Restriction map for
pFN21AB5529
(Circular) (Six-base) MAPSORT of: pFN21AB5529.seq Check: 4132 from: 1 to: 5605
pFN21AB5529 5605 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2821 2821
Size: 5605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2926 2926
Size: 5605
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3668 5052 5320 1632
Size: 725 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 725 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 4504 5481 1203
Size: 3301 977 1327
Fragments arranged by size:
3301 1327 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 2131 4334 2131
Size: 2203 3402
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2244 2342 2657 2822 1184
Size: 1060 98 315 165 3967
Fragments arranged by size:
3967 1060 315 165 98
AlwNI CAG_nnn'CTG
Cuts at: 2389 2392 3348 4920 2389
Size: 3 956 1572 3074
Fragments arranged by size:
3074 1572 956 3
ApaI G_GGCC'C
Cuts at: 2188 2313 2605 2188
Size: 125 292 5188
Fragments arranged by size:
5188 292 125
ApaLI G'TGCA_C
Cuts at: 2327 3662 4818 2327
Size: 1335 1156 3114
Fragments arranged by size:
3114 1335 1156
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2164 2306 2930 1870
Size: 73 110 111 142 624 4545
Fragments arranged by size:
4545 624 142 111 110 73
AvrII C'CTAG_G
Cuts at: 2758 2758
Size: 5605
BamHI G'GATC_C
Cuts at: 2407 2935 3302 2407
Size: 528 367 4710
Fragments arranged by size:
4710 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2926 4381 618
Size: 325 217 627 293 846 1455 1842
Fragments arranged by size:
1842 1455 846 627 325 293 217
BanII G_rGCy'C
Cuts at: 1391 2060 2188 2313 2605 2924 1391
Size: 669 128 125 292 319 4072
Fragments arranged by size:
4072 669 319 292 128 125
BbeI G_GCGC'C
Cuts at: 2084 2084
Size: 5605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BbvCI CC'TCA_GC
Cuts at: 2103 2276 2738 2103
Size: 173 462 4970
Fragments arranged by size:
4970 462 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2166 2299 4216
Size: 107 122 71 631 605 494 133 1917
Cuts at: 4216 136
Size: 1525
Fragments arranged by size:
1917 1525 631 605 494 133 122 107
71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2188 2313 2331 2605 3666 4822 1790
Size: 398 125 18 274 1061 1156 2573
Fragments arranged by size:
2573 1156 1061 398 274 125 18
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 4711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 2683 3336
Size: 416 53 83 399 24 765 501 653
Cuts at: 3336 4294 442
Size: 958 1753
Fragments arranged by size:
1753 958 765 653 501 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 2771 4265 1239
Size: 1493 39 1494 2579
Fragments arranged by size:
2579 1494 1493 39
Bpu10I CC'TnA_GC
Cuts at: 2044 2103 2276 2738 2044
Size: 59 173 462 4911
Fragments arranged by size:
4911 462 173 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2174 3412 3794
Size: 53 83 186 936 548 93 1238 382
Cuts at: 3794 4429 275
Size: 635 1451
Fragments arranged by size:
1451 1238 936 635 548 382 186 93
83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 4699
Fragments arranged by size:
4699 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2610 1186
Size: 1424 4181
BseYI C'CCAG_C
Cuts at: 2140 2527 4808 5304 2140
Size: 387 2281 496 2441
Fragments arranged by size:
2441 2281 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2436 2501 2528 2542 2627 2782
Size: 573 666 34 65 27 14 85 155
Cuts at: 2782 1163
Size: 3986
Fragments arranged by size:
3986 666 573 155 85 65 34 27
14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 2331 3666 3751 4822 2060
Size: 271 1335 85 1071 2843
Fragments arranged by size:
2843 1335 1071 271 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2188 2313 2331 2605 2924 3666
Size: 399 270 128 125 18 274 319 742
Cuts at: 3666 3751 4822 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 274 270 128
125 85 18
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2821 2960 844
Size: 1977 139 3489
Fragments arranged by size:
3489 1977 139
BsrBI CCG'CTC
Cuts at: 1394 1823 3492 1394
Size: 429 1669 3507
Fragments arranged by size:
3507 1669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 2473 4100 4274 65
Size: 1183 1219 6 1627 174 1396
Fragments arranged by size:
1627 1396 1219 1183 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 2298 4249 4436 1700
Size: 110 461 27 1951 187 2869
Fragments arranged by size:
2869 1951 461 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 2281 1925
Size: 356 5249
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 794
Size: 1595 4010
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2371 2522 2371
Size: 151 5454
BstYI r'GATC_y
Cuts at: 1972 2407 2935 3302 3687 3704 5156 5242 5254
Size: 435 528 367 385 17 1452 86 12
Cuts at: 5254 5600 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 528 435 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 2497 513
Size: 552 939 309 184 3621
Fragments arranged by size:
3621 939 552 309 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2540 2585 3121 3915 3935 2540
Size: 45 536 794 20 4210
Fragments arranged by size:
4210 794 536 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2269 2355 2649
Size: 54 1425 50 275 18 439 86 294
Cuts at: 2649 2856 3052 3941 5570 8
Size: 207 196 889 1629 43
Fragments arranged by size:
1629 1425 889 439 294 275 207 196
86 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2333 2894 3535 1353
Size: 764 216 561 641 3423
Fragments arranged by size:
3423 764 641 561 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2058 2058
Size: 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2732 2771 3668 4265 5052 5320
Size: 393 725 375 39 897 597 787 268
Cuts at: 5320 1239
Size: 1524
Fragments arranged by size:
1524 897 787 725 597 393 375 268
39
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 2675
Size: 5605
EcoO109I rG'GnC_Cy
Cuts at: 2107 2184 2292 2601 2107
Size: 77 108 309 5111
Fragments arranged by size:
5111 309 108 77
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2084 2758 4752 1678
Size: 406 674 1994 2531
Fragments arranged by size:
2531 1994 674 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2731 2763 4246
Size: 32 135 32 545 32 582 32 1483
Cuts at: 4246 4278 4320 4352 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1483 582 545 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KasI G'GCGC_C
Cuts at: 2080 2080
Size: 5605
KpnI G_GTAC'C
Cuts at: 2930 2930
Size: 5605
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 2858 10
Size: 54 1425 343 819 207 2757
Fragments arranged by size:
2757 1425 819 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 3563 3922 4081 518
Size: 648 1566 831 359 159 2042
Fragments arranged by size:
2042 1566 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2197 2267 2389 2420 2485 3021 3698
Size: 486 105 70 122 31 65 536 677
Cuts at: 3698 4846 5091 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 486 245 122 105
70 65 31
NaeI GCC'GGC
Cuts at: 2273 2273
Size: 5605
NarI GG'CG_CC
Cuts at: 2081 2081
Size: 5605
NcoI C'CATG_G
Cuts at: 513 1065 2004 2497 513
Size: 552 939 493 3621
Fragments arranged by size:
3621 939 552 493
NdeI CA'TA_TG
Cuts at: 387 2733 387
Size: 2346 3259
NgoMIV G'CCGG_C
Cuts at: 2271 2271
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5605
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspOMI G'GGCC_C
Cuts at: 2184 2309 2601 2184
Size: 125 292 5188
Fragments arranged by size:
5188 292 125
PspXI vC'TCGA_Gb
Cuts at: 2053 2053
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2676 2811 2883 2957 830
Size: 1846 135 72 74 3478
Fragments arranged by size:
3478 1846 135 74 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2389 2420 2485 2389
Size: 31 65 5509
Fragments arranged by size:
5509 65 31
SacI G_AGCT'C
Cuts at: 2060 2060
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SbfI CC_TGCA'GG
Cuts at: 2676 2957 2676
Size: 281 5324
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SfcI C'TryA_G
Cuts at: 826 1045 2672 2807 2879 2953 4088 4769 4960
Size: 219 1627 135 72 74 1135 681 191
Cuts at: 4960 826
Size: 1471
Fragments arranged by size:
1627 1471 1135 681 219 191 135 74
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2166 2166
Size: 5605
SfoI GGC'GCC
Cuts at: 2082 2082
Size: 5605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 2298 1700
Size: 598 5007
SmaI CCC'GGG
Cuts at: 1872 2932 1872
Size: 1060 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 3709 4610 4872 820
Size: 197 926 110 1656 901 262 1553
Fragments arranged by size:
1656 1553 926 901 262 197 110
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 2497 2758 3044 513
Size: 552 939 493 261 286 3074
Fragments arranged by size:
3074 939 552 493 286 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2637 3632 3649 3802 3987 4432 325
Size: 1255 1057 995 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1057 995 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 5565 5597 2054
Size: 32 3479 32 2062
Fragments arranged by size:
3479 2062 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2830 2830
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 2053 1943
Size: 110 5495
XmaI C'CCGG_G
Cuts at: 1870 2930 1870
Size: 1060 4545
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI
SexAI SrfI SwaI