Restriction map for
pFN21AB5580
(Circular) (Six-base) MAPSORT of: pFN21AB5580.seq Check: 1542 from: 1 to: 6550
pFN21AB5580 6550 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3420 3420
Size: 6550
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2385 4360 278
Size: 53 83 186 936 849 1975 2468
Fragments arranged by size:
2468 1975 936 849 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 3893 1937
Size: 1176 780 4594
Fragments arranged by size:
4594 1176 780
Acc65I G'GTAC_C
Cuts at: 3871 3871
Size: 6550
AclI AA'CG_TT
Cuts at: 1563 4677 5050 1563
Size: 3114 373 3063
Fragments arranged by size:
3114 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 4613 5997 6265 1632
Size: 1302 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 1302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6353
AflIII A'CryG_T
Cuts at: 1203 3157 5449 6426 1203
Size: 1954 2292 977 1327
Fragments arranged by size:
2292 1954 1327 977
AgeI A'CCGG_T
Cuts at: 5381 5381
Size: 6550
AhdI GACnn_n'nnGTC
Cuts at: 5279 5279
Size: 6550
AleI CACnn'nnGTG
Cuts at: 1166 2180 2741 1166
Size: 1014 561 4975
Fragments arranged by size:
4975 1014 561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6550
AlwNI CAG_nnn'CTG
Cuts at: 4293 5865 4293
Size: 1572 4978
ApaLI G'TGCA_C
Cuts at: 3209 4607 5763 3209
Size: 1398 1156 3996
Fragments arranged by size:
3996 1398 1156
ApoI r'AATT_y
Cuts at: 1493 2503 3608 3859 4085 4237 6346 1493
Size: 1010 1105 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 1010 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5104 160
Size: 872 4072 1606
Fragments arranged by size:
4072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3875 1870
Size: 73 1048 884 4545
Fragments arranged by size:
4545 1048 884 73
BamHI G'GATC_C
Cuts at: 2195 2328 3687 3880 4247 2195
Size: 133 1359 193 367 4498
Fragments arranged by size:
4498 1359 367 193 133
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3871 5326 618
Size: 325 217 627 2084 1455 1842
Fragments arranged by size:
2084 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2881 3265 3339 3725 3869 1391
Size: 690 800 384 74 386 144 4072
Fragments arranged by size:
4072 800 690 386 384 144 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2381 2768 3672 928
Size: 971 482 387 904 3806
Fragments arranged by size:
3806 971 904 482 387
BbvCI CC'TCA_GC
Cuts at: 2064 2064
Size: 6550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3245 3279 4741 4775 1903
Size: 34 1308 34 1462 34 3678
Fragments arranged by size:
3678 1462 1308 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4442 5652 4442
Size: 1210 5340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6550
BfrBI ATG'CAT
Cuts at: 3539 3539
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3284 5161 136
Size: 107 122 71 631 605 1612 1877 1525
Fragments arranged by size:
1877 1612 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6545 6545
Size: 6550
BlpI GC'TnA_GC
Cuts at: 3967 3967
Size: 6550
Bme1580I G_kGCm'C
Cuts at: 1790 2888 3213 3367 4611 5767 1790
Size: 1098 325 154 1244 1156 2573
Fragments arranged by size:
2573 1244 1156 1098 325 154
BmgBI CAC'GTC
Cuts at: 1318 3094 3160 1318
Size: 1776 66 4708
Fragments arranged by size:
4708 1776 66
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2253 2378 3177
Size: 416 53 83 399 24 836 125 799
Cuts at: 3177 3268 3352 4281 5239 442
Size: 91 84 929 958 1753
Fragments arranged by size:
1753 958 929 836 799 416 399 125
91 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5210 1239
Size: 3971 2579
Bpu10I CC'TnA_GC
Cuts at: 2064 3007 2064
Size: 943 5607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3785 4675 5540 5838 3785
Size: 890 865 298 4497
Fragments arranged by size:
4497 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3099 3761 5213 882
Size: 617 1600 662 1452 2219
Fragments arranged by size:
2219 1600 1452 662 617
BsaAI yAC'GTr
Cuts at: 493 1204 2822 4341 493
Size: 711 1618 1519 2702
Fragments arranged by size:
2702 1618 1519 711
BsaBI GATnn'nnATC
Cuts at: 6544 6544
Size: 6550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2382 4357 4739
Size: 53 83 186 936 654 195 1975 382
Cuts at: 4739 5374 275
Size: 635 1451
Fragments arranged by size:
1975 1451 936 654 635 382 195 186
83 53
BsaWI w'CCGG_w
Cuts at: 2192 2556 2700 3450 3836 4983 5381 5655 5802
Size: 364 144 750 386 1147 398 274 147
Cuts at: 5802 6213 2192
Size: 411 2529
Fragments arranged by size:
2529 1147 750 411 398 386 364 274
147 144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 5281
Fragments arranged by size:
5281 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3028 3093 1186
Size: 1842 65 4643
Fragments arranged by size:
4643 1842 65
BseYI C'CCAG_C
Cuts at: 3274 5753 6249 3274
Size: 2479 496 3575
Fragments arranged by size:
3575 2479 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 1163
Size: 573 906 5071
Fragments arranged by size:
5071 906 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2440 4000 4761 5789 1540
Size: 275 186 439 1560 761 1028 2301
Fragments arranged by size:
2301 1560 1028 761 439 275 186
BsiHKAI G_wGCw'C
Cuts at: 2881 3213 3339 3474 3725 4611 4696 5767 2881
Size: 332 126 135 251 886 85 1071 3664
Fragments arranged by size:
3664 1071 886 332 251 135 126 85
BsmI GAATG_Cn'
Cuts at: 2070 4061 4154 2070
Size: 1991 93 4466
Fragments arranged by size:
4466 1991 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2881 2888 3213 3265 3339 3367
Size: 399 291 800 7 325 52 74 28
Cuts at: 3367 3474 3725 3869 4611 4696 5767 1391
Size: 107 251 144 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 399 325 291 251
144 107 85 74 52 28 7
BspEI T'CCGG_A
Cuts at: 2192 2700 6213 2192
Size: 508 3513 2529
Fragments arranged by size:
3513 2529 508
BspHI T'CATG_A
Cuts at: 3372 4439 6169 3372
Size: 1067 1730 3753
Fragments arranged by size:
3753 1730 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 3420 3905 844
Size: 1251 1325 485 3489
Fragments arranged by size:
3489 1325 1251 485
BsrBI CCG'CTC
Cuts at: 1394 1823 4437 1394
Size: 429 2614 3507
Fragments arranged by size:
3507 2614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3772 5045 5219 65
Size: 1183 2524 1273 174 1396
Fragments arranged by size:
2524 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2577 3703 5194 5381 1700
Size: 110 767 1126 1491 187 2869
Fragments arranged by size:
2869 1491 1126 767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6550
BssSI C'ACGA_G
Cuts at: 4610 5622 4610
Size: 1012 5538
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6550
BstBI TT'CG_AA
Cuts at: 5354 5354
Size: 6550
BstEII G'GTnAC_C
Cuts at: 1184 3446 1184
Size: 2262 4288
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 3391 2743
Size: 648 5902
BstYI r'GATC_y
Cuts at: 1972 2195 2328 3687 3880 4247 4632 4649 6101
Size: 223 133 1359 193 367 385 17 1452
Cuts at: 6101 6187 6199 6545 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1359 385 367 346 223 193
133 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2847 513
Size: 552 1782 4216
Fragments arranged by size:
4216 1782 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5811
Fragments arranged by size:
5811 683 56
BtsI GCAGTG_nn'
Cuts at: 2187 4066 4860 4880 2187
Size: 1879 794 20 3857
Fragments arranged by size:
3857 1879 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6515
DraI TTT'AAA
Cuts at: 3854 3854
Size: 6550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2315 2772 3158 5557 809
Size: 507 999 457 386 2399 1802
Fragments arranged by size:
2399 1802 999 507 457 386
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2437 2844 3826
Size: 54 1425 50 275 18 607 407 982
Cuts at: 3826 3997 4886 6515 8
Size: 171 889 1629 43
Fragments arranged by size:
1629 1425 982 889 607 407 275 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2437 3997 1537
Size: 275 625 1560 4090
Fragments arranged by size:
4090 1560 625 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3046 3344 4480 1353
Size: 1693 298 1136 3423
Fragments arranged by size:
3423 1693 1136 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5137 5511 5657 387
Size: 1064 138 3548 374 146 1280
Fragments arranged by size:
3548 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2879 3337 3723 2879
Size: 458 386 5706
Fragments arranged by size:
5706 458 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2934 4613 5210 5997 6265 1239
Size: 393 1302 1679 597 787 268 1524
Fragments arranged by size:
1679 1524 1302 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3137 3137
Size: 6550
EcoRI G'AATT_C
Cuts at: 3859 4237 3859
Size: 378 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2946 3455 2946
Size: 509 6041
FspI TGC'GCA
Cuts at: 5056 5056
Size: 6550
HaeII r_GCGC'y
Cuts at: 1678 2283 5697 1678
Size: 605 3414 2531
Fragments arranged by size:
3414 2531 605
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2336 2368 2417
Size: 32 135 32 576 32 156 32 49
Cuts at: 2417 2449 2716 2748 2957 2989 3023 3055 3072
Size: 32 267 32 209 32 34 32 17
Cuts at: 3072 3104 3215 3247 5191 5223 5265 5297 1373
Size: 32 111 32 1944 32 42 32 2626
Fragments arranged by size:
2626 1944 576 267 209 156 135 111
49 42 34 32 32 32 32 32
32 32 32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3406 3894 4140 669
Size: 988 281 1176 292 488 246 3079
Fragments arranged by size:
3079 1176 988 488 292 281 246
HindIII A'AGCT_T
Cuts at: 748 3049 748
Size: 2301 4249
HpaI GTT'AAC
Cuts at: 4140 4140
Size: 6550
KpnI G_GTAC'C
Cuts at: 3875 3875
Size: 6550
MfeI C'AATT_G
Cuts at: 2668 4149 2668
Size: 1481 5069
MluI A'CGCG_T
Cuts at: 6426 6426
Size: 6550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3750 5664 5848 1305
Size: 2445 1914 184 2007
Fragments arranged by size:
2445 2007 1914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2846 3828 10
Size: 54 1425 343 1014 982 2732
Fragments arranged by size:
2732 1425 1014 982 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2609 2741 4508 4867 5026 518
Size: 648 1014 429 132 1767 359 159 2042
Fragments arranged by size:
2042 1767 1014 648 429 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 2389 2517 2565 3966 4643 5791 6036 1606
Size: 783 128 48 1401 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 783 677 245 128 48
NaeI GCC'GGC
Cuts at: 2579 3705 2579
Size: 1126 5424
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5998
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6550
NgoMIV G'CCGG_C
Cuts at: 2577 3703 2577
Size: 1126 5424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6550
NotI GC'GGCC_GC
Cuts at: 3997 3997
Size: 6550
NsiI A_TGCA'T
Cuts at: 3541 3541
Size: 6550
NspI r_CATG'y
Cuts at: 1132 1229 3908 5453 1132
Size: 97 2679 1545 2229
Fragments arranged by size:
2679 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6550
PciI A'CATG_T
Cuts at: 5449 5449
Size: 6550
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6550
PfoI T'CCnGG_A
Cuts at: 3426 3426
Size: 6550
PmeI GTTT'AAAC
Cuts at: 3854 3854
Size: 6550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4619 4651 6132 6164 4619
Size: 32 1481 32 5005
Fragments arranged by size:
5005 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2381 1613
Size: 768 5782
PsiI TTA'TAA
Cuts at: 4120 4120
Size: 6550
PstI C_TGCA'G
Cuts at: 830 3417 3489 3902 830
Size: 2587 72 413 3478
Fragments arranged by size:
3478 2587 413 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6550
PvuII CAG'CTG
Cuts at: 2517 2517
Size: 6550
SacI G_AGCT'C
Cuts at: 2881 3339 3725 2881
Size: 458 386 5706
Fragments arranged by size:
5706 458 386
SalI G'TCGA_C
Cuts at: 1936 3112 3892 1936
Size: 1176 780 4594
Fragments arranged by size:
4594 1176 780
SapI GCTCTTCn'nnn_
Cuts at: 3344 3344
Size: 6550
SbfI CC_TGCA'GG
Cuts at: 3902 3902
Size: 6550
ScaI AGT'ACT
Cuts at: 2408 4798 6510 2408
Size: 2390 1712 2448
Fragments arranged by size:
2448 2390 1712
SfcI C'TryA_G
Cuts at: 826 1045 2052 2512 3413 3485 3552 3898 5033
Size: 219 1007 460 901 72 67 346 1135
Cuts at: 5033 5714 5905 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1007 901 681 460 346 219
191 72 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6550
SmaI CCC'GGG
Cuts at: 1872 3877 1872
Size: 2005 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3800 4654 5555 5817 820
Size: 197 926 1857 854 901 262 1553
Fragments arranged by size:
1857 1553 926 901 854 262 197
SnaBI TAC'GTA
Cuts at: 493 4341 493
Size: 3848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6550
SphI G_CATG'C
Cuts at: 1132 3908 1132
Size: 2776 3774
SspI AAT'ATT
Cuts at: 5 52 4474 5
Size: 47 4422 2081
Fragments arranged by size:
4422 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6550
StyI C'CwwG_G
Cuts at: 513 1065 3989 513
Size: 552 2924 3074
Fragments arranged by size:
3074 2924 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3133 3255 4577 4594 4747 4932 5377
Size: 1255 1553 122 1322 17 153 185 445
Cuts at: 5377 325
Size: 1498
Fragments arranged by size:
1553 1498 1322 1255 445 185 153 122
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 4796 6508
Size: 274 80 33 51 1443 429 2390 1712
Cuts at: 6508 96
Size: 138
Fragments arranged by size:
2390 1712 1443 429 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6510 6542 6510
Size: 32 6518
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6550
XbaI T'CTAG_A
Cuts at: 3886 3886
Size: 6550
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6550
XmaI C'CCGG_G
Cuts at: 1870 3875 1870
Size: 2005 4545
XmnI GAAnn'nnTTC
Cuts at: 2639 4679 2639
Size: 2040 4510
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2383 4358 276
Size: 53 83 186 936 849 1975 2468
Fragments arranged by size:
2468 1975 936 849 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI