Restriction map for
pFN21AB5499
(Circular) (Six-base) MAPSORT of: pFN21AB5499.seq Check: 7503 from: 1 to: 9532
pFN21AB5499 9532 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2307 3700 2307
Size: 1393 8139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7342 278
Size: 53 83 186 936 5806 2468
Fragments arranged by size:
5806 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2946 6875 1937
Size: 1009 3929 4594
Fragments arranged by size:
4594 3929 1009
Acc65I G'GTAC_C
Cuts at: 6853 6853
Size: 9532
AclI AA'CG_TT
Cuts at: 1563 7659 8032 1563
Size: 6096 373 3063
Fragments arranged by size:
6096 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3339 3627 3862 4574 4956 4967 6072 6222
Size: 1707 288 235 712 382 11 1105 150
Cuts at: 6222 7595 8979 9247 1632
Size: 1373 1384 268 1917
Fragments arranged by size:
1917 1707 1384 1373 1105 712 382 288
268 235 150 11
AfeI AGC'GCT
Cuts at: 2150 4904 6307 2150
Size: 2754 1403 5375
Fragments arranged by size:
5375 2754 1403
AflII C'TTAA_G
Cuts at: 820 1017 3808 820
Size: 197 2791 6544
Fragments arranged by size:
6544 2791 197
AflIII A'CryG_T
Cuts at: 1203 2205 2207 8431 9408 1203
Size: 1002 2 6224 977 1327
Fragments arranged by size:
6224 1327 1002 977 2
AgeI A'CCGG_T
Cuts at: 4590 8363 4590
Size: 3773 5759
AhdI GACnn_n'nnGTC
Cuts at: 8261 8261
Size: 9532
AleI CACnn'nnGTG
Cuts at: 1166 2444 4984 1166
Size: 1278 2540 5714
Fragments arranged by size:
5714 2540 1278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4660 6729 1184
Size: 3476 2069 3987
Fragments arranged by size:
3987 3476 2069
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2546 2578 2546
Size: 32 9500
AlwNI CAG_nnn'CTG
Cuts at: 5740 6238 6344 7275 8847 5740
Size: 498 106 931 1572 6425
Fragments arranged by size:
6425 1572 931 498 106
ApaI G_GGCC'C
Cuts at: 2517 2517
Size: 9532
ApaLI G'TGCA_C
Cuts at: 2122 4378 7589 8745 2122
Size: 2256 3211 1156 2909
Fragments arranged by size:
3211 2909 2256 1156
ApoI r'AATT_y
Cuts at: 1493 2627 2900 3932 5296 6841 7067 7219 9328
Size: 1134 273 1032 1364 1545 226 152 2109
Cuts at: 9328 1493
Size: 1697
Fragments arranged by size:
2109 1697 1545 1364 1134 1032 273 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6555 8086 160
Size: 872 5523 1531 1606
Fragments arranged by size:
5523 1606 1531 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9532
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2159 2222 3471 3617 5757 6024
Size: 73 100 116 63 1249 146 2140 267
Cuts at: 6024 6857 1870
Size: 833 4545
Fragments arranged by size:
4545 2140 1249 833 267 146 116 100
73 63
BamHI G'GATC_C
Cuts at: 6862 7229 6862
Size: 367 9165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3131 3147 4298 4397 6623
Size: 325 217 627 1344 16 1151 99 2226
Cuts at: 6623 6853 8308 618
Size: 230 1455 1842
Fragments arranged by size:
2226 1842 1455 1344 1151 627 325 230
217 99 16
BanII G_rGCy'C
Cuts at: 1391 2517 3218 4422 4829 5831 6851 1391
Size: 1126 701 1204 407 1002 1020 4072
Fragments arranged by size:
4072 1204 1126 1020 1002 701 407
BbeI G_GCGC'C
Cuts at: 3135 3135
Size: 9532
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 2954 3789 3868 6791 928
Size: 971 615 440 835 79 2923 3669
Fragments arranged by size:
3669 2923 971 835 615 440 79
BbvCI CC'TCA_GC
Cuts at: 6545 6545
Size: 9532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6491 6525 7723 7757 1903
Size: 34 4554 34 1198 34 3678
Fragments arranged by size:
4554 3678 1198 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6070 6580 7424 8634 6070
Size: 510 844 1210 6968
Fragments arranged by size:
6968 1210 844 510
BclI T'GATC_A
Cuts at: 1548 5134 1548
Size: 3586 5946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 2047 2075
Size: 107 122 71 631 605 358 17 28
Cuts at: 2075 2090 3096 4878 5780 5816 6233 6266 6287
Size: 15 1006 1782 902 36 417 33 21
Cuts at: 6287 8143 136
Size: 1856 1525
Fragments arranged by size:
1856 1782 1525 1006 902 631 605 417
358 122 107 71 36 33 28 21
17 15
BglII A'GATC_T
Cuts at: 5802 6370 9527 5802
Size: 568 3157 5807
Fragments arranged by size:
5807 3157 568
BlpI GC'TnA_GC
Cuts at: 5820 5825 6949 5820
Size: 5 1124 8403
Fragments arranged by size:
8403 1124 5
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2126 2517 4382 5413 7593 8749 1790
Size: 314 22 391 1865 1031 2180 1156 2573
Fragments arranged by size:
2573 2180 1865 1156 1031 391 314 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2661 5196 7263
Size: 416 53 83 399 24 1244 2535 2067
Cuts at: 7263 8221 442
Size: 958 1753
Fragments arranged by size:
2535 2067 1753 1244 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9532
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3643 3933 5165 3643
Size: 290 1232 8010
Fragments arranged by size:
8010 1232 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4065 4353 5135 5870 5999 6707 6791 8192
Size: 2826 288 782 735 129 708 84 1401
Cuts at: 8192 1239
Size: 2579
Fragments arranged by size:
2826 2579 1401 782 735 708 288 129
84
Bpu10I CC'TnA_GC
Cuts at: 3834 6545 3834
Size: 2711 6821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2798 3815 5480 6477 7657 8522 8820 2438
Size: 360 1017 1665 997 1180 865 298 3150
Fragments arranged by size:
3150 1665 1180 1017 997 865 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3851 4190 4437 8195 882
Size: 617 2352 339 247 3758 2219
Fragments arranged by size:
3758 2352 2219 617 339 247
BsaAI yAC'GTr
Cuts at: 493 1204 2208 3974 4735 7323 493
Size: 711 1004 1766 761 2588 2702
Fragments arranged by size:
2702 2588 1766 1004 761 711
BsaBI GATnn'nnATC
Cuts at: 5441 9526 5441
Size: 4085 5447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 3132 7339 7721
Size: 53 83 186 936 750 849 4207 382
Cuts at: 7721 8356 275
Size: 635 1451
Fragments arranged by size:
4207 1451 936 849 750 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4590 5364 7965 8363 8637 8784 9195 4590
Size: 774 2601 398 274 147 411 4927
Fragments arranged by size:
4927 2601 774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 2546 2576 2775
Size: 30 165 30 978 30 135 30 199
Cuts at: 2775 2805 4361 4391 1178
Size: 30 1556 30 6319
Fragments arranged by size:
6319 1556 978 199 165 135 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3004 3541 4294 5783 6173 6533 6758 1186
Size: 1818 537 753 1489 390 360 225 3960
Fragments arranged by size:
3960 1818 1489 753 537 390 360 225
BseYI C'CCAG_C
Cuts at: 2393 2690 6228 6574 8735 9231 2393
Size: 297 3538 346 2161 496 2694
Fragments arranged by size:
3538 2694 2161 496 346 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2399 2405 4057 4285 4513 1163
Size: 573 522 141 6 1652 228 228 6182
Fragments arranged by size:
6182 1652 573 522 228 228 141 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 2144 2984 6153 6982 7743
Size: 275 186 84 59 840 3169 829 761
Cuts at: 7743 8771 1540
Size: 1028 2301
Fragments arranged by size:
3169 2301 1028 840 829 761 275 186
84 59
BsiHKAI G_wGCw'C
Cuts at: 2126 4033 4382 4422 7593 7678 8749 2126
Size: 1907 349 40 3171 85 1071 2909
Fragments arranged by size:
3171 2909 1907 1071 349 85 40
BsmI GAATG_Cn'
Cuts at: 3023 3059 3502 4553 5015 6325 7043 7136 3023
Size: 36 443 1051 462 1310 718 93 5419
Fragments arranged by size:
5419 1310 1051 718 462 443 93 36
BsmBI CGTCTCn'nnnn_
Cuts at: 2456 2456
Size: 9532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2126 2517 3218 4033 4382 4422
Size: 399 314 22 391 701 815 349 40
Cuts at: 4422 4829 5413 5831 6851 7593 7678 8749 1391
Size: 407 584 418 1020 742 85 1071 2174
Fragments arranged by size:
2174 1071 1020 815 742 701 584 418
407 399 391 349 314 85 40 22
BspEI T'CCGG_A
Cuts at: 5364 9195 5364
Size: 3831 5701
BspHI T'CATG_A
Cuts at: 6750 7421 9151 6750
Size: 671 1730 7131
Fragments arranged by size:
7131 1730 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 3700 5652 6887 844
Size: 1463 1393 1952 1235 3489
Fragments arranged by size:
3489 1952 1463 1393 1235
BsrBI CCG'CTC
Cuts at: 1394 1823 5431 7419 1394
Size: 429 3608 1988 3507
Fragments arranged by size:
3608 3507 1988 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3043 3681 4189 8027 8201 65
Size: 1183 1795 638 508 3838 174 1396
Fragments arranged by size:
3838 1795 1396 1183 638 508 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2979 4590 4856 5109 5783 8176
Size: 110 219 950 1611 266 253 674 2393
Cuts at: 8176 8363 1700
Size: 187 2869
Fragments arranged by size:
2869 2393 1611 950 674 266 253 219
187 110
BsrGI T'GTAC_A
Cuts at: 96 3515 96
Size: 3419 6113
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9532
BssSI C'ACGA_G
Cuts at: 7592 8604 7592
Size: 1012 8520
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3336 3486 4653 794
Size: 2542 150 1167 5673
Fragments arranged by size:
5673 2542 1167 150
BstBI TT'CG_AA
Cuts at: 8336 8336
Size: 9532
BstEII G'GTnAC_C
Cuts at: 1184 2248 2257 1184
Size: 1064 9 8459
Fragments arranged by size:
8459 1064 9
BstXI CCAn_nnnn'nTGG
Cuts at: 4751 4751
Size: 9532
BstYI r'GATC_y
Cuts at: 1972 4970 5737 5802 6370 6862 7229 7614 7631
Size: 2998 767 65 568 492 367 385 17
Cuts at: 7631 9083 9169 9181 9527 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2998 1977 1452 767 568 492 385 367
346 86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 4965 4965
Size: 9532
BtgI C'CryG_G
Cuts at: 513 1065 2088 2112 2575 2984 3091 513
Size: 552 1023 24 463 409 107 6954
Fragments arranged by size:
6954 1023 552 463 409 107 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8793
Fragments arranged by size:
8793 683 56
BtsI GCAGTG_nn'
Cuts at: 4273 4501 4569 4721 4847 6250 7048 7842 7862
Size: 228 68 152 126 1403 798 794 20
Cuts at: 7862 4273
Size: 5943
Fragments arranged by size:
5943 1403 798 794 228 152 126 68
20
ClaI AT'CG_AT
Cuts at: 1554 4506 1554
Size: 2952 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2191 2226 3955 3990 4044 4079 4353
Size: 35 1627 35 1729 35 54 35 274
Cuts at: 4353 4388 529
Size: 35 5673
Fragments arranged by size:
5673 1729 1627 274 54 35 35 35
35 35
DraI TTT'AAA
Cuts at: 6836 6836
Size: 9532
DraIII CAC_nnn'GTG
Cuts at: 2355 3231 3233 2355
Size: 876 2 8654
Fragments arranged by size:
8654 876 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4158 8539 809
Size: 507 2842 4381 1802
Fragments arranged by size:
4381 2842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2082 2141
Size: 54 1425 50 275 18 237 15 59
Cuts at: 2141 2196 2376 2981 3088 4741 4858 6150 6979
Size: 55 180 605 107 1653 117 1292 829
Cuts at: 6979 7868 9497 8
Size: 889 1629 43
Fragments arranged by size:
1653 1629 1425 1292 889 829 605 275
237 180 117 107 59 55 54 50
43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2082 2141 2981 6150 6979 1537
Size: 275 270 59 840 3169 829 4090
Fragments arranged by size:
4090 3169 840 829 275 270 59
EarI CTCTTCn'nnn_
Cuts at: 1353 3196 4126 5373 5541 5688 7462 1353
Size: 1843 930 1247 168 147 1774 3423
Fragments arranged by size:
3423 1843 1774 1247 930 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2208 3155 6269 6305 8119 8493
Size: 1064 138 619 947 3114 36 1814 374
Cuts at: 8493 8639 387
Size: 146 1280
Fragments arranged by size:
3114 1814 1280 1064 947 619 374 146
138 36
EcoICRI GAG'CTC
Cuts at: 4420 4420
Size: 9532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3339 3627 3862 4065 4353 4574 4956
Size: 393 1707 288 235 203 288 221 382
Cuts at: 4956 4967 5135 5870 5999 6072 6222 6707 6791
Size: 11 168 735 129 73 150 485 84
Cuts at: 6791 7595 8192 8979 9247 1239
Size: 804 597 787 268 1524
Fragments arranged by size:
1707 1524 804 787 735 597 485 393
382 288 288 268 235 221 203 168
150 129 84 73 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3631 4212 5264 3631
Size: 581 1052 7899
Fragments arranged by size:
7899 1052 581
EcoO109I rG'GnC_Cy
Cuts at: 2514 5518 5929 6043 6235 2514
Size: 3004 411 114 192 5811
Fragments arranged by size:
5811 3004 411 192 114
EcoRI G'AATT_C
Cuts at: 2627 6841 7219 2627
Size: 4214 378 4940
Fragments arranged by size:
4940 4214 378
EcoRV GAT'ATC
Cuts at: 3643 6739 3643
Size: 3096 6436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3892 3892
Size: 9532
FspI TGC'GCA
Cuts at: 8038 8038
Size: 9532
HaeII r_GCGC'y
Cuts at: 1678 2152 2697 3135 4906 6309 8679 1678
Size: 474 545 438 1771 1403 2370 2531
Fragments arranged by size:
2531 2370 1771 1403 545 474 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3709 3741 4189 4221 4404
Size: 32 135 32 2137 32 448 32 183
Cuts at: 4404 4436 8173 8205 8247 8279 1373
Size: 32 3737 32 42 32 2626
Fragments arranged by size:
3737 2626 2137 448 183 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 4915 5290 5628 6876 7122
Size: 988 281 232 2745 375 338 1248 246
Cuts at: 7122 669
Size: 3079
Fragments arranged by size:
3079 2745 1248 988 375 338 281 246
232
HindIII A'AGCT_T
Cuts at: 748 2427 5558 748
Size: 1679 3131 4722
Fragments arranged by size:
4722 3131 1679
HpaI GTT'AAC
Cuts at: 7122 7122
Size: 9532
KasI G'GCGC_C
Cuts at: 3131 3131
Size: 9532
KpnI G_GTAC'C
Cuts at: 6857 6857
Size: 9532
MfeI C'AATT_G
Cuts at: 3421 4657 7131 3421
Size: 1236 2474 5822
Fragments arranged by size:
5822 2474 1236
MluI A'CGCG_T
Cuts at: 9408 9408
Size: 9532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4501 8646 8830 1305
Size: 3196 4145 184 2007
Fragments arranged by size:
4145 3196 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 3090 4743 10
Size: 54 1425 343 366 892 1653 4799
Fragments arranged by size:
4799 1653 1425 892 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 4984 5873 6464 7490 7849 8008
Size: 648 1278 2540 889 591 1026 359 159
Cuts at: 8008 518
Size: 2042
Fragments arranged by size:
2540 2042 1278 1026 889 648 591 359
159
MspA1I CmG'CkG
Cuts at: 1606 2090 2577 3255 4695 5824 6574 6583 6948
Size: 484 487 678 1440 1129 750 9 365
Cuts at: 6948 7625 8773 9018 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1440 1148 1129 750 678 677 487
484 365 245 9
NaeI GCC'GGC
Cuts at: 2031 2031
Size: 9532
NarI GG'CG_CC
Cuts at: 3132 3132
Size: 9532
NcoI C'CATG_G
Cuts at: 513 1065 2112 3091 513
Size: 552 1047 979 6954
Fragments arranged by size:
6954 1047 979 552
NdeI CA'TA_TG
Cuts at: 387 2938 387
Size: 2551 6981
NgoMIV G'CCGG_C
Cuts at: 2029 2029
Size: 9532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9532
NotI GC'GGCC_GC
Cuts at: 2082 2141 6979 2082
Size: 59 4838 4635
Fragments arranged by size:
4838 4635 59
NspI r_CATG'y
Cuts at: 1132 1229 2158 6890 8435 1132
Size: 97 929 4732 1545 2229
Fragments arranged by size:
4732 2229 1545 929 97
PasI CC'CwG_GG
Cuts at: 1348 4450 6238 1348
Size: 3102 1788 4642
Fragments arranged by size:
4642 3102 1788
PciI A'CATG_T
Cuts at: 8431 8431
Size: 9532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2355 4822 1108
Size: 1247 2467 5818
Fragments arranged by size:
5818 2467 1247
PfoI T'CCnGG_A
Cuts at: 4330 5151 4330
Size: 821 8711
PmeI GTTT'AAAC
Cuts at: 6836 6836
Size: 9532
PmlI CAC'GTG
Cuts at: 2208 4735 2208
Size: 2527 7005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2546 2578 7601 7633 9114 9146 2546
Size: 32 5023 32 1481 32 2932
Fragments arranged by size:
5023 2932 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5518 5929 5518
Size: 411 9121
PshAI GACnn'nnGTC
Cuts at: 1613 2255 1613
Size: 642 8890
PsiI TTA'TAA
Cuts at: 7102 7102
Size: 9532
PspOMI G'GGCC_C
Cuts at: 2513 2513
Size: 9532
PstI C_TGCA'G
Cuts at: 830 2304 2727 4232 4297 4785 6411 6884 830
Size: 1474 423 1505 65 488 1626 473 3478
Fragments arranged by size:
3478 1626 1505 1474 488 473 423 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9532
PvuII CAG'CTG
Cuts at: 3255 4695 5824 6574 6583 3255
Size: 1440 1129 750 9 6204
Fragments arranged by size:
6204 1440 1129 750 9
SacI G_AGCT'C
Cuts at: 4422 4422
Size: 9532
SacII CC_GC'GG
Cuts at: 2091 2578 2091
Size: 487 9045
SalI G'TCGA_C
Cuts at: 1936 6874 1936
Size: 4938 4594
SanDI GG'GwC_CC
Cuts at: 5518 5518
Size: 9532
SapI GCTCTTCn'nnn_
Cuts at: 3196 5688 3196
Size: 2492 7040
SbfI CC_TGCA'GG
Cuts at: 6884 6884
Size: 9532
ScaI AGT'ACT
Cuts at: 2534 7780 9492 2534
Size: 5246 1712 2574
Fragments arranged by size:
5246 2574 1712
SexAI A'CCwGG_T
Cuts at: 2351 2351
Size: 9532
SfcI C'TryA_G
Cuts at: 826 1045 2300 2536 2723 2847 3202 3532 4228
Size: 219 1255 236 187 124 355 330 696
Cuts at: 4228 4293 4781 5312 5511 6407 6880 8015 8696
Size: 65 488 531 199 896 473 1135 681
Cuts at: 8696 8887 826
Size: 191 1471
Fragments arranged by size:
1471 1255 1135 896 696 681 531 488
473 355 330 236 219 199 191 187
124 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 2030 3096 5780 2030
Size: 1066 2684 5782
Fragments arranged by size:
5782 2684 1066
SfoI GGC'GCC
Cuts at: 3133 3133
Size: 9532
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9532
SmaI CCC'GGG
Cuts at: 1872 2045 6859 1872
Size: 173 4814 4545
Fragments arranged by size:
4814 4545 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2453 2813 3617 3794 3808 5459
Size: 197 926 510 360 804 177 14 1651
Cuts at: 5459 6492 7636 8537 8799 820
Size: 1033 1144 901 262 1553
Fragments arranged by size:
1651 1553 1144 1033 926 901 804 510
360 262 197 177 14
SnaBI TAC'GTA
Cuts at: 493 7323 493
Size: 6830 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9532
SphI G_CATG'C
Cuts at: 1132 2158 6890 1132
Size: 1026 4732 3774
Fragments arranged by size:
4732 3774 1026
SspI AAT'ATT
Cuts at: 5 52 7456 5
Size: 47 7404 2081
Fragments arranged by size:
7404 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 3913 1517
Size: 1095 1301 7136
Fragments arranged by size:
7136 1301 1095
StyI C'CwwG_G
Cuts at: 513 1065 2112 2565 3091 3169 3905 4821 5179
Size: 552 1047 453 526 78 736 916 358
Cuts at: 5179 6039 6333 6971 513
Size: 860 294 638 3074
Fragments arranged by size:
3074 1047 916 860 736 638 552 526
453 358 294 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 3567 4051 5061 7559 7576 7729
Size: 1255 759 1228 484 1010 2498 17 153
Cuts at: 7729 7914 8359 325
Size: 185 445 1498
Fragments arranged by size:
2498 1498 1255 1228 1010 759 484 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 2618 3515
Size: 274 80 33 51 1443 555 86 897
Cuts at: 3515 5761 7778 9490 96
Size: 2246 2017 1712 138
Fragments arranged by size:
2246 2017 1712 1443 897 555 274 138
86 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 3970 4002 4361 4372 4393 4404 9492
Size: 32 1163 32 359 11 21 11 5088
Cuts at: 9492 9524 2775
Size: 32 2783
Fragments arranged by size:
5088 2783 1163 359 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 3407 1316
Size: 2091 7441
XbaI T'CTAG_A
Cuts at: 6868 6868
Size: 9532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2537 4925 2537
Size: 2388 7144
XhoI C'TCGA_G
Cuts at: 1943 3617 1943
Size: 1674 7858
XmaI C'CCGG_G
Cuts at: 1870 2043 6857 1870
Size: 173 4814 4545
Fragments arranged by size:
4814 4545 173
XmnI GAAnn'nnTTC
Cuts at: 3161 7661 3161
Size: 4500 5032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7340 276
Size: 53 83 186 936 5806 2468
Fragments arranged by size:
5806 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI