Restriction map for
pFN21AB5530
(Circular) (Six-base) MAPSORT of: pFN21AB5530.seq Check: 8634 from: 1 to: 5065
pFN21AB5530 5065 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2875 278
Size: 53 83 186 936 1339 2468
Fragments arranged by size:
2468 1339 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2408 1937
Size: 471 4594
Acc65I G'GTAC_C
Cuts at: 2386 2386
Size: 5065
AclI AA'CG_TT
Cuts at: 1563 3192 3565 1563
Size: 1629 373 3063
Fragments arranged by size:
3063 1629 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3128 4512 4780 1632
Size: 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4868
AflIII A'CryG_T
Cuts at: 1203 3964 4941 1203
Size: 2761 977 1327
Fragments arranged by size:
2761 1327 977
AgeI A'CCGG_T
Cuts at: 3896 3896
Size: 5065
AhdI GACnn_n'nnGTC
Cuts at: 3794 3794
Size: 5065
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5065
AlwNI CAG_nnn'CTG
Cuts at: 2808 4380 2808
Size: 1572 3493
ApaLI G'TGCA_C
Cuts at: 3122 4278 3122
Size: 1156 3909
ApoI r'AATT_y
Cuts at: 1493 2374 2600 2752 4861 1493
Size: 881 226 152 2109 1697
Fragments arranged by size:
2109 1697 881 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3619 160
Size: 872 2587 1606
Fragments arranged by size:
2587 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5065
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2390 1870
Size: 73 174 273 4545
Fragments arranged by size:
4545 273 174 73
BamHI G'GATC_C
Cuts at: 2395 2762 2395
Size: 367 4698
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2386 3841 618
Size: 325 217 627 471 128 1455 1842
Fragments arranged by size:
1842 1455 627 471 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2384 1391
Size: 993 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 928
Size: 971 378 3716
Fragments arranged by size:
3716 971 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3256 3290 1903
Size: 34 1319 34 3678
Fragments arranged by size:
3678 1319 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2957 4167 2957
Size: 1210 3855
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3676 136
Size: 107 122 71 631 605 2004 1525
Fragments arranged by size:
2004 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5060 5060
Size: 5065
BlpI GC'TnA_GC
Cuts at: 2482 2482
Size: 5065
Bme1580I G_kGCm'C
Cuts at: 1790 3126 4282 1790
Size: 1336 1156 2573
Fragments arranged by size:
2573 1336 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2796 3754 442
Size: 416 53 83 399 24 1379 958 1753
Fragments arranged by size:
1753 1379 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3725 1239
Size: 2486 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3190 4055 4353 3190
Size: 865 298 3902
Fragments arranged by size:
3902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3728 882
Size: 617 2229 2219
Fragments arranged by size:
2229 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2856 493
Size: 711 1652 2702
Fragments arranged by size:
2702 1652 711
BsaBI GATnn'nnATC
Cuts at: 2360 5059 2360
Size: 2699 2366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2872 3254 3889
Size: 53 83 186 936 549 790 382 635
Cuts at: 3889 275
Size: 1451
Fragments arranged by size:
1451 936 790 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3498 3896 4170 4317 4728 3498
Size: 398 274 147 411 3835
Fragments arranged by size:
3835 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4840
Fragments arranged by size:
4840 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5065
BseYI C'CCAG_C
Cuts at: 2036 4268 4764 2036
Size: 2232 496 2337
Fragments arranged by size:
2337 2232 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2515 3276 4304 1540
Size: 275 186 514 761 1028 2301
Fragments arranged by size:
2301 1028 761 514 275 186
BsiHKAI G_wGCw'C
Cuts at: 3126 3211 4282 3126
Size: 85 1071 3909
Fragments arranged by size:
3909 1071 85
BsmI GAATG_Cn'
Cuts at: 2576 2669 2576
Size: 93 4972
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2384 3126 3211 4282 1391
Size: 399 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 399 85
BspEI T'CCGG_A
Cuts at: 4728 4728
Size: 5065
BspHI T'CATG_A
Cuts at: 2098 2954 4684 2098
Size: 856 1730 2479
Fragments arranged by size:
2479 1730 856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 844
Size: 1576 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2952 1394
Size: 429 1129 3507
Fragments arranged by size:
3507 1129 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3560 3734 65
Size: 1183 2312 174 1396
Fragments arranged by size:
2312 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 3709 3896 1700
Size: 110 208 1691 187 2869
Fragments arranged by size:
2869 1691 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5065
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5065
BssSI C'ACGA_G
Cuts at: 3125 4137 3125
Size: 1012 4053
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5065
BstBI TT'CG_AA
Cuts at: 3869 3869
Size: 5065
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5065
BstYI r'GATC_y
Cuts at: 1972 2395 2762 3147 3164 4616 4702 4714 5060
Size: 423 367 385 17 1452 86 12 346
Cuts at: 5060 1972
Size: 1977
Fragments arranged by size:
1977 1452 423 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2202 513
Size: 552 1002 135 3376
Fragments arranged by size:
3376 1002 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4326
Fragments arranged by size:
4326 683 56
BtsI GCAGTG_nn'
Cuts at: 2581 3375 3395 2581
Size: 794 20 4251
Fragments arranged by size:
4251 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5030
DraI TTT'AAA
Cuts at: 2369 2369
Size: 5065
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4072 809
Size: 507 2756 1802
Fragments arranged by size:
2756 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2070 2512
Size: 54 1425 50 275 18 196 44 442
Cuts at: 2512 3401 5030 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 442 275 196 54 50
44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2512 1537
Size: 275 700 4090
Fragments arranged by size:
4090 700 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 2995 1353
Size: 782 860 3423
Fragments arranged by size:
3423 860 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3652 4026 4172 387
Size: 1064 138 2063 374 146 1280
Fragments arranged by size:
2063 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3128 3725 4512 4780 1239
Size: 393 1496 597 787 268 1524
Fragments arranged by size:
1524 1496 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2374 2752 2374
Size: 378 4687
FspI TGC'GCA
Cuts at: 3571 3571
Size: 5065
HaeII r_GCGC'y
Cuts at: 1678 4212 1678
Size: 2534 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3706 3738 3780 3812 1373
Size: 32 135 32 2134 32 42 32 2626
Fragments arranged by size:
2626 2134 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2409 2655 669
Size: 988 281 471 246 3079
Fragments arranged by size:
3079 988 471 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5065
HpaI GTT'AAC
Cuts at: 2655 2655
Size: 5065
KpnI G_GTAC'C
Cuts at: 2390 2390
Size: 5065
MfeI C'AATT_G
Cuts at: 2664 2664
Size: 5065
MluI A'CGCG_T
Cuts at: 4941 4941
Size: 5065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4179 4363 1305
Size: 858 2016 184 2007
Fragments arranged by size:
2016 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 3003
Fragments arranged by size:
3003 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3023 3382 3541 518
Size: 648 1857 359 159 2042
Fragments arranged by size:
2042 1857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2481 3158 4306 4551 1606
Size: 419 456 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 456 419 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 5065
NcoI C'CATG_G
Cuts at: 513 1065 2202 513
Size: 552 1137 3376
Fragments arranged by size:
3376 1137 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5065
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 5065
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5065
NotI GC'GGCC_GC
Cuts at: 2512 2512
Size: 5065
NspI r_CATG'y
Cuts at: 1132 1229 2330 2423 3968 1132
Size: 97 1101 93 1545 2229
Fragments arranged by size:
2229 1545 1101 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5065
PciI A'CATG_T
Cuts at: 3964 3964
Size: 5065
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5065
PmeI GTTT'AAAC
Cuts at: 2369 2369
Size: 5065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 4647 4679 3134
Size: 32 1481 32 3520
Fragments arranged by size:
3520 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5065
PsiI TTA'TAA
Cuts at: 2635 2635
Size: 5065
PstI C_TGCA'G
Cuts at: 830 2417 830
Size: 1587 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5065
SalI G'TCGA_C
Cuts at: 1936 2407 1936
Size: 471 4594
SapI GCTCTTCn'nnn_
Cuts at: 2135 2135
Size: 5065
SbfI CC_TGCA'GG
Cuts at: 2417 2417
Size: 5065
ScaI AGT'ACT
Cuts at: 3313 5025 3313
Size: 1712 3353
SfcI C'TryA_G
Cuts at: 826 1045 2413 3548 4229 4420 826
Size: 219 1368 1135 681 191 1471
Fragments arranged by size:
1471 1368 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5065
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5065
SmaI CCC'GGG
Cuts at: 1872 2119 2392 1872
Size: 247 273 4545
Fragments arranged by size:
4545 273 247
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3169 4070 4332 820
Size: 197 926 1226 901 262 1553
Fragments arranged by size:
1553 1226 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2856 493
Size: 2363 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5065
SphI G_CATG'C
Cuts at: 1132 2423 1132
Size: 1291 3774
SspI AAT'ATT
Cuts at: 5 52 2989 5
Size: 47 2937 2081
Fragments arranged by size:
2937 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5065
StyI C'CwwG_G
Cuts at: 513 1065 2202 2504 513
Size: 552 1137 302 3074
Fragments arranged by size:
3074 1137 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3092 3109 3262 3447 3892 325
Size: 1255 1512 17 153 185 445 1498
Fragments arranged by size:
1512 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3311 5023 96
Size: 274 80 33 51 1443 1334 1712 138
Fragments arranged by size:
1712 1443 1334 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5025 5057 5025
Size: 32 5033
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5065
XbaI T'CTAG_A
Cuts at: 2401 2401
Size: 5065
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2065
Size: 5065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5065
XmaI C'CCGG_G
Cuts at: 1870 2117 2390 1870
Size: 247 273 4545
Fragments arranged by size:
4545 273 247
XmnI GAAnn'nnTTC
Cuts at: 3194 3194
Size: 5065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2873 276
Size: 53 83 186 936 1339 2468
Fragments arranged by size:
2468 1339 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI