Restriction map for
pFN21AB5514
(Circular) (Six-base) MAPSORT of: pFN21AB5514.seq Check: 4731 from: 1 to: 6046
pFN21AB5514 6046 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2342 2733 3389 1937
Size: 405 391 656 4594
Fragments arranged by size:
4594 656 405 391
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2841 3085 3200 4109 5493 5761 1632
Size: 1209 244 115 909 1384 268 1917
Fragments arranged by size:
1917 1384 1209 909 268 244 115
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 2920 4775 2920
Size: 1855 4191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2015 3789 5361 2015
Size: 1774 1572 2700
Fragments arranged by size:
2700 1774 1572
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 2108 2702 2879 2931 3038 3355 3581 3733
Size: 615 594 177 52 107 317 226 152
Cuts at: 3733 5842 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 615 594 317 226 177 152
107 52
AseI AT'TA_AT
Cuts at: 160 1032 2559 2604 4600 160
Size: 872 1527 45 1996 1606
Fragments arranged by size:
1996 1606 1527 872 45
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 3371 1870
Size: 73 1428 4545
Fragments arranged by size:
4545 1428 73
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3276 3367 4822 618
Size: 325 217 627 1489 91 1455 1842
Fragments arranged by size:
1842 1489 1455 627 325 217 91
BanII G_rGCy'C
Cuts at: 1391 3365 1391
Size: 1974 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 4107 5263 1790
Size: 2317 1156 2573
Fragments arranged by size:
2573 2317 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2823 3777 4735
Size: 416 53 83 399 24 1406 954 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1406 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2142 4706 1239
Size: 798 105 2564 2579
Fragments arranged by size:
2579 2564 798 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4709 882
Size: 617 3210 2219
Fragments arranged by size:
3210 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3853 4235 4870 275
Size: 53 83 186 936 2320 382 635 1451
Fragments arranged by size:
2320 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2034 2223 2432 2851 1186
Size: 848 189 209 419 4381
Fragments arranged by size:
4381 848 419 209 189
BseYI C'CCAG_C
Cuts at: 5249 5745 5249
Size: 496 5550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4107 4192 5263 4107
Size: 85 1071 4890
Fragments arranged by size:
4890 1071 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3365 4107 4192 5263 1391
Size: 399 1575 742 85 1071 2174
Fragments arranged by size:
2174 1575 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3075 3935 5665 3075
Size: 860 1730 3456
Fragments arranged by size:
3456 1730 860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3401 844
Size: 2557 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 4541 4715 65
Size: 1183 2046 1247 174 1396
Fragments arranged by size:
2046 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 2307 1184
Size: 1123 4923
BstYI r'GATC_y
Cuts at: 1972 2437 2485 2824 3059 3083 3376 3743 4128
Size: 465 48 339 235 24 293 367 385
Cuts at: 4128 4145 5597 5683 5695 6041 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 339 293
235 86 48 24 17 12
BstZ17I GTA'TAC
Cuts at: 2734 2734
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3132 487
Size: 683 56 1906 3401
Fragments arranged by size:
3401 1906 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3071 5053 809
Size: 507 1755 1982 1802
Fragments arranged by size:
1982 1802 1755 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3493 4382 6011
Size: 54 1425 50 275 18 1663 889 1629
Cuts at: 6011 8
Size: 43
Fragments arranged by size:
1663 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 3976 1353
Size: 1314 1309 3423
Fragments arranged by size:
3423 1314 1309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2142 2841 3085 3200 4109 4706
Size: 393 405 105 699 244 115 909 597
Cuts at: 4706 5493 5761 1239
Size: 787 268 1524
Fragments arranged by size:
1524 909 787 699 597 405 393 268
244 115 105
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
EcoRV GAT'ATC
Cuts at: 2653 2653
Size: 6046
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2454 2486 2906
Size: 32 135 32 576 32 274 32 420
Cuts at: 2906 2938 4687 4719 4761 4793 1373
Size: 32 1749 32 42 32 2626
Fragments arranged by size:
2626 1749 576 420 274 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3235 3390 3636 669
Size: 988 281 1297 155 246 3079
Fragments arranged by size:
3079 1297 988 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3032 5160 5344 1305
Size: 1727 2128 184 2007
Fragments arranged by size:
2128 2007 1727 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4224
Fragments arranged by size:
4224 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4004 4363 4522 518
Size: 648 2838 359 159 2042
Fragments arranged by size:
2838 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2718 3462 4139 5287 5532 1606
Size: 1112 744 677 1148 245 2120
Fragments arranged by size:
2120 1148 1112 744 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6046
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2989 3021 2989
Size: 32 6014
PstI C_TGCA'G
Cuts at: 830 2590 3398 830
Size: 1760 808 3478
Fragments arranged by size:
3478 1760 808
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2718 2718
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SapI GCTCTTCn'nnn_
Cuts at: 2667 2667
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SexAI A'CCwGG_T
Cuts at: 2973 2973
Size: 6046
SfcI C'TryA_G
Cuts at: 826 1045 2100 2445 2586 3394 4529 5210 5401
Size: 219 1055 345 141 808 1135 681 191
Cuts at: 5401 826
Size: 1471
Fragments arranged by size:
1471 1135 1055 808 681 345 219 191
141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 2517 3970 5
Size: 47 2465 1453 2081
Fragments arranged by size:
2465 2081 1453 47
StuI AGG'CCT
Cuts at: 1517 2479 2959 1517
Size: 962 480 4604
Fragments arranged by size:
4604 962 480
StyI C'CwwG_G
Cuts at: 513 1065 2004 3282 3485 513
Size: 552 939 1278 203 3074
Fragments arranged by size:
3074 1278 939 552 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2362 2394 6006 6038 2362
Size: 32 3612 32 2370
Fragments arranged by size:
3612 2370 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI