Restriction map for
pFN21AB5521
(Circular) (Six-base) MAPSORT of: pFN21AB5521.seq Check: 4711 from: 1 to: 6049
pFN21AB5521 6049 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2743 2930 4112 5496 5764 1632
Size: 1111 187 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 1111 268 187
AfeI AGC'GCT
Cuts at: 2794 2794
Size: 6049
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 2137 4948 5925 1203
Size: 934 2811 977 1327
Fragments arranged by size:
2811 1327 977 934
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 6017
AlwNI CAG_nnn'CTG
Cuts at: 3792 5364 3792
Size: 1572 4477
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 2606 2899 2922 3358 3584 3736 5845 1493
Size: 1113 293 23 436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1113 436 293 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 1870
Size: 73 1431 4545
Fragments arranged by size:
4545 1431 73
AvrII C'CTAG_G
Cuts at: 2270 2270
Size: 6049
BamHI G'GATC_C
Cuts at: 2839 3379 3746 2839
Size: 540 367 5142
Fragments arranged by size:
5142 540 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3370 4825 618
Size: 325 217 627 1583 1455 1842
Fragments arranged by size:
1842 1583 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2513 2718 3348 3368 1391
Size: 1122 205 630 20 4072
Fragments arranged by size:
4072 1122 630 205 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 928
Size: 971 665 4413
Fragments arranged by size:
4413 971 665
BbvCI CC'TCA_GC
Cuts at: 2796 2796
Size: 6049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 4110 5266 1790
Size: 2320 1156 2573
Fragments arranged by size:
2573 2320 1156
BmgBI CAC'GTC
Cuts at: 1318 2769 1318
Size: 1451 4598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 4738 442
Size: 416 53 83 399 24 2363 958 1753
Fragments arranged by size:
2363 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 4709 1239
Size: 973 2497 2579
Fragments arranged by size:
2579 2497 973
Bpu10I CC'TnA_GC
Cuts at: 2796 2796
Size: 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2058 2402 2527 3343 4174 5039 5337 2058
Size: 344 125 816 831 865 298 2770
Fragments arranged by size:
2770 865 831 816 344 298 125
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2190 4712 882
Size: 617 691 2522 2219
Fragments arranged by size:
2522 2219 691 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3856 4238 4873 275
Size: 53 83 186 936 2323 382 635 1451
Fragments arranged by size:
2323 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5824
Fragments arranged by size:
5824 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 2813 1186
Size: 1540 87 4422
Fragments arranged by size:
4422 1540 87
BseYI C'CCAG_C
Cuts at: 2547 2789 3313 5252 5748 2547
Size: 242 524 1939 496 2848
Fragments arranged by size:
2848 1939 524 496 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3035 3499 4260 5288 1540
Size: 275 186 1034 464 761 1028 2301
Fragments arranged by size:
2301 1034 1028 761 464 275 186
BsiHKAI G_wGCw'C
Cuts at: 2718 4110 4195 5266 2718
Size: 1392 85 1071 3501
Fragments arranged by size:
3501 1392 1071 85
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2718 3348 3368 4110 4195 5266
Size: 399 723 205 630 20 742 85 1071
Cuts at: 5266 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 723 630 399 205 85
20
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 2116 3938 5668 2116
Size: 1822 1730 2497
Fragments arranged by size:
2497 1822 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2941 3936 1394
Size: 429 1118 995 3507
Fragments arranged by size:
3507 1118 995 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 3089 4544 4718 65
Size: 1183 969 872 1455 174 1396
Fragments arranged by size:
1455 1396 1183 969 872 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2668 4693 4880 1700
Size: 110 858 2025 187 2869
Fragments arranged by size:
2869 2025 858 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 2646 4109 5121 2646
Size: 1463 1012 3574
Fragments arranged by size:
3574 1463 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 2597 2597
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2386 2839 2958 3379 3746 4131 4148 5600
Size: 414 453 119 421 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 453 421 414 385 367 346
119 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2934 2934
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 2565 513
Size: 552 1500 3997
Fragments arranged by size:
3997 1500 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 2018 2323 2892 3565 4359 4379 2018
Size: 305 569 673 794 20 3688
Fragments arranged by size:
3688 794 673 569 305 20
ClaI AT'CG_AT
Cuts at: 1554 2459 1554
Size: 905 5144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 3496 4385
Size: 54 1425 50 275 18 706 960 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1425 960 889 706 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2056 2495 2703 2906 3979 1353
Size: 703 439 208 203 1073 3423
Fragments arranged by size:
3423 1073 703 439 208 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2428 4636 5010 5156 387
Size: 1064 138 839 2208 374 146 1280
Fragments arranged by size:
2208 1280 1064 839 374 146 138
EcoICRI GAG'CTC
Cuts at: 2716 2716
Size: 6049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2743 2930 4112 4709 5496 5764
Size: 393 580 531 187 1182 597 787 268
Cuts at: 5764 1239
Size: 1524
Fragments arranged by size:
1524 1182 787 597 580 531 393 268
187
EcoNI CCTnn'n_nnAGG
Cuts at: 2061 2061
Size: 6049
EcoO109I rG'GnC_Cy
Cuts at: 2078 2078
Size: 6049
EcoRI G'AATT_C
Cuts at: 2899 3358 3736 2899
Size: 459 378 5212
Fragments arranged by size:
5212 459 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2521 2066
Size: 455 5594
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2796 5196 1678
Size: 1118 2400 2531
Fragments arranged by size:
2531 2400 1118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4690 4722 4764 4796 1373
Size: 32 135 32 3118 32 42 32 2626
Fragments arranged by size:
3118 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 5163 5347 1305
Size: 1896 1962 184 2007
Fragments arranged by size:
2007 1962 1896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2538 10
Size: 54 1425 343 706 3521
Fragments arranged by size:
3521 1425 706 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2570 2601 3112 3215 4007 4366
Size: 648 949 455 31 511 103 792 359
Cuts at: 4366 4525 518
Size: 159 2042
Fragments arranged by size:
2042 949 792 648 511 455 359 159
103 31
MspA1I CmG'CkG
Cuts at: 1606 3289 3465 4142 5290 5535 1606
Size: 1683 176 677 1148 245 2120
Fragments arranged by size:
2120 1683 1148 677 245 176
NaeI GCC'GGC
Cuts at: 2670 2670
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2565 513
Size: 552 1500 3997
Fragments arranged by size:
3997 1500 552
NdeI CA'TA_TG
Cuts at: 387 2989 3256 387
Size: 2602 267 3180
Fragments arranged by size:
3180 2602 267
NgoMIV G'CCGG_C
Cuts at: 2668 2668
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2127 2141 2422 3407 4952 1132
Size: 97 898 14 281 985 1545 2229
Fragments arranged by size:
2229 1545 985 898 281 97 14
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 2137 4948 2137
Size: 2811 3238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 1108
Size: 1082 4967
PfoI T'CCnGG_A
Cuts at: 2056 2056
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2078 2078
Size: 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PstI C_TGCA'G
Cuts at: 830 3174 3401 830
Size: 2344 227 3478
Fragments arranged by size:
3478 2344 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
SacI G_AGCT'C
Cuts at: 2718 2718
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SapI GCTCTTCn'nnn_
Cuts at: 2495 2495
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3174 3401 3174
Size: 227 5822
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 3170 3397 4532 5213 5404 826
Size: 219 2125 227 1135 681 191 1471
Fragments arranged by size:
2125 1471 1135 681 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2073 2381 2506 3322 4153 5054
Size: 197 926 130 308 125 816 831 901
Cuts at: 5054 5316 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 831 816 308 262 197
130 125
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 2342 3973 5
Size: 47 2290 1631 2081
Fragments arranged by size:
2290 2081 1631 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2034 2270 2565 3488 513
Size: 552 969 236 295 923 3074
Fragments arranged by size:
3074 969 923 552 295 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4076 4093 4246 4431 4876 325
Size: 1255 2496 17 153 185 445 1498
Fragments arranged by size:
2496 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3148 3273 4295
Size: 274 80 33 51 1443 1171 125 1022
Cuts at: 4295 6007 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1171 1022 274 138 125 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2824 2856 2950 2982 6009 6041 2824
Size: 32 94 32 3027 32 2832
Fragments arranged by size:
3027 2832 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 2948 4178 2948
Size: 1230 4819
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI