Restriction map for
pFN21AB5567
(Circular) (Six-base) MAPSORT of: pFN21AB5567.seq Check: 8179 from: 1 to: 6721
pFN21AB5567 6721 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3045 3194 4784 6168 6436 1632
Size: 1413 149 1590 1384 268 1917
Fragments arranged by size:
1917 1590 1413 1384 268 149
AfeI AGC'GCT
Cuts at: 3623 3623
Size: 6721
AflII C'TTAA_G
Cuts at: 820 1017 3133 820
Size: 197 2116 4408
Fragments arranged by size:
4408 2116 197
AflIII A'CryG_T
Cuts at: 1203 2134 5620 6597 1203
Size: 931 3486 977 1327
Fragments arranged by size:
3486 1327 977 931
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 3038 3602 4464 6036 3038
Size: 564 862 1572 3723
Fragments arranged by size:
3723 1572 862 564
ApaI G_GGCC'C
Cuts at: 3518 3518
Size: 6721
ApaLI G'TGCA_C
Cuts at: 2931 4778 5934 2931
Size: 1847 1156 3718
Fragments arranged by size:
3718 1847 1156
ApoI r'AATT_y
Cuts at: 1493 3141 3469 3656 3983 4030 4256 4408 6517
Size: 1648 328 187 327 47 226 152 2109
Cuts at: 6517 1493
Size: 1697
Fragments arranged by size:
2109 1697 1648 328 327 226 187 152
47
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 4046 1870
Size: 73 2103 4545
Fragments arranged by size:
4545 2103 73
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2710 4042 5497 618
Size: 325 217 627 923 1332 1455 1842
Fragments arranged by size:
1842 1455 1332 923 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2472 3518 3558 4040 1391
Size: 1081 1046 40 482 4072
Fragments arranged by size:
4072 1081 1046 482 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2843 3051 3247 3435 928
Size: 971 944 208 196 188 4214
Fragments arranged by size:
4214 971 944 208 196 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5823 4613
Size: 1210 5511
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5332 136
Size: 107 122 71 631 605 3660 1525
Fragments arranged by size:
3660 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2009 6716 2009
Size: 4707 2014
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 2713 2935 3518 4782 5938 1790
Size: 923 222 583 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 923 583 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3245 4452 5410
Size: 416 53 83 399 24 1828 1207 958
Cuts at: 5410 442
Size: 1753
Fragments arranged by size:
1828 1753 1207 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3002 3472 3882 5381 1239
Size: 1763 470 410 1499 2579
Fragments arranged by size:
2579 1763 1499 470 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2815 3436 3563 4846 5711 6009 2815
Size: 621 127 1283 865 298 3527
Fragments arranged by size:
3527 1283 865 621 298 127
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 2865 3581 5384 882
Size: 617 843 523 716 1803 2219
Fragments arranged by size:
2219 1803 843 716 617 523
BsaAI yAC'GTr
Cuts at: 493 1204 3331 4512 493
Size: 711 2127 1181 2702
Fragments arranged by size:
2702 2127 1181 711
BsaBI GATnn'nnATC
Cuts at: 2650 6715 2650
Size: 4065 2656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2603 4528 4910 5545
Size: 53 83 186 936 1070 1925 382 635
Cuts at: 5545 275
Size: 1451
Fragments arranged by size:
1925 1451 1070 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2077 2563 5154 5552 5826 5973 6384 2077
Size: 486 2591 398 274 147 411 2414
Fragments arranged by size:
2591 2414 486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2529 2559 3710 3740 3849
Size: 30 165 30 1126 30 1151 30 109
Cuts at: 3849 3865 3879 3895 1178
Size: 16 14 16 4004
Fragments arranged by size:
4004 1151 1126 165 109 30 30 30
30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6721
BseYI C'CCAG_C
Cuts at: 2783 3053 5924 6420 2783
Size: 270 2871 496 3084
Fragments arranged by size:
3084 2871 496 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3720 1163
Size: 573 1984 4164
Fragments arranged by size:
4164 1984 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4171 4932 5960 1540
Size: 275 186 2170 761 1028 2301
Fragments arranged by size:
2301 2170 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2128 2935 3558 4782 4867 5938 2128
Size: 807 623 1224 85 1071 2911
Fragments arranged by size:
2911 1224 1071 807 623 85
BsmI GAATG_Cn'
Cuts at: 2673 3178 4232 4325 2673
Size: 505 1054 93 5069
Fragments arranged by size:
5069 1054 505 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2610 2791 2610
Size: 181 6540
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2472 2713 2935 3518 3558 4040
Size: 399 338 344 241 222 583 40 482
Cuts at: 4040 4782 4867 5938 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 482 399 344 338
241 222 85 40
BspEI T'CCGG_A
Cuts at: 2077 2563 6384 2077
Size: 486 3821 2414
Fragments arranged by size:
3821 2414 486
BspHI T'CATG_A
Cuts at: 2854 4610 6340 2854
Size: 1756 1730 3235
Fragments arranged by size:
3235 1756 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3659 4076 844
Size: 2815 417 3489
Fragments arranged by size:
3489 2815 417
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3360 5216 5390 65
Size: 1183 2112 1856 174 1396
Fragments arranged by size:
2112 1856 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2939 794
Size: 2145 4576
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 3961 3961
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2009 2557 4051 4418 4803 4820 6272 6358
Size: 37 548 1494 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1494 1452 548 385 367 346 86
37 17 12
Bsu36I CC'TnA_GG
Cuts at: 3298 3774 3298
Size: 476 6245
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 4237 5031 5051 4237
Size: 794 20 5907
Fragments arranged by size:
5907 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 4025 4025
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5728 809
Size: 507 4412 1802
Fragments arranged by size:
4412 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2726 4168 5057
Size: 54 1425 50 275 18 896 1442 889
Cuts at: 5057 6686 8
Size: 1629 43
Fragments arranged by size:
1629 1442 1425 896 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2613 3565 3832 4651 1353
Size: 1047 213 952 267 819 3423
Fragments arranged by size:
3423 1047 952 819 267 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5308 5682 5828 387
Size: 1064 138 3719 374 146 1280
Fragments arranged by size:
3719 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3556 3556
Size: 6721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3002 3045 3194 3472 3882 4784 5381
Size: 393 1370 43 149 278 410 902 597
Cuts at: 5381 6168 6436 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1370 902 787 597 410 393 278
268 149 43
EcoO109I rG'GnC_Cy
Cuts at: 2843 3514 3515 2843
Size: 671 1 6049
Fragments arranged by size:
6049 671 1
EcoRI G'AATT_C
Cuts at: 4030 4408 4030
Size: 378 6343
EcoRV GAT'ATC
Cuts at: 3362 3362
Size: 6721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2393 3876 2393
Size: 1483 5238
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 2784 3625 5868 1678
Size: 1106 841 2243 2531
Fragments arranged by size:
2531 2243 1106 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3175 3207 3395
Size: 32 135 32 970 32 601 32 188
Cuts at: 3395 3427 3757 3789 3865 3897 5362 5394 5436
Size: 32 330 32 76 32 1465 32 42
Cuts at: 5436 5468 1373
Size: 32 2626
Fragments arranged by size:
2626 1465 970 601 330 188 135 76
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4065 4311 669
Size: 988 281 2127 246 3079
Fragments arranged by size:
3079 2127 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6721
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 4320 4320
Size: 6721
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2222 3389 4013 5835 6019 1305
Size: 825 92 1167 624 1822 184 2007
Fragments arranged by size:
2007 1822 1167 825 624 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2728 10
Size: 54 1425 343 896 4003
Fragments arranged by size:
4003 1425 896 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4679 5038 5197 518
Size: 648 3513 359 159 2042
Fragments arranged by size:
3513 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3800 3878 3932 4137 4814 5962 6207 1606
Size: 2194 78 54 205 677 1148 245 2120
Fragments arranged by size:
2194 2120 1148 677 245 205 78 54
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6169
NdeI CA'TA_TG
Cuts at: 387 3289 3916 387
Size: 2902 627 3192
Fragments arranged by size:
3192 2902 627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 2138 2618 2942 4079 5624 1132
Size: 97 909 480 324 1137 1545 2229
Fragments arranged by size:
2229 1545 1137 909 480 324 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 2134 5620 2134
Size: 3486 3235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 3905 1108
Size: 939 1858 3924
Fragments arranged by size:
3924 1858 939
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2843 2843
Size: 6721
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6721
PsiI TTA'TAA
Cuts at: 2450 4291 2450
Size: 1841 4880
PspOMI G'GGCC_C
Cuts at: 3514 3514
Size: 6721
PstI C_TGCA'G
Cuts at: 830 3811 4073 830
Size: 2981 262 3478
Fragments arranged by size:
3478 2981 262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 3800 3878 3932 3800
Size: 78 54 6589
Fragments arranged by size:
6589 78 54
SacI G_AGCT'C
Cuts at: 3558 3558
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 4969 6681 4969
Size: 1712 5009
SfcI C'TryA_G
Cuts at: 826 1045 2754 3267 3807 4069 5204 5885 6076
Size: 219 1709 513 540 262 1135 681 191
Cuts at: 6076 826
Size: 1471
Fragments arranged by size:
1709 1471 1135 681 540 513 262 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 4048 1872
Size: 2176 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2794 3133 3451 3578 4825 5726
Size: 197 926 851 339 318 127 1247 901
Cuts at: 5726 5988 820
Size: 262 1553
Fragments arranged by size:
1553 1247 926 901 851 339 318 262
197 127
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 2942 4079 1132
Size: 1810 1137 3774
Fragments arranged by size:
3774 1810 1137
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 2792 3202 1517
Size: 1275 410 5036
Fragments arranged by size:
5036 1275 410
StyI C'CwwG_G
Cuts at: 513 1065 2226 2598 3714 4160 513
Size: 552 1161 372 1116 446 3074
Fragments arranged by size:
3074 1161 1116 552 446 372
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4748 4765 4918 5103 5548 325
Size: 1255 3168 17 153 185 445 1498
Fragments arranged by size:
3168 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4967 6679 96
Size: 274 80 33 51 1443 2990 1712 138
Fragments arranged by size:
2990 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 6681 6713 2236
Size: 32 4413 32 2244
Fragments arranged by size:
4413 2244 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2721 2721
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 4046 1870
Size: 2176 4545
XmnI GAAnn'nnTTC
Cuts at: 2094 4850 2094
Size: 2756 3965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI