Restriction map for
pFN21AB5574
(Circular) (Six-base) MAPSORT of: pFN21AB5574.seq Check: 5783 from: 1 to: 5341
pFN21AB5574 5341 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2256 2684 1937
Size: 319 428 4594
Fragments arranged by size:
4594 428 319
Acc65I G'GTAC_C
Cuts at: 2662 2662
Size: 5341
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 3404 4788 5056 1632
Size: 995 777 1384 268 1917
Fragments arranged by size:
1917 1384 995 777 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 2552 3084 4656 2552
Size: 532 1572 3237
Fragments arranged by size:
3237 1572 532
ApaLI G'TGCA_C
Cuts at: 2120 3398 4554 2120
Size: 1278 1156 2907
Fragments arranged by size:
2907 1278 1156
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 2182 2666 1870
Size: 73 219 20 484 4545
Fragments arranged by size:
4545 484 219 73 20
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2662 4117 618
Size: 325 217 627 875 1455 1842
Fragments arranged by size:
1842 1455 875 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2660 1391
Size: 1269 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4246
Fragments arranged by size:
4246 971 124
BbvCI CC'TCA_GC
Cuts at: 2196 2196
Size: 5341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2124 3402 4558 1790
Size: 334 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 334
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 4030 442
Size: 416 53 83 399 24 1655 958 1753
Fragments arranged by size:
1753 1655 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2255 4001 1239
Size: 934 82 1746 2579
Fragments arranged by size:
2579 1746 934 82
Bpu10I CC'TnA_GC
Cuts at: 2196 2196
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3466 4331 4629 2513
Size: 953 865 298 3225
Fragments arranged by size:
3225 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4004 882
Size: 617 2505 2219
Fragments arranged by size:
2505 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 2361 3132 493
Size: 711 915 242 771 2702
Fragments arranged by size:
2702 915 771 711 242
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2188 3148 3530
Size: 53 83 186 936 519 136 960 382
Cuts at: 3530 4165 275
Size: 635 1451
Fragments arranged by size:
1451 960 936 635 519 382 186 136
83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5116
Fragments arranged by size:
5116 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2014 1186
Size: 828 4513
BseYI C'CCAG_C
Cuts at: 2104 2249 2427 4544 5040 2104
Size: 145 178 2117 496 2405
Fragments arranged by size:
2405 2117 496 178 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2565 1163
Size: 573 528 301 3939
Fragments arranged by size:
3939 573 528 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2599 2791 3552 4580 1540
Size: 275 186 598 192 761 1028 2301
Fragments arranged by size:
2301 1028 761 598 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 2124 3402 3487 4558 2124
Size: 1278 85 1071 2907
Fragments arranged by size:
2907 1278 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
BsmBI CGTCTCn'nnnn_
Cuts at: 2497 2497
Size: 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2660 3402 3487 4558 1391
Size: 399 334 536 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 334 85
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2696 844
Size: 1852 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 2562 3228 1394
Size: 429 241 498 666 3507
Fragments arranged by size:
3507 666 498 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2176 3985 4172 1700
Size: 110 331 35 1809 187 2869
Fragments arranged by size:
2869 1809 331 187 110 35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 2137 2157 1925
Size: 212 20 5109
Fragments arranged by size:
5109 212 20
BssSI C'ACGA_G
Cuts at: 2450 3401 4413 2450
Size: 951 1012 3378
Fragments arranged by size:
3378 1012 951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5341
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 2308 1184
Size: 1124 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2104
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336
Size: 699 367 385 17 1452 86 12 346
Cuts at: 5336 1972
Size: 1977
Fragments arranged by size:
1977 1452 699 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 2394 2406 2436 513
Size: 552 939 324 66 12 30 3418
Fragments arranged by size:
3418 939 552 324 66 30 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2241 487
Size: 683 56 1015 3587
Fragments arranged by size:
3587 1015 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3651 3671 2857
Size: 794 20 4527
Fragments arranged by size:
4527 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4348 809
Size: 507 3032 1802
Fragments arranged by size:
3032 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2335 2391
Size: 54 1425 50 275 18 495 10 56
Cuts at: 2391 2403 2596 2788 3677 5306 8
Size: 12 193 192 889 1629 43
Fragments arranged by size:
1629 1425 889 495 275 193 192 56
54 50 43 18 12 10
EagI C'GGCC_G
Cuts at: 1537 1812 2596 2788 1537
Size: 275 784 192 4090
Fragments arranged by size:
4090 784 275 192
EarI CTCTTCn'nnn_
Cuts at: 1353 3271 1353
Size: 1918 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2526 3928 4302 4448 387
Size: 1064 138 937 1402 374 146 1280
Fragments arranged by size:
1402 1280 1064 937 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2255 2627 3404 4001 4788 5056
Size: 393 541 82 372 777 597 787 268
Cuts at: 5056 1239
Size: 1524
Fragments arranged by size:
1524 787 777 597 541 393 372 268
82
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 5341
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 5341
FspI TGC'GCA
Cuts at: 2477 3847 2477
Size: 1370 3971
HaeII r_GCGC'y
Cuts at: 1678 2023 2105 4488 1678
Size: 345 82 2383 2531
Fragments arranged by size:
2531 2383 345 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2666 2666
Size: 5341
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 10
Size: 54 1425 343 505 3014
Fragments arranged by size:
3014 1425 505 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3299 3658 3817 518
Size: 648 843 1290 359 159 2042
Fragments arranged by size:
2042 1290 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 2396 2757 3434 4582 4827 1606
Size: 672 118 361 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 361 245 118
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 5341
NcoI C'CATG_G
Cuts at: 513 1065 2004 2436 513
Size: 552 939 432 3418
Fragments arranged by size:
3418 939 552 432
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 3877
PfoI T'CCnGG_A
Cuts at: 2150 2150
Size: 5341
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PmlI CAC'GTG
Cuts at: 2361 2361
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2522 1613
Size: 909 4432
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2295 2353 2591 2693 830
Size: 1465 58 238 102 3478
Fragments arranged by size:
3478 1465 238 102 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 5341
SacII CC_GC'GG
Cuts at: 2397 2397
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 3589 5301 3589
Size: 1712 3629
SfcI C'TryA_G
Cuts at: 826 1045 2291 2349 2587 2689 3824 4505 4696
Size: 219 1246 58 238 102 1135 681 191
Cuts at: 4696 826
Size: 1471
Fragments arranged by size:
1471 1246 1135 681 238 219 191 102
58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2184 2668 1872
Size: 312 484 4545
Fragments arranged by size:
4545 484 312
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 3445 4346 4608 820
Size: 197 926 585 917 901 262 1553
Fragments arranged by size:
1553 926 917 901 585 262 197
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2436 2780 513
Size: 552 939 93 339 344 3074
Fragments arranged by size:
3074 939 552 344 339 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 2327 3368 3385 3538 3723 4168
Size: 1255 437 310 1041 17 153 185 445
Cuts at: 4168 325
Size: 1498
Fragments arranged by size:
1498 1255 1041 445 437 310 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3587 5299 96
Size: 274 80 33 51 1443 1610 1712 138
Fragments arranged by size:
1712 1610 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5341
XmaI C'CCGG_G
Cuts at: 1870 2182 2666 1870
Size: 312 484 4545
Fragments arranged by size:
4545 484 312
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI