Restriction map for
pFN21AB5490
(Circular) (Six-base) MAPSORT of: pFN21AB5490.seq Check: 7693 from: 1 to: 6187
pFN21AB5490 6187 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2634 4250 5634 5902 1632
Size: 710 292 1616 1384 268 1917
Fragments arranged by size:
1917 1616 1384 710 292 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 2060 3258 5086 6063 1203
Size: 857 1198 1828 977 1327
Fragments arranged by size:
1828 1327 1198 977 857
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 3175 3332 4916 3175
Size: 157 1584 4446
Fragments arranged by size:
4446 1584 157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 2473 2587 2741 2984 3930 5502 2473
Size: 114 154 243 946 1572 3158
Fragments arranged by size:
3158 1572 946 243 154 114
ApaLI G'TGCA_C
Cuts at: 3087 4244 5400 3087
Size: 1157 1156 3874
Fragments arranged by size:
3874 1157 1156
ApoI r'AATT_y
Cuts at: 1493 2140 2461 2851 2878 3414 3438 3496 3722
Size: 647 321 390 27 536 24 58 226
Cuts at: 3722 3874 5983 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 647 536 390 321 226 152
58 27 24
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
AvrII C'CTAG_G
Cuts at: 3119 3119
Size: 6187
BamHI G'GATC_C
Cuts at: 3202 3517 3884 3202
Size: 315 367 5505
Fragments arranged by size:
5505 367 315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3224 3508 4963 618
Size: 325 217 627 1437 284 1455 1842
Fragments arranged by size:
1842 1455 1437 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 3348 3506 1391
Size: 1957 158 4072
Fragments arranged by size:
4072 1957 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2640 3396 3446 928
Size: 971 741 756 50 3669
Fragments arranged by size:
3669 971 756 741 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2432 4079 5289 2432
Size: 1647 1210 3330
Fragments arranged by size:
3330 1647 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2022 2317 6182 2022
Size: 295 3865 2027
Fragments arranged by size:
3865 2027 295
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 3091 3229 4248 5404 1790
Size: 1301 138 1019 1156 2573
Fragments arranged by size:
2573 1301 1156 1019 138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2617 3918 4876
Size: 416 53 83 399 24 1200 1301 958
Cuts at: 4876 442
Size: 1753
Fragments arranged by size:
1753 1301 1200 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 4847 1239
Size: 1305 2303 2579
Fragments arranged by size:
2579 2303 1305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2730 2760 1178
Size: 30 165 30 1327 30 4605
Fragments arranged by size:
4605 1327 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2950 2953 1186
Size: 1764 3 4420
Fragments arranged by size:
4420 1764 3
BseYI C'CCAG_C
Cuts at: 5390 5886 5390
Size: 496 5691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 2600 1163
Size: 573 322 542 4750
Fragments arranged by size:
4750 573 542 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2607 2722 3091 4248 4333 5404 2607
Size: 115 369 1157 85 1071 3390
Fragments arranged by size:
3390 1157 1071 369 115 85
BsmI GAATG_Cn'
Cuts at: 3014 3285 3698 3791 3014
Size: 271 413 93 5410
Fragments arranged by size:
5410 413 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2607 2722 3091 3229 3348 3506 4248
Size: 399 817 115 369 138 119 158 742
Cuts at: 4248 4333 5404 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 817 742 399 369 158 138
119 115 85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2899 3542 844
Size: 2055 643 3489
Fragments arranged by size:
3489 2055 643
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 4682 4856 65
Size: 1183 822 2612 174 1396
Fragments arranged by size:
2612 1396 1183 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 3185 4247 5259 3185
Size: 1062 1012 4113
Fragments arranged by size:
4113 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2675 794
Size: 1881 4306
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2786 2786
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2022 2317 2625 3139 3202 3460 3517 3884
Size: 50 295 308 514 63 258 57 367
Cuts at: 3884 4269 4286 5738 5824 5836 6182 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 514 385 367 346 308 295
258 86 63 57 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DraIII CAC_nnn'GTG
Cuts at: 3252 3252
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152
Size: 54 1425 50 275 18 1804 889 1629
Cuts at: 6152 8
Size: 43
Fragments arranged by size:
1804 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2745 4117 1353
Size: 1392 1372 3423
Fragments arranged by size:
3423 1392 1372
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2544 2634 4250 4847 5634 5902
Size: 393 710 202 90 1616 597 787 268
Cuts at: 5902 1239
Size: 1524
Fragments arranged by size:
1616 1524 787 710 597 393 268 202
90
EcoO109I rG'GnC_Cy
Cuts at: 2054 2515 2584 2054
Size: 461 69 5657
Fragments arranged by size:
5657 461 69
EcoRI G'AATT_C
Cuts at: 2140 3414 3496 3874 2140
Size: 1274 82 378 4453
Fragments arranged by size:
4453 1274 378 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2270 3063 3328 2270
Size: 793 265 5129
Fragments arranged by size:
5129 793 265
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2610 2642 3161 3193 3318
Size: 32 135 32 1038 32 519 32 125
Cuts at: 3318 3350 4828 4860 4902 4934 1373
Size: 32 1478 32 42 32 2626
Fragments arranged by size:
2626 1478 1038 519 135 125 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2334 748
Size: 1586 4601
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3387 3786 3387
Size: 399 5788
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2029 2303 2502 5301 5485 1305
Size: 724 274 199 2799 184 2007
Fragments arranged by size:
2799 2007 724 274 199 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2918 3102 3153 4145 4504 4663 518
Size: 648 1752 184 51 992 359 159 2042
Fragments arranged by size:
2042 1752 992 648 359 184 159 51
MspA1I CmG'CkG
Cuts at: 1606 3603 4280 5428 5673 1606
Size: 1997 677 1148 245 2120
Fragments arranged by size:
2120 1997 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 2079 3208 3421 387
Size: 1692 1129 213 3153
Fragments arranged by size:
3153 1692 1129 213
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2064 3219 3262 3545 5090 1132
Size: 97 835 1155 43 283 1545 2229
Fragments arranged by size:
2229 1545 1155 835 283 97 43
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 2060 3258 5086 2060
Size: 1198 1828 3161
Fragments arranged by size:
3161 1828 1198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2951 3336 2951
Size: 385 5802
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2515 2054
Size: 461 5726
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 3539 830
Size: 2709 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 2538 4435 6147 2538
Size: 1897 1712 2578
Fragments arranged by size:
2578 1897 1712
SfcI C'TryA_G
Cuts at: 826 1045 3535 4670 5351 5542 826
Size: 219 2490 1135 681 191 1471
Fragments arranged by size:
2490 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 2227 3071 4111 5
Size: 47 2175 844 1040 2081
Fragments arranged by size:
2175 2081 1040 844 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 3119 3198 3626 513
Size: 552 2054 79 428 3074
Fragments arranged by size:
3074 2054 552 428 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4231 4384 4569 5014 325
Size: 1255 2634 17 153 185 445 1498
Fragments arranged by size:
2634 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2536 4433 6145
Size: 274 80 33 51 1443 559 1897 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1897 1712 1443 559 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 3285 4316 3285
Size: 1031 5156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI