Restriction map for
pFN21AB5535
(Circular) (Six-base) MAPSORT of: pFN21AB5535.seq Check: 9194 from: 1 to: 6094
pFN21AB5535 6094 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2092 2092
Size: 6094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2821 3437 1937
Size: 884 616 4594
Fragments arranged by size:
4594 884 616
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 4157 5541 5809 1632
Size: 560 1965 1384 268 1917
Fragments arranged by size:
1965 1917 1384 560 268
AflII C'TTAA_G
Cuts at: 820 1017 2471 820
Size: 197 1454 4443
Fragments arranged by size:
4443 1454 197
AflIII A'CryG_T
Cuts at: 1203 4993 5970 1203
Size: 3790 977 1327
Fragments arranged by size:
3790 1327 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 2215 2984 3257 3837 5409 2215
Size: 769 273 580 1572 2900
Fragments arranged by size:
2900 1572 769 580 273
ApaI G_GGCC'C
Cuts at: 2019 3111 2019
Size: 1092 5002
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 2476 3403 3629 3781 5890 1493
Size: 983 927 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 927 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3419 1870
Size: 73 1476 4545
Fragments arranged by size:
4545 1476 73
BamHI G'GATC_C
Cuts at: 3324 3424 3791 3324
Size: 100 367 5627
Fragments arranged by size:
5627 367 100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2114 3293 3415 4870
Size: 325 217 627 276 51 1179 122 1455
Cuts at: 4870 618
Size: 1842
Fragments arranged by size:
1842 1455 1179 627 325 276 217 122
51
BanII G_rGCy'C
Cuts at: 1391 2019 2138 3111 3413 1391
Size: 628 119 973 302 4072
Fragments arranged by size:
4072 973 628 302 119
BbeI G_GCGC'C
Cuts at: 2067 2067
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2116 3015 928
Size: 971 217 899 4007
Fragments arranged by size:
4007 971 899 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2066 2100 4285 4319 1903
Size: 34 129 34 2185 34 3678
Fragments arranged by size:
3678 2185 129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2212 3986 5196 2212
Size: 1774 1210 3110
Fragments arranged by size:
3110 1774 1210
BclI T'GATC_A
Cuts at: 1548 2515 1548
Size: 967 5127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4705 136
Size: 107 122 71 631 605 3033 1525
Fragments arranged by size:
3033 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2117 3111 3296 4155 5311 1790
Size: 229 98 994 185 859 1156 2573
Fragments arranged by size:
2573 1156 994 859 229 185 98
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2382 2413 3825
Size: 416 53 83 399 24 965 31 1412
Cuts at: 3825 4783 442
Size: 958 1753
Fragments arranged by size:
1753 1412 965 958 416 399 83 53
31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2734 4754 1239
Size: 883 612 2020 2579
Fragments arranged by size:
2579 2020 883 612
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 6094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2874 3110 4219 5084 5382 2874
Size: 236 1109 865 298 3586
Fragments arranged by size:
3586 1109 865 298 236
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3180 4757 882
Size: 617 1681 1577 2219
Fragments arranged by size:
2219 1681 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3901 4283 4918
Size: 53 83 186 936 531 1837 382 635
Cuts at: 4918 275
Size: 1451
Fragments arranged by size:
1837 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2417 3321 4527 4925 5199 5346 5757 2417
Size: 904 1206 398 274 147 411 2754
Fragments arranged by size:
2754 1206 904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2605 2635 2855 2885 2974
Size: 30 165 30 1202 30 220 30 89
Cuts at: 2974 3004 1178
Size: 30 4268
Fragments arranged by size:
4268 1202 220 165 89 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3019 1186
Size: 1833 4261
BseYI C'CCAG_C
Cuts at: 2012 2110 2148 3288 3300 5297 5793 2012
Size: 98 38 1140 12 1997 496 2313
Fragments arranged by size:
2313 1997 1140 496 98 38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 2640 1163
Size: 573 341 563 4617
Fragments arranged by size:
4617 573 563 341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3544 4305 5333 1540
Size: 275 186 1543 761 1028 2301
Fragments arranged by size:
2301 1543 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4155 4240 5311 4155
Size: 85 1071 4938
Fragments arranged by size:
4938 1071 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2117 2138 3111 3296 3413 4155
Size: 399 229 98 21 973 185 117 742
Cuts at: 4155 4240 5311 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 973 742 399 229 185 117
98 85 21
BspEI T'CCGG_A
Cuts at: 3321 5757 3321
Size: 2436 3658
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2092 2115 2662 3449 844
Size: 1248 23 547 787 3489
Fragments arranged by size:
3489 1248 787 547 23
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3076 4589 4763 65
Size: 1183 1828 1513 174 1396
Fragments arranged by size:
1828 1513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3296 4738 4925 1700
Size: 110 1486 1442 187 2869
Fragments arranged by size:
2869 1486 1442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 2752 4898 2752
Size: 2146 3948
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 4934
BstXI CCAn_nnnn'nTGG
Cuts at: 2706 2945 3176 2706
Size: 239 231 5624
Fragments arranged by size:
5624 239 231
BstYI r'GATC_y
Cuts at: 1972 3162 3324 3424 3791 4176 4193 5645 5731
Size: 1190 162 100 367 385 17 1452 86
Cuts at: 5731 5743 6089 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1190 385 367 346 162 100
86 17 12
BstZ17I GTA'TAC
Cuts at: 2822 2822
Size: 6094
Bsu36I CC'TnA_GG
Cuts at: 2859 2859
Size: 6094
BtgI C'CryG_G
Cuts at: 513 1065 2004 3111 513
Size: 552 939 1107 3496
Fragments arranged by size:
3496 1107 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2169 487
Size: 683 56 943 4412
Fragments arranged by size:
4412 943 683 56
BtsI GCAGTG_nn'
Cuts at: 3372 3610 4404 4424 3372
Size: 238 794 20 5042
Fragments arranged by size:
5042 794 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2623 3541
Size: 54 1425 50 275 18 418 375 918
Cuts at: 3541 4430 6059 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 918 889 418 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 4024 1353
Size: 972 1699 3423
Fragments arranged by size:
3423 1699 972
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2192 2734 4157 4754 5541 5809
Size: 393 490 70 542 1423 597 787 268
Cuts at: 5809 1239
Size: 1524
Fragments arranged by size:
1524 1423 787 597 542 490 393 268
70
EcoO109I rG'GnC_Cy
Cuts at: 2016 2016
Size: 6094
EcoRI G'AATT_C
Cuts at: 2476 3403 3781 2476
Size: 927 378 4789
Fragments arranged by size:
4789 927 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3207 3207
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2067 5241 1678
Size: 389 3174 2531
Fragments arranged by size:
3174 2531 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 3239 3271 4735
Size: 32 135 32 1283 32 352 32 1464
Cuts at: 4735 4767 4809 4841 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 1283 352 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 2458 3438 3684 669
Size: 988 281 223 297 980 246 3079
Fragments arranged by size:
3079 988 980 297 281 246 223
HindIII A'AGCT_T
Cuts at: 748 2961 3086 748
Size: 2213 125 3756
Fragments arranged by size:
3756 2213 125
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 2063 2063
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3351 3693 3351
Size: 342 5752
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 2625 10
Size: 54 1425 343 418 375 3479
Fragments arranged by size:
3479 1425 418 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 4052 4411 4570 518
Size: 648 2199 687 359 159 2042
Fragments arranged by size:
2199 2042 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2775 3288 3510 4187 5335 5580 1606
Size: 536 633 513 222 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 536 513 245 222
NarI GG'CG_CC
Cuts at: 2064 2064
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 3111 513
Size: 552 939 1107 3496
Fragments arranged by size:
3496 1107 939 552
NdeI CA'TA_TG
Cuts at: 387 2227 387
Size: 1840 4254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 3452 4997 1132
Size: 97 2223 1545 2229
Fragments arranged by size:
2229 2223 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 4846
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 2015 3107 2015
Size: 1092 5002
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2775 3288 2775
Size: 513 5581
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 3442 4577 5258 5449 826
Size: 219 2397 1135 681 191 1471
Fragments arranged by size:
2397 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2065 2065
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 2889 3089 4198 5099 5361
Size: 197 926 528 418 200 1109 901 262
Cuts at: 5361 820
Size: 1553
Fragments arranged by size:
1553 1109 926 901 528 418 262 200
197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2236 2272 2642 3111 3533 513
Size: 552 939 232 36 370 469 422 3074
Fragments arranged by size:
3074 939 552 469 422 370 232 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2364 2368 4121 4138 4291 4476
Size: 1255 417 367 4 1753 17 153 185
Cuts at: 4476 4921 325
Size: 445 1498
Fragments arranged by size:
1753 1498 1255 445 417 367 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4340 6052 96
Size: 274 80 33 51 1443 2363 1712 138
Fragments arranged by size:
2363 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2412 2444 2605 2637 2974 3006 3367 3399 6054
Size: 32 161 32 337 32 361 32 2655
Cuts at: 6054 6086 2412
Size: 32 2420
Fragments arranged by size:
2655 2420 361 337 161 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2765 2765
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI