Restriction map for
pFN21AB5464
(Circular) (Six-base) MAPSORT of: pFN21AB5464.seq Check: 3431 from: 1 to: 5503
pFN21AB5464 5503 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2192
Size: 5503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2137 2824 2137
Size: 687 4816
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4950 5218 1632
Size: 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 2259 4402 5379 1203
Size: 1056 2143 977 1327
Fragments arranged by size:
2143 1327 1056 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 3246 4818 3246
Size: 1572 3931
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2387 2774 2812 3038 3190 5299 1493
Size: 894 387 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 387 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 1870
Size: 73 885 4545
Fragments arranged by size:
4545 885 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2824 4279 618
Size: 325 217 627 350 687 1455 1842
Fragments arranged by size:
1842 1455 687 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2313 2822 1391
Size: 673 249 509 4072
Fragments arranged by size:
4072 673 509 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2731 2765 3694 3728 1903
Size: 34 794 34 929 34 3678
Fragments arranged by size:
3678 929 794 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 2641 1548
Size: 1093 4410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2476 5498 2476
Size: 3022 2481
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 3564 4720 1790
Size: 1774 1156 2573
Fragments arranged by size:
2573 1774 1156
BmgBI CAC'GTC
Cuts at: 1318 2260 1318
Size: 942 4561
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2567 4163 1239
Size: 1328 1596 2579
Fragments arranged by size:
2579 1596 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4166 882
Size: 617 2667 2219
Fragments arranged by size:
2667 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3294 493
Size: 711 2090 2702
Fragments arranged by size:
2702 2090 711
BsaBI GATnn'nnATC
Cuts at: 2424 5497 2424
Size: 3073 2430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 3310 3692 4327
Size: 53 83 186 936 567 1210 382 635
Cuts at: 4327 275
Size: 1451
Fragments arranged by size:
1451 1210 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2232 1186
Size: 904 142 4457
Fragments arranged by size:
4457 904 142
BseYI C'CCAG_C
Cuts at: 2326 4706 5202 2326
Size: 2380 496 2627
Fragments arranged by size:
2627 2380 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4930
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 3564 3649 4720 2610
Size: 954 85 1071 3393
Fragments arranged by size:
3393 1071 954 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2313 2610 2822 3564 3649 4720
Size: 399 274 249 297 212 742 85 1071
Cuts at: 4720 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 297 274 249 212
85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 2443 2602 2674 3392 5122 2443
Size: 159 72 718 1730 2824
Fragments arranged by size:
2824 1730 718 159 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2858 844
Size: 1348 666 3489
Fragments arranged by size:
3489 1348 666
BsrBI CCG'CTC
Cuts at: 1394 1823 2030 2070 3390 1394
Size: 429 207 40 1320 3507
Fragments arranged by size:
3507 1320 429 207 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 2229 96
Size: 2133 3370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2476 2833 3200 3585 3602 5054 5140 5152
Size: 504 357 367 385 17 1452 86 12
Cuts at: 5152 5498 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 504 385 367 357 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3235
Fragments arranged by size:
3235 939 777 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 2950 3839
Size: 54 1425 50 275 18 316 804 889
Cuts at: 3839 5468 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 804 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2149 3433 1353
Size: 796 1284 3423
Fragments arranged by size:
3423 1284 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4090 4464 4610 387
Size: 1064 138 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 3566 4163 4950 5218 1239
Size: 393 935 999 597 787 268 1524
Fragments arranged by size:
1524 999 935 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2122 2122
Size: 5503
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 4009 4009
Size: 5503
HaeII r_GCGC'y
Cuts at: 1678 2084 4650 1678
Size: 406 2566 2531
Fragments arranged by size:
2566 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373
Size: 32 135 32 2572 32 42 32 2626
Fragments arranged by size:
2626 2572 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2847 3093 669
Size: 988 281 909 246 3079
Fragments arranged by size:
3079 988 909 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2141 2828 2141
Size: 687 4816
MfeI C'AATT_G
Cuts at: 2346 2743 3102 2346
Size: 397 359 4747
Fragments arranged by size:
4747 397 359
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2148 10
Size: 54 1425 343 316 3365
Fragments arranged by size:
3365 1425 343 316 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3461 3820 3979 518
Size: 648 1359 936 359 159 2042
Fragments arranged by size:
2042 1359 936 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 2919 3596 4744 4989 1606
Size: 724 589 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 589 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4012
Fragments arranged by size:
4012 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2176 2861 4406 1132
Size: 97 947 685 1545 2229
Fragments arranged by size:
2229 1545 947 685 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2122
Size: 5503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PstI C_TGCA'G
Cuts at: 830 2320 2855 830
Size: 1490 535 3478
Fragments arranged by size:
3478 1490 535
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
PvuII CAG'CTG
Cuts at: 2330 2330
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SapI GCTCTTCn'nnn_
Cuts at: 2149 2149
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2316 2851 3986 4667 4858 826
Size: 219 1271 535 1135 681 191 1471
Fragments arranged by size:
1471 1271 1135 681 535 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 4508 4770 820
Size: 197 926 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 2237 2359 3427 5
Size: 47 2185 122 1068 2081
Fragments arranged by size:
2185 2081 1068 122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2004 2942 513
Size: 552 939 938 3074
Fragments arranged by size:
3074 939 938 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2548 3530 3547 3700 3885 4330 325
Size: 1255 968 982 17 153 185 445 1498
Fragments arranged by size:
1498 1255 982 968 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 2262 2280
Size: 274 80 33 51 1443 252 33 18
Cuts at: 2280 3749 5461 96
Size: 1469 1712 138
Fragments arranged by size:
1712 1469 1443 274 252 138 80 51
33 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 2102 2670 1316
Size: 786 568 4149
Fragments arranged by size:
4149 786 568
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 2542
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI