Restriction map for
pFN21AB5477
(Circular) (Six-base) MAPSORT of: pFN21AB5477.seq Check: 5682 from: 1 to: 5878
pFN21AB5477 5878 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 2653 4005 4378 1563
Size: 1090 1352 373 3063
Fragments arranged by size:
3063 1352 1090 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 3120 3941 5325 5593 1632
Size: 710 778 821 1384 268 1917
Fragments arranged by size:
1917 1384 821 778 710 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 3063 4777 5754 1203
Size: 1860 1714 977 1327
Fragments arranged by size:
1860 1714 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 2454 4607 2454
Size: 2153 3725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 5846
AlwNI CAG_nnn'CTG
Cuts at: 2320 3621 5193 2320
Size: 1301 1572 3005
Fragments arranged by size:
3005 1572 1301
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3187 3413 3565 5674 1493
Size: 1694 226 152 2109 1697
Fragments arranged by size:
2109 1697 1694 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2412 2870 3203 1870
Size: 73 469 458 333 4545
Fragments arranged by size:
4545 469 458 333 73
BamHI G'GATC_C
Cuts at: 2272 2407 2472 3208 3575 2272
Size: 135 65 736 367 4575
Fragments arranged by size:
4575 736 367 135 65
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 3005 3199 4654 618
Size: 325 217 627 612 606 194 1455 1842
Fragments arranged by size:
1842 1455 627 612 606 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2260 3096 3197 1391
Size: 869 836 101 4072
Fragments arranged by size:
4072 869 836 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 2736 2864 2963 928
Size: 971 621 216 128 99 3843
Fragments arranged by size:
3843 971 621 216 128 99
BbvCI CC'TCA_GC
Cuts at: 2706 2706
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4489 136
Size: 107 122 71 631 605 337 2480 1525
Fragments arranged by size:
2480 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 2103 3295 2103
Size: 1192 4686
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2584 3939 5095 1790
Size: 614 180 1355 1156 2573
Fragments arranged by size:
2573 1355 1156 614 180
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2630 4538 1239
Size: 1169 222 1908 2579
Fragments arranged by size:
2579 1908 1169 222
Bpu10I CC'TnA_GC
Cuts at: 2706 2905 2706
Size: 199 5679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4003 4868 5166 4003
Size: 865 298 4715
Fragments arranged by size:
4715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2141 2207 4311 4709 4983 5130 5541 2141
Size: 66 2104 398 274 147 411 2478
Fragments arranged by size:
2478 2104 411 398 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2867 2897 3155 3185 1178
Size: 30 165 30 1464 30 258 30 3871
Fragments arranged by size:
3871 1464 258 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2723 2726 2783 2888 3163 1186
Size: 1537 3 57 105 275 3901
Fragments arranged by size:
3901 1537 275 105 57 3
BseYI C'CCAG_C
Cuts at: 5081 5577 5081
Size: 496 5382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3328 4089 5117 1540
Size: 275 186 1051 276 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 276 275 186
BsiHKAI G_wGCw'C
Cuts at: 3939 4024 5095 3939
Size: 85 1071 4722
Fragments arranged by size:
4722 1071 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 2404 2584 3096 3197 3939 4024
Size: 399 470 144 180 512 101 742 85
Cuts at: 4024 5095 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 512 470 399 180 144
101 85
BspEI T'CCGG_A
Cuts at: 2207 5541 2207
Size: 3334 2544
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3233 844
Size: 2389 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2904 3765 1394
Size: 429 1081 861 3507
Fragments arranged by size:
3507 1081 861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2982 2991 3000 794
Size: 2188 9 9 3672
Fragments arranged by size:
3672 2188 9 9
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2272 2407 2472 3208 3575 3960 3977 5429
Size: 300 135 65 736 367 385 17 1452
Cuts at: 5429 5515 5527 5873 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 736 385 367 346 300 135
86 65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2268 2268
Size: 5878
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 513
Size: 552 939 60 4327
Fragments arranged by size:
4327 939 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 2584 2958 2978 3394 4188 4208 2078
Size: 506 374 20 416 794 20 3748
Fragments arranged by size:
3748 794 506 416 374 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2190 4885 809
Size: 507 874 2695 1802
Fragments arranged by size:
2695 1802 874 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2899 3325 4214
Size: 54 1425 50 275 18 1069 426 889
Cuts at: 4214 5843 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1069 889 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3038 3808 1353
Size: 1685 770 3423
Fragments arranged by size:
3423 1685 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 4465 4839 4985 387
Size: 1064 138 668 2208 374 146 1280
Fragments arranged by size:
2208 1280 1064 668 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2408 2630 3120 3941 4538 5325
Size: 393 710 66 222 490 821 597 787
Cuts at: 5325 5593 1239
Size: 268 1524
Fragments arranged by size:
1524 821 787 710 597 490 393 268
222 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5878
EcoO109I rG'GnC_Cy
Cuts at: 2789 2789
Size: 5878
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FspI TGC'GCA
Cuts at: 2375 4384 2375
Size: 2009 3869
HaeII r_GCGC'y
Cuts at: 1678 2239 5025 1678
Size: 561 2786 2531
Fragments arranged by size:
2786 2531 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 2658 2690 2762
Size: 32 135 32 868 32 186 32 72
Cuts at: 2762 2794 2867 2899 4519 4551 4593 4625 1373
Size: 32 73 32 1620 32 42 32 2626
Fragments arranged by size:
2626 1620 868 186 135 73 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3147 748
Size: 2399 3479
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 2158 3477 2158
Size: 1319 4559
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3089 4992 5176 1305
Size: 1784 1903 184 2007
Fragments arranged by size:
2007 1903 1784 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 3836 4195 4354 518
Size: 648 1005 1665 359 159 2042
Fragments arranged by size:
2042 1665 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 2967 3294 3971 5119 5364 1606
Size: 609 752 327 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 609 327 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2064 513
Size: 552 939 60 4327
Fragments arranged by size:
4327 939 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 3067 3236 4781 1132
Size: 97 1838 169 1545 2229
Fragments arranged by size:
2229 1838 1545 169 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 3063 4777 3063
Size: 1714 4164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2194 2493 2607 2194
Size: 299 114 5465
Fragments arranged by size:
5465 299 114
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3947 3979 5460 5492 3155
Size: 32 760 32 1481 32 3541
Fragments arranged by size:
3541 1481 760 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2789 2789
Size: 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 3052 2001
Size: 1051 4827
PvuII CAG'CTG
Cuts at: 2215 2215
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SapI GCTCTTCn'nnn_
Cuts at: 3038 3038
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 2051 4126 5838 2051
Size: 2075 1712 2091
Fragments arranged by size:
2091 2075 1712
SfcI C'TryA_G
Cuts at: 826 1045 3226 4361 5042 5233 826
Size: 219 2181 1135 681 191 1471
Fragments arranged by size:
2181 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3982 4883 5145 820
Size: 197 926 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 1517
Size: 1156 4722
StyI C'CwwG_G
Cuts at: 513 1065 2004 2038 2064 2559 3317 513
Size: 552 939 34 26 495 758 3074
Fragments arranged by size:
3074 939 758 552 495 34 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2758 3905 3922 4075 4260 4705 325
Size: 1255 1178 1147 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1178 1147 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 2217 3066
Size: 274 80 33 51 1443 72 168 849
Cuts at: 3066 4124 5836 96
Size: 1058 1712 138
Fragments arranged by size:
1712 1443 1058 849 274 168 138 80
72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI