Restriction map for
pFN21AB5584
(Circular) (Six-base) MAPSORT of: pFN21AB5584.seq Check: 9562 from: 1 to: 6628
pFN21AB5584 6628 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 3621 3949 3621
Size: 328 6300
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3086 4691 6075 6343 1632
Size: 1454 1605 1384 268 1917
Fragments arranged by size:
1917 1605 1454 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 2233 5527 6504 1203
Size: 1030 3294 977 1327
Fragments arranged by size:
3294 1327 1030 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6628
AlwNI CAG_nnn'CTG
Cuts at: 4371 5943 4371
Size: 1572 5056
ApaLI G'TGCA_C
Cuts at: 4685 5841 4685
Size: 1156 5472
ApoI r'AATT_y
Cuts at: 1493 2334 2400 2412 2729 2779 2944 3008 3937
Size: 841 66 12 317 50 165 64 929
Cuts at: 3937 4163 4315 6424 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 841 317 226 165 152
66 64 50 12
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 3953 1870
Size: 73 2010 4545
Fragments arranged by size:
4545 2010 73
BamHI G'GATC_C
Cuts at: 3958 4325 3958
Size: 367 6261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3621 3949 5404 618
Size: 325 217 627 1834 328 1455 1842
Fragments arranged by size:
1842 1834 1455 627 328 325 217
BanII G_rGCy'C
Cuts at: 1391 3947 1391
Size: 2556 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 2763 928
Size: 971 364 500 4793
Fragments arranged by size:
4793 971 500 364
BbvCI CC'TCA_GC
Cuts at: 3041 3041
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2692 4520 5730 2037
Size: 655 1828 1210 2935
Fragments arranged by size:
2935 1828 1210 655
BclI T'GATC_A
Cuts at: 1548 2344 1548
Size: 796 5832
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5239 136
Size: 107 122 71 631 605 3567 1525
Fragments arranged by size:
3567 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6623 6623
Size: 6628
BlpI GC'TnA_GC
Cuts at: 4045 4045
Size: 6628
Bme1580I G_kGCm'C
Cuts at: 1790 3360 4689 5845 1790
Size: 1570 1329 1156 2573
Fragments arranged by size:
2573 1570 1329 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 2980 3856
Size: 416 53 83 399 24 1514 49 876
Cuts at: 3856 4359 5317 442
Size: 503 958 1753
Fragments arranged by size:
1753 1514 958 876 503 416 399 83
53 49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3741 5288 1239
Size: 2502 1547 2579
Fragments arranged by size:
2579 2502 1547
Bpu10I CC'TnA_GC
Cuts at: 3041 3041
Size: 6628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 4753 5618 5916 2116
Size: 2637 865 298 2828
Fragments arranged by size:
2828 2637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2508 5291 882
Size: 617 1009 2783 2219
Fragments arranged by size:
2783 2219 1009 617
BsaAI yAC'GTr
Cuts at: 493 1204 2507 3479 4419 493
Size: 711 1303 972 940 2702
Fragments arranged by size:
2702 1303 972 940 711
BsaBI GATnn'nnATC
Cuts at: 6622 6622
Size: 6628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4817 5452 275
Size: 53 83 186 936 2902 382 635 1451
Fragments arranged by size:
2902 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3818 5061 5459 5733 5880 6291 3818
Size: 1243 398 274 147 411 4155
Fragments arranged by size:
4155 1243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6403
Fragments arranged by size:
6403 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 1186
Size: 1684 4944
BseYI C'CCAG_C
Cuts at: 2014 2976 3162 3395 3666 5831 6327 2014
Size: 962 186 233 271 2165 496 2315
Fragments arranged by size:
2315 2165 962 496 271 233 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3567 1163
Size: 573 1831 4224
Fragments arranged by size:
4224 1831 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4078 4839 5867 1540
Size: 275 186 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4689 4774 5845 4689
Size: 85 1071 5472
Fragments arranged by size:
5472 1071 85
BsmI GAATG_Cn'
Cuts at: 2162 2997 4139 4232 2162
Size: 835 1142 93 4558
Fragments arranged by size:
4558 1142 835 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3360 3947 4689 4774 5845 1391
Size: 399 1570 587 742 85 1071 2174
Fragments arranged by size:
2174 1570 1071 742 587 399 85
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 2347 2722 3487 4517 6247 2347
Size: 375 765 1030 1730 2728
Fragments arranged by size:
2728 1730 1030 765 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 3983 844
Size: 2134 1005 3489
Fragments arranged by size:
3489 2134 1005
BsrBI CCG'CTC
Cuts at: 1394 1823 4515 1394
Size: 429 2692 3507
Fragments arranged by size:
3507 2692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2943 5123 5297 65
Size: 1183 1695 2180 174 1396
Fragments arranged by size:
2180 1695 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 5272 5459 1700
Size: 110 433 3029 187 2869
Fragments arranged by size:
3029 2869 433 187 110
BsrGI T'GTAC_A
Cuts at: 96 3763 96
Size: 3667 2961
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 3318 4688 5700 3318
Size: 1370 1012 4246
Fragments arranged by size:
4246 1370 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3473 794
Size: 2679 3949
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstYI r'GATC_y
Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623
Size: 1986 367 385 17 1452 86 12 346
Cuts at: 6623 1972
Size: 1977
Fragments arranged by size:
1986 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2281 513
Size: 552 1216 4860
Fragments arranged by size:
4860 1216 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5889
Fragments arranged by size:
5889 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2883 3555 3665 4144 4938 4958 2468
Size: 415 672 110 479 794 20 4138
Fragments arranged by size:
4138 794 672 479 415 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DraIII CAC_nnn'GTG
Cuts at: 2151 3850 2151
Size: 1699 4929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5635 809
Size: 507 4319 1802
Fragments arranged by size:
4319 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3681 4075 4964
Size: 54 1425 50 275 18 1851 394 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1851 1629 1425 889 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2188 2304 4558 1353
Size: 835 116 2254 3423
Fragments arranged by size:
3423 2254 835 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5215 5589 5735 387
Size: 1064 138 3626 374 146 1280
Fragments arranged by size:
3626 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3086 3741 4691 5288 6075 6343 1239
Size: 393 1454 655 950 597 787 268 1524
Fragments arranged by size:
1524 1454 950 787 655 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3058 3521 3058
Size: 463 6165
EcoRI G'AATT_C
Cuts at: 2729 2779 3937 4315 2729
Size: 50 1158 378 5042
Fragments arranged by size:
5042 1158 378 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2780 2780
Size: 6628
FspI TGC'GCA
Cuts at: 5134 5134
Size: 6628
HaeII r_GCGC'y
Cuts at: 1678 3163 5775 1678
Size: 1485 2612 2531
Fragments arranged by size:
2612 2531 1485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 5269 5301 5343
Size: 32 135 32 463 32 3202 32 42
Cuts at: 5343 5375 1373
Size: 32 2626
Fragments arranged by size:
3202 2626 463 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2806 3972 4218 669
Size: 988 281 868 1166 246 3079
Fragments arranged by size:
3079 1166 988 868 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 3625 3953 3625
Size: 328 6300
MfeI C'AATT_G
Cuts at: 2882 4227 2882
Size: 1345 5283
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5742 5926 1305
Size: 4437 184 2007
Fragments arranged by size:
4437 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3683 10
Size: 54 1425 343 1851 2955
Fragments arranged by size:
2955 1851 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2599 3759 4586 4945 5104 518
Size: 648 1072 361 1160 827 359 159 2042
Fragments arranged by size:
2042 1160 1072 827 648 361 359 159
MspA1I CmG'CkG
Cuts at: 1606 4044 4721 5869 6114 1606
Size: 2438 677 1148 245 2120
Fragments arranged by size:
2438 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2281 513
Size: 552 1216 4860
Fragments arranged by size:
4860 1216 552
NdeI CA'TA_TG
Cuts at: 387 2596 3024 3496 387
Size: 2209 428 472 3519
Fragments arranged by size:
3519 2209 472 428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2237 2576 3986 5531 1132
Size: 97 1008 339 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 1008 339 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6628
PciI A'CATG_T
Cuts at: 2233 5527 2233
Size: 3294 3334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6628
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3058 3521 3058
Size: 463 6165
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 6596
PstI C_TGCA'G
Cuts at: 830 2989 3980 830
Size: 2159 991 3478
Fragments arranged by size:
3478 2159 991
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SfcI C'TryA_G
Cuts at: 826 1045 2587 2985 3291 3976 5111 5792 5983
Size: 219 1542 398 306 685 1135 681 191
Cuts at: 5983 826
Size: 1471
Fragments arranged by size:
1542 1471 1135 685 681 398 306 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 4732 5633 5895 820
Size: 197 926 152 2637 901 262 1553
Fragments arranged by size:
2637 1553 926 901 262 197 152
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 3800 4552 5
Size: 47 3748 752 2081
Fragments arranged by size:
3748 2081 752 47
StuI AGG'CCT
Cuts at: 1517 2357 2826 1517
Size: 840 469 5319
Fragments arranged by size:
5319 840 469
StyI C'CwwG_G
Cuts at: 513 1065 2281 2358 2930 4067 513
Size: 552 1216 77 572 1137 3074
Fragments arranged by size:
3074 1216 1137 572 552 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2589 2663 3838 4655 4672 4825 5010
Size: 1255 1009 74 1175 817 17 153 185
Cuts at: 5010 5455 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1175 1009 817 445 185 153
74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3763 4874 6586
Size: 274 80 33 51 1443 1786 1111 1712
Cuts at: 6586 96
Size: 138
Fragments arranged by size:
1786 1712 1443 1111 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3628 3660 6588 6620 3628
Size: 32 2928 32 3636
Fragments arranged by size:
3636 2928 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6628
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 4757 4757
Size: 6628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI