Restriction map for
pFN21AB5463
(Circular) (Six-base) MAPSORT of: pFN21AB5463.seq Check: 6009 from: 1 to: 5569
pFN21AB5463 5569 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2101 2101
Size: 5569
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 2912 1937
Size: 790 185 4594
Fragments arranged by size:
4594 790 185
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3632 5016 5284 1632
Size: 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1384 268
AfeI AGC'GCT
Cuts at: 2435 2435
Size: 5569
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 2646 4468 5445 1203
Size: 1443 1822 977 1327
Fragments arranged by size:
1822 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 5537
AlwNI CAG_nnn'CTG
Cuts at: 2578 3312 4884 2578
Size: 734 1572 3263
Fragments arranged by size:
3263 1572 734
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AscI GG'CGCG_CC
Cuts at: 2786 2786
Size: 5569
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 1870
Size: 73 951 4545
Fragments arranged by size:
4545 951 73
BamHI G'GATC_C
Cuts at: 2346 2899 3266 2346
Size: 553 367 4649
Fragments arranged by size:
4649 553 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 4345 618
Size: 325 217 627 1103 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2888 1391
Size: 637 860 4072
Fragments arranged by size:
4072 860 637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2219 2261 2360 928
Size: 971 320 42 99 4137
Fragments arranged by size:
4137 971 320 99 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2821 2855 3760 3794 1903
Size: 34 884 34 905 34 3678
Fragments arranged by size:
3678 905 884 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 2805 1548
Size: 1257 4312
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 4180 136
Size: 107 122 71 631 605 517 1991 1525
Fragments arranged by size:
1991 1525 631 605 517 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 3630 4786 1790
Size: 1840 1156 2573
Fragments arranged by size:
2573 1840 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 4258 442
Size: 416 53 83 399 24 1883 958 1753
Fragments arranged by size:
1883 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2519 2531 2542 2639 4229 1239
Size: 1244 36 12 11 97 1590 2579
Fragments arranged by size:
2579 1590 1244 97 36 12 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2076 4232 882
Size: 617 577 2156 2219
Fragments arranged by size:
2219 2156 617 577
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 2810 5563 2810
Size: 2753 2816
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3758 4393 275
Size: 53 83 186 936 1843 382 635 1451
Fragments arranged by size:
1843 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2349 4002 4400 4674 4821 5232 2349
Size: 1653 398 274 147 411 2686
Fragments arranged by size:
2686 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2381 2411 1178
Size: 30 165 30 912 30 36 30 4336
Fragments arranged by size:
4336 912 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2174 2237 2320 2507 1186
Size: 946 42 63 83 187 4248
Fragments arranged by size:
4248 946 187 83 63 42
BseYI C'CCAG_C
Cuts at: 4772 5268 4772
Size: 496 5073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 3630 3715 4786 2028
Size: 1602 85 1071 2811
Fragments arranged by size:
2811 1602 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
BsmBI CGTCTCn'nnnn_
Cuts at: 2585 2585
Size: 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2888 3630 3715 4786 1391
Size: 399 238 860 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 742 399 238 85
BspEI T'CCGG_A
Cuts at: 2349 5232 2349
Size: 2883 2686
BspHI T'CATG_A
Cuts at: 2802 3458 5188 2802
Size: 656 1730 3183
Fragments arranged by size:
3183 1730 656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2101 2924 844
Size: 1257 823 3489
Fragments arranged by size:
3489 1257 823
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 3456 1394
Size: 429 505 1128 3507
Fragments arranged by size:
3507 1128 505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2471 4213 4400 1700
Size: 110 661 1742 187 2869
Fragments arranged by size:
2869 1742 661 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 2786 1925
Size: 861 4708
BssSI C'ACGA_G
Cuts at: 2669 3629 4641 2669
Size: 960 1012 3597
Fragments arranged by size:
3597 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstXI CCAn_nnnn'nTGG
Cuts at: 2671 2671
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2346 2899 3266 3651 3668 5120 5206 5218
Size: 374 553 367 385 17 1452 86 12
Cuts at: 5218 5564 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 553 385 374 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2342 2342
Size: 5569
BtgI C'CryG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4009
Fragments arranged by size:
4009 1008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2061 2824 487
Size: 683 56 835 763 3232
Fragments arranged by size:
3232 835 763 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2661 3016
Size: 54 1425 50 275 18 204 627 355
Cuts at: 3016 3905 5534 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 355 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2026
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2519 2531 2542 2639 3632 4229
Size: 393 851 36 12 11 97 993 597
Cuts at: 4229 5016 5284 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 851 787 597 393 268 97
36 12 11
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2437 4716 1678
Size: 759 2279 2531
Fragments arranged by size:
2531 2279 759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 4210 4242 4284
Size: 32 135 32 809 32 1797 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 809 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 2379 2445 4683 4867 1305
Size: 963 111 66 2238 184 2007
Fragments arranged by size:
2238 2007 963 184 111 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 10
Size: 54 1425 343 831 2916
Fragments arranged by size:
2916 1425 831 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 2657 2663 2669 3527 3886 4045
Size: 648 1485 6 6 6 858 359 159
Cuts at: 4045 518
Size: 2042
Fragments arranged by size:
2042 1485 858 648 359 159 6 6
6
MspA1I CmG'CkG
Cuts at: 1606 2336 2461 2985 3662 4810 5055 1606
Size: 730 125 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 524 245 125
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2509 2650 2927 4472 1132
Size: 97 1280 141 277 1545 2229
Fragments arranged by size:
2229 1545 1280 277 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 2646 4468 2646
Size: 1822 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 3638 3670 5151 5183 2315
Size: 32 1291 32 1481 32 2701
Fragments arranged by size:
2701 1481 1291 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2921 830
Size: 2091 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5569
SacI G_AGCT'C
Cuts at: 2028 2028
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2917 4052 4733 4924 826
Size: 219 1872 1135 681 191 1471
Fragments arranged by size:
1872 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 1517
Size: 802 4767
StyI C'CwwG_G
Cuts at: 513 1065 2037 3008 513
Size: 552 972 971 3074
Fragments arranged by size:
3074 972 971 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 2094 3596 3613 3766 3951 4396
Size: 1255 448 66 1502 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1502 1498 1255 448 445 185 153 66
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 2706 3815
Size: 274 80 33 51 1443 426 303 1109
Cuts at: 3815 5527 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 426 303 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2164 2196 5529 5561 2164
Size: 32 3333 32 2172
Fragments arranged by size:
3333 2172 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2494 2383
Size: 111 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI