Restriction map for
pFN21AB5549
(Circular) (Six-base) MAPSORT of: pFN21AB5549.seq Check: 495 from: 1 to: 8845
pFN21AB5549 8845 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3085 3085
Size: 8845
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6655 278
Size: 53 83 186 936 5119 2468
Fragments arranged by size:
5119 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4156 5544 6059 6188 1937
Size: 2219 1388 515 129 4594
Fragments arranged by size:
4594 2219 1388 515 129
Acc65I G'GTAC_C
Cuts at: 5799 6166 5799
Size: 367 8478
AclI AA'CG_TT
Cuts at: 1563 6972 7345 1563
Size: 5409 373 3063
Fragments arranged by size:
5409 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3381 3792 4971 5381 6135 6908 8292 8560
Size: 1749 411 1179 410 754 773 1384 268
Cuts at: 8560 1632
Size: 1917
Fragments arranged by size:
1917 1749 1384 1179 773 754 411 410
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8648
AflIII A'CryG_T
Cuts at: 1203 2529 6142 7744 8721 1203
Size: 1326 3613 1602 977 1327
Fragments arranged by size:
3613 1602 1327 1326 977
AgeI A'CCGG_T
Cuts at: 7676 7676
Size: 8845
AhdI GACnn_n'nnGTC
Cuts at: 7574 7574
Size: 8845
AleI CACnn'nnGTG
Cuts at: 1166 2528 3365 1166
Size: 1362 837 6646
Fragments arranged by size:
6646 1362 837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5853 1184
Size: 4669 4176
AlwNI CAG_nnn'CTG
Cuts at: 3188 3218 3422 3770 4538 6113 6588 8160 3188
Size: 30 204 348 768 1575 475 1572 3873
Fragments arranged by size:
3873 1575 1572 768 475 348 204 30
ApaI G_GGCC'C
Cuts at: 2361 2361
Size: 8845
ApaLI G'TGCA_C
Cuts at: 6902 8058 6902
Size: 1156 7689
ApoI r'AATT_y
Cuts at: 1493 3671 3801 3910 4135 5030 5248 6045 6154
Size: 2178 130 109 225 895 218 797 109
Cuts at: 6154 6380 6532 8641 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2178 2109 1697 895 797 226 225 218
152 130 109 109
AseI AT'TA_AT
Cuts at: 160 1032 3225 4461 7399 160
Size: 872 2193 1236 2938 1606
Fragments arranged by size:
2938 2193 1606 1236 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8845
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2711 4542 6170 1870
Size: 73 148 620 1831 1628 4545
Fragments arranged by size:
4545 1831 1628 620 148 73
BamHI G'GATC_C
Cuts at: 2736 6175 6542 2736
Size: 3439 367 5039
Fragments arranged by size:
5039 3439 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2707 5799 6166 7621
Size: 325 217 627 357 563 3092 367 1455
Cuts at: 7621 618
Size: 1842
Fragments arranged by size:
3092 1842 1455 627 563 367 357 325
217
BanII G_rGCy'C
Cuts at: 1391 2087 2361 5137 6164 1391
Size: 696 274 2776 1027 4072
Fragments arranged by size:
4072 2776 1027 696 274
BbeI G_GCGC'C
Cuts at: 2148 2148
Size: 8845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2949 928
Size: 971 1050 6824
Fragments arranged by size:
6824 1050 971
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 8845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7036 7070 1903
Size: 34 5099 34 3678
Fragments arranged by size:
5099 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4185 4991 5188 6737 7947 4185
Size: 806 197 1549 1210 5083
Fragments arranged by size:
5083 1549 1210 806 197
BclI T'GATC_A
Cuts at: 1548 3123 1548
Size: 1575 7270
BfrBI ATG'CAT
Cuts at: 2419 3694 2419
Size: 1275 7570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 2038 2096
Size: 107 122 71 631 605 363 3 58
Cuts at: 2096 2153 2235 2389 7456 136
Size: 57 82 154 5067 1525
Fragments arranged by size:
5067 1525 631 605 363 154 122 107
82 71 58 57 3
BglII A'GATC_T
Cuts at: 5609 8840 5609
Size: 3231 5614
BlpI GC'TnA_GC
Cuts at: 6262 6262
Size: 8845
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2575 2712 6906 8062 1790
Size: 571 214 137 4194 1156 2573
Fragments arranged by size:
4194 2573 1156 571 214 137
BmgBI CAC'GTC
Cuts at: 1318 2530 1318
Size: 1212 7633
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 4463 5412
Size: 416 53 83 399 24 1901 1145 949
Cuts at: 5412 6576 7534 442
Size: 1164 958 1753
Fragments arranged by size:
1901 1753 1164 1145 958 949 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3438 4668 1056
Size: 2382 1230 5233
Fragments arranged by size:
5233 2382 1230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2799 4509 7505 1239
Size: 1560 1710 2996 2579
Fragments arranged by size:
2996 2579 1710 1560
Bpu10I CC'TnA_GC
Cuts at: 2207 6014 6083 2207
Size: 3807 69 4969
Fragments arranged by size:
4969 3807 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4334 5121 6970 7835 8133 3562
Size: 772 787 1849 865 298 4274
Fragments arranged by size:
4274 1849 865 787 772 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 5222 7508 882
Size: 617 924 2799 2286 2219
Fragments arranged by size:
2799 2286 2219 924 617
BsaAI yAC'GTr
Cuts at: 493 1204 6636 493
Size: 711 5432 2702
Fragments arranged by size:
5432 2702 711
BsaBI GATnn'nnATC
Cuts at: 8839 8839
Size: 8845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 4389 4501 6652
Size: 53 83 186 936 612 2244 112 2151
Cuts at: 6652 7034 7669 275
Size: 382 635 1451
Fragments arranged by size:
2244 2151 1451 936 635 612 382 186
112 83 53
BsaWI w'CCGG_w
Cuts at: 5530 5877 7278 7676 7950 8097 8508 5530
Size: 347 1401 398 274 147 411 5867
Fragments arranged by size:
5867 1401 411 398 347 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 7810
Fragments arranged by size:
7810 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 4759 4762 5469 1186
Size: 1018 2555 3 707 4562
Fragments arranged by size:
4562 2555 1018 707 3
BseYI C'CCAG_C
Cuts at: 2077 2543 5059 8048 8544 2077
Size: 466 2516 2989 496 2378
Fragments arranged by size:
2989 2516 2378 496 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5559 1163
Size: 573 3823 4449
Fragments arranged by size:
4449 3823 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2030 2157 2173 2223 2263
Size: 275 186 16 13 127 16 50 40
Cuts at: 2263 2282 2389 2478 2626 2644 6295 7056 8084
Size: 19 107 89 148 18 3651 761 1028
Cuts at: 8084 1540
Size: 2301
Fragments arranged by size:
3651 2301 1028 761 275 186 148 127
107 89 50 40 19 18 16 16
13
BsiHKAI G_wGCw'C
Cuts at: 2087 4892 5137 6906 6991 8062 2087
Size: 2805 245 1769 85 1071 2870
Fragments arranged by size:
2870 2805 1769 1071 245 85
BsmI GAATG_Cn'
Cuts at: 3205 4245 6356 6449 3205
Size: 1040 2111 93 5601
Fragments arranged by size:
5601 2111 1040 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4508 4508
Size: 8845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2361 2575 2712 4892 5137 6164
Size: 399 297 274 214 137 2180 245 1027
Cuts at: 6164 6906 6991 8062 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2180 2174 1071 1027 742 399 297 274
245 214 137 85
BspEI T'CCGG_A
Cuts at: 5530 8508 5530
Size: 2978 5867
BspHI T'CATG_A
Cuts at: 5833 6025 6734 8464 5833
Size: 192 709 1730 6214
Fragments arranged by size:
6214 1730 709 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 3085 4020 6200 844
Size: 1700 541 935 2180 3489
Fragments arranged by size:
3489 2180 1700 935 541
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2026 2265 6732 1394
Size: 429 190 13 239 4467 3507
Fragments arranged by size:
4467 3507 429 239 190 13
BsrDI GCAATG_nn'
Cuts at: 65 1248 7340 7514 65
Size: 1183 6092 174 1396
Fragments arranged by size:
6092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2277 2306 2388 2477 2681 6062 7489
Size: 110 467 29 82 89 204 3381 1427
Cuts at: 7489 7676 1700
Size: 187 2869
Fragments arranged by size:
3381 2869 1427 467 204 187 110 89
82 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8845
BssHII G'CGCG_C
Cuts at: 1925 2719 1925
Size: 794 8051
BssSI C'ACGA_G
Cuts at: 5178 6905 7917 5178
Size: 1727 1012 6106
Fragments arranged by size:
6106 1727 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8845
BstBI TT'CG_AA
Cuts at: 7649 7649
Size: 8845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8845
BstXI CCAn_nnnn'nTGG
Cuts at: 3214 3367 4335 5407 3214
Size: 153 968 1072 6652
Fragments arranged by size:
6652 1072 968 153
BstYI r'GATC_y
Cuts at: 1972 2736 3185 3940 5609 6175 6542 6927 6944
Size: 764 449 755 1669 566 367 385 17
Cuts at: 6944 8396 8482 8494 8840 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1669 1452 764 755 566 449 385
367 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 5545 5545
Size: 8845
Bsu36I CC'TnA_GG
Cuts at: 2328 2328
Size: 8845
BtgI C'CryG_G
Cuts at: 513 1065 2004 2223 2488 4984 513
Size: 552 939 219 265 2496 4374
Fragments arranged by size:
4374 2496 939 552 265 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5773 487
Size: 683 56 4547 3559
Fragments arranged by size:
4547 3559 683 56
BtsI GCAGTG_nn'
Cuts at: 6361 7155 7175 6361
Size: 794 20 8031
Fragments arranged by size:
8031 794 20
ClaI AT'CG_AT
Cuts at: 1554 4798 1554
Size: 3244 5601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8810
DraI TTT'AAA
Cuts at: 2869 6149 2869
Size: 3280 5565
DraIII CAC_nnn'GTG
Cuts at: 4471 4471
Size: 8845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3773 7852 809
Size: 507 2457 4079 1802
Fragments arranged by size:
4079 2457 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2027 2154
Size: 54 1425 50 275 18 184 13 127
Cuts at: 2154 2220 2260 2279 2386 2475 2623 2641 5521
Size: 66 40 19 107 89 148 18 2880
Cuts at: 5521 6292 7181 8810 8
Size: 771 889 1629 43
Fragments arranged by size:
2880 1629 1425 889 771 275 184 148
127 107 89 66 54 50 43 40
19 18 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2014 2027 2154 2220 2260 2279 2386
Size: 275 202 13 127 66 40 19 107
Cuts at: 2386 2475 2623 2641 6292 1537
Size: 89 148 18 3651 4090
Fragments arranged by size:
4090 3651 275 202 148 127 107 89
66 40 19 18 13
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 3709 5619 6111 6775 1353
Size: 828 1528 1910 492 664 3423
Fragments arranged by size:
3423 1910 1528 828 664 492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 2498 7432 7806 7952 387
Size: 1064 138 540 369 4934 374 146 1280
Fragments arranged by size:
4934 1280 1064 540 374 369 146 138
EcoICRI GAG'CTC
Cuts at: 2085 5135 2085
Size: 3050 5795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2799 3381 3792 4509 4971 5381 6135
Size: 393 1167 582 411 717 462 410 754
Cuts at: 6135 6908 7505 8292 8560 1239
Size: 773 597 787 268 1524
Fragments arranged by size:
1524 1167 787 773 754 717 597 582
462 411 410 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 8845
EcoO109I rG'GnC_Cy
Cuts at: 2342 2494 2342
Size: 152 8693
EcoRI G'AATT_C
Cuts at: 3801 6154 6532 3801
Size: 2353 378 6114
Fragments arranged by size:
6114 2353 378
EcoRV GAT'ATC
Cuts at: 3633 3633
Size: 8845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2817 6019 2817
Size: 3202 5643
FseI GG_CCGG'CC
Cuts at: 2281 2392 2281
Size: 111 8734
FspI TGC'GCA
Cuts at: 7351 7351
Size: 8845
HaeII r_GCGC'y
Cuts at: 1678 2148 2207 4593 5765 7992 1678
Size: 470 59 2386 1172 2227 2531
Fragments arranged by size:
2531 2386 2227 1172 470 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2420 2452 3410
Size: 32 135 32 611 32 205 32 958
Cuts at: 3410 3442 3925 3957 4344 4376 7486 7518 7560
Size: 32 483 32 387 32 3110 32 42
Cuts at: 7560 7592 1373
Size: 32 2626
Fragments arranged by size:
3110 2626 958 611 483 387 205 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4036 4603 5080 6189 6435 669
Size: 988 281 2098 567 477 1109 246 3079
Fragments arranged by size:
3079 2098 1109 988 567 477 281 246
HindIII A'AGCT_T
Cuts at: 748 2659 2883 748
Size: 1911 224 6710
Fragments arranged by size:
6710 1911 224
HpaI GTT'AAC
Cuts at: 4036 6435 4036
Size: 2399 6446
KasI G'GCGC_C
Cuts at: 2144 2144
Size: 8845
KpnI G_GTAC'C
Cuts at: 5803 6170 5803
Size: 367 8478
MfeI C'AATT_G
Cuts at: 5072 6444 5072
Size: 1372 7473
MluI A'CGCG_T
Cuts at: 8721 8721
Size: 8845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2175 3691 4129 4718 4981 7959 8143 1305
Size: 870 1516 438 589 263 2978 184 2007
Fragments arranged by size:
2978 2007 1516 870 589 438 263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5523 10
Size: 54 1425 343 3691 3332
Fragments arranged by size:
3691 3332 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2985 3152 3212 3365 3699 4989
Size: 648 1362 457 167 60 153 334 1290
Cuts at: 4989 5405 5835 5997 6027 6803 7162 7321 518
Size: 416 430 162 30 776 359 159 2042
Fragments arranged by size:
2042 1362 1290 776 648 457 430 416
359 334 167 162 159 153 60 30
MspA1I CmG'CkG
Cuts at: 1606 2225 2284 2377 2490 2622 4736 5063 6261
Size: 619 59 93 113 132 2114 327 1198
Cuts at: 6261 6938 8086 8331 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 2114 1198 1148 677 619 327 245
132 113 93 59
NaeI GCC'GGC
Cuts at: 2279 2308 2390 2479 2279
Size: 29 82 89 8645
Fragments arranged by size:
8645 89 82 29
NarI GG'CG_CC
Cuts at: 2145 2145
Size: 8845
NcoI C'CATG_G
Cuts at: 513 1065 2004 4984 513
Size: 552 939 2980 4374
Fragments arranged by size:
4374 2980 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8845
NgoMIV G'CCGG_C
Cuts at: 2277 2306 2388 2477 2277
Size: 29 82 89 8645
Fragments arranged by size:
8645 89 82 29
NheI G'CTAG_C
Cuts at: 1052 3434 4664 1052
Size: 2382 1230 5233
Fragments arranged by size:
5233 2382 1230
NotI GC'GGCC_GC
Cuts at: 2027 2220 2623 2641 6292 2027
Size: 193 403 18 3651 4580
Fragments arranged by size:
4580 3651 403 193 18
NsiI A_TGCA'T
Cuts at: 2421 3696 2421
Size: 1275 7570
NspI r_CATG'y
Cuts at: 1132 1229 2419 6146 6203 7748 1132
Size: 97 1190 3727 57 1545 2229
Fragments arranged by size:
3727 2229 1545 1190 97 57
PacI TTA_AT'TAA
Cuts at: 4722 4722
Size: 8845
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8845
PciI A'CATG_T
Cuts at: 6142 7744 6142
Size: 1602 7243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8845
PfoI T'CCnGG_A
Cuts at: 2932 3277 4513 5449 6096 2932
Size: 345 1236 936 647 5681
Fragments arranged by size:
5681 1236 936 647 345
PmeI GTTT'AAAC
Cuts at: 6149 6149
Size: 8845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2150 2182 4386 4418 6914 6946 8427 8459 2150
Size: 32 2204 32 2496 32 1481 32 2536
Fragments arranged by size:
2536 2496 2204 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 2494
Size: 8845
PshAI GACnn'nnGTC
Cuts at: 1613 2634 5271 1613
Size: 1021 2637 5187
Fragments arranged by size:
5187 2637 1021
PsiI TTA'TAA
Cuts at: 4524 6415 4524
Size: 1891 6954
PspOMI G'GGCC_C
Cuts at: 2357 2357
Size: 8845
PspXI vC'TCGA_Gb
Cuts at: 4542 4542
Size: 8845
PstI C_TGCA'G
Cuts at: 830 6197 830
Size: 5367 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8845
PvuII CAG'CTG
Cuts at: 5063 5063
Size: 8845
SacI G_AGCT'C
Cuts at: 2087 5137 2087
Size: 3050 5795
SacII CC_GC'GG
Cuts at: 2226 2491 2226
Size: 265 8580
SalI G'TCGA_C
Cuts at: 1936 6187 1936
Size: 4251 4594
SanDI GG'GwC_CC
Cuts at: 2494 2494
Size: 8845
SbfI CC_TGCA'GG
Cuts at: 6197 6197
Size: 8845
ScaI AGT'ACT
Cuts at: 7093 8805 7093
Size: 1712 7133
SexAI A'CCwGG_T
Cuts at: 3658 3658
Size: 8845
SfcI C'TryA_G
Cuts at: 826 1045 2839 6193 7328 8009 8200 826
Size: 219 1794 3354 1135 681 191 1471
Fragments arranged by size:
3354 1794 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2235 2235
Size: 8845
SfoI GGC'GCC
Cuts at: 2146 2146
Size: 8845
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8845
SgrAI Cr'CCGG_yG
Cuts at: 1700 2681 1700
Size: 981 7864
SmaI CCC'GGG
Cuts at: 1872 2093 6172 1872
Size: 221 4079 4545
Fragments arranged by size:
4545 4079 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4313 4542 5136 6949 7850
Size: 197 926 1598 772 229 594 1813 901
Cuts at: 7850 8112 820
Size: 262 1553
Fragments arranged by size:
1813 1598 1553 926 901 772 594 262
229 197
SnaBI TAC'GTA
Cuts at: 493 6636 493
Size: 6143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8845
SphI G_CATG'C
Cuts at: 1132 6203 1132
Size: 5071 3774
SrfI GCCC'GGGC
Cuts at: 2093 2093
Size: 8845
SspI AAT'ATT
Cuts at: 5 52 3457 4187 5446 6769 5
Size: 47 3405 730 1259 1323 2081
Fragments arranged by size:
3405 2081 1323 1259 730 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2033 3588 4128 4984 5894 6284
Size: 552 939 29 1555 540 856 910 390
Cuts at: 6284 513
Size: 3074
Fragments arranged by size:
3074 1555 939 910 856 552 540 390
29
SwaI ATTT'AAAT
Cuts at: 2869 2869
Size: 8845
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 4482 6872 6889 7042 7227 7672
Size: 1255 698 2204 2390 17 153 185 445
Cuts at: 7672 325
Size: 1498
Fragments arranged by size:
2390 2204 1498 1255 698 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3678 3997 5150
Size: 274 80 33 51 1443 1701 319 1153
Cuts at: 5150 5332 5701 5752 7091 8803 96
Size: 182 369 51 1339 1712 138
Fragments arranged by size:
1712 1701 1443 1339 1153 369 319 274
182 138 80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3690 3722 4895 4927 8805 8837 3690
Size: 32 1173 32 3878 32 3698
Fragments arranged by size:
3878 3698 1173 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 7727
XbaI T'CTAG_A
Cuts at: 4405 6181 4405
Size: 1776 7069
XhoI C'TCGA_G
Cuts at: 1943 4542 1943
Size: 2599 6246
XmaI C'CCGG_G
Cuts at: 1870 2091 6170 1870
Size: 221 4079 4545
Fragments arranged by size:
4545 4079 221
XmnI GAAnn'nnTTC
Cuts at: 3141 4188 6974 3141
Size: 1047 2786 5012
Fragments arranged by size:
5012 2786 1047
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6653 276
Size: 53 83 186 936 5119 2468
Fragments arranged by size:
5119 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BplI BsiWI FspAI
NruI PmlI PsrI RsrII SapI XcmI