Restriction map for
pFN21AB5566
(Circular) (Six-base) MAPSORT of: pFN21AB5566.seq Check: 449 from: 1 to: 5497
pFN21AB5566 5497 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 2840 1937
Size: 813 90 4594
Fragments arranged by size:
4594 813 90
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3560 4944 5212 1632
Size: 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5300
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 2694 4328 2694
Size: 1634 3863
AhdI GACnn_n'nnGTC
Cuts at: 4226 4226
Size: 5497
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2678 1184
Size: 1494 4003
AlwNI CAG_nnn'CTG
Cuts at: 2584 3240 4812 2584
Size: 656 1572 3269
Fragments arranged by size:
3269 1572 656
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2822 1870
Size: 73 879 4545
Fragments arranged by size:
4545 879 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2266 2299 2266
Size: 33 5464
BamHI G'GATC_C
Cuts at: 2101 2222 2827 3194 2101
Size: 121 605 367 4404
Fragments arranged by size:
4404 605 367 121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2818 4273 618
Size: 325 217 627 507 524 1455 1842
Fragments arranged by size:
1842 1455 627 524 507 325 217
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 2781 1548
Size: 1233 4264
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2577 2618 2914 2577
Size: 41 296 5160
Fragments arranged by size:
5160 296 41
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4714 1790
Size: 1768 1156 2573
Fragments arranged by size:
2573 1768 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2773 3228 4186
Size: 416 53 83 399 24 1356 455 958
Cuts at: 4186 442
Size: 1753
Fragments arranged by size:
1753 1356 958 455 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 4157 1239
Size: 1382 1536 2579
Fragments arranged by size:
2579 1536 1382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 4487 4785 3622
Size: 865 298 4334
Fragments arranged by size:
4334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2750 4160 882
Size: 617 1251 1410 2219
Fragments arranged by size:
2219 1410 1251 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 5491 5491
Size: 5497
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 3304 3686 4321
Size: 53 83 186 936 575 1196 382 635
Cuts at: 4321 275
Size: 1451
Fragments arranged by size:
1451 1196 936 635 575 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2030 2694 3930 4328 4602 4749 5160 2030
Size: 664 1236 398 274 147 411 2367
Fragments arranged by size:
2367 1236 664 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5272
Fragments arranged by size:
5272 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 1186
Size: 1189 4308
BseYI C'CCAG_C
Cuts at: 2218 2290 4700 5196 2218
Size: 72 2410 496 2519
Fragments arranged by size:
2519 2410 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2097 1163
Size: 573 361 4563
Fragments arranged by size:
4563 573 361
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3558 3643 4714 3558
Size: 85 1071 4341
Fragments arranged by size:
4341 1071 85
BsmI GAATG_Cn'
Cuts at: 2073 3008 3101 2073
Size: 935 93 4469
Fragments arranged by size:
4469 935 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 3558 3643 4714 1391
Size: 399 1026 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 399 85
BspEI T'CCGG_A
Cuts at: 2030 5160 2030
Size: 3130 2367
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2852 844
Size: 2008 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2433 2694 4141 4328 1700
Size: 110 623 261 1447 187 2869
Fragments arranged by size:
2869 1447 623 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 96
Size: 2331 3166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 3677
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 4526
BstYI r'GATC_y
Cuts at: 1972 2101 2222 2827 3194 3579 3596 5048 5134
Size: 129 121 605 367 385 17 1452 86
Cuts at: 5134 5146 5492 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 605 385 367 346 129 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2466 513
Size: 552 939 147 315 3544
Fragments arranged by size:
3544 939 552 315 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2341 2550 2944
Size: 54 1425 50 275 18 511 209 394
Cuts at: 2944 3833 5462 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 511 394 275 209 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2208 4084 4458 4604 387
Size: 1064 138 619 1876 374 146 1280
Fragments arranged by size:
1876 1280 1064 619 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2621 3560 4157 4944 5212 1239
Size: 393 989 939 597 787 268 1524
Fragments arranged by size:
1524 989 939 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2163 2163
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2440 4644 1678
Size: 762 2204 2531
Fragments arranged by size:
2531 2204 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2115 2125 2147 4138
Size: 32 135 32 521 22 10 22 1991
Cuts at: 4138 4170 4212 4244 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1991 521 135 42 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2841 3087 669
Size: 988 281 337 566 246 3079
Fragments arranged by size:
3079 988 566 337 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2552 10
Size: 54 1425 343 720 2955
Fragments arranged by size:
2955 1425 720 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 3455 3814 3973 518
Size: 648 1494 795 359 159 2042
Fragments arranged by size:
2042 1494 795 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NaeI GCC'GGC
Cuts at: 2435 2435
Size: 5497
NcoI C'CATG_G
Cuts at: 513 1065 2004 2151 2466 513
Size: 552 939 147 315 3544
Fragments arranged by size:
3544 939 552 315 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NgoMIV G'CCGG_C
Cuts at: 2433 2433
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2218 2521 2659 2855 4400 1132
Size: 97 989 303 138 196 1545 2229
Fragments arranged by size:
2229 1545 989 303 196 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5497
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5497
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 5079 5111 3566
Size: 32 1481 32 3952
Fragments arranged by size:
3952 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 2163
Size: 5497
PshAI GACnn'nnGTC
Cuts at: 1613 2168 2746 1613
Size: 555 578 4364
Fragments arranged by size:
4364 578 555
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2849 830
Size: 2019 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2849 2849
Size: 5497
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2386 2751 2845 3980 4661 4852 826
Size: 219 1341 365 94 1135 681 191 1471
Fragments arranged by size:
1471 1341 1135 681 365 219 191 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3601 4502 4764 820
Size: 197 926 1658 901 262 1553
Fragments arranged by size:
1658 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2659 2855 1132
Size: 1527 196 3774
Fragments arranged by size:
3774 1527 196
SspI AAT'ATT
Cuts at: 5 52 3421 5
Size: 47 3369 2081
Fragments arranged by size:
3369 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2235 2466 2936 513
Size: 552 939 147 84 231 470 3074
Fragments arranged by size:
3074 939 552 470 231 147 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2642 3524 3541 3694 3879 4324
Size: 1255 702 360 882 17 153 185 445
Cuts at: 4324 325
Size: 1498
Fragments arranged by size:
1498 1255 882 702 445 360 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 2667 3743
Size: 274 80 33 51 1443 450 240 1076
Cuts at: 3743 5455 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 450 274 240 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2783 2783
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI