Restriction map for
pFN21AB5561
(Circular) (Six-base) MAPSORT of: pFN21AB5561.seq Check: 3844 from: 1 to: 6196
pFN21AB5561 6196 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3352 3457 3539 1937
Size: 1415 105 82 4594
Fragments arranged by size:
4594 1415 105 82
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 3036 3102 4259 5643 5911 1632
Size: 800 604 66 1157 1384 268 1917
Fragments arranged by size:
1917 1384 1157 800 604 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 2037 3410 5095 6072 1203
Size: 834 1373 1685 977 1327
Fragments arranged by size:
1685 1373 1327 977 834
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 3939 5511 3939
Size: 1572 4624
ApaI G_GGCC'C
Cuts at: 2995 2995
Size: 6196
ApaLI G'TGCA_C
Cuts at: 2148 4253 5409 2148
Size: 2105 1156 2935
Fragments arranged by size:
2935 2105 1156
ApoI r'AATT_y
Cuts at: 1493 2533 2765 2910 3505 3731 3883 5992 1493
Size: 1040 232 145 595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 595 232 226 152 145
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 3521 1870
Size: 73 1578 4545
Fragments arranged by size:
4545 1578 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2370 2403 2370
Size: 33 6163
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3517 4972 618
Size: 325 217 627 1730 1455 1842
Fragments arranged by size:
1842 1730 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2995 3515 1391
Size: 1604 520 4072
Fragments arranged by size:
4072 1604 520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 928
Size: 971 1305 3920
Fragments arranged by size:
3920 1305 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 2422 1548
Size: 874 5322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2995 4257 5413 1790
Size: 362 843 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 843 362
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3443 3927 4885
Size: 416 53 83 399 24 2026 484 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
2026 1753 958 484 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4856 1239
Size: 3617 2579
Bpu10I CC'TnA_GC
Cuts at: 2917 2917
Size: 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4321 5186 5484 4321
Size: 865 298 5033
Fragments arranged by size:
5033 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 4859 882
Size: 617 591 2769 2219
Fragments arranged by size:
2769 2219 617 591
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2675 4003 4385 5020
Size: 53 83 186 936 1142 1328 382 635
Cuts at: 5020 275
Size: 1451
Fragments arranged by size:
1451 1328 1142 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2686 4629 5027 5301 5448 5859 2686
Size: 1943 398 274 147 411 3023
Fragments arranged by size:
3023 1943 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5971
Fragments arranged by size:
5971 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 1186
Size: 840 5356
BseYI C'CCAG_C
Cuts at: 5399 5895 5399
Size: 496 5700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5623
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 2310 4257 4342 5413 2152
Size: 158 1947 85 1071 2935
Fragments arranged by size:
2935 1947 1071 158 85
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2310 2995 3515 4257 4342 5413
Size: 399 362 158 685 520 742 85 1071
Cuts at: 5413 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 685 520 399 362 158
85
BspEI T'CCGG_A
Cuts at: 2686 5859 2686
Size: 3173 3023
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3551 844
Size: 2707 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 2953 2974 4691 4865 65
Size: 1183 869 836 21 1717 174 1396
Fragments arranged by size:
1717 1396 1183 869 836 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 4840 5027 1700
Size: 110 3030 187 2869
Fragments arranged by size:
3030 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2437 96
Size: 2341 3855
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2943 794
Size: 2149 4047
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2689 2902 3526 3893 4278 4295 5747 5833
Size: 717 213 624 367 385 17 1452 86
Cuts at: 5833 5845 6191 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 717 624 385 367 346 213
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3376 513
Size: 552 939 1372 3333
Fragments arranged by size:
3333 1372 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 3712 4506 4526 2665
Size: 1047 794 20 4335
Fragments arranged by size:
4335 1047 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 3643 4532
Size: 54 1425 50 275 18 364 1449 889
Cuts at: 4532 6161 8
Size: 1629 43
Fragments arranged by size:
1629 1449 1425 889 364 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 3078 3285 4126 1353
Size: 1095 630 207 841 3423
Fragments arranged by size:
3423 1095 841 630 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 3036 3102 4259 4856 5643 5911
Size: 393 800 604 66 1157 597 787 268
Cuts at: 5911 1239
Size: 1524
Fragments arranged by size:
1524 1157 800 787 604 597 393 268
66
EcoO109I rG'GnC_Cy
Cuts at: 2431 2992 2431
Size: 561 5635
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 2198
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 5343 1678
Size: 3665 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2531 2563 2808 2840 4837
Size: 32 135 32 959 32 245 32 1997
Cuts at: 4837 4869 4911 4943 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1997 959 245 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3353 3540 3786 669
Size: 988 281 1415 187 246 3079
Fragments arranged by size:
3079 1415 988 281 246 187
HindIII A'AGCT_T
Cuts at: 748 2290 3174 748
Size: 1542 884 3770
Fragments arranged by size:
3770 1542 884
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2709 5310 5494 1305
Size: 1404 2601 184 2007
Fragments arranged by size:
2601 2007 1404 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2196 10
Size: 54 1425 343 364 4010
Fragments arranged by size:
4010 1425 364 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2718 4154 4513 4672 518
Size: 648 1552 1436 359 159 2042
Fragments arranged by size:
2042 1552 1436 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3612 4289 5437 5682 1606
Size: 2006 677 1148 245 2120
Fragments arranged by size:
2120 2006 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3376 513
Size: 552 939 1372 3333
Fragments arranged by size:
3333 1372 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 2041 3414 3554 5099 1132
Size: 97 812 1373 140 1545 2229
Fragments arranged by size:
2229 1545 1373 812 140 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6196
PciI A'CATG_T
Cuts at: 2037 3410 5095 2037
Size: 1373 1685 3138
Fragments arranged by size:
3138 1685 1373
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 6196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PspOMI G'GGCC_C
Cuts at: 2991 2991
Size: 6196
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3351 3538 1936
Size: 1415 187 4594
Fragments arranged by size:
4594 1415 187
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2332 2667 3117 3544 4679 5360 5551
Size: 219 1287 335 450 427 1135 681 191
Cuts at: 5551 826
Size: 1471
Fragments arranged by size:
1471 1287 1135 681 450 427 335 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4300 5201 5463 820
Size: 197 926 2357 901 262 1553
Fragments arranged by size:
2357 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2004 3376 3635 513
Size: 552 939 1372 259 3074
Fragments arranged by size:
3074 1372 939 552 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4223 4240 4393 4578 5023 325
Size: 1255 2643 17 153 185 445 1498
Fragments arranged by size:
2643 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2437 4442 6154
Size: 274 80 33 51 1443 460 2005 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
2005 1712 1443 460 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 2483 2501 3532 2483
Size: 18 1031 5147
Fragments arranged by size:
5147 1031 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI