Restriction map for
pFN21AB5585
(Circular) (Six-base) MAPSORT of: pFN21AB5585.seq Check: 4217 from: 1 to: 5680
pFN21AB5585 5680 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3743 5127 5395 1632
Size: 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 2826 4579 5556 1203
Size: 1623 1753 977 1327
Fragments arranged by size:
1753 1623 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2176 3423 4995 2176
Size: 1247 1572 2861
Fragments arranged by size:
2861 1572 1247
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2033 2395 2768 2989 3215 3367 5476 1493
Size: 540 362 373 221 226 152 2109 1697
Fragments arranged by size:
2109 1697 540 373 362 226 221 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3001 4456 618
Size: 325 217 627 1214 1455 1842
Fragments arranged by size:
1842 1455 1214 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 928
Size: 971 405 4304
Fragments arranged by size:
4304 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 2364 1548
Size: 816 4864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4897 1790
Size: 1951 1156 2573
Fragments arranged by size:
2573 1951 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 3411 4369
Size: 416 53 83 399 24 747 1247 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1247 958 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2571 1056
Size: 1515 4165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4340 1239
Size: 3101 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 2111 5674 2111
Size: 3563 2117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2446 3487 3869 4504
Size: 53 83 186 936 913 1041 382 635
Cuts at: 4504 275
Size: 1451
Fragments arranged by size:
1451 1041 936 913 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5680
BseYI C'CCAG_C
Cuts at: 4883 5379 4883
Size: 496 5184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 2850 3191 3284 2850
Size: 341 93 5246
Fragments arranged by size:
5246 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2999 3741 3826 4897 1391
Size: 399 1209 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2771 794
Size: 1977 3703
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2275 3010 3377 3762 3779 5231 5317 5329
Size: 303 735 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 735 385 367 346 303 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3530
Fragments arranged by size:
3530 939 659 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2789 487
Size: 683 56 1563 3378
Fragments arranged by size:
3378 1563 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2553 3196 3990 4010 2142
Size: 411 643 794 20 3812
Fragments arranged by size:
3812 794 643 411 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2719 2754 529
Size: 35 2155 35 3455
Fragments arranged by size:
3455 2155 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2484 3127 4016
Size: 54 1425 50 275 18 654 643 889
Cuts at: 4016 5645 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 654 643 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 2941 3610 1353
Size: 956 632 669 3423
Fragments arranged by size:
3423 956 669 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3743 4340 5127 5395 1239
Size: 393 2111 597 787 268 1524
Fragments arranged by size:
2111 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2285 2862 2285
Size: 577 5103
EcoRI G'AATT_C
Cuts at: 2033 2989 3367 2033
Size: 956 378 4346
Fragments arranged by size:
4346 956 378
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 2603 2635 4321
Size: 32 135 32 705 32 294 32 1686
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1686 705 294 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2023 4794 4978 1305
Size: 718 2771 184 2007
Fragments arranged by size:
2771 2007 718 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 10
Size: 54 1425 343 654 3204
Fragments arranged by size:
3204 1425 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 3638 3997 4156 518
Size: 648 1711 761 359 159 2042
Fragments arranged by size:
2042 1711 761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2019 3096 3773 4921 5166 1606
Size: 413 1077 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 677 413 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3530
Fragments arranged by size:
3530 939 659 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 2567 1052
Size: 1515 4165
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2830 3038 4583 1132
Size: 97 1601 208 1545 2229
Fragments arranged by size:
2229 1601 1545 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 2826 4579 2826
Size: 1753 3927
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2285 2862 2285
Size: 577 5103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2767 3032 830
Size: 1937 265 3478
Fragments arranged by size:
3478 1937 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2763 3028 4163 4844 5035 826
Size: 219 1718 265 1135 681 191 1471
Fragments arranged by size:
1718 1471 1135 681 265 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 2266 152
Size: 2114 3566
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2663 3119 513
Size: 552 939 21 638 456 3074
Fragments arranged by size:
3074 939 638 552 456 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3707 3724 3877 4062 4507 325
Size: 1255 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2263 2341 2493
Size: 274 80 33 51 1443 286 78 152
Cuts at: 2493 2778 3926 5638 96
Size: 285 1148 1712 138
Fragments arranged by size:
1712 1443 1148 286 285 274 152 138
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI