Restriction map for
pFN21AB5590
(Circular) (Six-base) MAPSORT of: pFN21AB5590.seq Check: 6370 from: 1 to: 5752
pFN21AB5590 5752 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3815 5199 5467 1632
Size: 2183 1384 268 1917
Fragments arranged by size:
2183 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2562 4651 5628 1203
Size: 1359 2089 977 1327
Fragments arranged by size:
2089 1359 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2614 2646 2614
Size: 32 5720
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaI G_GGCC'C
Cuts at: 2027 2027
Size: 5752
ApaLI G'TGCA_C
Cuts at: 2206 2287 2374 3809 4965 2206
Size: 81 87 1435 1156 2993
Fragments arranged by size:
2993 1435 1156 87 81
ApoI r'AATT_y
Cuts at: 1493 2849 3061 3287 3439 5548 1493
Size: 1356 212 226 152 2109 1697
Fragments arranged by size:
2109 1697 1356 226 212 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2455 2474 2661 3077 1870
Size: 73 512 19 187 416 4545
Fragments arranged by size:
4545 512 416 187 73 19
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2122 3073 4528 618
Size: 325 217 627 319 16 951 1455 1842
Fragments arranged by size:
1842 1455 951 627 325 319 217 16
BanII G_rGCy'C
Cuts at: 1391 2027 2511 3071 1391
Size: 636 484 560 4072
Fragments arranged by size:
4072 636 560 484
BbeI G_GCGC'C
Cuts at: 2126 2126
Size: 5752
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 2840 928
Size: 971 666 275 3840
Fragments arranged by size:
3840 971 666 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 2543 2931
Size: 107 122 71 631 605 467 404 388
Cuts at: 2931 4363 136
Size: 1432 1525
Fragments arranged by size:
1525 1432 631 605 467 404 388 122
107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2111 2210 2291 2378 2927 3813 4969
Size: 237 84 99 81 87 549 886 1156
Cuts at: 4969 1790
Size: 2573
Fragments arranged by size:
2573 1156 886 549 237 99 87 84
81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4412 1239
Size: 3173 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2379 3543 493
Size: 711 1175 1164 2702
Fragments arranged by size:
2702 1175 1164 711
BsaBI GATnn'nnATC
Cuts at: 2651 2960 5746 2651
Size: 309 2786 2657
Fragments arranged by size:
2786 2657 309
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3559 3941 4576
Size: 53 83 186 936 590 1436 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1436 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 2483 2513 2614
Size: 30 165 30 840 30 210 30 101
Cuts at: 2614 2644 2666 2696 2906 2936 1178
Size: 30 22 30 210 30 3994
Fragments arranged by size:
3994 840 210 210 165 101 30 30
30 30 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2473 2504 1186
Size: 856 431 31 4434
Fragments arranged by size:
4434 856 431 31
BseYI C'CCAG_C
Cuts at: 2194 2435 4955 5451 2194
Size: 241 2520 496 2495
Fragments arranged by size:
2520 2495 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2434 2806 2866 1163
Size: 573 698 372 60 4049
Fragments arranged by size:
4049 698 573 372 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2072 2076 2875 3202 3963 4991
Size: 275 186 71 4 799 327 761 1028
Cuts at: 4991 1540
Size: 2301
Fragments arranged by size:
2301 1028 799 761 327 275 186 71
4
BsiHKAI G_wGCw'C
Cuts at: 2210 2291 2308 2378 3813 3898 4969 2210
Size: 81 17 70 1435 85 1071 2993
Fragments arranged by size:
2993 1435 1071 85 81 70 17
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2111 2210 2291 2308 2378 2511
Size: 399 237 84 99 81 17 70 133
Cuts at: 2511 2927 3071 3813 3898 4969 1391
Size: 416 144 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 237 144 133
99 85 84 81 70 17
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 2940 3641 5371 2940
Size: 701 1730 3321
Fragments arranged by size:
3321 1730 701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2071 2103 2119 2908 4396 4583
Size: 110 257 4 32 16 789 1488 187
Cuts at: 4583 1700
Size: 2869
Fragments arranged by size:
2869 1488 789 257 187 110 32 16
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 2150 3812 4824 2150
Size: 1662 1012 3078
Fragments arranged by size:
3078 1662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2769 794
Size: 1975 3777
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 2665 1184
Size: 1481 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2602 2194
Size: 408 5344
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2523 513
Size: 552 1458 3742
Fragments arranged by size:
3742 1458 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2623 2658 529
Size: 35 2059 35 3623
Fragments arranged by size:
3623 2059 35 35
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2619 2619
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2936 4759 809
Size: 507 1620 1823 1802
Fragments arranged by size:
1823 1802 1620 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2069 2073 2080
Size: 54 1425 50 275 18 239 4 7
Cuts at: 2080 2265 2872 3199 4088 5717 8
Size: 185 607 327 889 1629 43
Fragments arranged by size:
1629 1425 889 607 327 275 239 185
54 50 43 18 7 4
EagI C'GGCC_G
Cuts at: 1537 1812 2069 2073 2872 3199 1537
Size: 275 257 4 799 327 4090
Fragments arranged by size:
4090 799 327 275 257 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2739 3682 1353
Size: 896 490 943 3423
Fragments arranged by size:
3423 943 896 490
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3815 4412 5199 5467 1239
Size: 393 2183 597 787 268 1524
Fragments arranged by size:
2183 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2455 2686 2455
Size: 231 5521
EcoO109I rG'GnC_Cy
Cuts at: 2024 2239 2024
Size: 215 5537
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5752
FseI GG_CCGG'CC
Cuts at: 2075 2075
Size: 5752
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2126 2628 4899 1678
Size: 448 502 2271 2531
Fragments arranged by size:
2531 2271 502 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2666 2698 4393
Size: 32 135 32 671 32 391 32 1695
Cuts at: 4393 4425 4467 4499 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 671 391 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KasI G'GCGC_C
Cuts at: 2122 2122
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 3710 4069 4228 518
Size: 648 1662 882 359 159 2042
Fragments arranged by size:
2042 1662 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2194 2340 2361 3168 3845 4993 5238
Size: 488 100 146 21 807 677 1148 245
Cuts at: 5238 1606
Size: 2120
Fragments arranged by size:
2120 1148 807 677 488 245 146 100
21
NaeI GCC'GGC
Cuts at: 2069 2073 2121 2069
Size: 4 48 5700
Fragments arranged by size:
5700 48 4
NarI GG'CG_CC
Cuts at: 2123 2123
Size: 5752
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5200
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NgoMIV G'CCGG_C
Cuts at: 2067 2071 2119 2067
Size: 4 48 5700
Fragments arranged by size:
5700 48 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2407 3110 4655 1132
Size: 97 1178 703 1545 2229
Fragments arranged by size:
2229 1545 1178 703 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 2619 1108
Size: 1416 95 4241
Fragments arranged by size:
4241 1416 95
PfoI T'CCnGG_A
Cuts at: 2992 2992
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PmlI CAC'GTG
Cuts at: 2379 2379
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2646 3821 3853 5334 5366 2614
Size: 32 1175 32 1481 32 3000
Fragments arranged by size:
3000 1481 1175 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2427 1613
Size: 814 4938
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2023 2023
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2066 2093 2690 3104 830
Size: 1236 27 597 414 3478
Fragments arranged by size:
3478 1236 597 414 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2194 2361 2194
Size: 167 5585
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 2690 3104 2690
Size: 414 5338
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2062 2089 2686 3025 3100 4235 4916
Size: 219 1017 27 597 339 75 1135 681
Cuts at: 4916 5107 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1017 681 597 339 219 191
75 27
SfoI GGC'GCC
Cuts at: 2124 2124
Size: 5752
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2476 2663 3079 1872
Size: 604 187 416 4545
Fragments arranged by size:
4545 604 416 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 2407 3110 1132
Size: 1275 703 3774
Fragments arranged by size:
3774 1275 703
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2725 1517
Size: 1208 4544
StyI C'CwwG_G
Cuts at: 513 1065 2511 3191 513
Size: 552 1446 680 3074
Fragments arranged by size:
3074 1446 680 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 3779 3796 3949 4134 4579 325
Size: 1255 905 1294 17 153 185 445 1498
Fragments arranged by size:
1498 1294 1255 905 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3021 3998 5710
Size: 274 80 33 51 1443 1044 977 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1712 1443 1044 977 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 2906 2938 5712 5744 2483
Size: 32 391 32 2774 32 2491
Fragments arranged by size:
2774 2491 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2908 2525
Size: 383 5369
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2474 2661 3077 1870
Size: 604 187 416 4545
Fragments arranged by size:
4545 604 416 187
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI
NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI