Restriction map for
pFN21AB5557
(Circular) (Six-base) MAPSORT of: pFN21AB5557.seq Check: 7219 from: 1 to: 6097
pFN21AB5557 6097 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3229 4160 5544 5812 1632
Size: 1597 931 1384 268 1917
Fragments arranged by size:
1917 1597 1384 931 268
AfeI AGC'GCT
Cuts at: 3204 3287 3204
Size: 83 6014
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2457 2483 4996 5973 1203
Size: 1254 26 2513 977 1327
Fragments arranged by size:
2513 1327 1254 977 26
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 3131 1166
Size: 1965 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaI G_GGCC'C
Cuts at: 2218 2218
Size: 6097
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2646 2759 3406 3632 3784 5893 1493
Size: 1153 113 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 1153 647 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3422 1870
Size: 73 964 515 4545
Fragments arranged by size:
4545 964 515 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 3418 4873 618
Size: 325 217 627 624 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2218 2892 2949 3416 1391
Size: 624 203 674 57 467 4072
Fragments arranged by size:
4072 674 624 467 203 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 928
Size: 971 231 4895
Fragments arranged by size:
4895 971 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2222 2447 3989 5199 2222
Size: 225 1542 1210 3120
Fragments arranged by size:
3120 1542 1210 225
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2277 4708 136
Size: 107 122 71 631 605 605 2431 1525
Fragments arranged by size:
2431 1525 631 605 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2218 2558 2753 4158 5314 1790
Size: 428 340 195 1405 1156 2573
Fragments arranged by size:
2573 1405 1156 428 340 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2524 2815 2876 3059 3177 4757 1239
Size: 1285 291 61 183 118 1580 2579
Fragments arranged by size:
2579 1580 1285 291 183 118 61
Bpu10I CC'TnA_GC
Cuts at: 3212 3212
Size: 6097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2205 3364 4222 5087 5385 2205
Size: 1159 858 865 298 2917
Fragments arranged by size:
2917 1159 865 858 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2697 4760 882
Size: 617 1198 2063 2219
Fragments arranged by size:
2219 2063 1198 617
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 1178
Size: 30 165 30 1005 30 4837
Fragments arranged by size:
4837 1005 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 1186
Size: 1834 4263
BseYI C'CCAG_C
Cuts at: 2084 5300 5796 2084
Size: 3216 496 2385
Fragments arranged by size:
3216 2385 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2976 2979 1163
Size: 573 1240 3 4281
Fragments arranged by size:
4281 1240 573 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 3547 4308 5336 1540
Size: 275 186 511 1035 761 1028 2301
Fragments arranged by size:
2301 1035 1028 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 2831 2892 4158 4243 5314 2177
Size: 654 61 1266 85 1071 2960
Fragments arranged by size:
2960 1266 1071 654 85 61
BsmI GAATG_Cn'
Cuts at: 3090 3608 3701 3090
Size: 518 93 5486
Fragments arranged by size:
5486 518 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2177 2218 2558 2753 2831 2892
Size: 399 225 162 41 340 195 78 61
Cuts at: 2892 2949 3416 4158 4243 5314 1391
Size: 57 467 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 340 225 195
162 85 78 61 57 41
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2473 2508 3984 1394
Size: 429 650 35 1476 3507
Fragments arranged by size:
3507 1476 650 429 35
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 4592 4766 65
Size: 1183 1326 2018 174 1396
Fragments arranged by size:
2018 1396 1326 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2511 3069 4741 4928 1700
Size: 110 701 558 1672 187 2869
Fragments arranged by size:
2869 1672 701 558 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2712 3427 3794 4179 4196 5648 5734 5746
Size: 740 715 367 385 17 1452 86 12
Cuts at: 5746 6092 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 740 715 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2168 3129 513
Size: 552 939 164 961 3481
Fragments arranged by size:
3481 961 939 552 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2374 487
Size: 683 56 1148 4210
Fragments arranged by size:
4210 1148 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2770 2805 529
Size: 35 2206 35 3821
Fragments arranged by size:
3821 2206 35 35
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DraIII CAC_nnn'GTG
Cuts at: 3104 3104
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 5104 809
Size: 507 816 2972 1802
Fragments arranged by size:
2972 1802 816 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 2513 3544
Size: 54 1425 50 275 18 679 4 1031
Cuts at: 3544 4433 6062 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 679 275 54 50
43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2509 3544 1537
Size: 275 697 1035 4090
Fragments arranged by size:
4090 1035 697 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2302 2623 3348 4027 1353
Size: 949 321 725 679 3423
Fragments arranged by size:
3423 949 725 679 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2890 2890
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2524 2815 2876 3059 3177 3229 4160
Size: 393 892 291 61 183 118 52 931
Cuts at: 4160 4757 5544 5812 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 931 892 787 597 393 291 268
183 118 61 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2223 2223
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2214 2215 2214
Size: 1 6096
EcoRI G'AATT_C
Cuts at: 2646 3406 3784 2646
Size: 760 378 4959
Fragments arranged by size:
4959 760 378
FseI GG_CCGG'CC
Cuts at: 2515 2515
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 3206 3289 5244 1678
Size: 1528 83 1955 2531
Fragments arranged by size:
2531 1955 1528 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3175 3207 4738 4770 4812
Size: 32 135 32 1603 32 1531 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 1603 1531 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3441 3687 669
Size: 988 281 1503 246 3079
Fragments arranged by size:
3079 1503 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2712 2946 5211 5395 1305
Size: 1407 234 2265 184 2007
Fragments arranged by size:
2265 2007 1407 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2486 3128 3131 4055 4414 4573
Size: 648 1020 300 642 3 924 359 159
Cuts at: 4573 518
Size: 2042
Fragments arranged by size:
2042 1020 924 648 642 359 300 159
3
MspA1I CmG'CkG
Cuts at: 1606 2993 3513 4190 5338 5583 1606
Size: 1387 520 677 1148 245 2120
Fragments arranged by size:
2120 1387 1148 677 520 245
NaeI GCC'GGC
Cuts at: 2513 2513
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4606
Fragments arranged by size:
4606 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NgoMIV G'CCGG_C
Cuts at: 2511 2511
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 2487 3455 5000 1132
Size: 97 1258 968 1545 2229
Fragments arranged by size:
2229 1545 1258 968 97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 5200
PciI A'CATG_T
Cuts at: 2483 4996 2483
Size: 2513 3584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2387 2813 1108
Size: 1279 426 4392
Fragments arranged by size:
4392 1279 426
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2214 2214
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2213 3093 3210 3449 830
Size: 1383 880 117 239 3478
Fragments arranged by size:
3478 1383 880 239 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2993 2993
Size: 6097
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 6097
SacI G_AGCT'C
Cuts at: 2892 2892
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SapI GCTCTTCn'nnn_
Cuts at: 2302 2302
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SexAI A'CCwGG_T
Cuts at: 2252 2252
Size: 6097
SfcI C'TryA_G
Cuts at: 826 1045 2209 3089 3206 3445 4580 5261 5452
Size: 219 1164 880 117 239 1135 681 191
Cuts at: 5452 826
Size: 1471
Fragments arranged by size:
1471 1164 1135 880 681 239 219 191
117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2220 3343 4201 5102 5364 820
Size: 197 926 277 1123 858 901 262 1553
Fragments arranged by size:
1553 1123 926 901 858 277 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 2501 2673 1517
Size: 984 172 4941
Fragments arranged by size:
4941 984 172
StyI C'CwwG_G
Cuts at: 513 1065 2004 3536 513
Size: 552 939 1532 3074
Fragments arranged by size:
3074 1532 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 3061 3134 4124 4141 4294 4479
Size: 1255 1386 95 73 990 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1386 1255 990 445 185 153 95
73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 2718 4343
Size: 274 80 33 51 1443 228 513 1625
Cuts at: 4343 6055 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 513 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 2339 1316
Size: 1023 5074
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI SacII SanDI SfiI SfoI SrfI
SwaI XcmI