Restriction map for
pFN21AB5573
(Circular) (Six-base) MAPSORT of: pFN21AB5573.seq Check: 1178 from: 1 to: 8320
pFN21AB5573 8320 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2227 3583 2227
Size: 1356 6964
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6130 278
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2173 5663 1937
Size: 236 3490 4594
Fragments arranged by size:
4594 3490 236
Acc65I G'GTAC_C
Cuts at: 5641 5641
Size: 8320
AclI AA'CG_TT
Cuts at: 1563 6447 6820 1563
Size: 4884 373 3063
Fragments arranged by size:
4884 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2720 3662 4403 4428 5044 5186 5195 6383
Size: 1088 942 741 25 616 142 9 1188
Cuts at: 6383 7767 8035 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1188 1088 942 741 616 268
142 25 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8123
AflIII A'CryG_T
Cuts at: 1203 7219 8196 1203
Size: 6016 977 1327
Fragments arranged by size:
6016 1327 977
AgeI A'CCGG_T
Cuts at: 7151 7151
Size: 8320
AhdI GACnn_n'nnGTC
Cuts at: 4508 7049 4508
Size: 2541 5779
AleI CACnn'nnGTG
Cuts at: 1166 4295 4904 1166
Size: 3129 609 4582
Fragments arranged by size:
4582 3129 609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3372 5585 1184
Size: 2188 2213 3919
Fragments arranged by size:
3919 2213 2188
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 8288
AlwNI CAG_nnn'CTG
Cuts at: 5092 6063 7635 5092
Size: 971 1572 5777
Fragments arranged by size:
5777 1572 971
ApaLI G'TGCA_C
Cuts at: 6377 7533 6377
Size: 1156 7164
ApoI r'AATT_y
Cuts at: 1493 4181 4823 5042 5408 5629 5855 6007 8116
Size: 2688 642 219 366 221 226 152 2109
Cuts at: 8116 1493
Size: 1697
Fragments arranged by size:
2688 2109 1697 642 366 226 221 219
152
AseI AT'TA_AT
Cuts at: 160 1032 2136 6874 160
Size: 872 1104 4738 1606
Fragments arranged by size:
4738 1606 1104 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 4113 5645 1870
Size: 73 1734 436 1532 4545
Fragments arranged by size:
4545 1734 1532 436 73
BamHI G'GATC_C
Cuts at: 5650 6017 5650
Size: 367 7953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3034 3779 4036 4710 4852
Size: 325 217 627 1247 745 257 674 142
Cuts at: 4852 5192 5641 7096 618
Size: 340 449 1455 1842
Fragments arranged by size:
1842 1455 1247 745 674 627 449 340
325 257 217 142
BanII G_rGCy'C
Cuts at: 1391 2717 3891 3954 4686 5639 1391
Size: 1326 1174 63 732 953 4072
Fragments arranged by size:
4072 1326 1174 953 732 63
BbeI G_GCGC'C
Cuts at: 3783 4714 3783
Size: 931 7389
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3481 928
Size: 971 1582 5767
Fragments arranged by size:
5767 1582 971
BbvCI CC'TCA_GC
Cuts at: 5075 5075
Size: 8320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 6511 6545 1903
Size: 34 1928 34 2612 34 3678
Fragments arranged by size:
3678 2612 1928 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5312 6212 7422 5312
Size: 900 1210 6210
Fragments arranged by size:
6210 1210 900
BclI T'GATC_A
Cuts at: 1548 2481 4644 1548
Size: 933 2163 5224
Fragments arranged by size:
5224 2163 933
BfrBI ATG'CAT
Cuts at: 4775 4775
Size: 8320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3274 4719 6931
Size: 107 122 71 631 605 1602 1445 2212
Cuts at: 6931 136
Size: 1525
Fragments arranged by size:
2212 1602 1525 1445 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2218 3637 4239 8315 2218
Size: 1419 602 4076 2223
Fragments arranged by size:
4076 2223 1419 602
BlpI GC'TnA_GC
Cuts at: 5737 5737
Size: 8320
Bme1580I G_kGCm'C
Cuts at: 1790 3678 5015 6381 7537 1790
Size: 1888 1337 1366 1156 2573
Fragments arranged by size:
2573 1888 1366 1337 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5575 6051 7009
Size: 416 53 83 399 24 4158 476 958
Cuts at: 7009 442
Size: 1753
Fragments arranged by size:
4158 1753 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2351 3549 4605 6980 1239
Size: 1004 108 1198 1056 2375 2579
Fragments arranged by size:
2579 2375 1198 1056 1004 108
Bpu10I CC'TnA_GC
Cuts at: 4248 5075 4248
Size: 827 7493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6445 7310 7608 6445
Size: 865 298 7157
Fragments arranged by size:
7157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3359 6983 882
Size: 617 1860 3624 2219
Fragments arranged by size:
3624 2219 1860 617
BsaAI yAC'GTr
Cuts at: 493 1204 6111 493
Size: 711 4907 2702
Fragments arranged by size:
4907 2702 711
BsaBI GATnn'nnATC
Cuts at: 5033 8314 5033
Size: 3281 5039
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3780 4711 6127 6509
Size: 53 83 186 936 2247 931 1416 382
Cuts at: 6509 7144 275
Size: 635 1451
Fragments arranged by size:
2247 1451 1416 936 931 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3822 3909 6753 7151 7425 7572 7983 3822
Size: 87 2844 398 274 147 411 4159
Fragments arranged by size:
4159 2844 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3171 3201 4508
Size: 30 165 30 1152 30 586 30 1307
Cuts at: 4508 4538 1178
Size: 30 4960
Fragments arranged by size:
4960 1307 1152 586 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3695 5219 5435 5438 5474 1186
Size: 2509 1524 216 3 36 4032
Fragments arranged by size:
4032 2509 1524 216 36 3
BseYI C'CCAG_C
Cuts at: 4871 5183 7523 8019 4871
Size: 312 2340 496 5172
Fragments arranged by size:
5172 2340 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2059 4643 5591 1163
Size: 573 323 2584 948 3892
Fragments arranged by size:
3892 2584 948 573 323
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5770 6531 7559 1540
Size: 275 186 3769 761 1028 2301
Fragments arranged by size:
3769 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2717 3619 3734 5505 6381 6466 7537 2717
Size: 902 115 1771 876 85 1071 3500
Fragments arranged by size:
3500 1771 1071 902 876 115 85
BsmI GAATG_Cn'
Cuts at: 4137 4775 5355 5831 5924 4137
Size: 638 580 476 93 6533
Fragments arranged by size:
6533 638 580 476 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2717 3619 3678 3734 3891 3954 4686
Size: 399 927 902 59 56 157 63 732
Cuts at: 4686 5015 5505 5639 6381 6466 7537 1391
Size: 329 490 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 927 902 742 732 490 399
329 157 134 85 63 59 56
BspEI T'CCGG_A
Cuts at: 3909 7983 3909
Size: 4074 4246
BspHI T'CATG_A
Cuts at: 5394 6209 7939 5394
Size: 815 1730 5775
Fragments arranged by size:
5775 1730 815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2227 3583 4327 5675 844
Size: 1383 1356 744 1348 3489
Fragments arranged by size:
3489 1383 1356 1348 744
BsrBI CCG'CTC
Cuts at: 1394 1823 6207 1394
Size: 429 4384 3507
Fragments arranged by size:
4384 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4119 6815 6989 65
Size: 1183 2871 2696 174 1396
Fragments arranged by size:
2871 2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3101 4188 4332 6964 7151 1700
Size: 110 1291 1087 144 2632 187 2869
Fragments arranged by size:
2869 2632 1291 1087 187 144 110
BsrGI T'GTAC_A
Cuts at: 96 2155 3699 3768 5066 96
Size: 2059 1544 69 1298 3350
Fragments arranged by size:
3350 2059 1544 1298 69
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8320
BssSI C'ACGA_G
Cuts at: 6380 7392 6380
Size: 1012 7308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8320
BstBI TT'CG_AA
Cuts at: 7124 7124
Size: 8320
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8320
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 3301 3505 2044
Size: 1257 204 6859
Fragments arranged by size:
6859 1257 204
BstYI r'GATC_y
Cuts at: 1972 2218 3637 3975 4239 4944 5274 5650 6017
Size: 246 1419 338 264 705 330 376 367
Cuts at: 6017 6402 6419 7871 7957 7969 8315 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1419 705 385 376 367 346
338 330 264 246 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3182 3369 3891 4407 4948 513
Size: 552 2117 187 522 516 541 3885
Fragments arranged by size:
3885 2117 552 541 522 516 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4399 487
Size: 683 56 3173 4408
Fragments arranged by size:
4408 3173 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3843 4497 5836 6630 6650 2552
Size: 1291 654 1339 794 20 4222
Fragments arranged by size:
4222 1339 1291 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8285
DraI TTT'AAA
Cuts at: 5624 5624
Size: 8320
DraIII CAC_nnn'GTG
Cuts at: 2254 2254
Size: 8320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3515 7327 809
Size: 507 2199 3812 1802
Fragments arranged by size:
3812 2199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2803 4563
Size: 54 1425 50 275 18 216 757 1760
Cuts at: 4563 4674 4720 5767 6656 8285 8
Size: 111 46 1047 889 1629 43
Fragments arranged by size:
1760 1629 1425 1047 889 757 275 216
111 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5767 1537
Size: 275 3955 4090
Fragments arranged by size:
4090 3955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 4925 5108 5451 6250 1353
Size: 957 2615 183 343 799 3423
Fragments arranged by size:
3423 2615 957 799 343 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6907 7281 7427 387
Size: 1064 138 5318 374 146 1280
Fragments arranged by size:
5318 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2715 2715
Size: 8320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2351 2720 3549 3662 4403 4428
Size: 393 611 108 369 829 113 741 25
Cuts at: 4428 4605 5044 5186 5195 6383 6980 7767 8035
Size: 177 439 142 9 1188 597 787 268
Cuts at: 8035 1239
Size: 1524
Fragments arranged by size:
1524 1188 829 787 741 611 597 439
393 369 268 177 142 113 108 25
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2193 5177 5450 2193
Size: 2984 273 5063
Fragments arranged by size:
5063 2984 273
EcoO109I rG'GnC_Cy
Cuts at: 2781 4018 4258 5290 2781
Size: 1237 240 1032 5811
Fragments arranged by size:
5811 1237 1032 240
EcoRI G'AATT_C
Cuts at: 4181 5408 5629 6007 4181
Size: 1227 221 378 6494
Fragments arranged by size:
6494 1227 378 221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 4780 4981 2299
Size: 2481 201 5638
Fragments arranged by size:
5638 2481 201
FspI TGC'GCA
Cuts at: 3719 3800 6826 3719
Size: 81 3026 5213
Fragments arranged by size:
5213 3026 81
HaeII r_GCGC'y
Cuts at: 1678 3783 4714 7467 1678
Size: 2105 931 2753 2531
Fragments arranged by size:
2753 2531 2105 931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 3171 3203 3547
Size: 32 135 32 1127 32 440 32 344
Cuts at: 3547 3579 4494 4508 4526 4540 4578 4610 5259
Size: 32 915 14 18 14 38 32 649
Cuts at: 5259 5291 6961 6993 7035 7067 1373
Size: 32 1670 32 42 32 2626
Fragments arranged by size:
2626 1670 1127 915 649 440 344 135
42 38 32 32 32 32 32 32
32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2594 2833 3414 5664 5910 669
Size: 988 281 656 239 581 2250 246 3079
Fragments arranged by size:
3079 2250 988 656 581 281 246 239
HindIII A'AGCT_T
Cuts at: 748 3441 4250 748
Size: 2693 809 4818
Fragments arranged by size:
4818 2693 809
HpaI GTT'AAC
Cuts at: 5910 5910
Size: 8320
KasI G'GCGC_C
Cuts at: 3779 4710 3779
Size: 931 7389
KpnI G_GTAC'C
Cuts at: 5645 5645
Size: 8320
MfeI C'AATT_G
Cuts at: 5919 5919
Size: 8320
MluI A'CGCG_T
Cuts at: 8196 8196
Size: 8320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5490 5565 7434 7618 1305
Size: 4185 75 1869 184 2007
Fragments arranged by size:
4185 2007 1869 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4676 10
Size: 54 1425 343 2844 3654
Fragments arranged by size:
3654 2844 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2489 3354 3431 4295 4751 4904
Size: 648 915 408 865 77 864 456 153
Cuts at: 4904 6278 6637 6796 518
Size: 1374 359 159 2042
Fragments arranged by size:
2042 1374 915 865 864 648 456 408
359 159 153 77
MspA1I CmG'CkG
Cuts at: 1606 2822 5736 6413 7561 7806 1606
Size: 1216 2914 677 1148 245 2120
Fragments arranged by size:
2914 2120 1216 1148 677 245
NarI GG'CG_CC
Cuts at: 3780 4711 3780
Size: 931 7389
NcoI C'CATG_G
Cuts at: 513 1065 3182 3369 3891 4407 4948 513
Size: 552 2117 187 522 516 541 3885
Fragments arranged by size:
3885 2117 552 541 522 516 187
NdeI CA'TA_TG
Cuts at: 387 5530 387
Size: 5143 3177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8320
NotI GC'GGCC_GC
Cuts at: 5767 5767
Size: 8320
NsiI A_TGCA'T
Cuts at: 4777 4777
Size: 8320
NspI r_CATG'y
Cuts at: 1132 1229 5678 7223 1132
Size: 97 4449 1545 2229
Fragments arranged by size:
4449 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8320
PciI A'CATG_T
Cuts at: 7219 7219
Size: 8320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 6637
PfoI T'CCnGG_A
Cuts at: 2328 3504 3816 5445 2328
Size: 1176 312 1629 5203
Fragments arranged by size:
5203 1629 1176 312
PmeI GTTT'AAAC
Cuts at: 5624 5624
Size: 8320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 6389 6421 7902 7934 2555
Size: 32 3802 32 1481 32 2941
Fragments arranged by size:
3802 2941 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2781 4018 4258 2781
Size: 1237 240 6843
Fragments arranged by size:
6843 1237 240
PshAI GACnn'nnGTC
Cuts at: 1613 2786 1613
Size: 1173 7147
PsiI TTA'TAA
Cuts at: 5890 5890
Size: 8320
PstI C_TGCA'G
Cuts at: 830 2171 2218 2547 2671 3063 4539 5672 830
Size: 1341 47 329 124 392 1476 1133 3478
Fragments arranged by size:
3478 1476 1341 1133 392 329 124 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8320
PvuII CAG'CTG
Cuts at: 2822 2822
Size: 8320
SacI G_AGCT'C
Cuts at: 2717 2717
Size: 8320
SalI G'TCGA_C
Cuts at: 1936 5662 1936
Size: 3726 4594
SanDI GG'GwC_CC
Cuts at: 2781 2781
Size: 8320
SapI GCTCTTCn'nnn_
Cuts at: 2310 4925 2310
Size: 2615 5705
SbfI CC_TGCA'GG
Cuts at: 5672 5672
Size: 8320
ScaI AGT'ACT
Cuts at: 3218 6568 8280 3218
Size: 3350 1712 3258
Fragments arranged by size:
3350 3258 1712
SfcI C'TryA_G
Cuts at: 826 1045 2167 2214 2543 2667 3059 4535 5001
Size: 219 1122 47 329 124 392 1476 466
Cuts at: 5001 5597 5668 6803 7484 7675 826
Size: 596 71 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 1122 681 596 466 392
329 219 191 124 71 47
SfoI GGC'GCC
Cuts at: 3781 4712 3781
Size: 931 7389
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8320
SmaI CCC'GGG
Cuts at: 1872 4115 5647 1872
Size: 2243 1532 4545
Fragments arranged by size:
4545 2243 1532
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6424 7325 7587 820
Size: 197 926 4481 901 262 1553
Fragments arranged by size:
4481 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6111 493
Size: 5618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8320
SphI G_CATG'C
Cuts at: 1132 5678 1132
Size: 4546 3774
SrfI GCCC'GGGC
Cuts at: 4115 4115
Size: 8320
SspI AAT'ATT
Cuts at: 5 52 6244 5
Size: 47 6192 2081
Fragments arranged by size:
6192 2081 47
StuI AGG'CCT
Cuts at: 1517 3582 1517
Size: 2065 6255
StyI C'CwwG_G
Cuts at: 513 1065 2686 3182 3369 3891 4407 4948 5214
Size: 552 1621 496 187 522 516 541 266
Cuts at: 5214 5759 513
Size: 545 3074
Fragments arranged by size:
3074 1621 552 545 541 522 516 496
266 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 4885 6347 6364 6517 6702 7147
Size: 1255 2043 1262 1462 17 153 185 445
Cuts at: 7147 325
Size: 1498
Fragments arranged by size:
2043 1498 1462 1262 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 3197 3216
Size: 274 80 33 51 1443 178 1042 19
Cuts at: 3216 3250 3699 3768 5066 6566 8278 96
Size: 34 449 69 1298 1500 1712 138
Fragments arranged by size:
1712 1500 1443 1298 1042 449 274 178
138 80 69 51 34 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 8280 8312 2238
Size: 32 6010 32 2246
Fragments arranged by size:
6010 2246 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 2738 1316
Size: 708 714 6898
Fragments arranged by size:
6898 714 708
XbaI T'CTAG_A
Cuts at: 5656 5656
Size: 8320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 8320
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8320
XmaI C'CCGG_G
Cuts at: 1870 4113 5645 1870
Size: 2243 1532 4545
Fragments arranged by size:
4545 2243 1532
XmnI GAAnn'nnTTC
Cuts at: 2304 2531 5355 6449 2304
Size: 227 2824 1094 4175
Fragments arranged by size:
4175 2824 1094 227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6128 276
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SwaI