Restriction map for
pFN21AB5479
(Circular) (Six-base) MAPSORT of: pFN21AB5479.seq Check: 1930 from: 1 to: 6187
pFN21AB5479 6187 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 2407 2966 3508 2407
Size: 559 542 5086
Fragments arranged by size:
5086 559 542
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2564 2856 3243 4250 5634 5902 1632
Size: 801 131 292 387 1007 1384 268 1917
Fragments arranged by size:
1917 1384 1007 801 387 292 268 131
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 2616 1166
Size: 1450 4737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2963 1184
Size: 1779 4408
AlwNI CAG_nnn'CTG
Cuts at: 2470 2605 3930 5502 2470
Size: 135 1325 1572 3155
Fragments arranged by size:
3155 1572 1325 135
ApaLI G'TGCA_C
Cuts at: 2609 4244 5400 2609
Size: 1635 1156 3396
Fragments arranged by size:
3396 1635 1156
ApoI r'AATT_y
Cuts at: 1493 3496 3722 3874 5983 1493
Size: 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3282 3512 1870
Size: 73 1339 230 4545
Fragments arranged by size:
4545 1339 230 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2425 2507 2966 3195
Size: 325 217 627 620 18 82 459 229
Cuts at: 3195 3299 3508 4963 618
Size: 104 209 1455 1842
Fragments arranged by size:
1842 1455 627 620 459 325 229 217
209 104 82 18
BanII G_rGCy'C
Cuts at: 1391 2639 3506 1391
Size: 1248 867 4072
Fragments arranged by size:
4072 1248 867
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2844 3421 928
Size: 971 945 577 3694
Fragments arranged by size:
3694 971 945 577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2471 4079 5289 2471
Size: 1608 1210 3369
Fragments arranged by size:
3369 1608 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3085 4798 136
Size: 107 122 71 631 605 1413 1713 1525
Fragments arranged by size:
1713 1525 1413 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2769 6182 2769
Size: 3413 2774
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2613 2749 2767 3095 3304 3316 4248 5404
Size: 823 136 18 328 209 12 932 1156
Cuts at: 5404 1790
Size: 2573
Fragments arranged by size:
2573 1156 932 823 328 209 136 18
12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3918 4876
Size: 416 53 83 399 24 762 1739 958
Cuts at: 4876 442
Size: 1753
Fragments arranged by size:
1753 1739 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2334 2514 2334
Size: 180 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2146 2269 2312 2484 4847 1239
Size: 878 29 123 43 172 2363 2579
Fragments arranged by size:
2579 2363 878 172 123 43 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 3311 4312 5177 5475 2681
Size: 630 1001 865 298 3393
Fragments arranged by size:
3393 1001 865 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3484 3978 493
Size: 711 2280 494 2702
Fragments arranged by size:
2702 2280 711 494
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2307 2988 4620 5018 5292 5439 5850 2307
Size: 681 1632 398 274 147 411 2644
Fragments arranged by size:
2644 1632 681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3068 3098 1178
Size: 30 165 30 1665 30 4267
Fragments arranged by size:
4267 1665 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3086 3089 1186
Size: 1900 3 4284
Fragments arranged by size:
4284 1900 3
BseYI C'CCAG_C
Cuts at: 2026 2171 2397 5390 5886 2026
Size: 145 226 2993 496 2327
Fragments arranged by size:
2993 2327 496 226 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3024 3251 3420 1163
Size: 573 1288 227 169 3930
Fragments arranged by size:
3930 1288 573 227 169
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2613 2639 4248 4333 5404 2613
Size: 26 1609 85 1071 3396
Fragments arranged by size:
3396 1609 1071 85 26
BsmI GAATG_Cn'
Cuts at: 2998 3698 3791 2998
Size: 700 93 5394
Fragments arranged by size:
5394 700 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2613 2639 2749 2767 3095 3304 3316
Size: 399 823 26 110 18 328 209 12
Cuts at: 3316 3506 4248 4333 5404 1391
Size: 190 742 85 1071 2174
Fragments arranged by size:
2174 1071 823 742 399 328 209 190
110 85 26 18 12
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 3033 4076 5806 3033
Size: 1043 1730 3414
Fragments arranged by size:
3414 1730 1043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2138 2850 3542 844
Size: 1294 712 692 3489
Fragments arranged by size:
3489 1294 712 692
BsrBI CCG'CTC
Cuts at: 1394 1823 2388 4074 1394
Size: 429 565 1686 3507
Fragments arranged by size:
3507 1686 565 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2736 4682 4856 65
Size: 1183 1488 1946 174 1396
Fragments arranged by size:
1946 1488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 2007 4991 2007
Size: 2984 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2935 2941 3343 2056
Size: 879 6 402 4900
Fragments arranged by size:
4900 879 402 6
BstYI r'GATC_y
Cuts at: 1972 2133 2247 2276 2769 3517 3884 4269 4286
Size: 161 114 29 493 748 367 385 17
Cuts at: 4286 5738 5824 5836 6182 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 748 493 385 367 346 161
114 86 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2846 2934 3342 513
Size: 552 1781 88 408 3358
Fragments arranged by size:
3358 1781 552 408 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2082 3136 487
Size: 683 56 856 1054 3538
Fragments arranged by size:
3538 1054 856 683 56
BtsI GCAGTG_nn'
Cuts at: 2593 2915 3095 3703 4497 4517 2593
Size: 322 180 608 794 20 4263
Fragments arranged by size:
4263 794 608 322 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DraIII CAC_nnn'GTG
Cuts at: 2187 2187
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3124 3634
Size: 54 1425 50 275 18 216 1078 510
Cuts at: 3634 4523 6152 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1078 889 510 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2409 2429 4117 1353
Size: 1056 20 1688 3423
Fragments arranged by size:
3423 1688 1056 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2637 2637
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2146 2269 2312 2433 2484 2564
Size: 393 485 29 123 43 121 51 80
Cuts at: 2564 2856 3243 4250 4847 5634 5902 1239
Size: 292 387 1007 597 787 268 1524
Fragments arranged by size:
1524 1007 787 597 485 393 387 292
268 123 121 80 51 43 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2546 2546
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 2224 2419 3001 3359 2224
Size: 195 582 358 5052
Fragments arranged by size:
5052 582 358 195
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 3184 4693 3184
Size: 1509 4678
HaeII r_GCGC'y
Cuts at: 1678 2197 5334 1678
Size: 519 3137 2531
Fragments arranged by size:
3137 2531 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2775 2807 2813 2845 4828
Size: 32 135 32 1203 32 6 32 1983
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1983 1203 135 42 32 32 32
32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 3531 3777 669
Size: 988 281 124 1469 246 3079
Fragments arranged by size:
3079 1469 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 2411 2970 3512 2411
Size: 559 542 5086
Fragments arranged by size:
5086 559 542
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5301 5485 1305
Size: 3996 184 2007
Fragments arranged by size:
3996 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 4149
Fragments arranged by size:
4149 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2616 2618 2939 2945 4145 4504
Size: 648 1368 82 2 321 6 1200 359
Cuts at: 4504 4663 518
Size: 159 2042
Fragments arranged by size:
2042 1368 1200 648 359 321 159 82
6 2
MspA1I CmG'CkG
Cuts at: 1606 2175 2470 3603 4280 5428 5673 1606
Size: 569 295 1133 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 677 569 295 245
NcoI C'CATG_G
Cuts at: 513 1065 2934 3342 513
Size: 552 1869 408 3358
Fragments arranged by size:
3358 1869 552 408
NdeI CA'TA_TG
Cuts at: 387 3474 387
Size: 3087 3100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2044 2168 2533 3545 5090 1132
Size: 97 815 124 365 1012 1545 2229
Fragments arranged by size:
2229 1545 1012 815 365 124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2095 2356 2470 2605 1108
Size: 987 261 114 135 4690
Fragments arranged by size:
4690 987 261 135 114
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2419 3001 3359 2224
Size: 195 582 358 5052
Fragments arranged by size:
5052 582 358 195
PshAI GACnn'nnGTC
Cuts at: 1613 2091 2583 1613
Size: 478 492 5217
Fragments arranged by size:
5217 492 478
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspXI vC'TCGA_Gb
Cuts at: 3282 3282
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2149 3280 3539 830
Size: 1319 1131 259 3478
Fragments arranged by size:
3478 1319 1131 259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 2470 2470
Size: 6187
SacI G_AGCT'C
Cuts at: 2639 2639
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 3001 3001
Size: 6187
SapI GCTCTTCn'nnn_
Cuts at: 2429 2429
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2145 3276 3535 4670 5351 5542 826
Size: 219 1100 1131 259 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 1100 681 259 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3282 3326 4291 5192 5454
Size: 197 926 753 586 44 965 901 262
Cuts at: 5454 820
Size: 1553
Fragments arranged by size:
1553 965 926 901 753 586 262 197
44
SnaBI TAC'GTA
Cuts at: 493 3484 3978 493
Size: 2991 494 2702
Fragments arranged by size:
2991 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 3293 1517
Size: 1776 4411
StyI C'CwwG_G
Cuts at: 513 1065 2640 2730 2928 2934 3342 3626 513
Size: 552 1575 90 198 6 408 284 3074
Fragments arranged by size:
3074 1575 552 408 284 198 90 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2198 4214 4231 4384 4569 5014 325
Size: 1255 618 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 618 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3065 4433 6145
Size: 274 80 33 51 1443 1088 1368 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1712 1443 1368 1088 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 6147 6179 3068
Size: 32 3047 32 3076
Fragments arranged by size:
3076 3047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2508 2501
Size: 7 6180
XhoI C'TCGA_G
Cuts at: 1943 3282 1943
Size: 1339 4848
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI