 85 1071 4059
Fragments arranged by size:
4059 1334 1071 819 85 75 40
BsmI GAATG_Cn'
Cuts at: 2658 4994 5087 2658
Size: 2336 93 5054
Fragments arranged by size:
5054 2336 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3803 3803
Size: 7483
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2357 3276 3389 3523 3966 4610 4685
Size: 399 567 919 113 134 443 644 75
Cuts at: 4685 4725 4802 5544 5629 6700 1391
Size: 40 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 644 567 443 399
134 113 85 77 75 40
BspEI T'CCGG_A
Cuts at: 3999 4346 7146 3999
Size: 347 2800 4336
Fragments arranged by size:
4336 2800 347
BspHI T'CATG_A
Cuts at: 5372 7102 5372
Size: 1730 5753
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 3009 4137 4186 4838 844
Size: 1755 410 1128 49 652 3489
Fragments arranged by size:
3489 1755 1128 652 410 49
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 5370 1394
Size: 429 1594 1953 3507
Fragments arranged by size:
3507 1953 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2842 3336 5978 6152 65
Size: 1183 1594 494 2642 174 1396
Fragments arranged by size:
2642 1594 1396 1183 494 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 3129 3742 6127 6314 1700
Size: 110 1121 198 613 2385 187 2869
Fragments arranged by size:
2869 2385 1121 613 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 4566 96
Size: 4470 3013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7483
BssSI C'ACGA_G
Cuts at: 5543 6555 5543
Size: 1012 6471
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4201 794
Size: 3407 4076
BstBI TT'CG_AA
Cuts at: 3651 6287 3651
Size: 2636 4847
BstEII G'GTnAC_C
Cuts at: 1184 2135 1184
Size: 951 6532
BstXI CCAn_nnnn'nTGG
Cuts at: 3316 3316
Size: 7483
BstYI r'GATC_y
Cuts at: 1972 2424 2733 3351 3475 4576 4813 5180 5565
Size: 452 309 618 124 1101 237 367 385
Cuts at: 5565 5582 7034 7120 7132 7478 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1101 618 452 385 367 346
309 237 124 86 17 12
BstZ17I GTA'TAC
Cuts at: 3724 3724
Size: 7483
Bsu36I CC'TnA_GG
Cuts at: 3441 4522 3441
Size: 1081 6402
BtgI C'CryG_G
Cuts at: 513 1065 2004 2938 3523 3801 4170 4180 513
Size: 552 939 934 585 278 369 10 3816
Fragments arranged by size:
3816 939 934 585 552 369 278 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2311 3013 4582 487
Size: 683 56 1085 702 1569 3388
Fragments arranged by size:
3388 1569 1085 702 683 56
BtsI GCAGTG_nn'
Cuts at: 3206 3540 4434 4999 5793 5813 3206
Size: 334 894 565 794 20 4876
Fragments arranged by size:
4876 894 794 565 334 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7483
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2299 2334 2502 2537 2674 2709 3162
Size: 35 1735 35 168 35 137 35 453
Cuts at: 3162 3197 529
Size: 35 4815
Fragments arranged by size:
4815 1735 453 168 137 35 35 35
35 35
DraI TTT'AAA
Cuts at: 4787 4787
Size: 7483
DraIII CAC_nnn'GTG
Cuts at: 2062 3879 2062
Size: 1817 5666
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6490 809
Size: 507 5174 1802
Fragments arranged by size:
5174 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2544 2769 2860
Size: 54 1425 50 275 18 714 225 91
Cuts at: 2860 3024 4177 4930 5819 7448 8
Size: 164 1153 753 889 1629 43
Fragments arranged by size:
1629 1425 1153 889 753 714 275 225
164 91 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4177 4930 1537
Size: 275 2365 753 4090
Fragments arranged by size:
4090 2365 753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3281 3367 3824 3831 3858 5413 1353
Size: 1928 86 457 7 27 1555 3423
Fragments arranged by size:
3423 1928 1555 457 86 27 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3221 3346 4291 6070 6444 6590
Size: 1064 138 1632 125 945 1779 374 146
Cuts at: 6590 387
Size: 1280
Fragments arranged by size:
1779 1632 1280 1064 945 374 146 138
125
EcoICRI GAG'CTC
Cuts at: 3274 4608 4683 4723 3274
Size: 1334 75 40 6034
Fragments arranged by size:
6034 1334 75 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2612 2975 3290 3403 3452 3785
Size: 393 831 149 363 315 113 49 333
Cuts at: 3785 4006 4415 4519 4724 5546 6143 6930 7198
Size: 221 409 104 205 822 597 787 268
Cuts at: 7198 1239
Size: 1524
Fragments arranged by size:
1524 831 822 787 597 409 393 363
333 315 268 221 205 149 113 104
49
EcoO109I rG'GnC_Cy
Cuts at: 3622 3630 3818 3622
Size: 8 188 7287
Fragments arranged by size:
7287 188 8
EcoRI G'AATT_C
Cuts at: 2101 4792 5170 2101
Size: 2691 378 4414
Fragments arranged by size:
4414 2691 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2797 2278
Size: 519 6964
FspI TGC'GCA
Cuts at: 3569 5989 3569
Size: 2420 5063
HaeII r_GCGC'y
Cuts at: 1678 4193 4536 6630 1678
Size: 2515 343 2094 2531
Fragments arranged by size:
2531 2515 2094 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2276 2308 2768
Size: 32 135 32 566 32 106 32 460
Cuts at: 2768 2800 3522 3554 3722 3754 6124 6156 6198
Size: 32 722 32 168 32 2370 32 42
Cuts at: 6198 6230 1373
Size: 32 2626
Fragments arranged by size:
2626 2370 722 566 460 168 135 106
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4827 5073 669
Size: 988 281 2889 246 3079
Fragments arranged by size:
3079 2889 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7483
HpaI GTT'AAC
Cuts at: 5073 5073
Size: 7483
KasI G'GCGC_C
Cuts at: 4532 4532
Size: 7483
KpnI G_GTAC'C
Cuts at: 4808 4808
Size: 7483
MfeI C'AATT_G
Cuts at: 3937 5082 3937
Size: 1145 6338
MluI A'CGCG_T
Cuts at: 7359 7359
Size: 7483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 3000 3333 3953 6597 6781 1305
Size: 1101 594 333 620 2644 184 2007
Fragments arranged by size:
2644 2007 1101 620 594 333 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2546 2771 10
Size: 54 1425 343 714 225 4722
Fragments arranged by size:
4722 1425 714 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2759 2765 2943 3545 4601 5441
Size: 648 843 750 6 178 602 1056 840
Cuts at: 5441 5800 5959 518
Size: 359 159 2042
Fragments arranged by size:
2042 1056 843 840 750 648 602 359
178 159 6
MspA1I CmG'CkG
Cuts at: 1606 3525 4182 4899 5576 6724 6969 1606
Size: 1919 657 717 677 1148 245 2120
Fragments arranged by size:
2120 1919 1148 717 677 657 245
NaeI GCC'GGC
Cuts at: 2933 3744 2933
Size: 811 6672
NarI GG'CG_CC
Cuts at: 4533 4533
Size: 7483
NcoI C'CATG_G
Cuts at: 513 1065 2004 2938 513
Size: 552 939 934 5058
Fragments arranged by size:
5058 939 934 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7483
NgoMIV G'CCGG_C
Cuts at: 2931 3742 2931
Size: 811 6672
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7483
NotI GC'GGCC_GC
Cuts at: 4177 4930 4177
Size: 753 6730
NspI r_CATG'y
Cuts at: 1132 1229 2669 3316 3664 4841 6386 1132
Size: 97 1440 647 348 1177 1545 2229
Fragments arranged by size:
2229 1545 1440 1177 647 348 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7483
PciI A'CATG_T
Cuts at: 2665 6382 2665
Size: 3717 3766
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 1108
Size: 1561 5922
PmeI GTTT'AAAC
Cuts at: 4787 4787
Size: 7483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5552 5584 7065 7097 5552
Size: 32 1481 32 5938
Fragments arranged by size:
5938 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3622 3630 3818 3622
Size: 8 188 7287
Fragments arranged by size:
7287 188 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7483
PsiI TTA'TAA
Cuts at: 5053 5053
Size: 7483
PspXI vC'TCGA_Gb
Cuts at: 3956 3956
Size: 7483
PstI C_TGCA'G
Cuts at: 830 2574 3896 4074 4197 4835 830
Size: 1744 1322 178 123 638 3478
Fragments arranged by size:
3478 1744 1322 638 178 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7483
SacI G_AGCT'C
Cuts at: 3276 4610 4685 4725 3276
Size: 1334 75 40 6034
Fragments arranged by size:
6034 1334 75 40
SacII CC_GC'GG
Cuts at: 3526 4183 3526
Size: 657 6826
SalI G'TCGA_C
Cuts at: 1936 4825 1936
Size: 2889 4594
SanDI GG'GwC_CC
Cuts at: 3818 3818
Size: 7483
SapI GCTCTTCn'nnn_
Cuts at: 3281 3824 3281
Size: 543 6940
SbfI CC_TGCA'GG
Cuts at: 4835 4835
Size: 7483
ScaI AGT'ACT
Cuts at: 2690 5731 7443 2690
Size: 3041 1712 2730
Fragments arranged by size:
3041 2730 1712
SexAI A'CCwGG_T
Cuts at: 2799 2799
Size: 7483
SfcI C'TryA_G
Cuts at: 826 1045 2570 3586 3892 4070 4193 4831 5966
Size: 219 1525 1016 306 178 123 638 1135
Cuts at: 5966 6647 6838 826
Size: 681 191 1471
Fragments arranged by size:
1525 1471 1135 1016 681 638 306 219
191 178 123
SfoI GGC'GCC
Cuts at: 4534 4534
Size: 7483
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7483
SgrAI Cr'CCGG_yG
Cuts at: 1700 3742 1700
Size: 2042 5441
SmaI CCC'GGG
Cuts at: 1872 4810 1872
Size: 2938 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3956 4736 5587 6488 6750 820
Size: 197 926 2013 780 851 901 262 1553
Fragments arranged by size:
2013 1553 926 901 851 780 262 197
SnaBI TAC'GTA
Cuts at: 493 5274 493
Size: 4781 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7483
SphI G_CATG'C
Cuts at: 1132 4841 1132
Size: 3709 3774
SspI AAT'ATT
Cuts at: 5 52 5407 5
Size: 47 5355 2081
Fragments arranged by size:
5355 2081 47
StuI AGG'CCT
Cuts at: 1517 3333 4644 1517
Size: 1816 1311 4356
Fragments arranged by size:
4356 1816 1311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2772 2938 4922 513
Size: 552 939 768 166 1984 3074
Fragments arranged by size:
3074 1984 939 768 552 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3502 4559 5510 5527 5680 5865
Size: 1255 1803 119 1057 951 17 153 185
Cuts at: 5865 6310 325
Size: 445 1498
Fragments arranged by size:
1803 1498 1255 1057 951 445 185 153
119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2688 4566 5729
Size: 274 80 33 51 1443 711 1878 1163
Cuts at: 5729 7441 96
Size: 1712 138
Fragments arranged by size:
1878 1712 1443 1163 711 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3283 3315 3376 3408 3810 3842 4561 4593 4602
Size: 32 61 32 402 32 719 32 9
Cuts at: 4602 4634 7443 7475 3283
Size: 32 2809 32 3291
Fragments arranged by size:
3291 2809 719 402 61 32 32 32
32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7483
XbaI T'CTAG_A
Cuts at: 4819 4819
Size: 7483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 4297 4609 2554
Size: 1743 312 5428
Fragments arranged by size:
5428 1743 312
XhoI C'TCGA_G
Cuts at: 1943 3956 1943
Size: 2013 5470
XmaI C'CCGG_G
Cuts at: 1870 4808 1870
Size: 2938 4545
XmnI GAAnn'nnTTC
Cuts at: 3615 5612 3615
Size: 1997 5486
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5291 276
Size: 53 83 186 936 3757 2468
Fragments arranged by size:
3757 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PsrI PvuII RsrII SfiI SrfI SwaI