Restriction map for
pFN21AB5457
(Circular) (Six-base) MAPSORT of: pFN21AB5457.seq Check: 9491 from: 1 to: 6154
pFN21AB5457 6154 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2760 2760
Size: 6154
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 2269 3475 2269
Size: 1206 4948
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2903 2936 3146 4217 5601 5869 1632
Size: 587 684 33 210 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 684 587 268 210 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2768 3278 1184
Size: 1584 510 4060
Fragments arranged by size:
4060 1584 510
AlwNI CAG_nnn'CTG
Cuts at: 2176 3897 5469 2176
Size: 1721 1572 2861
Fragments arranged by size:
2861 1721 1572
ApaI G_GGCC'C
Cuts at: 2410 2410
Size: 6154
ApaLI G'TGCA_C
Cuts at: 2561 4211 5367 2561
Size: 1650 1156 3348
Fragments arranged by size:
3348 1650 1156
ApoI r'AATT_y
Cuts at: 1493 2311 2364 2895 3463 3689 3841 5950 1493
Size: 818 53 531 568 226 152 2109 1697
Fragments arranged by size:
2109 1697 818 568 531 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 3479 1870
Size: 73 1536 4545
Fragments arranged by size:
4545 1536 73
AvrII C'CTAG_G
Cuts at: 3121 3121
Size: 6154
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2261 2294 2261
Size: 33 6121
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2269 2340 3425 3475
Size: 325 217 627 378 104 71 1085 50
Cuts at: 3475 4930 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1085 627 378 325 217 104
71 50
BanII G_rGCy'C
Cuts at: 1391 2410 3361 3473 1391
Size: 1019 951 112 4072
Fragments arranged by size:
4072 1019 951 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2275 4046 5256 2275
Size: 1771 1210 3173
Fragments arranged by size:
3173 1771 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BfrBI ATG'CAT
Cuts at: 3140 3140
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 3423 4765
Size: 107 122 71 631 605 469 1282 1342
Cuts at: 4765 136
Size: 1525
Fragments arranged by size:
1525 1342 1282 631 605 469 122 107
71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3339 3571 3339
Size: 232 5922
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2410 2565 2792 4215 5371 1790
Size: 380 240 155 227 1423 1156 2573
Fragments arranged by size:
2573 1423 1156 380 240 227 155
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4846
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2530 3249
Size: 416 53 83 399 24 747 366 719
Cuts at: 3249 3885 4843 442
Size: 636 958 1753
Fragments arranged by size:
1753 958 747 719 636 416 399 366
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2907 1056
Size: 1851 4303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2707 2206
Size: 501 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2523 2984 4814 1239
Size: 1284 461 1830 2579
Fragments arranged by size:
2579 1830 1284 461
Bpu10I CC'TnA_GC
Cuts at: 3029 3029
Size: 6154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2708 2997 4279 5144 5442 2336
Size: 372 289 1282 865 298 3048
Fragments arranged by size:
3048 1282 865 372 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2194 2340 4817 882
Size: 617 695 146 2477 2219
Fragments arranged by size:
2477 2219 695 617 146
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 2705 6148 2705
Size: 3443 2711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2841 2871 1178
Size: 30 165 30 1438 30 4461
Fragments arranged by size:
4461 1438 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6154
BseYI C'CCAG_C
Cuts at: 2234 2629 5357 5853 2234
Size: 395 2728 496 2535
Fragments arranged by size:
2728 2535 496 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2565 4215 4300 5371 2565
Size: 1650 85 1071 3348
Fragments arranged by size:
3348 1650 1071 85
BsmI GAATG_Cn'
Cuts at: 3255 3665 3758 3255
Size: 410 93 5651
Fragments arranged by size:
5651 410 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2410 2565 2792 3361 3473 4215
Size: 399 380 240 155 227 569 112 742
Cuts at: 4215 4300 5371 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 380 240 227
155 112 85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3509 844
Size: 1916 749 3489
Fragments arranged by size:
3489 1916 749
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 4041 1394
Size: 429 559 1659 3507
Fragments arranged by size:
3507 1659 559 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 4649 4823 65
Size: 1183 1233 2168 174 1396
Fragments arranged by size:
2168 1396 1233 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2942 2161
Size: 781 5373
BstYI r'GATC_y
Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149
Size: 1512 367 385 17 1452 86 12 346
Cuts at: 6149 1972
Size: 1977
Fragments arranged by size:
1977 1512 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2077 3418 513
Size: 552 939 73 1341 3249
Fragments arranged by size:
3249 1341 939 552 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2005 2406 3061 487
Size: 683 56 779 401 655 3580
Fragments arranged by size:
3580 779 683 655 401 56
BtsI GCAGTG_nn'
Cuts at: 3276 3670 4464 4484 3276
Size: 394 794 20 4946
Fragments arranged by size:
4946 794 394 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3031 3052 3066 3087 529
Size: 35 2467 21 14 21 3596
Fragments arranged by size:
3596 2467 35 21 21 14
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DraIII CAC_nnn'GTG
Cuts at: 2551 2551
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2133 3378
Size: 54 1425 50 275 18 207 96 1245
Cuts at: 3378 3601 4490 6119 8
Size: 223 889 1629 43
Fragments arranged by size:
1629 1425 1245 889 275 223 207 96
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4084 1353
Size: 2731 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2227 2803 4741 5115 5261 387
Size: 1064 138 638 576 1938 374 146 1280
Fragments arranged by size:
1938 1280 1064 638 576 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2523 2903 2936 2984 3146 4217
Size: 393 587 304 380 33 48 162 1071
Cuts at: 4217 4814 5601 5869 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1071 787 597 587 393 380 304
268 162 48 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 3332 2228
Size: 1104 5050
EcoO109I rG'GnC_Cy
Cuts at: 2406 2921 2406
Size: 515 5639
EcoRI G'AATT_C
Cuts at: 2895 3463 3841 2895
Size: 568 378 5208
Fragments arranged by size:
5208 568 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 6154
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 5301 1678
Size: 3623 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 2841 2873 2939
Size: 32 135 32 453 32 784 32 66
Cuts at: 2939 2971 4795 4827 4869 4901 1373
Size: 32 1824 32 42 32 2626
Fragments arranged by size:
2626 1824 784 453 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3744 669
Size: 988 281 1560 246 3079
Fragments arranged by size:
3079 1560 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 2273 3479 2273
Size: 1206 4948
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 5268 5452 1305
Size: 954 3009 184 2007
Fragments arranged by size:
3009 2007 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 3380 10
Size: 54 1425 343 303 1245 2784
Fragments arranged by size:
2784 1425 1245 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3260 3366 4112 4471 4630 518
Size: 648 2094 106 746 359 159 2042
Fragments arranged by size:
2094 2042 746 648 359 159 106
MspA1I CmG'CkG
Cuts at: 1606 2593 3197 3343 3570 4247 5395 5640 1606
Size: 987 604 146 227 677 1148 245 2120
Fragments arranged by size:
2120 1148 987 677 604 245 227 146
NcoI C'CATG_G
Cuts at: 513 1065 2004 3418 513
Size: 552 939 1414 3249
Fragments arranged by size:
3249 1414 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 2903 1052
Size: 1851 4303
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NsiI A_TGCA'T
Cuts at: 3142 3142
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2590 3512 5057 1132
Size: 97 1361 922 1545 2229
Fragments arranged by size:
2229 1545 1361 922 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PfoI T'CCnGG_A
Cuts at: 2961 2961
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2921 2921
Size: 6154
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2406 2406
Size: 6154
PstI C_TGCA'G
Cuts at: 830 3506 830
Size: 2676 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2593 3197 2593
Size: 604 5550
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SanDI GG'GwC_CC
Cuts at: 2921 2921
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SfcI C'TryA_G
Cuts at: 826 1045 3502 4637 5318 5509 826
Size: 219 2457 1135 681 191 1471
Fragments arranged by size:
2457 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2723 3012 4258 5159 5421
Size: 197 926 408 372 289 1246 901 262
Cuts at: 5421 820
Size: 1553
Fragments arranged by size:
1553 1246 926 901 408 372 289 262
197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 2719 4078 5
Size: 47 2667 1359 2081
Fragments arranged by size:
2667 2081 1359 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 5011
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 2476 3121 3418 3593 513
Size: 552 939 354 118 645 297 175 3074
Fragments arranged by size:
3074 939 645 552 354 297 175 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 2512 4181 4198 4351 4536 4981
Size: 1255 573 359 1669 17 153 185 445
Cuts at: 4981 325
Size: 1498
Fragments arranged by size:
1669 1498 1255 573 445 359 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4400 6112 96
Size: 274 80 33 51 1443 2423 1712 138
Fragments arranged by size:
2423 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2142 2174 6114 6146 2142
Size: 32 3940 32 2150
Fragments arranged by size:
3940 2150 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2738 2815 2738
Size: 77 6077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 2957 4283 2957
Size: 1326 4828
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI