Restriction map for
pFN21AB5558
(Circular) (Six-base) MAPSORT of: pFN21AB5558.seq Check: 5689 from: 1 to: 6424
pFN21AB5558 6424 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3443 3443
Size: 6424
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4234 278
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2136 3767 1937
Size: 199 1631 4594
Fragments arranged by size:
4594 1631 199
Acc65I G'GTAC_C
Cuts at: 3745 3745
Size: 6424
AclI AA'CG_TT
Cuts at: 1563 4551 4924 1563
Size: 2988 373 3063
Fragments arranged by size:
3063 2988 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2520 2850 2873 3236 4487 5871 6139 1632
Size: 888 330 23 363 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1251 888 363 330 268 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6227
AflIII A'CryG_T
Cuts at: 1203 5323 6300 1203
Size: 4120 977 1327
Fragments arranged by size:
4120 1327 977
AgeI A'CCGG_T
Cuts at: 2041 5255 2041
Size: 3214 3210
AhdI GACnn_n'nnGTC
Cuts at: 5153 5153
Size: 6424
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6424
AlwNI CAG_nnn'CTG
Cuts at: 4167 5739 4167
Size: 1572 4852
ApaLI G'TGCA_C
Cuts at: 4481 5637 4481
Size: 1156 5268
ApoI r'AATT_y
Cuts at: 1493 2096 2717 2754 3423 3648 3733 3959 4111
Size: 603 621 37 669 225 85 226 152
Cuts at: 4111 6220 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 669 621 603 226 225 152
85 37
AseI AT'TA_AT
Cuts at: 160 1032 2691 4978 160
Size: 872 1659 2287 1606
Fragments arranged by size:
2287 1659 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2112 3749 1870
Size: 73 130 39 1637 4545
Fragments arranged by size:
4545 1637 130 73 39
BamHI G'GATC_C
Cuts at: 3754 4121 3754
Size: 367 6057
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3745 5200 618
Size: 325 217 627 1958 1455 1842
Fragments arranged by size:
1958 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3743 1391
Size: 2352 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2879 3015 928
Size: 971 980 136 4337
Fragments arranged by size:
4337 980 971 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4615 4649 1903
Size: 34 50 34 2594 34 3678
Fragments arranged by size:
3678 2594 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2774 4316 5526 2774
Size: 1542 1210 3672
Fragments arranged by size:
3672 1542 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6424
BfrBI ATG'CAT
Cuts at: 3345 3345
Size: 6424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5035 136
Size: 107 122 71 631 605 3363 1525
Fragments arranged by size:
3363 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6419 6419
Size: 6424
BlpI GC'TnA_GC
Cuts at: 3841 3841
Size: 6424
Bme1580I G_kGCm'C
Cuts at: 1790 3334 4485 5641 1790
Size: 1544 1151 1156 2573
Fragments arranged by size:
2573 1544 1156 1151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6424
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4155 5113 442
Size: 416 53 83 399 24 2738 958 1753
Fragments arranged by size:
2738 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2895 3354 3546 5084 1239
Size: 1656 459 192 1538 2579
Fragments arranged by size:
2579 1656 1538 459 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4549 5414 5712 4549
Size: 865 298 5261
Fragments arranged by size:
5261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2032 5087 882
Size: 617 533 3055 2219
Fragments arranged by size:
3055 2219 617 533
BsaAI yAC'GTr
Cuts at: 493 1204 4215 493
Size: 711 3011 2702
Fragments arranged by size:
3011 2702 711
BsaBI GATnn'nnATC
Cuts at: 6418 6418
Size: 6424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4231 4613 5248 275
Size: 53 83 186 936 2698 382 635 1451
Fragments arranged by size:
2698 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2041 4857 5255 5529 5676 6087 2041
Size: 2816 398 274 147 411 2378
Fragments arranged by size:
2816 2378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3195 3225 3519 3549 1178
Size: 30 165 30 1792 30 294 30 4053
Fragments arranged by size:
4053 1792 294 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2052 1186
Size: 866 5558
BseYI C'CCAG_C
Cuts at: 2330 5627 6123 2330
Size: 3297 496 2631
Fragments arranged by size:
3297 2631 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2991 1163
Size: 573 388 867 4596
Fragments arranged by size:
4596 867 573 388
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3874 4635 5663 1540
Size: 275 186 1873 761 1028 2301
Fragments arranged by size:
2301 1873 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4485 4570 5641 4485
Size: 85 1071 5268
Fragments arranged by size:
5268 1071 85
BsmI GAATG_Cn'
Cuts at: 3345 3454 3935 4028 3345
Size: 109 481 93 5741
Fragments arranged by size:
5741 481 109 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3334 3743 4485 4570 5641 1391
Size: 399 1544 409 742 85 1071 2174
Fragments arranged by size:
2174 1544 1071 742 409 399 85
BspEI T'CCGG_A
Cuts at: 6087 6087
Size: 6424
BspHI T'CATG_A
Cuts at: 3022 4313 6043 3022
Size: 1291 1730 3403
Fragments arranged by size:
3403 1730 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2788 3443 3779 844
Size: 1944 655 336 3489
Fragments arranged by size:
3489 1944 655 336
BsrBI CCG'CTC
Cuts at: 1394 1823 4311 1394
Size: 429 2488 3507
Fragments arranged by size:
3507 2488 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 3438 3690 4919 5093 65
Size: 1183 1329 861 252 1229 174 1396
Fragments arranged by size:
1396 1329 1229 1183 861 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 5068 5255 1700
Size: 110 231 3027 187 2869
Fragments arranged by size:
3027 2869 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6424
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6424
BssSI C'ACGA_G
Cuts at: 4484 5496 4484
Size: 1012 5412
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2516 794
Size: 1722 4702
BstBI TT'CG_AA
Cuts at: 5228 5228
Size: 6424
BstEII G'GTnAC_C
Cuts at: 1184 2013 1184
Size: 829 5595
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 6424
BstYI r'GATC_y
Cuts at: 1972 3464 3754 4121 4506 4523 5975 6061 6073
Size: 1492 290 367 385 17 1452 86 12
Cuts at: 6073 6419 1972
Size: 346 1977
Fragments arranged by size:
1977 1492 1452 385 367 346 290 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5872
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5685
Fragments arranged by size:
5685 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 2979 3940 4734 4754 2346
Size: 633 961 794 20 4016
Fragments arranged by size:
4016 961 794 633 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6389
DraI TTT'AAA
Cuts at: 3728 3728
Size: 6424
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5431 809
Size: 507 4115 1802
Fragments arranged by size:
4115 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 3871 4760
Size: 54 1425 50 275 18 327 1714 889
Cuts at: 4760 6389 8
Size: 1629 43
Fragments arranged by size:
1714 1629 1425 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3871 1537
Size: 275 2059 4090
Fragments arranged by size:
4090 2059 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2979 3654 4354 1353
Size: 1626 675 700 3423
Fragments arranged by size:
3423 1626 700 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5011 5385 5531 387
Size: 1064 138 3422 374 146 1280
Fragments arranged by size:
3422 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2520 2850 2873 2895 3236 3354 3546
Size: 393 888 330 23 22 341 118 192
Cuts at: 3546 4487 5084 5871 6139 1239
Size: 941 597 787 268 1524
Fragments arranged by size:
1524 941 888 787 597 393 341 330
268 192 118 23 22
EcoO109I rG'GnC_Cy
Cuts at: 3667 3667
Size: 6424
EcoRI G'AATT_C
Cuts at: 2717 3733 4111 2717
Size: 1016 378 5030
Fragments arranged by size:
5030 1016 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6424
FspI TGC'GCA
Cuts at: 4930 4930
Size: 6424
HaeII r_GCGC'y
Cuts at: 1678 5571 1678
Size: 3893 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2986 3018 5065 5097 5139
Size: 32 135 32 1414 32 2047 32 42
Cuts at: 5139 5171 1373
Size: 32 2626
Fragments arranged by size:
2626 2047 1414 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3621 3768 4014 669
Size: 988 281 1683 147 246 3079
Fragments arranged by size:
3079 1683 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6424
HpaI GTT'AAC
Cuts at: 3621 4014 3621
Size: 393 6031
KpnI G_GTAC'C
Cuts at: 3749 3749
Size: 6424
MfeI C'AATT_G
Cuts at: 2253 4023 2253
Size: 1770 4654
MluI A'CGCG_T
Cuts at: 6300 6300
Size: 6424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 3019 5538 5722 1305
Size: 1258 456 2519 184 2007
Fragments arranged by size:
2519 2007 1258 456 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4602
Fragments arranged by size:
4602 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4382 4741 4900 518
Size: 648 3216 359 159 2042
Fragments arranged by size:
3216 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3840 4517 5665 5910 1606
Size: 2234 677 1148 245 2120
Fragments arranged by size:
2234 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5872
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6424
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6424
NotI GC'GGCC_GC
Cuts at: 3871 3871
Size: 6424
NsiI A_TGCA'T
Cuts at: 3347 3347
Size: 6424
NspI r_CATG'y
Cuts at: 1132 1229 3721 3782 5327 1132
Size: 97 2492 61 1545 2229
Fragments arranged by size:
2492 2229 1545 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6424
PciI A'CATG_T
Cuts at: 5323 5323
Size: 6424
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2918 1108
Size: 1810 4614
PfoI T'CCnGG_A
Cuts at: 2721 2721
Size: 6424
PmeI GTTT'AAAC
Cuts at: 3728 3728
Size: 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 6006 6038 4493
Size: 32 1481 32 4879
Fragments arranged by size:
4879 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3667 3667
Size: 6424
PshAI GACnn'nnGTC
Cuts at: 1613 2044 1613
Size: 431 5993
PsiI TTA'TAA
Cuts at: 3994 3994
Size: 6424
PstI C_TGCA'G
Cuts at: 830 3776 830
Size: 2946 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6424
SalI G'TCGA_C
Cuts at: 1936 3766 1936
Size: 1830 4594
SbfI CC_TGCA'GG
Cuts at: 3776 3776
Size: 6424
ScaI AGT'ACT
Cuts at: 4672 6384 4672
Size: 1712 4712
SfcI C'TryA_G
Cuts at: 826 1045 2370 3624 3772 4907 5588 5779 826
Size: 219 1325 1254 148 1135 681 191 1471
Fragments arranged by size:
1471 1325 1254 1135 681 219 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6424
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6424
SmaI CCC'GGG
Cuts at: 1872 3751 1872
Size: 1879 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4528 5429 5691 820
Size: 197 926 2585 901 262 1553
Fragments arranged by size:
2585 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4215 493
Size: 3722 2702
SpeI A'CTAG_T
Cuts at: 152 3149 152
Size: 2997 3427
SphI G_CATG'C
Cuts at: 1132 3782 1132
Size: 2650 3774
SspI AAT'ATT
Cuts at: 5 52 4348 5
Size: 47 4296 2081
Fragments arranged by size:
4296 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6424
StyI C'CwwG_G
Cuts at: 513 1065 2376 3354 3863 513
Size: 552 1311 978 509 3074
Fragments arranged by size:
3074 1311 978 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3677 4451 4468 4621 4806 5251
Size: 1255 2088 9 774 17 153 185 445
Cuts at: 5251 325
Size: 1498
Fragments arranged by size:
2088 1498 1255 774 445 185 153 17
9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2356 2855 4670
Size: 274 80 33 51 1443 379 499 1815
Cuts at: 4670 6382 96
Size: 1712 138
Fragments arranged by size:
1815 1712 1443 499 379 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2172 2204 3195 3227 3519 3551 3634 3666 6384
Size: 32 991 32 292 32 83 32 2718
Cuts at: 6384 6416 2172
Size: 32 2180
Fragments arranged by size:
2718 2180 991 292 83 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6424
XbaI T'CTAG_A
Cuts at: 3760 3760
Size: 6424
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6424
XmaI C'CCGG_G
Cuts at: 1870 3749 1870
Size: 1879 4545
XmnI GAAnn'nnTTC
Cuts at: 3012 4553 3012
Size: 1541 4883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4232 276
Size: 53 83 186 936 2698 2468
Fragments arranged by size:
2698 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI