Restriction map for
pFN21AB5550
(Circular) (Six-base) MAPSORT of: pFN21AB5550.seq Check: 6861 from: 1 to: 6667
pFN21AB5550 6667 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4477 278
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 4010 1937
Size: 1077 996 4594
Fragments arranged by size:
4594 1077 996
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 4794 5167 1563
Size: 3231 373 3063
Fragments arranged by size:
3231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2235 2451 3564 3630 3789 4730 6114 6382
Size: 603 216 1113 66 159 941 1384 268
Cuts at: 6382 1632
Size: 1917
Fragments arranged by size:
1917 1384 1113 941 603 268 216 159
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 5566 6543 1203
Size: 4363 977 1327
Fragments arranged by size:
4363 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 5396 5396
Size: 6667
AleI CACnn'nnGTG
Cuts at: 1166 3329 1166
Size: 2163 4504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2327 2359 2327
Size: 32 6635
AlwNI CAG_nnn'CTG
Cuts at: 3124 3368 4410 5982 3124
Size: 244 1042 1572 3809
Fragments arranged by size:
3809 1572 1042 244
ApaI G_GGCC'C
Cuts at: 2899 2899
Size: 6667
ApaLI G'TGCA_C
Cuts at: 4724 5880 4724
Size: 1156 5511
ApoI r'AATT_y
Cuts at: 1493 2436 2981 3105 3462 3976 4202 4354 6463
Size: 943 545 124 357 514 226 152 2109
Cuts at: 6463 1493
Size: 1697
Fragments arranged by size:
2109 1697 943 545 514 357 226 152
124
AseI AT'TA_AT
Cuts at: 160 1032 5221 160
Size: 872 4189 1606
Fragments arranged by size:
4189 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 2267 2973 3992 1870
Size: 73 324 706 1019 4545
Fragments arranged by size:
4545 1019 706 324 73
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3331 3988 5443 618
Size: 325 217 627 1544 657 1455 1842
Fragments arranged by size:
1842 1544 1455 657 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2899 2994 3986 1391
Size: 1508 95 992 4072
Fragments arranged by size:
4072 1508 992 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2719 2778 2828 2895 2958 3552 3663
Size: 971 820 59 50 67 63 594 111
Cuts at: 3663 3852 928
Size: 189 3743
Fragments arranged by size:
3743 971 820 594 189 111 67 63
59 50
BbvCI CC'TCA_GC
Cuts at: 2475 3651 2475
Size: 1176 5491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2017 2051 2077 2111 4858 4892 1903
Size: 34 80 34 26 34 2747 34 3678
Fragments arranged by size:
3678 2747 80 34 34 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 3937 4559 5769 3937
Size: 622 1210 4835
Fragments arranged by size:
4835 1210 622
BclI T'GATC_A
Cuts at: 1548 3226 1548
Size: 1678 4989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2894 5278 136
Size: 107 122 71 631 605 1222 2384 1525
Fragments arranged by size:
2384 1525 1222 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3351 6662 3351
Size: 3311 3356
BlpI GC'TnA_GC
Cuts at: 2042 4084 2042
Size: 2042 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2899 4728 5884 1790
Size: 300 809 1829 1156 2573
Fragments arranged by size:
2573 1829 1156 809 300
BmgBI CAC'GTC
Cuts at: 1318 2083 1318
Size: 765 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4398 5356 442
Size: 416 53 83 399 24 2981 958 1753
Fragments arranged by size:
2981 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5327 1239
Size: 4088 2579
Bpu10I CC'TnA_GC
Cuts at: 2475 3651 2475
Size: 1176 5491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3653 4792 5657 5955 3653
Size: 1139 865 298 4365
Fragments arranged by size:
4365 1139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2957 3773 5330 882
Size: 617 1458 816 1557 2219
Fragments arranged by size:
2219 1557 1458 816 617
BsaAI yAC'GTr
Cuts at: 493 1204 2310 4458 493
Size: 711 1106 2148 2702
Fragments arranged by size:
2702 2148 1106 711
BsaBI GATnn'nnATC
Cuts at: 2757 3578 6661 2757
Size: 821 3083 2763
Fragments arranged by size:
3083 2763 821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 4474 4856 5491
Size: 53 83 186 936 753 2188 382 635
Cuts at: 5491 275
Size: 1451
Fragments arranged by size:
2188 1451 936 753 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3009 5100 5498 5772 5919 6330 3009
Size: 2091 398 274 147 411 3346
Fragments arranged by size:
3346 2091 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2410 2440 1178
Size: 30 165 30 1007 30 5405
Fragments arranged by size:
5405 1007 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 3465 1186
Size: 889 3 1387 4388
Fragments arranged by size:
4388 1387 889 3
BseYI C'CCAG_C
Cuts at: 3748 5870 6366 3748
Size: 2122 496 4049
Fragments arranged by size:
4049 2122 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2879 3936 1163
Size: 573 612 531 1057 3894
Fragments arranged by size:
3894 1057 612 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2149 2305 4117 4878 5906 1540
Size: 275 186 148 156 1812 761 1028 2301
Fragments arranged by size:
2301 1812 1028 761 275 186 156 148
BsiHKAI G_wGCw'C
Cuts at: 2994 3219 4728 4813 5884 2994
Size: 225 1509 85 1071 3777
Fragments arranged by size:
3777 1509 1071 225 85
BsmI GAATG_Cn'
Cuts at: 4178 4271 4178
Size: 93 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2899 2994 3219 3986 4728 4813
Size: 399 300 809 95 225 767 742 85
Cuts at: 4813 5884 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 809 767 742 399 300 225
95 85
BspEI T'CCGG_A
Cuts at: 6330 6330
Size: 6667
BspHI T'CATG_A
Cuts at: 3300 4556 6286 3300
Size: 1256 1730 3681
Fragments arranged by size:
3681 1730 1256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4022 844
Size: 3178 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4554 1394
Size: 429 2731 3507
Fragments arranged by size:
3507 2731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5162 5336 65
Size: 1183 3914 174 1396
Fragments arranged by size:
3914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5311 5498 1700
Size: 110 3501 187 2869
Fragments arranged by size:
3501 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2811 96
Size: 2715 3952
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6667
BssSI C'ACGA_G
Cuts at: 3477 4727 5739 3477
Size: 1250 1012 4405
Fragments arranged by size:
4405 1250 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6667
BstBI TT'CG_AA
Cuts at: 2730 5471 2730
Size: 2741 3926
BstEII G'GTnAC_C
Cuts at: 1184 2497 3239 1184
Size: 1313 742 4612
Fragments arranged by size:
4612 1313 742
BstYI r'GATC_y
Cuts at: 1972 3001 3351 3997 4364 4749 4766 6218 6304
Size: 1029 350 646 367 385 17 1452 86
Cuts at: 6304 6316 6662 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1029 646 385 367 350 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2445 3160 3783 513
Size: 552 1380 715 623 3397
Fragments arranged by size:
3397 1380 715 623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2042 2146 2250 487
Size: 683 56 816 104 104 4904
Fragments arranged by size:
4904 816 683 104 104 56
BtsI GCAGTG_nn'
Cuts at: 4183 4977 4997 4183
Size: 794 20 5853
Fragments arranged by size:
5853 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6632
DraI TTT'AAA
Cuts at: 3971 3971
Size: 6667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2723 2786 5674 809
Size: 507 1407 63 2888 1802
Fragments arranged by size:
2888 1802 1407 507 63
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2146 2302
Size: 54 1425 50 275 18 192 124 156
Cuts at: 2302 2739 4114 5003 6632 8
Size: 437 1375 889 1629 43
Fragments arranged by size:
1629 1425 1375 889 437 275 192 156
124 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2146 2302 4114 1537
Size: 275 334 156 1812 4090
Fragments arranged by size:
4090 1812 334 275 156
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 2982 3933 4597 1353
Size: 1356 273 951 664 3423
Fragments arranged by size:
3423 1356 951 664 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2700 5254 5628 5774 387
Size: 1064 138 1111 2554 374 146 1280
Fragments arranged by size:
2554 1280 1111 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2992 2992
Size: 6667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2451 3564 3630 3789 4730 5327
Size: 393 603 216 1113 66 159 941 597
Cuts at: 5327 6114 6382 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1113 941 787 603 597 393 268
216 159 66
EcoO109I rG'GnC_Cy
Cuts at: 2896 3484 3920 2896
Size: 588 436 5643
Fragments arranged by size:
5643 588 436
EcoRI G'AATT_C
Cuts at: 3105 3462 3976 4354 3105
Size: 357 514 378 5418
Fragments arranged by size:
5418 514 378 357
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 5814 1678
Size: 4136 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2956 2988 5308
Size: 32 135 32 838 32 514 32 2320
Cuts at: 5308 5340 5382 5414 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2320 838 514 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4011 4257 669
Size: 988 281 2073 246 3079
Fragments arranged by size:
3079 2073 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6667
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 4266 4266
Size: 6667
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 3021 3678 5781 5965 1305
Size: 981 735 657 2103 184 2007
Fragments arranged by size:
2103 2007 981 735 657 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 10
Size: 54 1425 343 909 3936
Fragments arranged by size:
3936 1425 909 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2666 2763 3329 3831 4625 4984
Size: 648 1293 207 97 566 502 794 359
Cuts at: 4984 5143 518
Size: 159 2042
Fragments arranged by size:
2042 1293 794 648 566 502 359 207
159 97
MspA1I CmG'CkG
Cuts at: 1606 2474 2618 3542 4083 4760 5908 6153 1606
Size: 868 144 924 541 677 1148 245 2120
Fragments arranged by size:
2120 1148 924 868 677 541 245 144
NcoI C'CATG_G
Cuts at: 513 1065 2445 3783 513
Size: 552 1380 1338 3397
Fragments arranged by size:
3397 1380 1338 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 4114 4114
Size: 6667
NspI r_CATG'y
Cuts at: 1132 1229 4025 5570 1132
Size: 97 2796 1545 2229
Fragments arranged by size:
2796 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6667
PciI A'CATG_T
Cuts at: 5566 5566
Size: 6667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3161 1108
Size: 2053 4614
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PmlI CAC'GTG
Cuts at: 2310 2310
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3484 3484
Size: 6667
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6667
PsiI TTA'TAA
Cuts at: 4237 4237
Size: 6667
PspOMI G'GGCC_C
Cuts at: 2895 2895
Size: 6667
PstI C_TGCA'G
Cuts at: 830 4019 830
Size: 3189 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 2618 3542 2618
Size: 924 5743
SacI G_AGCT'C
Cuts at: 2994 2994
Size: 6667
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SanDI GG'GwC_CC
Cuts at: 3484 3484
Size: 6667
SapI GCTCTTCn'nnn_
Cuts at: 2982 2982
Size: 6667
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 4915 6627 4915
Size: 1712 4955
SexAI A'CCwGG_T
Cuts at: 3243 3243
Size: 6667
SfcI C'TryA_G
Cuts at: 826 1045 3183 3360 3795 4015 5150 5831 6022
Size: 219 2138 177 435 220 1135 681 191
Cuts at: 6022 826
Size: 1471
Fragments arranged by size:
2138 1471 1135 681 435 220 219 191
177
SfiI GGCCn_nnn'nGGCC
Cuts at: 2894 2894
Size: 6667
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 3994 1872
Size: 2122 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3668 4771 5672 5934 820
Size: 197 926 1725 1103 901 262 1553
Fragments arranged by size:
1725 1553 1103 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4458 493
Size: 3965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 4025 1132
Size: 2893 3774
SspI AAT'ATT
Cuts at: 5 52 4591 5
Size: 47 4539 2081
Fragments arranged by size:
4539 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6667
StyI C'CwwG_G
Cuts at: 513 1065 2445 3317 3783 4106 513
Size: 552 1380 872 466 323 3074
Fragments arranged by size:
3074 1380 872 552 466 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2669 2753 4694 4711 4864 5049 5494
Size: 1255 1089 84 1941 17 153 185 445
Cuts at: 5494 325
Size: 1498
Fragments arranged by size:
1941 1498 1255 1089 445 185 153 84
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 4913 6625
Size: 274 80 33 51 1443 834 2102 1712
Cuts at: 6625 96
Size: 138
Fragments arranged by size:
2102 1712 1443 834 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6627 6659 6627
Size: 32 6635
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6667
XbaI T'CTAG_A
Cuts at: 4003 4003
Size: 6667
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6667
XmaI C'CCGG_G
Cuts at: 1870 3992 1870
Size: 2122 4545
XmnI GAAnn'nnTTC
Cuts at: 3081 3882 4796 3081
Size: 801 914 4952
Fragments arranged by size:
4952 914 801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4475 276
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacII SfoI
SrfI SwaI XcmI