Restriction map for
pFN21AB5493
(Circular) (Six-base) MAPSORT of: pFN21AB5493.seq Check: 981 from: 1 to: 7429
pFN21AB5493 7429 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2632 3539 2632
Size: 907 6522
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5239 278
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4772 1937
Size: 2835 4594
Acc65I G'GTAC_C
Cuts at: 4208 4750 4208
Size: 542 6887
AclI AA'CG_TT
Cuts at: 1563 5556 5929 1563
Size: 3993 373 3063
Fragments arranged by size:
3993 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2259 2342 2814 3605 3845 5492 6876
Size: 531 96 83 472 791 240 1647 1384
Cuts at: 6876 7144 1632
Size: 268 1917
Fragments arranged by size:
1917 1647 1384 791 531 472 268 240
96 83
AfeI AGC'GCT
Cuts at: 2226 2226
Size: 7429
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7232
AflIII A'CryG_T
Cuts at: 1203 6328 7305 1203
Size: 5125 977 1327
Fragments arranged by size:
5125 1327 977
AgeI A'CCGG_T
Cuts at: 6260 6260
Size: 7429
AhdI GACnn_n'nnGTC
Cuts at: 6158 6158
Size: 7429
AleI CACnn'nnGTG
Cuts at: 1166 2564 3785 1166
Size: 1398 1221 4810
Fragments arranged by size:
4810 1398 1221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2707 1184
Size: 1523 5906
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3107 3139 3948 3980 3107
Size: 32 809 32 6556
Fragments arranged by size:
6556 809 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2137 2521 3647 3812 3838 4306 4532 5172 6744
Size: 384 1126 165 26 468 226 640 1572
Cuts at: 6744 2137
Size: 2822
Fragments arranged by size:
2822 1572 1126 640 468 384 226 165
26
ApaLI G'TGCA_C
Cuts at: 5486 6642 5486
Size: 1156 6273
ApoI r'AATT_y
Cuts at: 1493 2294 4139 4738 4964 5116 7225 1493
Size: 801 1845 599 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 801 599 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5983 160
Size: 872 4951 1606
Fragments arranged by size:
4951 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
AvaI C'yCGr_G
Cuts at: 1870 1943 3006 4754 1870
Size: 73 1063 1748 4545
Fragments arranged by size:
4545 1748 1063 73
BamHI G'GATC_C
Cuts at: 4759 5126 4759
Size: 367 7062
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3034 4208 4325 4750
Size: 325 217 627 242 1005 1174 117 425
Cuts at: 4750 6205 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1174 1005 627 425 325 242
217 117
BanII G_rGCy'C
Cuts at: 1391 2444 3030 3337 3654 4748 1391
Size: 1053 586 307 317 1094 4072
Fragments arranged by size:
4072 1094 1053 586 317 307
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 7429
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2847 3891 4589 928
Size: 971 948 1044 698 3768
Fragments arranged by size:
3768 1044 971 948 698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5620 5654 1903
Size: 34 3683 34 3678
Fragments arranged by size:
3683 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2197 3652 4120 5321 6531 2197
Size: 1455 468 1201 1210 3095
Fragments arranged by size:
3095 1455 1210 1201 468
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7429
BfrBI ATG'CAT
Cuts at: 2678 2678
Size: 7429
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3725 4226 4572
Size: 107 122 71 631 605 2053 501 346
Cuts at: 4572 6040 136
Size: 1468 1525
Fragments arranged by size:
2053 1525 1468 631 605 501 346 122
107 71
BglII A'GATC_T
Cuts at: 7424 7424
Size: 7429
BlpI GC'TnA_GC
Cuts at: 4846 4846
Size: 7429
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2761 4328 5490 6646 1790
Size: 493 478 1567 1162 1156 2573
Fragments arranged by size:
2573 1567 1162 1156 493 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3698 4610 5160
Size: 416 53 83 399 24 2281 912 550
Cuts at: 5160 6118 442
Size: 958 1753
Fragments arranged by size:
2281 1753 958 912 550 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2192 2249 2675 2939 3764 6089 1239
Size: 798 155 57 426 264 825 2325 2579
Fragments arranged by size:
2579 2325 825 798 426 264 155 57
Bpu10I CC'TnA_GC
Cuts at: 2505 2727 3001 4133 2505
Size: 222 274 1132 5801
Fragments arranged by size:
5801 1132 274 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3638 5554 6419 6717 2408
Size: 1230 1916 865 298 3120
Fragments arranged by size:
3120 1916 1230 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3698 6092 882
Size: 617 2199 2394 2219
Fragments arranged by size:
2394 2219 2199 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 5220 493
Size: 711 1518 2498 2702
Fragments arranged by size:
2702 2498 1518 711
BsaBI GATnn'nnATC
Cuts at: 4155 7423 4155
Size: 3268 4161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 5236 5618 6253
Size: 53 83 186 936 497 3206 382 635
Cuts at: 6253 275
Size: 1451
Fragments arranged by size:
3206 1451 936 635 497 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3369 4062 5862 6260 6534 6681 7092 3369
Size: 693 1800 398 274 147 411 3706
Fragments arranged by size:
3706 1800 693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 2402 2432 3107
Size: 30 165 30 963 30 6 30 675
Cuts at: 3107 3137 3290 3320 3737 3767 3851 3881 3948
Size: 30 153 30 417 30 84 30 67
Cuts at: 3948 3978 1178
Size: 30 4629
Fragments arranged by size:
4629 963 675 417 165 153 84 67
30 30 30 30 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2954 3115 3298 4010 4523 4673 1186
Size: 838 930 161 183 712 513 150 3942
Fragments arranged by size:
3942 930 838 712 513 183 161 150
BseYI C'CCAG_C
Cuts at: 2043 6632 7128 2043
Size: 4589 496 2344
Fragments arranged by size:
4589 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 3252 3723 3851 4026 1163
Size: 573 321 1195 471 128 175 4566
Fragments arranged by size:
4566 1195 573 471 321 175 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4357 4879 5640 6668 1540
Size: 275 186 2356 522 761 1028 2301
Fragments arranged by size:
2356 2301 1028 761 522 275 186
BsiHKAI G_wGCw'C
Cuts at: 3337 3654 3949 5490 5575 6646 3337
Size: 317 295 1541 85 1071 4120
Fragments arranged by size:
4120 1541 1071 317 295 85
BsmI GAATG_Cn'
Cuts at: 2888 3154 4494 4940 5033 2888
Size: 266 1340 446 93 5284
Fragments arranged by size:
5284 1340 446 266 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2444 2761 3030 3337 3654 3949
Size: 399 493 161 317 269 307 317 295
Cuts at: 3949 4328 4748 5490 5575 6646 1391
Size: 379 420 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 420 399 379 317
317 307 295 269 161 85
BspEI T'CCGG_A
Cuts at: 3369 4062 7092 3369
Size: 693 3030 3706
Fragments arranged by size:
3706 3030 693
BspHI T'CATG_A
Cuts at: 2470 4147 5318 7048 2470
Size: 1677 1171 1730 2851
Fragments arranged by size:
2851 1730 1677 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2631 2632 3539 4784 844
Size: 1787 1 907 1245 3489
Fragments arranged by size:
3489 1787 1245 907 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 2101 5316 1394
Size: 429 268 10 3215 3507
Fragments arranged by size:
3507 3215 429 268 10
BsrDI GCAATG_nn'
Cuts at: 65 1248 3601 4239 4520 5924 6098 65
Size: 1183 2353 638 281 1404 174 1396
Fragments arranged by size:
2353 1404 1396 1183 638 281 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 2901 4356 6073 6260 1700
Size: 110 222 869 1455 1717 187 2869
Fragments arranged by size:
2869 1717 1455 869 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 2974 96
Size: 2878 4551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7429
BssSI C'ACGA_G
Cuts at: 2180 5489 6501 2180
Size: 3309 1012 3108
Fragments arranged by size:
3309 3108 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3838 794
Size: 3044 4385
BstBI TT'CG_AA
Cuts at: 4423 6233 4423
Size: 1810 5619
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7429
BstXI CCAn_nnnn'nTGG
Cuts at: 3784 4195 3784
Size: 411 7018
BstYI r'GATC_y
Cuts at: 1972 4759 5126 5511 5528 6980 7066 7078 7424
Size: 2787 367 385 17 1452 86 12 346
Cuts at: 7424 1972
Size: 1977
Fragments arranged by size:
2787 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3217 4188 4380 4630 513
Size: 552 939 1213 971 192 250 3312
Fragments arranged by size:
3312 1213 971 939 552 250 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6690
Fragments arranged by size:
6690 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3395 3500 4210 4557 4945 5739 5759 3091
Size: 304 105 710 347 388 794 20 4761
Fragments arranged by size:
4761 794 710 388 347 304 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7429
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7394
DraI TTT'AAA
Cuts at: 4733 4733
Size: 7429
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6436 809
Size: 507 5120 1802
Fragments arranged by size:
5120 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 3160 4030
Size: 54 1425 50 275 18 561 769 870
Cuts at: 4030 4377 4517 4650 4876 5765 7394 8
Size: 347 140 133 226 889 1629 43
Fragments arranged by size:
1629 1425 889 870 769 561 347 275
226 140 133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4876 1537
Size: 275 3064 4090
Fragments arranged by size:
4090 3064 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 3342 4286 5359 1353
Size: 786 1203 944 1073 3423
Fragments arranged by size:
3423 1203 1073 944 786
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6016 6390 6536 387
Size: 1064 138 4427 374 146 1280
Fragments arranged by size:
4427 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3335 3652 3335
Size: 317 7112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2163 2192 2249 2259 2342 2675
Size: 393 405 126 29 57 10 83 333
Cuts at: 2675 2814 2939 3605 3764 3845 5492 6089 6876
Size: 139 125 666 159 81 1647 597 787
Cuts at: 6876 7144 1239
Size: 268 1524
Fragments arranged by size:
1647 1524 787 666 597 405 393 333
268 159 139 126 125 83 81 57
29 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3707 3707
Size: 7429
EcoO109I rG'GnC_Cy
Cuts at: 2950 3588 4267 4303 2950
Size: 638 679 36 6076
Fragments arranged by size:
6076 679 638 36
EcoRI G'AATT_C
Cuts at: 4738 5116 4738
Size: 378 7051
FspI TGC'GCA
Cuts at: 5935 5935
Size: 7429
HaeII r_GCGC'y
Cuts at: 1678 2033 2228 6576 1678
Size: 355 195 4348 2531
Fragments arranged by size:
4348 2531 355 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2951 2983 3515
Size: 32 135 32 794 32 553 32 532
Cuts at: 3515 3547 3678 3710 3737 3769 4104 4136 6070
Size: 32 131 32 27 32 335 32 1934
Cuts at: 6070 6102 6144 6176 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1934 794 553 532 335 135 131
42 32 32 32 32 32 32 32
32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 4773 5019 669
Size: 988 281 2835 246 3079
Fragments arranged by size:
3079 2835 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7429
HpaI GTT'AAC
Cuts at: 5019 5019
Size: 7429
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 7429
KpnI G_GTAC'C
Cuts at: 4212 4754 4212
Size: 542 6887
MfeI C'AATT_G
Cuts at: 5028 5028
Size: 7429
MluI A'CGCG_T
Cuts at: 7305 7305
Size: 7429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6543 6727 1305
Size: 5238 184 2007
Fragments arranged by size:
5238 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 3162 4032 4652 10
Size: 54 1425 343 561 769 870 620 2787
Fragments arranged by size:
2787 1425 870 769 620 561 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2475 2564 3542 3785 4193 5387
Size: 648 1233 76 89 978 243 408 1194
Cuts at: 5387 5746 5905 518
Size: 359 159 2042
Fragments arranged by size:
2042 1233 1194 978 648 408 359 243
159 89 76
MspA1I CmG'CkG
Cuts at: 1606 2057 2545 3236 3838 4845 5522 6670 6915
Size: 451 488 691 602 1007 677 1148 245
Cuts at: 6915 1606
Size: 2120
Fragments arranged by size:
2120 1148 1007 691 677 602 488 451
245
NaeI GCC'GGC
Cuts at: 2903 2903
Size: 7429
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 7429
NcoI C'CATG_G
Cuts at: 513 1065 2004 4188 513
Size: 552 939 2184 3754
Fragments arranged by size:
3754 2184 939 552
NdeI CA'TA_TG
Cuts at: 387 2680 3802 387
Size: 2293 1122 4014
Fragments arranged by size:
4014 2293 1122
NgoMIV G'CCGG_C
Cuts at: 2901 2901
Size: 7429
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7429
NotI GC'GGCC_GC
Cuts at: 4876 4876
Size: 7429
NsiI A_TGCA'T
Cuts at: 2680 2680
Size: 7429
NspI r_CATG'y
Cuts at: 1132 1229 2653 4787 6332 1132
Size: 97 1424 2134 1545 2229
Fragments arranged by size:
2229 2134 1545 1424 97
PasI CC'CwG_GG
Cuts at: 1348 2348 2761 1348
Size: 1000 413 6016
Fragments arranged by size:
6016 1000 413
PciI A'CATG_T
Cuts at: 6328 6328
Size: 7429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2312 4552 1108
Size: 1204 2240 3985
Fragments arranged by size:
3985 2240 1204
PfoI T'CCnGG_A
Cuts at: 2916 4122 4413 2916
Size: 1206 291 5932
Fragments arranged by size:
5932 1206 291
PmeI GTTT'AAAC
Cuts at: 4733 4733
Size: 7429
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3427 3459 3948 3980 5498 5530 7011
Size: 32 288 32 489 32 1518 32 1481
Cuts at: 7011 7043 3107
Size: 32 3493
Fragments arranged by size:
3493 1518 1481 489 288 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2950 3588 4267 2950
Size: 638 679 6112
Fragments arranged by size:
6112 679 638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7429
PsiI TTA'TAA
Cuts at: 4999 4999
Size: 7429
PstI C_TGCA'G
Cuts at: 830 4781 830
Size: 3951 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7429
PvuII CAG'CTG
Cuts at: 3236 3838 3236
Size: 602 6827
SacI G_AGCT'C
Cuts at: 3337 3654 3337
Size: 317 7112
SalI G'TCGA_C
Cuts at: 1936 4771 1936
Size: 2835 4594
SapI GCTCTTCn'nnn_
Cuts at: 3342 3342
Size: 7429
SbfI CC_TGCA'GG
Cuts at: 4781 4781
Size: 7429
ScaI AGT'ACT
Cuts at: 5677 7389 5677
Size: 1712 5717
SfcI C'TryA_G
Cuts at: 826 1045 4777 5912 6593 6784 826
Size: 219 3732 1135 681 191 1471
Fragments arranged by size:
3732 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 7429
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7429
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 7097
SmaI CCC'GGG
Cuts at: 1872 4756 1872
Size: 2884 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 3653 5533 6434 6696 820
Size: 197 926 480 1230 1880 901 262 1553
Fragments arranged by size:
1880 1553 1230 926 901 480 262 197
SnaBI TAC'GTA
Cuts at: 493 5220 493
Size: 4727 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7429
SphI G_CATG'C
Cuts at: 1132 4787 1132
Size: 3655 3774
SspI AAT'ATT
Cuts at: 5 52 5353 5
Size: 47 5301 2081
Fragments arranged by size:
5301 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7429
StyI C'CwwG_G
Cuts at: 513 1065 2004 3030 4188 4263 4868 513
Size: 552 939 1026 1158 75 605 3074
Fragments arranged by size:
3074 1158 1026 939 605 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2753 3299 5456 5473 5626 5811 6256
Size: 1255 1173 546 2157 17 153 185 445
Cuts at: 6256 325
Size: 1498
Fragments arranged by size:
2157 1498 1255 1173 546 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2872 2974 3303
Size: 274 80 33 51 1443 895 102 329
Cuts at: 3303 5675 7387 96
Size: 2372 1712 138
Fragments arranged by size:
2372 1712 1443 895 329 274 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2434 3364 3396 3851 3883 7389 7421 2402
Size: 32 930 32 455 32 3506 32 2410
Fragments arranged by size:
3506 2410 930 455 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3690 1316
Size: 2374 5055
XbaI T'CTAG_A
Cuts at: 4765 4765
Size: 7429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3541 3784 4186 4195 3541
Size: 243 402 9 6775
Fragments arranged by size:
6775 402 243 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7429
XmaI C'CCGG_G
Cuts at: 1870 4754 1870
Size: 2884 4545
XmnI GAAnn'nnTTC
Cuts at: 5558 5558
Size: 7429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5237 276
Size: 53 83 186 936 3703 2468
Fragments arranged by size:
3703 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI