Restriction map for
pFN21AB5513
(Circular) (Six-base) MAPSORT of: pFN21AB5513.seq Check: 9242 from: 1 to: 6805
pFN21AB5513 6805 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4615 278
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4148 1937
Size: 2211 4594
Acc65I G'GTAC_C
Cuts at: 3863 4126 3863
Size: 263 6542
AclI AA'CG_TT
Cuts at: 1563 4932 5305 1563
Size: 3369 373 3063
Fragments arranged by size:
3369 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3703 3806 4868 6252 6520 1632
Size: 2071 103 1062 1384 268 1917
Fragments arranged by size:
2071 1917 1384 1062 268 103
AfeI AGC'GCT
Cuts at: 3386 3386
Size: 6805
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6608
AflIII A'CryG_T
Cuts at: 1203 2790 5704 6681 1203
Size: 1587 2914 977 1327
Fragments arranged by size:
2914 1587 1327 977
AgeI A'CCGG_T
Cuts at: 5636 5636
Size: 6805
AhdI GACnn_n'nnGTC
Cuts at: 5534 5534
Size: 6805
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6805
AlwNI CAG_nnn'CTG
Cuts at: 2776 2845 3166 4548 6120 2776
Size: 69 321 1382 1572 3461
Fragments arranged by size:
3461 1572 1382 321 69
ApaI G_GGCC'C
Cuts at: 2199 2199
Size: 6805
ApaLI G'TGCA_C
Cuts at: 4862 6018 4862
Size: 1156 5649
ApoI r'AATT_y
Cuts at: 1493 2972 3831 3871 4114 4340 4492 6601 1493
Size: 1479 859 40 243 226 152 2109 1697
Fragments arranged by size:
2109 1697 1479 859 243 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 5359 160
Size: 872 4327 1606
Fragments arranged by size:
4327 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2370 4046 4130 1870
Size: 73 271 156 1676 84 4545
Fragments arranged by size:
4545 1676 271 156 84 73
AvrII C'CTAG_G
Cuts at: 2308 2308
Size: 6805
BamHI G'GATC_C
Cuts at: 4042 4135 4502 4042
Size: 93 367 6345
Fragments arranged by size:
6345 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 3863 4126 5581 618
Size: 325 217 627 810 1266 263 1455 1842
Fragments arranged by size:
1842 1455 1266 810 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2063 2199 2308 3110 3237 4071 4124 1391
Size: 672 136 109 802 127 834 53 4072
Fragments arranged by size:
4072 834 802 672 136 127 109 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2625 928
Size: 971 726 5108
Fragments arranged by size:
5108 971 726
BbvCI CC'TCA_GC
Cuts at: 3347 3347
Size: 6805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4996 5030 1903
Size: 34 3059 34 3678
Fragments arranged by size:
3678 3059 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4697 5907 4697
Size: 1210 5595
BclI T'GATC_A
Cuts at: 1548 3168 1548
Size: 1620 5185
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 3626 5416
Size: 107 122 71 631 605 355 1599 1790
Cuts at: 5416 136
Size: 1525
Fragments arranged by size:
1790 1599 1525 631 605 355 122 107
71
BglII A'GATC_T
Cuts at: 2244 3021 3645 6800 2244
Size: 777 624 3155 2249
Fragments arranged by size:
3155 2249 777 624
BlpI GC'TnA_GC
Cuts at: 4222 4222
Size: 6805
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2790 4866 6022 1790
Size: 409 591 2076 1156 2573
Fragments arranged by size:
2573 2076 1156 591 409
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3616 4536
Size: 416 53 83 399 24 837 1362 920
Cuts at: 4536 5494 442
Size: 958 1753
Fragments arranged by size:
1753 1362 958 920 837 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2188 2554 2567 3038 3118 3197 5465
Size: 905 44 366 13 471 80 79 2268
Cuts at: 5465 1239
Size: 2579
Fragments arranged by size:
2579 2268 905 471 366 80 79 44
13
Bpu10I CC'TnA_GC
Cuts at: 3347 3347
Size: 6805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4930 5795 6093 4930
Size: 865 298 5642
Fragments arranged by size:
5642 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5468 882
Size: 617 3969 2219
Fragments arranged by size:
3969 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4596 493
Size: 711 3392 2702
Fragments arranged by size:
3392 2702 711
BsaBI GATnn'nnATC
Cuts at: 2852 6799 2852
Size: 3947 2858
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4612 4994 5629 275
Size: 53 83 186 936 3079 382 635 1451
Fragments arranged by size:
3079 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2627 2804 5238 5636 5910 6057 6468 2627
Size: 177 2434 398 274 147 411 2964
Fragments arranged by size:
2964 2434 411 398 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6580
Fragments arranged by size:
6580 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2289 2828 2948 3194 3197 3212 3332 1186
Size: 1103 539 120 246 3 15 120 4659
Fragments arranged by size:
4659 1103 539 246 120 120 15 3
BseYI C'CCAG_C
Cuts at: 2056 3778 6008 6504 2056
Size: 1722 2230 496 2357
Fragments arranged by size:
2357 2230 1722 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3202 1163
Size: 573 1466 4766
Fragments arranged by size:
4766 1466 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 4255 5016 6044 1540
Size: 275 186 48 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 3420 4071 4866 4951 6022 3420
Size: 651 795 85 1071 4203
Fragments arranged by size:
4203 1071 795 651 85
BsmI GAATG_Cn'
Cuts at: 2509 4316 4409 2509
Size: 1807 93 4905
Fragments arranged by size:
4905 1807 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2199 2308 2790 3110 3237 3420
Size: 399 273 136 109 482 320 127 183
Cuts at: 3420 4071 4124 4866 4951 6022 1391
Size: 651 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 482 399 320 273
183 136 127 109 85 53
BspEI T'CCGG_A
Cuts at: 6468 6468
Size: 6805
BspHI T'CATG_A
Cuts at: 4694 6424 4694
Size: 1730 5075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3896 4160 844
Size: 3052 264 3489
Fragments arranged by size:
3489 3052 264
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 4692 1394
Size: 429 1390 1479 3507
Fragments arranged by size:
3507 1479 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 2634 5300 5474 65
Size: 1183 1086 300 2666 174 1396
Fragments arranged by size:
2666 1396 1183 1086 300 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5449 5636 1700
Size: 110 3639 187 2869
Fragments arranged by size:
3639 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6805
BssSI C'ACGA_G
Cuts at: 2066 3455 4865 5877 2066
Size: 1389 1410 1012 2994
Fragments arranged by size:
2994 1410 1389 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2336 794
Size: 1542 5263
BstBI TT'CG_AA
Cuts at: 2616 3471 5609 2616
Size: 855 2138 3812
Fragments arranged by size:
3812 2138 855
BstEII G'GTnAC_C
Cuts at: 1184 2800 1184
Size: 1616 5189
BstYI r'GATC_y
Cuts at: 1972 2244 2415 3021 3645 4042 4135 4502 4887
Size: 272 171 606 624 397 93 367 385
Cuts at: 4887 4904 6356 6442 6454 6800 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 624 606 397 385 367 346
272 171 93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2999 2999
Size: 6805
BtgI C'CryG_G
Cuts at: 513 1065 2004 2238 2745 3114 513
Size: 552 939 234 507 369 4204
Fragments arranged by size:
4204 939 552 507 369 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1999 487
Size: 683 56 773 5293
Fragments arranged by size:
5293 773 683 56
BtsI GCAGTG_nn'
Cuts at: 4321 5115 5135 4321
Size: 794 20 5991
Fragments arranged by size:
5991 794 20
ClaI AT'CG_AT
Cuts at: 1554 3975 1554
Size: 2421 4384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6770
DraI TTT'AAA
Cuts at: 4109 4109
Size: 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5812 809
Size: 507 4496 1802
Fragments arranged by size:
4496 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2046 2160
Size: 54 1425 50 275 18 189 27 114
Cuts at: 2160 4252 5141 6770 8
Size: 2092 889 1629 43
Fragments arranged by size:
2092 1629 1425 889 275 189 114 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 4252 1537
Size: 275 234 2206 4090
Fragments arranged by size:
4090 2206 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3561 4735 1353
Size: 791 1417 1174 3423
Fragments arranged by size:
3423 1417 1174 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5392 5766 5912 387
Size: 1064 138 3803 374 146 1280
Fragments arranged by size:
3803 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4069 4069
Size: 6805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2188 2554 2567 3038 3118 3197
Size: 393 512 44 366 13 471 80 79
Cuts at: 3197 3703 3806 4868 5465 6252 6520 1239
Size: 506 103 1062 597 787 268 1524
Fragments arranged by size:
1524 1062 787 597 512 506 471 393
366 268 103 80 79 44 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 3410 2090
Size: 1320 5485
EcoO109I rG'GnC_Cy
Cuts at: 2146 2195 2196 2146
Size: 49 1 6755
Fragments arranged by size:
6755 49 1
EcoRI G'AATT_C
Cuts at: 2972 3871 4114 4492 2972
Size: 899 243 378 5285
Fragments arranged by size:
5285 899 378 243
EcoRV GAT'ATC
Cuts at: 3703 3703
Size: 6805
FspI TGC'GCA
Cuts at: 5311 5311
Size: 6805
HaeII r_GCGC'y
Cuts at: 1678 2108 2236 2541 2745 3388 3697 5952 1678
Size: 430 128 305 204 643 309 2255 2531
Fragments arranged by size:
2531 2255 643 430 309 305 204 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3142 3174 3692 3724 3907
Size: 32 135 32 1570 32 518 32 183
Cuts at: 3907 3939 4064 4096 5446 5478 5520 5552 1373
Size: 32 125 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1570 1350 518 183 135 125 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3748 3853 3926 4149 4395 669
Size: 988 281 1810 105 73 223 246 3079
Fragments arranged by size:
3079 1810 988 281 246 223 105 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6805
HpaI GTT'AAC
Cuts at: 4395 4395
Size: 6805
KpnI G_GTAC'C
Cuts at: 3867 4130 3867
Size: 263 6542
MfeI C'AATT_G
Cuts at: 4404 4404
Size: 6805
MluI A'CGCG_T
Cuts at: 2790 6681 2790
Size: 3891 2914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5919 6103 1305
Size: 4614 184 2007
Fragments arranged by size:
4614 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4983
Fragments arranged by size:
4983 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 3083 3455 4763 5122 5281 518
Size: 648 1798 119 372 1308 359 159 2042
Fragments arranged by size:
2042 1798 1308 648 372 359 159 119
MspA1I CmG'CkG
Cuts at: 1606 2747 3116 4039 4221 4898 6046 6291 1606
Size: 1141 369 923 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 1141 923 677 369 245 182
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5314
Fragments arranged by size:
5314 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6805
NotI GC'GGCC_GC
Cuts at: 4252 4252
Size: 6805
NspI r_CATG'y
Cuts at: 1132 1229 3283 3735 3754 4163 5708 1132
Size: 97 2054 452 19 409 1545 2229
Fragments arranged by size:
2229 2054 1545 452 409 97 19
PasI CC'CwG_GG
Cuts at: 1348 2176 2865 1348
Size: 828 689 5288
Fragments arranged by size:
5288 828 689
PciI A'CATG_T
Cuts at: 5704 5704
Size: 6805
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6805
PfoI T'CCnGG_A
Cuts at: 2142 2397 3408 2142
Size: 255 1011 5539
Fragments arranged by size:
5539 1011 255
PmeI GTTT'AAAC
Cuts at: 4109 4109
Size: 6805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4874 4906 6387 6419 4874
Size: 32 1481 32 5260
Fragments arranged by size:
5260 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2146
Size: 6805
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6805
PsiI TTA'TAA
Cuts at: 4375 4375
Size: 6805
PspOMI G'GGCC_C
Cuts at: 2195 2195
Size: 6805
PspXI vC'TCGA_Gb
Cuts at: 2370 2370
Size: 6805
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 6773
PstI C_TGCA'G
Cuts at: 830 3666 4157 830
Size: 2836 491 3478
Fragments arranged by size:
3478 2836 491
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6805
PvuII CAG'CTG
Cuts at: 4039 4039
Size: 6805
SacI G_AGCT'C
Cuts at: 4071 4071
Size: 6805
SacII CC_GC'GG
Cuts at: 2748 3117 2748
Size: 369 6436
SalI G'TCGA_C
Cuts at: 1936 4147 1936
Size: 2211 4594
SapI GCTCTTCn'nnn_
Cuts at: 2144 2144
Size: 6805
SbfI CC_TGCA'GG
Cuts at: 4157 4157
Size: 6805
ScaI AGT'ACT
Cuts at: 4101 5053 6765 4101
Size: 952 1712 4141
Fragments arranged by size:
4141 1712 952
SfcI C'TryA_G
Cuts at: 826 1045 2837 2922 3662 4153 5288 5969 6160
Size: 219 1792 85 740 491 1135 681 191
Cuts at: 6160 826
Size: 1471
Fragments arranged by size:
1792 1471 1135 740 681 491 219 191
85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6805
SmaI CCC'GGG
Cuts at: 1872 2216 4132 1872
Size: 344 1916 4545
Fragments arranged by size:
4545 1916 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2370 4909 5810 6072 820
Size: 197 926 427 2539 901 262 1553
Fragments arranged by size:
2539 1553 926 901 427 262 197
SnaBI TAC'GTA
Cuts at: 493 4596 493
Size: 4103 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6805
SphI G_CATG'C
Cuts at: 1132 4163 1132
Size: 3031 3774
SspI AAT'ATT
Cuts at: 5 52 4729 5
Size: 47 4677 2081
Fragments arranged by size:
4677 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6805
StyI C'CwwG_G
Cuts at: 513 1065 2004 2299 2308 2484 2716 4244 513
Size: 552 939 295 9 176 232 1528 3074
Fragments arranged by size:
3074 1528 939 552 295 232 176 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 2788 4832 4849 5002 5187 5632
Size: 1255 860 348 2044 17 153 185 445
Cuts at: 5632 325
Size: 1498
Fragments arranged by size:
2044 1498 1255 860 445 348 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3447 4099 5051
Size: 274 80 33 51 1443 1470 652 952
Cuts at: 5051 6763 96
Size: 1712 138
Fragments arranged by size:
1712 1470 1443 952 652 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6765 6797 6765
Size: 32 6773
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6805
XbaI T'CTAG_A
Cuts at: 4141 4141
Size: 6805
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2267 2267
Size: 6805
XhoI C'TCGA_G
Cuts at: 1943 2370 1943
Size: 427 6378
XmaI C'CCGG_G
Cuts at: 1870 2214 4130 1870
Size: 344 1916 4545
Fragments arranged by size:
4545 1916 344
XmnI GAAnn'nnTTC
Cuts at: 3143 3791 4934 3143
Size: 648 1143 5014
Fragments arranged by size:
5014 1143 648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4613 276
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII
SanDI SexAI SfiI SfoI SrfI SwaI