Restriction map for
pFN21AB5551
(Circular) (Six-base) MAPSORT of: pFN21AB5551.seq Check: 7681 from: 1 to: 6745
pFN21AB5551 6745 bp
With 182 enzymes: SgfI *
May 22, 2017 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3843 3843
Size: 6745
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3045 4555 278
Size: 53 83 186 936 1509 1510 2468
Fragments arranged by size:
2468 1510 1509 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3822 4088 1937
Size: 1885 266 4594
Fragments arranged by size:
4594 1885 266
Acc65I G'GTAC_C
Cuts at: 4066 4066
Size: 6745
AclI AA'CG_TT
Cuts at: 1563 4872 5245 1563
Size: 3309 373 3063
Fragments arranged by size:
3309 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 2831 4808 6192 6460 1632
Size: 496 703 1977 1384 268 1917
Fragments arranged by size:
1977 1917 1384 703 496 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 4035 5644 6621 1203
Size: 2832 1609 977 1327
Fragments arranged by size:
2832 1609 1327 977
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 3316 5474 3316
Size: 2158 4587
AleI CACnn'nnGTG
Cuts at: 1166 3345 3426 3620 1166
Size: 2179 81 194 4291
Fragments arranged by size:
4291 2179 194 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2574 3444 1184
Size: 1390 870 4485
Fragments arranged by size:
4485 1390 870
AlwNI CAG_nnn'CTG
Cuts at: 3214 3835 4488 6060 3214
Size: 621 653 1572 3899
Fragments arranged by size:
3899 1572 653 621
ApaI G_GGCC'C
Cuts at: 3611 3611
Size: 6745
ApaLI G'TGCA_C
Cuts at: 3419 4802 5958 3419
Size: 1383 1156 4206
Fragments arranged by size:
4206 1383 1156
ApoI r'AATT_y
Cuts at: 1493 2181 4054 4280 4432 6541 1493
Size: 688 1873 226 152 2109 1697
Fragments arranged by size:
2109 1873 1697 688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 3801 3962 4070 1870
Size: 73 225 1633 161 108 4545
Fragments arranged by size:
4545 1633 225 161 108 73
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2600 3029 3538 3614 3625
Size: 325 217 627 813 429 509 76 11
Cuts at: 3625 3881 4066 5521 618
Size: 256 185 1455 1842
Fragments arranged by size:
1842 1455 813 627 509 429 325 256
217 185 76 11
BanII G_rGCy'C
Cuts at: 1391 3201 3611 3963 4064 1391
Size: 1810 410 352 101 4072
Fragments arranged by size:
4072 1810 410 352 101
BbeI G_GCGC'C
Cuts at: 2604 2604
Size: 6745
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2756 2790 3863 3897 4936 4970 1903
Size: 34 819 34 1073 34 1039 34 3678
Fragments arranged by size:
3678 1073 1039 819 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4637 5847 4637
Size: 1210 5535
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5356 136
Size: 107 122 71 631 605 3684 1525
Fragments arranged by size:
3684 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6740 6740
Size: 6745
BlpI GC'TnA_GC
Cuts at: 3957 4162 3957
Size: 205 6540
Bme1580I G_kGCm'C
Cuts at: 1790 3104 3423 3543 3611 3886 4806 5962 1790
Size: 1314 319 120 68 275 920 1156 2573
Fragments arranged by size:
2573 1314 1156 920 319 275 120 68
BmgBI CAC'GTC
Cuts at: 1318 2494 4036 1318
Size: 1176 1542 4027
Fragments arranged by size:
4027 1542 1176
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3556 4476 5434
Size: 416 53 83 399 24 2139 920 958
Cuts at: 5434 442
Size: 1753
Fragments arranged by size:
2139 1753 958 920 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 5405 1239
Size: 1370 2796 2579
Fragments arranged by size:
2796 2579 1370
Bpu10I CC'TnA_GC
Cuts at: 2378 3263 2378
Size: 885 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4870 5735 6033 4870
Size: 865 298 5582
Fragments arranged by size:
5582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2126 2436 3443 5408 882
Size: 617 627 310 1007 1965 2219
Fragments arranged by size:
2219 1965 1007 627 617 310
BsaAI yAC'GTr
Cuts at: 493 1204 2029 2575 2971 4536 493
Size: 711 825 546 396 1565 2702
Fragments arranged by size:
2702 1565 825 711 546 396
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2601 3042 4552
Size: 53 83 186 936 783 285 441 1510
Cuts at: 4552 4934 5569 275
Size: 382 635 1451
Fragments arranged by size:
1510 1451 936 783 635 441 382 285
186 83 53
BsaWI w'CCGG_w
Cuts at: 2880 5178 5576 5850 5997 6408 2880
Size: 2298 398 274 147 411 3217
Fragments arranged by size:
3217 2298 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 1178
Size: 30 165 30 621 30 5869
Fragments arranged by size:
5869 621 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2188 2303 2372 2666 2741 3086 3223 3226
Size: 1002 115 69 294 75 345 137 3
Cuts at: 3226 3758 1186
Size: 532 4173
Fragments arranged by size:
4173 1002 532 345 294 137 115 75
69 3
BseYI C'CCAG_C
Cuts at: 2604 2620 2983 3013 3267 3780 5948 6444 2604
Size: 16 363 30 254 513 2168 496 2905
Fragments arranged by size:
2905 2168 513 496 363 254 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3950 1163
Size: 573 2214 3958
Fragments arranged by size:
3958 2214 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2691 3463 3529 4195 4956 5984
Size: 275 186 690 772 66 666 761 1028
Cuts at: 5984 1540
Size: 2301
Fragments arranged by size:
2301 1028 772 761 690 666 275 186
66
BsiHKAI G_wGCw'C
Cuts at: 2553 3423 3858 3963 3978 4806 4891 5962 2553
Size: 870 435 105 15 828 85 1071 3336
Fragments arranged by size:
3336 1071 870 828 435 105 85 15
BsmI GAATG_Cn'
Cuts at: 2736 4256 4349 2736
Size: 1520 93 5132
Fragments arranged by size:
5132 1520 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3683 3683
Size: 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2553 3104 3201 3423 3543 3611 3858
Size: 399 763 551 97 222 120 68 247
Cuts at: 3858 3886 3963 3978 4064 4806 4891 5962 1391
Size: 28 77 15 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 763 742 551 399 247 222
120 97 86 85 77 68 28 15
BspEI T'CCGG_A
Cuts at: 6408 6408
Size: 6745
BspHI T'CATG_A
Cuts at: 4634 6364 4634
Size: 1730 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3843 4100 844
Size: 2999 257 3489
Fragments arranged by size:
3489 2999 257
BsrBI CCG'CTC
Cuts at: 1394 1823 3652 3717 4632 1394
Size: 429 1829 65 915 3507
Fragments arranged by size:
3507 1829 915 429 65
BsrDI GCAATG_nn'
Cuts at: 65 1248 5240 5414 65
Size: 1183 3992 174 1396
Fragments arranged by size:
3992 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2686 2805 3458 5389 5576 1700
Size: 110 876 119 653 1931 187 2869
Fragments arranged by size:
2869 1931 876 653 187 119 110
BsrGI T'GTAC_A
Cuts at: 96 2787 96
Size: 2691 4054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6745
BssSI C'ACGA_G
Cuts at: 4805 5817 4805
Size: 1012 5733
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3214 794
Size: 2420 4325
BstBI TT'CG_AA
Cuts at: 5549 5549
Size: 6745
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6745
BstYI r'GATC_y
Cuts at: 1972 2497 4075 4442 4827 4844 6296 6382 6394
Size: 525 1578 367 385 17 1452 86 12
Cuts at: 6394 6740 1972
Size: 346 1977
Fragments arranged by size:
1977 1578 1452 525 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2355 2355
Size: 6745
BtgI C'CryG_G
Cuts at: 513 1065 2562 3969 513
Size: 552 1497 1407 3289
Fragments arranged by size:
3289 1497 1407 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3370 487
Size: 683 56 2144 3862
Fragments arranged by size:
3862 2144 683 56
BtsI GCAGTG_nn'
Cuts at: 4261 5055 5075 4261
Size: 794 20 5931
Fragments arranged by size:
5931 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6710
DraI TTT'AAA
Cuts at: 4049 4049
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3041 5752 809
Size: 507 1725 2711 1802
Fragments arranged by size:
2711 1802 1725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2370 2424
Size: 54 1425 50 275 18 231 309 54
Cuts at: 2424 2684 2688 2778 3297 3460 3480 3526 3918
Size: 260 4 90 519 163 20 46 392
Cuts at: 3918 4192 5081 6710 8
Size: 274 889 1629 43
Fragments arranged by size:
1629 1425 889 519 392 309 275 274
260 231 163 90 54 54 50 46
43 20 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2688 3460 3526 4192 1537
Size: 275 876 772 66 666 4090
Fragments arranged by size:
4090 876 772 666 275 66
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 2807 4675 1353
Size: 794 660 1868 3423
Fragments arranged by size:
3423 1868 794 660
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3557 5332 5706 5852 387
Size: 1064 138 1968 1775 374 146 1280
Fragments arranged by size:
1968 1775 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3961 3961
Size: 6745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2609 2831 4808 5405 6192 6460
Size: 393 496 481 222 1977 597 787 268
Cuts at: 6460 1239
Size: 1524
Fragments arranged by size:
1977 1524 787 597 496 481 393 268
222
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 3068 2309
Size: 759 5986
EcoO109I rG'GnC_Cy
Cuts at: 3608 3608
Size: 6745
EcoRI G'AATT_C
Cuts at: 4054 4432 4054
Size: 378 6367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 2854 2093
Size: 761 5984
FseI GG_CCGG'CC
Cuts at: 2690 3462 2690
Size: 772 5973
FspI TGC'GCA
Cuts at: 3221 3554 5251 3221
Size: 333 1697 4715
Fragments arranged by size:
4715 1697 333
HaeII r_GCGC'y
Cuts at: 1678 2604 2908 5892 1678
Size: 926 304 2984 2531
Fragments arranged by size:
2984 2531 926 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 3059 3091 3302
Size: 32 135 32 452 32 1003 32 211
Cuts at: 3302 3334 5386 5418 5460 5492 1373
Size: 32 2052 32 42 32 2626
Fragments arranged by size:
2626 2052 1003 452 211 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 4089 4335 669
Size: 988 281 1069 1082 246 3079
Fragments arranged by size:
3079 1082 1069 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 4335 4335
Size: 6745
KasI G'GCGC_C
Cuts at: 2600 2600
Size: 6745
KpnI G_GTAC'C
Cuts at: 4070 4070
Size: 6745
MfeI C'AATT_G
Cuts at: 4344 4344
Size: 6745
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 5859 6043 1305
Size: 1797 2757 184 2007
Fragments arranged by size:
2757 2007 1797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 3299 3482 3920 10
Size: 54 1425 343 594 873 183 438 2835
Fragments arranged by size:
2835 1425 873 594 438 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2556 3097 3345 3426 3548 3602
Size: 648 1089 301 541 248 81 122 54
Cuts at: 3602 3620 4703 5062 5221 518
Size: 18 1083 359 159 2042
Fragments arranged by size:
2042 1089 1083 648 541 359 301 248
159 122 81 54 18
MspA1I CmG'CkG
Cuts at: 1606 2348 3013 4161 4838 5986 6231 1606
Size: 742 665 1148 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 742 677 665 245
NaeI GCC'GGC
Cuts at: 2688 2807 3460 2688
Size: 119 653 5973
Fragments arranged by size:
5973 653 119
NarI GG'CG_CC
Cuts at: 2601 2601
Size: 6745
NcoI C'CATG_G
Cuts at: 513 1065 2562 3969 513
Size: 552 1497 1407 3289
Fragments arranged by size:
3289 1497 1407 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6745
NgoMIV G'CCGG_C
Cuts at: 2686 2805 3458 2686
Size: 119 653 5973
Fragments arranged by size:
5973 653 119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 4192 4192
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 2344 4103 5648 1132
Size: 97 1115 1759 1545 2229
Fragments arranged by size:
2229 1759 1545 1115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6745
PciI A'CATG_T
Cuts at: 5644 5644
Size: 6745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3489 1108
Size: 2381 4364
PfoI T'CCnGG_A
Cuts at: 3645 3645
Size: 6745
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PmlI CAC'GTG
Cuts at: 2029 2971 2029
Size: 942 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4814 4846 6327 6359 4814
Size: 32 1481 32 5200
Fragments arranged by size:
5200 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6745
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PspOMI G'GGCC_C
Cuts at: 3607 3607
Size: 6745
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2528 2560 3926 3958 2528
Size: 32 1366 32 5315
Fragments arranged by size:
5315 1366 32 32
PstI C_TGCA'G
Cuts at: 830 2287 2484 2869 3309 3369 4097 830
Size: 1457 197 385 440 60 728 3478
Fragments arranged by size:
3478 1457 728 440 385 197 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
PvuII CAG'CTG
Cuts at: 3013 3013
Size: 6745
SacI G_AGCT'C
Cuts at: 3963 3963
Size: 6745
SalI G'TCGA_C
Cuts at: 1936 4087 1936
Size: 2151 4594
SapI GCTCTTCn'nnn_
Cuts at: 2807 2807
Size: 6745
SbfI CC_TGCA'GG
Cuts at: 3309 3369 4097 3309
Size: 60 728 5957
Fragments arranged by size:
5957 728 60
ScaI AGT'ACT
Cuts at: 4993 6705 4993
Size: 1712 5033
SfcI C'TryA_G
Cuts at: 826 1045 2283 2480 2865 3305 3365 4093 5228
Size: 219 1238 197 385 440 60 728 1135
Cuts at: 5228 5909 6100 826
Size: 681 191 1471
Fragments arranged by size:
1471 1238 1135 728 681 440 385 219
197 191 60
SfoI GGC'GCC
Cuts at: 2602 2602
Size: 6745
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6745
SmaI CCC'GGG
Cuts at: 1872 3803 4072 1872
Size: 1931 269 4545
Fragments arranged by size:
4545 1931 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4849 5750 6012 820
Size: 197 926 2906 901 262 1553
Fragments arranged by size:
2906 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4536 493
Size: 4043 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 4103 1132
Size: 2971 3774
SspI AAT'ATT
Cuts at: 5 52 4669 5
Size: 47 4617 2081
Fragments arranged by size:
4617 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6745
StyI C'CwwG_G
Cuts at: 513 1065 2310 2562 2781 3399 3453 3501 3969
Size: 552 1245 252 219 618 54 48 468
Cuts at: 3969 4014 4184 513
Size: 45 170 3074
Fragments arranged by size:
3074 1245 618 552 468 252 219 170
54 48 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3105 3259 3794 3864 4772 4789
Size: 1255 1437 88 154 535 70 908 17
Cuts at: 4789 4942 5127 5572 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 908 535 445 185 154
153 88 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 3699 4991
Size: 274 80 33 51 1443 810 912 1292
Cuts at: 4991 6703 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1292 912 810 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6705 6737 6705
Size: 32 6713
Tth111I GACn'n_nGTC
Cuts at: 1316 3044 1316
Size: 1728 5017
XbaI T'CTAG_A
Cuts at: 4081 4081
Size: 6745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2788 2011
Size: 777 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6745
XmaI C'CCGG_G
Cuts at: 1870 3801 4070 1870
Size: 1931 269 4545
Fragments arranged by size:
4545 1931 269
XmnI GAAnn'nnTTC
Cuts at: 2855 3938 4874 2855
Size: 1083 936 4726
Fragments arranged by size:
4726 1083 936
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3043 4553 276
Size: 53 83 186 936 1509 1510 2468
Fragments arranged by size:
2468 1510 1509 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FspAI NruI NsiI
PacI PpuMI PspXI RsrII SacII SanDI SexAI SfiI
SrfI SwaI